黄芪灵芝口服液对免疫新城疫疫苗雏鸡免疫功能的影响
|
摘要
为研究黄芪灵芝口服液对雏鸡免疫功能的影响,本试验将180只14日龄雏鸡随机分成9组并通过点眼滴鼻免疫新城疫(ND)疫苗。1~3组分别是黄芪灵芝口服液高、中和低剂量组;4~6组分别是环磷酰胺+黄芪灵芝口服液高、中和低剂量组;7~9组分别为环磷酰胺组、阳性对照组及生理盐水对照组。分别于免疫后的第7、14、21、28天测定脾脏淋巴细胞增殖和抗体滴度的动态变化。结果表明,黄芪灵芝口服液能提高雏鸡脾脏淋巴细胞的增殖能力和促进新城疫病毒(NDV)抗体的产生,能一定程度上缓解环磷酰胺的免疫抑制,其中黄芪灵芝口服液低剂量组对脾脏淋巴细胞增殖效果最好,黄芪灵芝口服液可作为一种新型的免疫增强剂。
In order to study the effects of the oral liquid of Astragalus and Lucidum polysaccharides on immunological function in chickens,one hundred and eighty 14-day-old chickens were randomly divided into 9groups and inoculated with the Newcastle disease vaccine.1to 3experimental groups were respectively the oral liquid of Astragalus and Lucidumpolysaccharides high,middle and low dose groups;4to 6experimental groups were respectively the oral liquid of Astragalus and Lucidumpolysaccharides with cyclophosphamide high,middle and low dose groups;7to 9experimental groups were respectively cyclophosphamide group,positive control group and saline control group.The proliferation index of spleen lymphocyte and Newcastle disease virus antibody level by HI method were determined on days 7,14,21 and 28after vaccination.The results showed that the oral liquid of Astragalus and Lucidumpolysaccharides could apparently promote lymphocyte proliferation and enhance antibody titer,and alleviate the immunosupperession of the chickens in some degree,the low dose of the oral liquid of Astragalus and Lucidum polysaccharides promoted lymphocyte proliferation best,implying that the oral liquid of Astragalus and Lucidumpolysaccharides would be expected as a candidate of new-type immunopotentiator.
In order to study the effects of the oral liquid of Astragalus and Lucidum polysaccharides on immunological function in chickens,one hundred and eighty 14-day-old chickens were randomly divided into 9groups and inoculated with the Newcastle disease vaccine.1to 3experimental groups were respectively the oral liquid of Astragalus and Lucidumpolysaccharides high,middle and low dose groups;4to 6experimental groups were respectively the oral liquid of Astragalus and Lucidumpolysaccharides with cyclophosphamide high,middle and low dose groups;7to 9experimental groups were respectively cyclophosphamide group,positive control group and saline control group.The proliferation index of spleen lymphocyte and Newcastle disease virus antibody level by HI method were determined on days 7,14,21 and 28after vaccination.The results showed that the oral liquid of Astragalus and Lucidumpolysaccharides could apparently promote lymphocyte proliferation and enhance antibody titer,and alleviate the immunosupperession of the chickens in some degree,the low dose of the oral liquid of Astragalus and Lucidum polysaccharides promoted lymphocyte proliferation best,implying that the oral liquid of Astragalus and Lucidumpolysaccharides would be expected as a candidate of new-type immunopotentiator.
引文
[1]Xia M,Guo Z H,Wang D Y,et al.Effects of sulfated polysaccharides and their prescriptions on immune response of ND vaccine in chicken[J].Carbohydrate Polymers,2010,82:9-13.
[2]Huang X Y,Hu Y L,Zhao X N,et al.Sulfated modification the adjuvant activity of Astragalus polysaccharide for ND vaccine[J].Carbohydrate Polymers,2007,73:303-308.
[3]张斌.人工合成胸腺五肽对雏鸡免疫功能的影响[D].扬州:扬州大学,2007.
[4]高艳艳.黄芪多糖增强疫苗免疫效力及机理研究[D].北京:中国农业科学院,2006.
