外源糖对盐胁迫下小黑麦幼苗糖代谢的影响
|
摘要
外源糖可以缓解盐害,提高植物耐盐性。为探究外源糖对盐胁迫下小黑麦幼苗糖代谢的影响及其机理,以小黑麦品种东农8809为材料,通过施加0.5mmol·L~(-1)葡萄糖和0.5mmol·L~(-1)蔗糖,探讨了100mmol·L~(-1) NaCl胁迫下外源糖处理的小黑麦幼苗相对电导率、碳水化合物含量和糖代谢关键酶活性的变化。结果表明,在盐胁迫下,与未经外源糖处理(CK)相比,外源葡萄糖、蔗糖预处理的小黑麦幼苗叶片的相对电导率显著降低,可溶性糖含量及蔗糖合成酶、蔗糖磷酸合成酶、果糖-6-磷酸激酶和己糖激酶活性提高,尤其是己糖激酶活性显著高于其他处理,丙酮酸激酶活性降低。说明盐胁迫下,施加外源糖可以促进蔗糖和葡萄糖进入蔗糖合成途径和糖酵解途径,促进淀粉向可溶性糖转化,从而提高可溶性糖含量,降低渗透势,提高细胞吸水能力,以缓解盐胁迫对小黑麦的伤害程度。
Salt stress destroys the integrity of cells and inhibits the growth of plants.Exogenous sugar can alleviate salt stress and improve salt tolerance in plants.In order to know the effects and antimicrobial properties of exogenous sugar on sugar metabolism in triticale seedling under 100mmol·L~(-1)NaCl,Dongnong 8809 was used as experimental material and treated with 0.5mmol·L~(-1) glucose and0.5mmol·L~(-1) sucrose.The relative electrical conductivity,carbohydrate content and activities of key enzymes in glycometabolism were studied,and the impact of exogenous sugar on triticale under salt stress was analyzed.The results showed that under salt stress,exogenous glucose and sucrose pretreatment can significantly reduce relative electrical conductivity,improve the content of soluble sugar and the activities of key enzymes,including sucrose synthase,sucrose phosphate synthase,fructose-6-diphosphate kinase and hexokinase,in glycometabolism especially the activity of hexokinase exceeds others significantly.So that exogenous sugar can promote glucose and sucrose to go into the sugar metabolic pathways,promote the transformation of starch to soluble sugar,improve the content of soluble sugar,reduce osmotic potential,and improve the water absorbing capacity.Thus,exogenous sugar can acleviate the injury of salt stress to triticale.
Salt stress destroys the integrity of cells and inhibits the growth of plants.Exogenous sugar can alleviate salt stress and improve salt tolerance in plants.In order to know the effects and antimicrobial properties of exogenous sugar on sugar metabolism in triticale seedling under 100mmol·L~(-1)NaCl,Dongnong 8809 was used as experimental material and treated with 0.5mmol·L~(-1) glucose and0.5mmol·L~(-1) sucrose.The relative electrical conductivity,carbohydrate content and activities of key enzymes in glycometabolism were studied,and the impact of exogenous sugar on triticale under salt stress was analyzed.The results showed that under salt stress,exogenous glucose and sucrose pretreatment can significantly reduce relative electrical conductivity,improve the content of soluble sugar and the activities of key enzymes,including sucrose synthase,sucrose phosphate synthase,fructose-6-diphosphate kinase and hexokinase,in glycometabolism especially the activity of hexokinase exceeds others significantly.So that exogenous sugar can promote glucose and sucrose to go into the sugar metabolic pathways,promote the transformation of starch to soluble sugar,improve the content of soluble sugar,reduce osmotic potential,and improve the water absorbing capacity.Thus,exogenous sugar can acleviate the injury of salt stress to triticale.
引文
[1]ROLLAND F,BAENA-GONZALEZ E,SHEEN J.Sugar sensing and signaling in plants:conserved and novel mechanisms[J].Annual Review of Plant Biology,2006,57:675.
[2]刘良全,张水利,景小元,等.几种化学调控物质对盐胁迫下小麦幼苗生长及生理指标的调控作用[J].麦类作物学报,2010,30(1):73.LIU L Q,ZHANG S L,JING X Y,et al.Effect of seed soaking with four chemical regulators on root and seedling growth of different wheat genotypes under nacl stresses[J].Journal of Triticeae Crops,2010,30(1):73.
[3]丁顺华.外源海藻糖对小麦耐盐性的效应[D].济南:山东师范大学,2001:3-6.DING S H.Effect of exogenous trehalose on salt tolerance of wheat[D].Jinan:Shandong Normal University,2001:3-6.
