太湖蓝藻多糖应用于重金属离子吸附
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摘要
对太湖蓝藻多糖进行提取,并利用这种多糖对重金属离子进行吸附试验。在蓝藻多糖提取中,采用超声波辅助热水浸提的方法并对浸提时间、浸提温度和料液比条件进行优化,同时考察多糖投加量、吸附时间、pH和温度条件对多糖吸附Pb~(2+)和Cd~(2+)离子的影响。结果表明:在液料比40∶1 mL/g、浸提温度80℃条件下提取5 h多糖得率达到25.1%。在重金属离子吸附研究中发现,当1 000 mL溶液中多糖投加量为100 mg,吸附时间为3 h,pH为7.0,处理温度为25℃时,吸附效果最好,铅、镉的平衡吸附量分别可达到73.81 mg/g和49.69 mg/g,并且Langmuir模型比Freundlich模型更适用于描述蓝藻多糖对Pb~(2+)和Cd~(2+)的吸附平衡。研究表明,太湖蓝藻可作为提取具有污水重金属吸附作用的多糖的来源,从而提高太湖蓝藻的应用潜力和价值。
Polysaccharide was extracted from Cyanobacteria collected from Taihu Lake,and further used to adsorb Pb~(2+) and Cd~(2+).Polysaccharide from Cyanobacteria was extracted with ultrasonic assisted hot water extraction,and extraction conditions,including extraction time,temperature and solid-liquid ratio,were optimized.Meanwhile,effects of adsorption conditions(polysaccharide dosage,adsorption time,pH and treating temperature)on Pb~(2+) and Cd~(2+ )adsorption by crude polysaccharide were also investigated.The highest yield of polysaccharide was 25.1% under the following condition:solid-liquid ratio of 40∶ 1 mL/g,temperature of 80 ℃ and extraction time of 5 h.Moreover,with the dosage of 100 mg,biosorption time of 3 h,pH 7.0,treating at 25 ℃,the equilibrium adsorption of Pb~(2+) and Cd~(2+) was up to 73.81 and 49.69 mg/g,respectively.Compared with Freundlich isotherm model,Langmuir isotherm model was more suitable to describe the adsorption of Pb~(2+) and Cd~(2+) by extracted polysaccharide.Depending on this study,Cyanobacteria collected from Taihu Lake could be the extraction source of polysaccharide,with the potential for absorption of heavy metals in polluted water.
Polysaccharide was extracted from Cyanobacteria collected from Taihu Lake,and further used to adsorb Pb~(2+) and Cd~(2+).Polysaccharide from Cyanobacteria was extracted with ultrasonic assisted hot water extraction,and extraction conditions,including extraction time,temperature and solid-liquid ratio,were optimized.Meanwhile,effects of adsorption conditions(polysaccharide dosage,adsorption time,pH and treating temperature)on Pb~(2+) and Cd~(2+ )adsorption by crude polysaccharide were also investigated.The highest yield of polysaccharide was 25.1% under the following condition:solid-liquid ratio of 40∶ 1 mL/g,temperature of 80 ℃ and extraction time of 5 h.Moreover,with the dosage of 100 mg,biosorption time of 3 h,pH 7.0,treating at 25 ℃,the equilibrium adsorption of Pb~(2+) and Cd~(2+) was up to 73.81 and 49.69 mg/g,respectively.Compared with Freundlich isotherm model,Langmuir isotherm model was more suitable to describe the adsorption of Pb~(2+) and Cd~(2+) by extracted polysaccharide.Depending on this study,Cyanobacteria collected from Taihu Lake could be the extraction source of polysaccharide,with the potential for absorption of heavy metals in polluted water.
引文
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[2] LIU L,QIN B,ZHANG Y,et al.Extraction and characterization of bound extracellular polymeric substances from cultured pure cyanobacterium (Microcystis wesenbergii)[J].J Environ Sci,2014,26(8):1725-1732.
[3] NOREEN A,ZIA K M,ZUBER M,et al.A critical review of algal biomass:a versatile platform of bio-based polyesters from renewable resources[J].Int J Biol Macromol,2016,86:937-949.
[4] 杨海麟,李克朗,张玲,等.蓝藻资源无害化利用技术的研究[J].生物技术,2008,18(6):95-97.
[5] 吴蕾,庞广昌,陈庆森.螺旋藻藻蓝蛋白的规模化提取和色谱纯化技术研究进展[J].食品科学,2008,29(4):461-463.
[6] 黄依佳,吴剑荣,朱莉,等.蓝藻多糖的分离、结构表征及抗氧化活性研究[J].食品与机械,2018,4(2):176-180.
