低温胁迫下玉米幼苗根系受外源海藻糖调控的生理表现
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摘要
为了探究海藻糖对增强玉米幼苗抗低温能力的生理表现,以玉米"先玉335"为试验材料,采用盆栽试验,分析12 mmol·L-1外源海藻糖对低温胁迫不同时间(0、2、4、6和8d)下玉米幼苗根系生物量、抗氧化酶活性及渗透调节物质的影响。结果表明:低温胁迫显著抑制玉米幼苗根系生长及干物质累积,诱导抗氧化酶活性增加,破坏细胞渗透调节水平;低温胁迫后应用外源海藻糖,玉米幼苗根系根表面积、根长及鲜干重显著增加,根系内SOD、POD和CAT活性呈现先升高后降低趋势,MDA含量及相对电导率在玉米幼苗根系内显著降低,脯氨酸、可溶性蛋白、可溶性糖含量也呈现先升高后降低趋势;在低温胁迫第6天时,T+L处理的根系根表面积、根长及鲜干重分别较CK+L增加了18.25%、4.73%、2.48%和21.43%;T+L处理后根系内SOD、POD和CAT活性分别较CK+L处理提升17.26%、23.79%和30.78%;T+L处理后根系内MDA含量和相对电导率值分别较CK+L处理降低45.13%和63.25%;T+L处理后的脯氨酸、可溶性蛋白和可溶性糖含量在低温胁迫第6天时最高,与CK+L处理相比差异显著,且分别较CK+L高出2.93、0.51和2.58倍。可见,海藻糖能够调控低温胁迫下玉米幼苗根系生理水平,提高玉米幼苗抗逆能力,促进玉米根系生长及生物量增加,海藻糖对作物生长的生理调控能力与低温存在协同作用,在低温环境下,海藻糖对作物体内抗氧化系统酶活表达及渗透物质的积累有显著提升效果。
To investigate the physiological properties of maize seedlings in enhancing the chilling resistance under exogenous trehalose,a pot experiment was carried out with ‘Xianyu 335'. The effects of 12 mmol·L-1 exogenous trehalose on root biomass,antioxidant enzyme activities and osmotic adjustment substances of maize seedlings at different chilling temperatures(0,2,4,6 and 8 d) were examined. The results showed that chilling stress significantly inhibited root growth and dry matter accumulation of maize seedlings,increased antioxidant enzyme activity,and destroyed the level of osmotic regulation. In response to trehalose application,root surface area,root length and fresh dry weight of maize seedling significantly increased,activities of SOD,POD and CAT in roots first increased and then decreased,MDA content and relative conductivity in roots significantly reduced. The contents of proline,soluble protein and soluble sugar in the roots were also increased first and then decreased. Compared with the CK+L treatment at the 6 th day of chilling treatment,the root surface area,root length and dry weight under the treatment of T +L increased by 18.25%,4. 73%,2. 48% and 21. 43%,respectively. The activities of SOD,POD and CAT significantly increased by 17.26%,23.79% and 30.78%,respectively. The MDA content and relative conductivity significantly decreased by 45.13% and 63.25%,respectively. The contents of proline,soluble protein and soluble sugar in T+L treatment were the highest at the 6 th day after chilling stress,being 2. 93,0. 51 and 2. 58 times higher than that under CK + L treatment,respectively. It is concluded that trehalose can regulate the physiological level of roots of maize seedlings under chilling stress,improve the ability of stress resistance of maize seedlings,and accelerate root growth. Trehalose has a synergistic effect on the physiological regulation of crop growth under low temperature stress. At chilling environment,trehalose can significantly enhance the expression of enzymes in the antioxidant system and the accumulation of osmotic substances.
To investigate the physiological properties of maize seedlings in enhancing the chilling resistance under exogenous trehalose,a pot experiment was carried out with ‘Xianyu 335'. The effects of 12 mmol·L-1 exogenous trehalose on root biomass,antioxidant enzyme activities and osmotic adjustment substances of maize seedlings at different chilling temperatures(0,2,4,6 and 8 d) were examined. The results showed that chilling stress significantly inhibited root growth and dry matter accumulation of maize seedlings,increased antioxidant enzyme activity,and destroyed the level of osmotic regulation. In response to trehalose application,root surface area,root length and fresh dry weight of maize seedling significantly increased,activities of SOD,POD and CAT in roots first increased and then decreased,MDA content and relative conductivity in roots significantly reduced. The contents of proline,soluble protein and soluble sugar in the roots were also increased first and then decreased. Compared with the CK+L treatment at the 6 th day of chilling treatment,the root surface area,root length and dry weight under the treatment of T +L increased by 18.25%,4. 73%,2. 48% and 21. 43%,respectively. The activities of SOD,POD and CAT significantly increased by 17.26%,23.79% and 30.78%,respectively. The MDA content and relative conductivity significantly decreased by 45.13% and 63.25%,respectively. The contents of proline,soluble protein and soluble sugar in T+L treatment were the highest at the 6 th day after chilling stress,being 2. 93,0. 51 and 2. 58 times higher than that under CK + L treatment,respectively. It is concluded that trehalose can regulate the physiological level of roots of maize seedlings under chilling stress,improve the ability of stress resistance of maize seedlings,and accelerate root growth. Trehalose has a synergistic effect on the physiological regulation of crop growth under low temperature stress. At chilling environment,trehalose can significantly enhance the expression of enzymes in the antioxidant system and the accumulation of osmotic substances.