[4]刘慧,王立群,黄占权.对小黑麦啤酒制麦工艺的探讨[J].黑龙江粮油科技,2000,21(1):28.LIU H,WANG L Q,HUANG Z Q.Discussion of technology of triticale beer[J].Heilongjiang Oil Technology,2000,21(1):28.
[5]KEUNEN E,PESHEV D,VANGRONSVELD J,et al.Plant sugars are crucial players in the oxidative challenge during abiotic stress:extending the traditional concept[J].Plant Cell Environment,2013,36:1242.
[6]BAENA-GONZALEZ E,SHEEN J.Convergent energy and stress signaling[J].Cell,2008,12(9):1360.
[7]LIU F,JENSEN C R,ANDERSEN M N.A review of drought adaptation in crop plants:changes in vegetative and reproductive physiology induced by ABA-based chemical signals[J].Australian Journal of Agricultural Research,2005,56:1245.
[8]PRAXEDES S C,DA MATTA F M,LOUREIRO M E.Effects of long-term soil drought on photosynthesis and carbonhyd rate in mature robusta coffee(Coffea canephora Pierre var.kouillou)leaves[J].Environmental and Experimental Botany,2006,56:263.
[9]王玉凤,王庆祥,商丽威.NaCl和Na2SO4胁迫对玉米幼苗渗透调节物质含量的影响[J].玉米科学,2007,15(5):69.WANG Y F,WANG Q X,SHANG L W.Effects of NaCl and Na2SO4stress on osmotic adjustment substances in maize seedlings[J].Journal of Maize Sciences,2007,15(5):69.
[10]阮成江,谢庆良.盐胁迫下沙棘的渗透调节效应[J].植物资源与环境学报,2002,11(2):45.RUAN CH J,XIE Q L.Osmotic adjustment effect of Hippophaer hamnoides L.under salt stress[J].Journal of Plant Resources and Environment,2002,11(2):45.
[11]赵莹.外源糖缓解玉米盐胁迫生理生化机制研究[D].大庆:黑龙江八一农垦大学,2014:4-5.ZHAO Y.Effects of exogenous sugar on the attenuation of salt stress and its physiological basis in maize seedling[D].Daqing:Heilongjiang Bayi Agricultural University,2014:4-5.
[12]闫素芳,于洋,葛青,等.外源蔗糖对小麦幼苗耐盐性的影响[J].中国生态农业学报,2012,20(2):225.YAN S F,YU Y,GE Q,et al.Effect of exogenous sucrose application on wheat seeding salt tolerance[J].Chinese Journal of Eco-Agriculture,2012,20(2):225.
[13]丁顺华,李艳艳,王宝山.外源海藻糖对小麦幼苗耐盐性的影响[J].西北植物学报,2005,25(3):513.DING S H,LI Y Y,WANG B S.Effect of exogenous trehalose on salt tolerance of wheat seedling[J].Acta Botany Boreal-Occident Sinica,2005,25(3):513.
[14]王玉萍,于丹,李成,等.壳聚糖对盐胁迫下小麦种子萌发及幼苗生理特性的影响[J].干旱地区农业研究,2016,34(1):180.WANG Y P,YU D,LI C,et al.Effect of chitosan on seed germination and seedling physiological characters of wheat under salt stress[J].Agricultural Research in the Arid Areas,2016,34(1):180.
[15]李冰.浒苔硫酸多糖对NaCl胁迫下植物的影响[D].北京:中国科学院大学,2013:2-3.LI B.Effect of sulfated polysaccharides from Enteromorpha prolifera on plants under NaCl stress[D].Beijing:Chinese Academy of Sciences,2013:2-3.
[16]郭伟,于立河.腐殖酸浸种对盐胁迫下小麦萌发种子及幼苗生理特性的影响[J].麦类作物学报,2012,32(1):90.GUO W,YU L H.Effect of seed soaking with humic acid on soluble sugar accumulation and allocation in germinated wheat seed under salt stress[J].Journal of Triticeae Crops,2012,32(1):90.
[17]LUTTS S,KINET J M,BOUHARMONT J.NaCl-induced senescence in leaves of rice(Oryza sativa L.)cultivars differing in salinity resistance[J].Annals of Botany,1996,78:389.
[18]刘丽杰.低温下ABA调控冬小麦糖代谢及抗寒基因表达的研究[D].哈尔滨:东北农业大学博士学位论文,2013:11-14.LIU L J.Effects of ABA on sugar metabolism and expression of antifreeze genes of winter wheat[D].Harbin:Northeast Agricultural University,2013:11-14.
