烟草根系分泌物对黑胫病菌菌丝生长的影响
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摘要
为了探讨烟草品种对黑胫病的抗性机制,以抗黑胫病品种Florida 301、L8、Beinhart1000-1和革新3号,以及感黑胫病品种中烟100、贵烟13、红花大金元和小黄金1025的盆栽烟苗为试验材料,采用生长速率法测定了抗感黑胫病烟草品种根系分泌物对黑胫病菌菌丝生长的影响。结果表明,抗病品种Florida301、L8、Beinhart1000-1和革新3号的根系分泌物对黑胫病菌菌丝生长具有较明显抑制作用,抑制率为5.93%～20.78%;4个抗病品种根系分泌物处理的菌落直径明显小于对照,且差异达到显著水平(P<0.05)。感病品种贵烟13、红花大金元和小黄金1025根系分泌物处理与对照相比对黑胫病菌菌丝生长具有一定的促进作用,菌落直径大于对照,差异达到极显著水平(P<0.01),菌丝生长抑制率为-16.47%～-2.15%,但感病品种中烟100没有表现出对黑胫病菌菌丝生长的促进作用。因此,烟草根系分泌物对黑胫病菌菌丝生长的作用与烟草品种的抗性有关。
In order to investigate the resistance mechanism of tobacco varieties to black shank, resistant varieties(Florida 301, L8, Beinhart1000-1 and Gexin 3) and susceptible varieties(Zhongyan 100, Guiyan 13,Honghuadajinyuan and Xiaohuangjin 1025) were selected to assess the effects of root exudates from eight tobacco varieties on the mycelium growth of Phytophthora parasitica var. nicotianae(Breda de Hean) Tuker. The results showed that the root exudates from the four resistant varieties had clear inhibitory effects on the mycelium growth of P. parasitica var. nicotianae with inhibition ratios from 5.93% to 20.78%. The colony diameters treated by root exudates from the four resistant varieties were significantly smaller than the control(P<0.05). Comparing with the control, the root exudates from susceptible varieties(Guiyan13,Honghuadajinyuan and Xiaohuangjin 1025) promoted the mycelium growth of P. parasitica var. nicotianae with the inhibitory ratios from-16.47% to-2.15%. The colony diameters treated by root exudates from the three susceptible varieties were extremely significantly larger than the control(P<0.01). However, susceptible variety Zhongyan 100 did not promote the mycelium growth of P. parasitica var. nicotianae. Therefore, the effects of tobacco root exudates on the mycelium growth of P. parasitica var. nicotianae were correlated to the resistance of tobacco varieties.
In order to investigate the resistance mechanism of tobacco varieties to black shank, resistant varieties(Florida 301, L8, Beinhart1000-1 and Gexin 3) and susceptible varieties(Zhongyan 100, Guiyan 13,Honghuadajinyuan and Xiaohuangjin 1025) were selected to assess the effects of root exudates from eight tobacco varieties on the mycelium growth of Phytophthora parasitica var. nicotianae(Breda de Hean) Tuker. The results showed that the root exudates from the four resistant varieties had clear inhibitory effects on the mycelium growth of P. parasitica var. nicotianae with inhibition ratios from 5.93% to 20.78%. The colony diameters treated by root exudates from the four resistant varieties were significantly smaller than the control(P<0.05). Comparing with the control, the root exudates from susceptible varieties(Guiyan13,Honghuadajinyuan and Xiaohuangjin 1025) promoted the mycelium growth of P. parasitica var. nicotianae with the inhibitory ratios from-16.47% to-2.15%. The colony diameters treated by root exudates from the three susceptible varieties were extremely significantly larger than the control(P<0.01). However, susceptible variety Zhongyan 100 did not promote the mycelium growth of P. parasitica var. nicotianae. Therefore, the effects of tobacco root exudates on the mycelium growth of P. parasitica var. nicotianae were correlated to the resistance of tobacco varieties.
引文
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[7]韩雪,潘凯,吴凤芝.不同抗性黄瓜品种根系分泌物对枯萎病病原菌的影响[J].中国蔬菜,2006,1(5):13-15.HAN Xue,PAN Kai,WU Fengzhi.Effect of root exudates from cucumber cultivars on pathogen of Fusarium wilt[J].China Vegetables,2006,1(5):13-15.