引文
曹宁,符力,张玉斌,等.2008.低温对玉米苗期根系生长及磷养分吸收的影响.玉米科学,16(4):58-60.
陈慧.2016.低温对玉米苗期生长发育的影响极其防治措施.吉林农业,(10):95-108.
陈小凤,黄如葵,黄玉辉,等.2017.低温胁迫下苦瓜苗期生理变化与耐冷性评价的关系分析.南方农业学报,48(7):1237-1241.
郝再彬.2004.植物生理实.哈尔滨:哈尔滨工业大学出版社.
胡慧芳,马有会.2008.外源海藻糖提高黄瓜抗旱性研究初探.沈阳农业大学学报,39(1):83-85.
胡慧芳.2008.外源海藻糖对黄瓜幼苗抗冷性的影响.北方园艺,(2):11-13.
姜辉.2016.苗期低温胁迫对玉米根系生长的影响.黑龙江农业科学,(2):15-16.
康云艳,杨暹,郭世荣,等.2011.24-表油菜素内酯对低氧胁迫下黄瓜幼苗碳水化合物代谢的影响.中国农业科学,44(12):2495-2503.
李晶,张丽芳,焦健,等.2015.低温胁迫下外源ABA对玉米幼苗生长的影响.东北农业大学学报,46(11):1-7.
李春燕,陈思思,徐雯,等.2011.苗期低温胁迫对扬麦16叶片抗氧化酶和渗透调节物质的影响.作物学报,37(12):2293-2298.
李合生.2000.植物生理生化试验指导.北京:高等教育出版社.
李丽杰,顾万荣,李丛锋,等.2016.DCPTA对低温下玉米叶片抗氧化系统及渗透调节物质的影响.植物生理学报,52(12):1829-1841.
刘姣,姜大刚.2014.作物海藻糖合成相关基因的研究进展.基因组学与应用生物学,33(2):432-437.
刘岩.2016.外源海藻糖对番茄幼苗生化指标的影响.中国园艺文摘,(12):59-95.
刘丰娇,蔡冰冰,孙胜楠,等.2017.富氢水浸种增强黄瓜幼苗耐冷性的作用及其生理机制.中国农业科学,50(5):881-889.
罗宁,魏,李晶,等.2014.低温胁迫对玉米苗期根系特征及电导率的影响.生态学杂志,33(10):2694-2699.
马光恕,廉华,靳亚忠,等.2013.海藻糖对Na Cl胁迫下甜瓜幼苗生长的影响.广东农业科学,(16):40-43.
邵怡若,许建新,薛立,等.2013.低温胁迫时间对4种幼苗生理生化及光合特性的影响.生态学报,33(14):4237-4247.
史红梅,张海燕,杨彬,等.2015.低温胁迫对高亮幼苗MDA含量、SOD和POD活性的影响.中国农学通报,31(18):74-79.
孙富,杨丽涛,谢晓娜,等.2012.低温胁迫对不同抗寒性甘蔗品种幼苗叶绿体生理代谢的影响.作物学报,38(4):732-739.
孙阳,王邁,曲丹阳,等.2016.5-氨基乙酰丙酸提高玉米幼苗抗低温性及其生理机制.生态学杂志,35(7):1737-1743.
田礼欣,毕文双,曲丹阳,等.2017.海藻糖对盐胁迫下玉米幼苗生长及生理特性的影响.草业学报,26(8):131-138.
王芳,李永生,王汉宁,等.2016.钙对铅胁迫下玉米幼苗生长及生理特性的影响.水土保持学报,30(3):202-207.
魏.2013.黑龙江玉米生态生理与栽培.北京:中国农业出版社.
谢冬微,王晓楠,付连双,等.2015.外源海藻糖对冬小麦低温下胚芽长及幼苗抗寒性的影响.麦类作物学报,35(2):215-223.
徐婷,周传余,周超,等.2014.海藻糖对盐胁迫下薄皮甜瓜幼苗抗氧化系统的影响.北方园艺,(19):28-30.
徐向丽,易克,蒋红梅,等.2010.外源海藻糖对干旱胁迫下烟草幼苗抗旱性的影响.安徽农业科学,38(33):18675-18677.