[19]李改玲.外源糖调控盐胁迫下小黑麦糖代谢及光合特性的研究[D].哈尔滨:东北农业大学,2016.LI G L.Exogenous sugar alleviate effects of salt stress on the triticale’s sugar metabolism and photosynthetic characteristics[D].Harbin:Northeast Agricultural University,2016.
[20]DEMIRAL T,TRKAN I.Exogenous glycinebetaine affects growth and proline accumulation and retards senescence in two rice cultivars under NaCl stress[J].Journal Environmental and Experimental Botany,2006,56(1):72.
[21]凌腾芳,宣伟,樊颖瑞,等.外源葡萄糖、果糖和NO供体(SNP)对盐胁迫下水稻种子萌发的影响[J].植物生理与分子生物学学报,2005,31(2):205.LING T F,XUAN W,FAN Y R,et al.Effect of exogenous glucose and fructose and snp on rice seed germination under salt stress[J].Journal of Plant Physiolory and Molecular Biology,2005,31(2):205.
[22]杨涓.盐胁迫下枸杞糖蛋白及糖代谢变化的研究[D].银川:宁夏大学,2005:7-11.YANG J.Researches on cell wall glycoprotiein and sucrosemetabolizing of Lycium Barbarum L.under salt stress[D].Ningxia:Ningxia University,2005:7-11.
[23]KRISHNARA S,THORPE T A.Salinity stress effects on[14 C-1]-and[14 C-6]-glucose metabolism of a salt-tolerant and salt-susceptiblevariety of wheat[J].International Journal of Plant Science,1996,157(1):110.
[24]鲁少尉,齐飞,李天来.NaCl及等渗PEG胁迫对番茄叶片光合特性及蔗糖代谢的影响[J].华北农学报,2012,27(3):136.LU S W,QI F,LI T L.Effect of NaCl and PEG iso-osmotic stresses on photosynthetic characteristics and sucrose metabolizing in tomato leaf[J].Acta Agriculturae Boreali-Simica,2012,27(3):136.
[2]刘良全,张水利,景小元,等.几种化学调控物质对盐胁迫下小麦幼苗生长及生理指标的调控作用[J].麦类作物学报,2010,30(1):73.LIU L Q,ZHANG S L,JING X Y,et al.Effect of seed soaking with four chemical regulators on root and seedling growth of different wheat genotypes under nacl stresses[J].Journal of Triticeae Crops,2010,30(1):73.
[3]丁顺华.外源海藻糖对小麦耐盐性的效应[D].济南:山东师范大学,2001:3-6.DING S H.Effect of exogenous trehalose on salt tolerance of wheat[D].Jinan:Shandong Normal University,2001:3-6.
[4]刘慧,王立群,黄占权.对小黑麦啤酒制麦工艺的探讨[J].黑龙江粮油科技,2000,21(1):28.LIU H,WANG L Q,HUANG Z Q.Discussion of technology of triticale beer[J].Heilongjiang Oil Technology,2000,21(1):28.
[5]KEUNEN E,PESHEV D,VANGRONSVELD J,et al.Plant sugars are crucial players in the oxidative challenge during abiotic stress:extending the traditional concept[J].Plant Cell Environment,2013,36:1242.
[6]BAENA-GONZALEZ E,SHEEN J.Convergent energy and stress signaling[J].Cell,2008,12(9):1360.
[7]LIU F,JENSEN C R,ANDERSEN M N.A review of drought adaptation in crop plants:changes in vegetative and reproductive physiology induced by ABA-based chemical signals[J].Australian Journal of Agricultural Research,2005,56:1245.
[8]PRAXEDES S C,DA MATTA F M,LOUREIRO M E.Effects of long-term soil drought on photosynthesis and carbonhyd rate in mature robusta coffee(Coffea canephora Pierre var.kouillou)leaves[J].Environmental and Experimental Botany,2006,56:263.
[9]王玉凤,王庆祥,商丽威.NaCl和Na2SO4胁迫对玉米幼苗渗透调节物质含量的影响[J].玉米科学,2007,15(5):69.WANG Y F,WANG Q X,SHANG L W.Effects of NaCl and Na2SO4stress on osmotic adjustment substances in maize seedlings[J].Journal of Maize Sciences,2007,15(5):69.
[10]阮成江,谢庆良.盐胁迫下沙棘的渗透调节效应[J].植物资源与环境学报,2002,11(2):45.RUAN CH J,XIE Q L.Osmotic adjustment effect of Hippophaer hamnoides L.under salt stress[J].Journal of Plant Resources and Environment,2002,11(2):45.