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[9]田永强,张作刚,张正,等.根系分泌物对西瓜枯萎病菌的影响[J].山西农业大学学报(自然科学版),2008,28(4):436-438.TIAN Yongqiang,ZHANG Zuogang,ZHANG Zheng,et al.Effects of the root exudates of watermelons on Fusarium oxysporum[J].Journal of Shanxi Agricultural University(Natural Science),2008,28(4):436-438.
[10]汤东生,王斌,毛忠顺,等.大蒜根系分泌物对石榴枯萎病菌和枯草芽孢杆菌的作用[J].安徽农业科学,2011,39(9):5294-5296.TANG Dongsheng,WANG Bin,MAO Zhongshun,et al.Study on garlic root exudates influence to growth of pomegranate wilt pathogen(Ceratocystis fimbriata)and Bacillus subtilis[J].Journal of Anhui Agricultural Sciences,2011,39(9):5294-5296.
[11]连莲香.特殊生境真菌的分离、活性筛选和次级代谢产物研究[D].厦门:厦门大学,2014.LIAN Lianxiang.Isolation,biological activity and secondary metabolite investigations of fungi from special habitat[D].Xiamen:Xiamen University,2014.
[12]方中达.植病研究方法[M].3版.北京:中国农业出版社,1998:46-50.FANG Zhongda.Plant disease research methods[M].3rd ed.Beijing:China Agriculture Press,1998:46-50.
[13]邱文龙.不同品种烟草根系分泌物的组分分析与抗黑胫病的关系[D].泰安:山东农业大学,2014.QIU Wenlong.Researches on the relationship between root exudates of different flue-cured tobacco varieties and resistant of black shank disease(Phytophora parasitica var.nicotianae)[D].Tai’an:Shandong Agricultural University,2014.
[14]周宝利,尹玉玲,李云鹏,等.嫁接茄根系分泌物与抗黄萎病的关系及其组分分析[J].生态学报,2010,30(11):3073-3079.ZHOU Baoli,YIN Yuling,LI Yunpeng et al.Relationship between root exudates of grafted eggplants and Verticillium wilt resistance and their components identification[J].Acta Ecologica Sinica,2010,30(11):3073-3079.
[15]周艳丽,程智慧,孟焕文.大蒜根系分泌物对不同受体蔬菜的化感作用[J].应用生态学报,2007,18(1):81-86.ZHOU Yanli,CHENG Zhihui,MENG Huanwen.Allelopathy of garlic root exudates on different receiver vegetables[J].Chinese Journal of Applied Ecology,2007,18(1):81-86.
[16]吴玉萍,邓建华,文大荣,等.云南不同品种和产区烤烟烟碱含量的差异[J].烟草科技,2010(9):40-42,50.WU Yuping,DENG Jianhua,WEN Darong,et al.Nicotine contents in flue-cured tobacco of different varieties and growing areas in Yunnan[J].Tobacco Science&Technology,2010(9):40-42,50.
[17]Vives-Peris V,Molina L,Segura A,et al.Root exudates from citrus plants subjected to abiotic stress conditions have a positive effect on rhizobacteria[J].Journal of Plant Physiology,2018,228:208-217.
[18]王海波,时焦.烟草嫁接苗对黑胫病的抗性鉴定及抗性机理研究[D].北京:中国农科院研究生院,2016.WANG Haibo,SHI Jiao.Resistance identification and resistant mechanism of tobacco-grafted seedlings to black shank[D].Beijing:Graduate School of Chinese Academy of Agricultural Sciences,2016.
[19]张庆平,刘中兴.荞麦根系分泌物对小麦全蚀病菌的抑制及根际微生物种群数量观察[J].内蒙古农业科技,1994(1):8-9.ZHANG Qingping,LIU Zhongxing.Inhibition of buckwheat root exudates on Gaeumannomyces graminis var.graminis(Sacc.)Walker and observation of rhizosphere microorganism population[J].Inner Mongolia Agricultural Science and Technology,1994(1):8-9.
[20]史刚荣.植物根系分泌物的生态效应[J].生态学杂志,2004,23(1):97-101.SHI Gangrong.Ecological effects of plant root exudates[J].Chinese Journal of Ecology,2004,23(1):97-101.
[21]黄奔立,许云东,伍烨,等.两个不同抗性黄瓜品种和云南黑籽南瓜根系分泌物对黄瓜枯萎病发生的影响[J].应用生态学报,2007,18(3):559-563.HUANG Benli,XU Yundong,WU Ye,et al.Effects of root exudates from cucumber and squash on Fusarium wilt occurrence[J].Chinese Journal of Applied Ecology,2007,18(3):559-563.
