玫瑰精油微乳制备及性质研究
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摘要
为改善玫瑰精油的水溶性和稳定性,采用转相乳化法制备玫瑰精油微乳,优化微乳制备工艺,优化静态顶空法测定微乳包封率,并对储藏稳定性进行研究。结果表明:玫瑰精油HLB值≥15.5,微乳的最佳制备工艺为:吐温80为表面活性剂,乙醇为助表面活性剂,最佳Km值为2,S/O值为9,选用磁力搅拌法,最佳制备温度为40℃,当水分质量分数为90%时,平均粒径为10.43 nm,PDI为0.07,包封率达89%。制备的可稀释微乳粒径稳定,溶液澄清透亮,储存稳定性好。
In order to improve the water solubility and stability of rose essential oil,the rose microemulsion was prepared by phase inversion emulsification. The microemulsion preparation process was optimized,the static headspace method had been optimised for obtaining the encapsulation efficiency of microemulsion,and the storage stability was also studied. The results showed that,the HLB value of rose essential oil was above 15.5,Tween 80 was choosed as the surfactant,ethanol was choosed as the cosurfactant,Km=2,S/O=9,magnetic stir was selected as the preparation method,40 ℃ was the suitable temperature. When 90 percent of water was present in the microemulsion,the average particle size was 10.43 nm,PDI was 0.07 and encapsulation efficiency reached 89%,The microemulsion was clear and bright,and had good stability.
In order to improve the water solubility and stability of rose essential oil,the rose microemulsion was prepared by phase inversion emulsification. The microemulsion preparation process was optimized,the static headspace method had been optimised for obtaining the encapsulation efficiency of microemulsion,and the storage stability was also studied. The results showed that,the HLB value of rose essential oil was above 15.5,Tween 80 was choosed as the surfactant,ethanol was choosed as the cosurfactant,Km=2,S/O=9,magnetic stir was selected as the preparation method,40 ℃ was the suitable temperature. When 90 percent of water was present in the microemulsion,the average particle size was 10.43 nm,PDI was 0.07 and encapsulation efficiency reached 89%,The microemulsion was clear and bright,and had good stability.
引文
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[18] PAN Guoliang,JIA Xiaobin,WEi Huihua,et al. Comparison among several preparation methods for pseudo-ternary phase diagrams of pharmaceutical microemulsions[J]. China Pharmacy Zhongguo Yaofang,2006,17(1):21-23.(in Chinese)
[19] SHEVACHMAN M,SHANI A,GARTI N. Formation and investigation of microemulsions based on jojoba oil and nonionic surfactants[J]. Journal of the American Oil Chemists'Society,2004,81(12):1143-1152.
[2] CHENG Jie,XIE Jianchun,SUN Baoguo. Composition and aromatic characteristics of rose essential oil produced in China[J].China Food Additives,2007(5):66-70.(in Chinese)
[3]余峰.玫瑰精油的提取、分析与应用研究[D].南昌:南昌大学,2011.
[4] ARKEN Taxiiener,Muhbul,LIU Xiaofeng,et al. Experimental observation on toxicity rose oil elastic capsule[J]. Endemic Diseases Bulletin,2007,22(6):22-24.(in Chinese)
[5] ZHU Weihong,XU Shiying,JIANG Bo. Peparation of microencapsulated mint oil using starch octenyIsuccinate[J]. Journal of Food Science and Biotechnology,2006,25(2):60-65.(in Chinese)
[6]于梦.薄荷油微乳化包埋及应用研究[D].无锡:江南大学,2008.
[7] FENG Junli,WANG Zhengwu,YU Huixin,et al. Study on microemulsion of VE[J]. Food and Mach Inery,2006,22(5):36-39.(in Chinese)
[8] LAWRENCE M J,REES G D. Microemulsion-based media as novel drug delivery systems.[J]. Advanced Drug Delivery Reviews,2000,45(1):89-121.
[9] LIANG Rong,MA Jianguo,ZHONG Fang. Research progress of nanoemulsion delivery system in functional food[J]. Journal of Food Science and Biotechnology,2013,32(6):561-568.(in Chinese)
[10] MALCOLMSON C,SATRA C,KANTARIA S,et al. Effect of oil on the level of solubilization of testosterone propionate into nonionic oil-in-water microemulsions[J]. Journal of Pharmaceutical Sciences,1998,87(1):109-116.
[11] SHAH N H,CARVAJAL M T,PATEL C I,et al. Self-emulsifying drug delivery systems(SEDDS)with polyglycolyzed glycerides for improving in vitro dissolution and oral absorption of lipophilic drugs[J]. International Journal of Pharmaceutics,1994,106(1):15-23.
[12]杜红.中药微乳载药体系关键技术研究[D].北京:北京中医药大学,2008.
[13] VON CORSWANT C,THOREN P E. Solubilization of sparingly soluble active compounds in lecithin-based microemulsions:influence on phase behavior and microstructure[J]. Langmuir,1999,15(11):3710-3717.
[14] XU S X,LI Y C,LIU X,et al. In vitro and in vivo antifungal activity of a water-dilutable cassia oil microemulsion against Geotrichum citri-aurantii[J]. Journal of the Science of Food and Agriculture,2012,92(13):2668-2671.
[15]王海洋.维生素E微乳的制备及其性质研究[D].无锡:江南大学,2012.
[16] HUIBERS P D T,SHAH D O. Evidence for synergism in nonionic surfactant mixtures:enhancement of solubilization in water-in-oil microemulsions[J]. Langmuir,1997,13(21):5762-5765.
[17] SHAABAN H A,EDRIS A E. Factors affecting the phase behavior and antimicrobial activity of carvacrol microemulsions[J].Journal of Oleo Science,2015,64(4):393-404.
[18] PAN Guoliang,JIA Xiaobin,WEi Huihua,et al. Comparison among several preparation methods for pseudo-ternary phase diagrams of pharmaceutical microemulsions[J]. China Pharmacy Zhongguo Yaofang,2006,17(1):21-23.(in Chinese)
[19] SHEVACHMAN M,SHANI A,GARTI N. Formation and investigation of microemulsions based on jojoba oil and nonionic surfactants[J]. Journal of the American Oil Chemists'Society,2004,81(12):1143-1152.