[11]赵莹.外源糖缓解玉米盐胁迫生理生化机制研究[D].大庆:黑龙江八一农垦大学,2014:4-5.ZHAO Y.Effects of exogenous sugar on the attenuation of salt stress and its physiological basis in maize seedling[D].Daqing:Heilongjiang Bayi Agricultural University,2014:4-5.
[12]闫素芳,于洋,葛青,等.外源蔗糖对小麦幼苗耐盐性的影响[J].中国生态农业学报,2012,20(2):225.YAN S F,YU Y,GE Q,et al.Effect of exogenous sucrose application on wheat seeding salt tolerance[J].Chinese Journal of Eco-Agriculture,2012,20(2):225.
[13]丁顺华,李艳艳,王宝山.外源海藻糖对小麦幼苗耐盐性的影响[J].西北植物学报,2005,25(3):513.DING S H,LI Y Y,WANG B S.Effect of exogenous trehalose on salt tolerance of wheat seedling[J].Acta Botany Boreal-Occident Sinica,2005,25(3):513.
[14]王玉萍,于丹,李成,等.壳聚糖对盐胁迫下小麦种子萌发及幼苗生理特性的影响[J].干旱地区农业研究,2016,34(1):180.WANG Y P,YU D,LI C,et al.Effect of chitosan on seed germination and seedling physiological characters of wheat under salt stress[J].Agricultural Research in the Arid Areas,2016,34(1):180.
[15]李冰.浒苔硫酸多糖对NaCl胁迫下植物的影响[D].北京:中国科学院大学,2013:2-3.LI B.Effect of sulfated polysaccharides from Enteromorpha prolifera on plants under NaCl stress[D].Beijing:Chinese Academy of Sciences,2013:2-3.
[16]郭伟,于立河.腐殖酸浸种对盐胁迫下小麦萌发种子及幼苗生理特性的影响[J].麦类作物学报,2012,32(1):90.GUO W,YU L H.Effect of seed soaking with humic acid on soluble sugar accumulation and allocation in germinated wheat seed under salt stress[J].Journal of Triticeae Crops,2012,32(1):90.
[17]LUTTS S,KINET J M,BOUHARMONT J.NaCl-induced senescence in leaves of rice(Oryza sativa L.)cultivars differing in salinity resistance[J].Annals of Botany,1996,78:389.
[18]刘丽杰.低温下ABA调控冬小麦糖代谢及抗寒基因表达的研究[D].哈尔滨:东北农业大学博士学位论文,2013:11-14.LIU L J.Effects of ABA on sugar metabolism and expression of antifreeze genes of winter wheat[D].Harbin:Northeast Agricultural University,2013:11-14.
[19]李改玲.外源糖调控盐胁迫下小黑麦糖代谢及光合特性的研究[D].哈尔滨:东北农业大学,2016.LI G L.Exogenous sugar alleviate effects of salt stress on the triticale’s sugar metabolism and photosynthetic characteristics[D].Harbin:Northeast Agricultural University,2016.
[20]DEMIRAL T,TRKAN I.Exogenous glycinebetaine affects growth and proline accumulation and retards senescence in two rice cultivars under NaCl stress[J].Journal Environmental and Experimental Botany,2006,56(1):72.
[21]凌腾芳,宣伟,樊颖瑞,等.外源葡萄糖、果糖和NO供体(SNP)对盐胁迫下水稻种子萌发的影响[J].植物生理与分子生物学学报,2005,31(2):205.LING T F,XUAN W,FAN Y R,et al.Effect of exogenous glucose and fructose and snp on rice seed germination under salt stress[J].Journal of Plant Physiolory and Molecular Biology,2005,31(2):205.
[22]杨涓.盐胁迫下枸杞糖蛋白及糖代谢变化的研究[D].银川:宁夏大学,2005:7-11.YANG J.Researches on cell wall glycoprotiein and sucrosemetabolizing of Lycium Barbarum L.under salt stress[D].Ningxia:Ningxia University,2005:7-11.
[23]KRISHNARA S,THORPE T A.Salinity stress effects on[14 C-1]-and[14 C-6]-glucose metabolism of a salt-tolerant and salt-susceptiblevariety of wheat[J].International Journal of Plant Science,1996,157(1):110.
[24]鲁少尉,齐飞,李天来.NaCl及等渗PEG胁迫对番茄叶片光合特性及蔗糖代谢的影响[J].华北农学报,2012,27(3):136.LU S W,QI F,LI T L.Effect of NaCl and PEG iso-osmotic stresses on photosynthetic characteristics and sucrose metabolizing in tomato leaf[J].Acta Agriculturae Boreali-Simica,2012,27(3):136.