[22]朱文杰,陈欣.入侵植物加拿大一枝黄花(Solidago canadensis L.)对土传病菌的化感作用[D].杭州:浙江大学,2011.ZHU Wenjie,CHEN Xin.Allelopathic effects of invasive plant Solidago canadensis L.on soil-borne pathogens[D].Hangzhou:Zhejiang University,2011.
[23]冯超,战徊旭,崔萌萌,等.烟草根系分泌物对烟草疫霉菌的抑制作用[J].烟草科技,2018,51(9):7-13.FENG Chao,ZHAN Huaixu,CUI Mengmeng,et al.Inhibition effects of tobacco root exudates on Phytophthora parasitica var.nicotianae[J].Tobacco Science&Technology,2018,51(9):7-13.
[2]王志愿,姜清治,霍沁建.烟草黑胫病的研究进展[J].中国农学通报,2010,26(21):250-255.WANG Zhiyuan,JIANG Qingzhi,HUO Qinjian.Progress of research on tobacco black shank[J].Chinese Agricultural Science Bulletin,2010,26(21):250-255.
[3]朱贤朝,王彦亭,王智发.中国烟草病害[M].北京:中国农业出版社,2001.ZHU Xianchao,WANG Yanting,WANG Zhifa.Tobacco diseases of China[M].Beijing:China Agriculture Press,2001.
[4]陈泽斌,夏振远,雷丽萍,等.烟草黑胫病拮抗内生细菌的分离、鉴定及防效测定[J].中国烟草学报,2011,17(6):94-99.CHEN Zebin,XIA Zhenyuan,LEI Liping,et al.Isolation,identification and control efficacy determination of an endophytic strain against Phytophthora parasitica var.nicotianae[J].Acta Tabacaria Sinica,2011,17(6):94-99.
[5]涂书新,孙锦荷,郭智芬,等.植物根系分泌物与根际营养关系评述[J].土壤与环境,2000,9(1):64-67.TU Shuxin,SUN Jinhe,GUO Zhifen,et al.On relationship between root exudates and plant nutrition in rhizosphere[J].Soil and Environmental Sciences,2000,9(1):64-67.
[6]袁虹霞,李洪连,王烨,等.棉花不同抗性品种根系分泌物分析及其对黄萎病菌的影响[J].植物病理学报,2002,32(2):127-131.YUAN Hongxia,LI Honglian,WANG Ye,et al.The root exudates of cotton cultivars with the different resistance and their effects on Verticillium dahlia[J].Acta Phytopathologica Sinica,2002,32(2):127-131.
[7]韩雪,潘凯,吴凤芝.不同抗性黄瓜品种根系分泌物对枯萎病病原菌的影响[J].中国蔬菜,2006,1(5):13-15.HAN Xue,PAN Kai,WU Fengzhi.Effect of root exudates from cucumber cultivars on pathogen of Fusarium wilt[J].China Vegetables,2006,1(5):13-15.
[8]Smiley R W,Cook R J.Relationship between take-all of wheat and rhizosphere pH in soils fertilized with ammonium vs.nitrate-nitrogen[J].Phytopathology,1973,63(7):882-890.
[9]田永强,张作刚,张正,等.根系分泌物对西瓜枯萎病菌的影响[J].山西农业大学学报(自然科学版),2008,28(4):436-438.TIAN Yongqiang,ZHANG Zuogang,ZHANG Zheng,et al.Effects of the root exudates of watermelons on Fusarium oxysporum[J].Journal of Shanxi Agricultural University(Natural Science),2008,28(4):436-438.
[10]汤东生,王斌,毛忠顺,等.大蒜根系分泌物对石榴枯萎病菌和枯草芽孢杆菌的作用[J].安徽农业科学,2011,39(9):5294-5296.TANG Dongsheng,WANG Bin,MAO Zhongshun,et al.Study on garlic root exudates influence to growth of pomegranate wilt pathogen(Ceratocystis fimbriata)and Bacillus subtilis[J].Journal of Anhui Agricultural Sciences,2011,39(9):5294-5296.