闫道良,郑炳松.2016.海藻糖浸种对盐胁迫下杨麦19生理特性的影响.浙江农业学报,28(8):1271-1276.
杨舒贻,陈晓阳,惠文凯,等.2016.逆境胁迫下植物抗氧化酶系统响应研究进展.福建农林大学学报:自然科学版,45(5):481-489.
张鄖,顾万荣,王永超,等.2015.DCPTA对盐胁迫下玉米苗期根系生长、渗透调节及膜透性的影响.生态学杂志,34(9):2474-2481.
张保青,杨丽涛,李杨瑞.2011.自然条件下甘蔗品种抗寒生理生化特性的比较.作物学报,37(3):496-505.
邹琦.2000.植物生理学实验指导.北京:中国农业出版社.
Ali Q,Ashraf M,Anwar F,et al.2012.Trehalose-induced changes in seed oil composition and antioxidant potential of maize grown under drought stress.Journal of the American Oil Chemists,Society,89:1485-1493.
Azymi S,Sofalian O,Jahanbakhsh G,et al.2012.Effect of chilling stress on soluble protein,sugar and prolin accumulation in cotton(Gossypium hirsutum L.)genotypes.International Journal of Agriculture and Crop Sciences,4:825-830.
Farooq M,Aziz T,Heema ZA,et al.2008.Activation of antioxidant system by KCl improves the chilling tolerance in hybrid maize.Journal of Agronomy and Crop Science,194:438-448.
Gill SS,Tuteja N.2010.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants.Plant Physiology and Biochemistry,48:909.
Lins RD,Pereira CS,Nenberger PH,et al.2010.Trehaloseprotein interaction in aqueous solution.Proteins:Structure,Function and Bioinformatics,55:177-186.
Matthew P.2007.Trehalose 6-phosphate.Current Opinion in Plant Biology,10:303-309.
Nathan C,Ding A,Nathan C,et al.2010.Snap Shot:Reactive oxygen intermediates(ROI).Cell,140:952.e1.
Pierret A,Doussan C,Capowiez Y,et al.2007.Root functional architecture:A framework for modeling the inter-play between roots and soil.Vadose Zone Journal,6:269-281.
Steinbrenner H,Sies H.2009.Protection against reactive oxygen species by selenoproteins.Biochimica et Biophysica Acta,1790:1478-1485.
Tarek EB,Haluk H,Hüseyin A,et al.2005.Biochemical analysis of trehalose and its metabolizing enzymes in wheat under abiotic stress conditions.Plant Science,169:47-54.
Theerakulpisut P,Gunnula W.2012.Exogenous sorbitol and trehalose mitigated salt stress damage in salt-sensitive but not salt-tolerant rice seedlings.Asian Journal of Crop Science,4:165-170.
陈慧.2016.低温对玉米苗期生长发育的影响极其防治措施.吉林农业,(10):95-108.
陈小凤,黄如葵,黄玉辉,等.2017.低温胁迫下苦瓜苗期生理变化与耐冷性评价的关系分析.南方农业学报,48(7):1237-1241.
郝再彬.2004.植物生理实.哈尔滨:哈尔滨工业大学出版社.
胡慧芳,马有会.2008.外源海藻糖提高黄瓜抗旱性研究初探.沈阳农业大学学报,39(1):83-85.
胡慧芳.2008.外源海藻糖对黄瓜幼苗抗冷性的影响.北方园艺,(2):11-13.
姜辉.2016.苗期低温胁迫对玉米根系生长的影响.黑龙江农业科学,(2):15-16.
康云艳,杨暹,郭世荣,等.2011.24-表油菜素内酯对低氧胁迫下黄瓜幼苗碳水化合物代谢的影响.中国农业科学,44(12):2495-2503.
李晶,张丽芳,焦健,等.2015.低温胁迫下外源ABA对玉米幼苗生长的影响.东北农业大学学报,46(11):1-7.
李春燕,陈思思,徐雯,等.2011.苗期低温胁迫对扬麦16叶片抗氧化酶和渗透调节物质的影响.作物学报,37(12):2293-2298.
李合生.2000.植物生理生化试验指导.北京:高等教育出版社.
李丽杰,顾万荣,李丛锋,等.2016.DCPTA对低温下玉米叶片抗氧化系统及渗透调节物质的影响.植物生理学报,52(12):1829-1841.
刘姣,姜大刚.2014.作物海藻糖合成相关基因的研究进展.基因组学与应用生物学,33(2):432-437.
刘岩.2016.外源海藻糖对番茄幼苗生化指标的影响.中国园艺文摘,(12):59-95.
刘丰娇,蔡冰冰,孙胜楠,等.2017.富氢水浸种增强黄瓜幼苗耐冷性的作用及其生理机制.中国农业科学,50(5):881-889.