[5]Kong X F,Hu Y L,Zhao X N,et al.Effects of Chinese herbal medicinal ingredients on peripheral lymphocyte proliferation and serum antibody titer after vaccination in chicken[J].International Immunopharmacology,2004,4:975-982.
[6]Talarico L,Pujol C,Zibetti R,et al.The antiviral activity of sulfated polysaccharides against dengue virus is dependent on virus serotype and host cell[J].Antiviral Research,2005,66:103-110.
[7]杨发龙,岳华,罗薇,等.MTT法检测鸡外周学淋巴细胞增殖反应的最佳条件探讨[J].西南民族学院学报,2002,28(2):214-215.
[8]郝树彬,温红玲,尹玉霞,等.BALB/c鼠脾脏T淋巴细胞体外转化实验条件的优化研究[J].中国医疗器械信息,2011,17(8):31-34.
[9]何玉琴,杨小燕,黄其春,等.鸡脾脏T淋巴细胞增殖试验优化条件的筛选[J].动物医学进展,2008,29(7):47-49.
[10]Thiagarajan D,Ram G C,Bansal M P.Optimum conditions for in vitro chicken IL-2production and its in vivoin Newcastle disease vaccinated chickens[J].Veterinary Immunology and Immunopathology,1999,67:79-91.
[11]邓小红.中药免疫增强剂研究进展[J].重庆工商大学学报,2006,23(6):556-558.
[12]Yin G J,Thompson A,Adams Z,et al.Chinese herbs enhance immune response of carp,Cyprinus carpio,and protection against Aeromonas hydrophila[J].Fish and Shellfish Immunology,2009,26:140-145.
[13]郭小清,唐莉苹,杜海.黄芪多糖的药理作用及其在畜禽保健中的应用[J].中国动物保健,2012,3:45-47.
[14]Hou G,Cao R,Xu X.Study of Ganoderma lucidum polysaccharides(GLP)on the antitumor activities and immunoregulation mechanisms[J].Saengyak Hakhoechi,1995,26(2):187-192.
[15]高微微,曹丽,李展,等.黄芪成分对鸡外周血淋巴细胞增殖反应的影响[J].中国兽药杂志,2011,36(6):16-18.
[16]项杰,王育斌,徐涛,等.黄芪多糖在宿主抵抗李斯特菌中的作用[J].武汉大学学报(医学版),2007,28(6):741-743.
[17]刘端勇,赵海梅,周枫,等.黄芪多糖调节小鼠小肠黏膜淋巴细胞因子的表达[J].中国中医基础医学杂志,2008,14(9):692-693.
[18]张钰.灵芝多糖的硫酸化修饰及其衍生物抗肿瘤活性的初步研究[D].无锡:江南大学,2005.
[19]李奇,赵奎君.中草药免疫增强剂的研究概况[J].中国药房,2012,23(39):3737-3740.
[20]石达友,刘汉儒,黎健华,等.中药复方散剂对肉鸡生长及鸡新城疫灭火油苗免疫效果的影响[J].中兽医医药杂志,2004,3:14.
[21]John R U,Mark C,Thi T D,et al.Stimulation of tetanus toxoid-specific immune responses by a traditional Chinese herbal medicine[J].Vaccine,2009,27(47):6634.
[22]Wang C,Liu J,Ba Y,et al.Selection of Chinese herbal medicine immunologic adjuvant[J].Chinese Journal Veterinary Science and Tecnology,1999,1:32-33.
[23]郑铁锁.复方中药的增强免疫作用和对雏鸡的安全性测定[D].南京:南京农业大学,2009.
[24]张自广.中药复方多糖的优化提取及对小鼠免疫功能的影响[D].合肥:安徽农业大学,2011.
[25]孔祥峰.9种中药成分对新城疫Ⅳ系疫苗免疫雏鸡血清中血凝抑制抗体水平的影响[J].畜牧兽医学报,2004,35(4):468-472.
[2]Huang X Y,Hu Y L,Zhao X N,et al.Sulfated modification the adjuvant activity of Astragalus polysaccharide for ND vaccine[J].Carbohydrate Polymers,2007,73:303-308.