[11]连莲香.特殊生境真菌的分离、活性筛选和次级代谢产物研究[D].厦门:厦门大学,2014.LIAN Lianxiang.Isolation,biological activity and secondary metabolite investigations of fungi from special habitat[D].Xiamen:Xiamen University,2014.
[12]方中达.植病研究方法[M].3版.北京:中国农业出版社,1998:46-50.FANG Zhongda.Plant disease research methods[M].3rd ed.Beijing:China Agriculture Press,1998:46-50.
[13]邱文龙.不同品种烟草根系分泌物的组分分析与抗黑胫病的关系[D].泰安:山东农业大学,2014.QIU Wenlong.Researches on the relationship between root exudates of different flue-cured tobacco varieties and resistant of black shank disease(Phytophora parasitica var.nicotianae)[D].Tai’an:Shandong Agricultural University,2014.
[14]周宝利,尹玉玲,李云鹏,等.嫁接茄根系分泌物与抗黄萎病的关系及其组分分析[J].生态学报,2010,30(11):3073-3079.ZHOU Baoli,YIN Yuling,LI Yunpeng et al.Relationship between root exudates of grafted eggplants and Verticillium wilt resistance and their components identification[J].Acta Ecologica Sinica,2010,30(11):3073-3079.
[15]周艳丽,程智慧,孟焕文.大蒜根系分泌物对不同受体蔬菜的化感作用[J].应用生态学报,2007,18(1):81-86.ZHOU Yanli,CHENG Zhihui,MENG Huanwen.Allelopathy of garlic root exudates on different receiver vegetables[J].Chinese Journal of Applied Ecology,2007,18(1):81-86.
[16]吴玉萍,邓建华,文大荣,等.云南不同品种和产区烤烟烟碱含量的差异[J].烟草科技,2010(9):40-42,50.WU Yuping,DENG Jianhua,WEN Darong,et al.Nicotine contents in flue-cured tobacco of different varieties and growing areas in Yunnan[J].Tobacco Science&Technology,2010(9):40-42,50.
[17]Vives-Peris V,Molina L,Segura A,et al.Root exudates from citrus plants subjected to abiotic stress conditions have a positive effect on rhizobacteria[J].Journal of Plant Physiology,2018,228:208-217.
[18]王海波,时焦.烟草嫁接苗对黑胫病的抗性鉴定及抗性机理研究[D].北京:中国农科院研究生院,2016.WANG Haibo,SHI Jiao.Resistance identification and resistant mechanism of tobacco-grafted seedlings to black shank[D].Beijing:Graduate School of Chinese Academy of Agricultural Sciences,2016.
[19]张庆平,刘中兴.荞麦根系分泌物对小麦全蚀病菌的抑制及根际微生物种群数量观察[J].内蒙古农业科技,1994(1):8-9.ZHANG Qingping,LIU Zhongxing.Inhibition of buckwheat root exudates on Gaeumannomyces graminis var.graminis(Sacc.)Walker and observation of rhizosphere microorganism population[J].Inner Mongolia Agricultural Science and Technology,1994(1):8-9.
[20]史刚荣.植物根系分泌物的生态效应[J].生态学杂志,2004,23(1):97-101.SHI Gangrong.Ecological effects of plant root exudates[J].Chinese Journal of Ecology,2004,23(1):97-101.
[21]黄奔立,许云东,伍烨,等.两个不同抗性黄瓜品种和云南黑籽南瓜根系分泌物对黄瓜枯萎病发生的影响[J].应用生态学报,2007,18(3):559-563.HUANG Benli,XU Yundong,WU Ye,et al.Effects of root exudates from cucumber and squash on Fusarium wilt occurrence[J].Chinese Journal of Applied Ecology,2007,18(3):559-563.
[22]朱文杰,陈欣.入侵植物加拿大一枝黄花(Solidago canadensis L.)对土传病菌的化感作用[D].杭州:浙江大学,2011.ZHU Wenjie,CHEN Xin.Allelopathic effects of invasive plant Solidago canadensis L.on soil-borne pathogens[D].Hangzhou:Zhejiang University,2011.
[23]冯超,战徊旭,崔萌萌,等.烟草根系分泌物对烟草疫霉菌的抑制作用[J].烟草科技,2018,51(9):7-13.FENG Chao,ZHAN Huaixu,CUI Mengmeng,et al.Inhibition effects of tobacco root exudates on Phytophthora parasitica var.nicotianae[J].Tobacco Science&Technology,2018,51(9):7-13.