[7] 张晓霜,王妙妙,辛萌,等.褐藻多糖抗病毒作用研究进展[J].中国海洋药物,2016(2):87-94.
[8] 邵海艳,吉宏武.总状蕨藻多糖体外抗肿瘤作用[J].食品科学,2014,35(11):251-254.
[9] ATHUKORALA Y,AHN G N,JEE Y H,et al.Antiproliferative activity of sulfated polysaccharide isolated from an enzymatic digest of Ecklonia cava on the U-937 cell line[J].J Appl Phycol,2009,21(3):307-314.
[10] AI L,ZHANG H,GUO B,et al.Preparation,partial characterization and bioactivity of exopolysaccharides from Lactobacillus casei LC2W[J].Carbohydr Polym,2008,74(3):353-357.
[11] 韩士群,严少华,王震宇,等.太湖蓝藻无害化处理资源化利用[J].自然资源学报,2009,24(3):431-438.
[12] 方璐希,喻鹏,刘灿明,等.铝钛改性MCM-41材料对污水中铅镉的吸附研究[J].环境科学与技术,2012,35(5):142-146.
[13] 邓景衡,余侃萍,肖国光,等.吸附法处理重金属废水研究进展[J].工业水处理,2014,34(11):4-7.
[14] LIU R H,DENG S,CHEN J W,et al.Method of modified corncob adsorbing heavy metal ions from water[J].Adv Mater Res,2014,955-959:2458-2462.
[15] SARKAR P,BOSNEAGA E,AUER M.Plant cell walls throughout evolution:towards a molecular understanding of their design principles[J].J Exp Bot,2010,60(13):3615-3635.
[16] ACOSTA M P,VALDMAN E,LEITE S G F,et al.Biosorption of copper by Paenibacillus polymyxacells and their exopolysaccharide[J].World J Microbiol Biotechnol,2005,21(6/7):1157-1163.
[17] 贡小清,虞锐鹏.微波消解-原子吸收光谱法测定蚕蛹中的微量元素[J].分析试验室,2008,27(10):66-69.
[18] YE S,ZHANG M,YANG H,et al.Biosorption of Cu2+,Pb2+ and Cr6+ by a novel exopolysaccharide from Arthrobacter ps-5[J].Carbohydr Polym,2014,101(1):50-56.
[19] KRZES?OWSKA M.The cell wall in plant cell response to trace metals:polysaccharide remodeling and its role in defense strategy[J].Acta Physiol Plant,2011,33(1):35-51.
[20] FENG M,CHEN X,LI C,et al.Isolation and identification of an exopolysaccharide-producing lactic acid bacterium strain from Chinese Paocai and biosorption of Pb(II) by its exopolysaccharide[J].J Food Sci,2012,77(6):111-117.
[21] 刘言炜,张发宇,汪家权.响应面法优化超声波辅助冻融提取蓝藻多糖工艺研究[J].食品科技,2018(5):198-205.
[22] YIN Y,HU Y,XIONG F.Sorption of Cu(II) and Cd(II) by extracellular polymeric substances (EPS) from Aspergillus fumigatus[J].Int Biodeter Biodegrad,2011,65(7):1012-1018.
[23] ONDITI M,ADELODUN A A,CHANGAMU E O,et al.Removal of Pb2+ and Cd2+ from drinking water using polysaccharide extract isolated from cactus pads (Opuntia ficus indica)[J].J Appl Polym Sci,2016,133(38):43913.
[24] 张蕊,葛滢.稻壳基活性炭制备及其对重金属吸附研究[J].环境污染与防治,2011,33(1):41-45.
[25] WANG J,CHEN C.Chitosan-based biosorbents:modification and application for biosorption of heavy metals and radionuclides[J].Bioresour Technol,2014,160(5):129-141.
[26] 王晓波,车海萍,陈海珍,等.榴莲壳内皮果胶多糖和黄酮对重金属吸附作用的研究[J].食品工业科技,2011(12):129-131.
[27] 杨婷,王洪良,吕春欣,等.改性硅藻土对重金属离子的吸附规律[J].吉林大学学报(理学版),2012,50(4):815-821.
[28] ZHEN P G,ZHI F Y,TIAN L Y,et al.Adsorption isotherm,thermodynamics and kinetics studies of polyphenols separation from kiwifruit juice using adsorbent resin[J].J Food Eng,2013,116(1):195-201.
[29] ROSS K,SIOW Y,DAN B,et al.Characterization of water extractable crude polysaccharides from cherry,raspberry,and ginseng berry fruits:chemical composition and bioactivity[J].Int J Food Properties,2015,18(3):670-689.