罗宁,魏,李晶,等.2014.低温胁迫对玉米苗期根系特征及电导率的影响.生态学杂志,33(10):2694-2699.
马光恕,廉华,靳亚忠,等.2013.海藻糖对Na Cl胁迫下甜瓜幼苗生长的影响.广东农业科学,(16):40-43.
邵怡若,许建新,薛立,等.2013.低温胁迫时间对4种幼苗生理生化及光合特性的影响.生态学报,33(14):4237-4247.
史红梅,张海燕,杨彬,等.2015.低温胁迫对高亮幼苗MDA含量、SOD和POD活性的影响.中国农学通报,31(18):74-79.
孙富,杨丽涛,谢晓娜,等.2012.低温胁迫对不同抗寒性甘蔗品种幼苗叶绿体生理代谢的影响.作物学报,38(4):732-739.
孙阳,王邁,曲丹阳,等.2016.5-氨基乙酰丙酸提高玉米幼苗抗低温性及其生理机制.生态学杂志,35(7):1737-1743.
田礼欣,毕文双,曲丹阳,等.2017.海藻糖对盐胁迫下玉米幼苗生长及生理特性的影响.草业学报,26(8):131-138.
王芳,李永生,王汉宁,等.2016.钙对铅胁迫下玉米幼苗生长及生理特性的影响.水土保持学报,30(3):202-207.
魏.2013.黑龙江玉米生态生理与栽培.北京:中国农业出版社.
谢冬微,王晓楠,付连双,等.2015.外源海藻糖对冬小麦低温下胚芽长及幼苗抗寒性的影响.麦类作物学报,35(2):215-223.
徐婷,周传余,周超,等.2014.海藻糖对盐胁迫下薄皮甜瓜幼苗抗氧化系统的影响.北方园艺,(19):28-30.
徐向丽,易克,蒋红梅,等.2010.外源海藻糖对干旱胁迫下烟草幼苗抗旱性的影响.安徽农业科学,38(33):18675-18677.
闫道良,郑炳松.2016.海藻糖浸种对盐胁迫下杨麦19生理特性的影响.浙江农业学报,28(8):1271-1276.
杨舒贻,陈晓阳,惠文凯,等.2016.逆境胁迫下植物抗氧化酶系统响应研究进展.福建农林大学学报:自然科学版,45(5):481-489.
张鄖,顾万荣,王永超,等.2015.DCPTA对盐胁迫下玉米苗期根系生长、渗透调节及膜透性的影响.生态学杂志,34(9):2474-2481.
张保青,杨丽涛,李杨瑞.2011.自然条件下甘蔗品种抗寒生理生化特性的比较.作物学报,37(3):496-505.
邹琦.2000.植物生理学实验指导.北京:中国农业出版社.
Ali Q,Ashraf M,Anwar F,et al.2012.Trehalose-induced changes in seed oil composition and antioxidant potential of maize grown under drought stress.Journal of the American Oil Chemists,Society,89:1485-1493.
Azymi S,Sofalian O,Jahanbakhsh G,et al.2012.Effect of chilling stress on soluble protein,sugar and prolin accumulation in cotton(Gossypium hirsutum L.)genotypes.International Journal of Agriculture and Crop Sciences,4:825-830.
Farooq M,Aziz T,Heema ZA,et al.2008.Activation of antioxidant system by KCl improves the chilling tolerance in hybrid maize.Journal of Agronomy and Crop Science,194:438-448.
Gill SS,Tuteja N.2010.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants.Plant Physiology and Biochemistry,48:909.
Lins RD,Pereira CS,Nenberger PH,et al.2010.Trehaloseprotein interaction in aqueous solution.Proteins:Structure,Function and Bioinformatics,55:177-186.
Matthew P.2007.Trehalose 6-phosphate.Current Opinion in Plant Biology,10:303-309.
Nathan C,Ding A,Nathan C,et al.2010.Snap Shot:Reactive oxygen intermediates(ROI).Cell,140:952.e1.
Pierret A,Doussan C,Capowiez Y,et al.2007.Root functional architecture:A framework for modeling the inter-play between roots and soil.Vadose Zone Journal,6:269-281.
Steinbrenner H,Sies H.2009.Protection against reactive oxygen species by selenoproteins.Biochimica et Biophysica Acta,1790:1478-1485.
Tarek EB,Haluk H,Hüseyin A,et al.2005.Biochemical analysis of trehalose and its metabolizing enzymes in wheat under abiotic stress conditions.Plant Science,169:47-54.
Theerakulpisut P,Gunnula W.2012.Exogenous sorbitol and trehalose mitigated salt stress damage in salt-sensitive but not salt-tolerant rice seedlings.Asian Journal of Crop Science,4:165-170.