[3]张斌.人工合成胸腺五肽对雏鸡免疫功能的影响[D].扬州:扬州大学,2007.
[4]高艳艳.黄芪多糖增强疫苗免疫效力及机理研究[D].北京:中国农业科学院,2006.
[5]Kong X F,Hu Y L,Zhao X N,et al.Effects of Chinese herbal medicinal ingredients on peripheral lymphocyte proliferation and serum antibody titer after vaccination in chicken[J].International Immunopharmacology,2004,4:975-982.
[6]Talarico L,Pujol C,Zibetti R,et al.The antiviral activity of sulfated polysaccharides against dengue virus is dependent on virus serotype and host cell[J].Antiviral Research,2005,66:103-110.
[7]杨发龙,岳华,罗薇,等.MTT法检测鸡外周学淋巴细胞增殖反应的最佳条件探讨[J].西南民族学院学报,2002,28(2):214-215.
[8]郝树彬,温红玲,尹玉霞,等.BALB/c鼠脾脏T淋巴细胞体外转化实验条件的优化研究[J].中国医疗器械信息,2011,17(8):31-34.
[9]何玉琴,杨小燕,黄其春,等.鸡脾脏T淋巴细胞增殖试验优化条件的筛选[J].动物医学进展,2008,29(7):47-49.
[10]Thiagarajan D,Ram G C,Bansal M P.Optimum conditions for in vitro chicken IL-2production and its in vivoin Newcastle disease vaccinated chickens[J].Veterinary Immunology and Immunopathology,1999,67:79-91.
[11]邓小红.中药免疫增强剂研究进展[J].重庆工商大学学报,2006,23(6):556-558.
[12]Yin G J,Thompson A,Adams Z,et al.Chinese herbs enhance immune response of carp,Cyprinus carpio,and protection against Aeromonas hydrophila[J].Fish and Shellfish Immunology,2009,26:140-145.
[13]郭小清,唐莉苹,杜海.黄芪多糖的药理作用及其在畜禽保健中的应用[J].中国动物保健,2012,3:45-47.
[14]Hou G,Cao R,Xu X.Study of Ganoderma lucidum polysaccharides(GLP)on the antitumor activities and immunoregulation mechanisms[J].Saengyak Hakhoechi,1995,26(2):187-192.
[15]高微微,曹丽,李展,等.黄芪成分对鸡外周血淋巴细胞增殖反应的影响[J].中国兽药杂志,2011,36(6):16-18.
[16]项杰,王育斌,徐涛,等.黄芪多糖在宿主抵抗李斯特菌中的作用[J].武汉大学学报(医学版),2007,28(6):741-743.
[17]刘端勇,赵海梅,周枫,等.黄芪多糖调节小鼠小肠黏膜淋巴细胞因子的表达[J].中国中医基础医学杂志,2008,14(9):692-693.
[18]张钰.灵芝多糖的硫酸化修饰及其衍生物抗肿瘤活性的初步研究[D].无锡:江南大学,2005.
[19]李奇,赵奎君.中草药免疫增强剂的研究概况[J].中国药房,2012,23(39):3737-3740.
[20]石达友,刘汉儒,黎健华,等.中药复方散剂对肉鸡生长及鸡新城疫灭火油苗免疫效果的影响[J].中兽医医药杂志,2004,3:14.
[21]John R U,Mark C,Thi T D,et al.Stimulation of tetanus toxoid-specific immune responses by a traditional Chinese herbal medicine[J].Vaccine,2009,27(47):6634.
[22]Wang C,Liu J,Ba Y,et al.Selection of Chinese herbal medicine immunologic adjuvant[J].Chinese Journal Veterinary Science and Tecnology,1999,1:32-33.
[23]郑铁锁.复方中药的增强免疫作用和对雏鸡的安全性测定[D].南京:南京农业大学,2009.
[24]张自广.中药复方多糖的优化提取及对小鼠免疫功能的影响[D].合肥:安徽农业大学,2011.
[25]孔祥峰.9种中药成分对新城疫Ⅳ系疫苗免疫雏鸡血清中血凝抑制抗体水平的影响[J].畜牧兽医学报,2004,35(4):468-472.