水稻无侧根突变体RM109子粒及幼苗生物学特性
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摘要
以水稻无侧根突变体RM109为材料,对其无侧根特性的其它伴随性状进行了研究,旨在揭示遗传突变引起的生理生化变化及其生物学特性。结果表明:突变体的子粒宽、厚、百粒重、百粒谷物量均小于野生型,子粒和发芽第5天的百粒淀粉含量分别是野生型的135%和157%,萌发第4天的幼苗根系琥珀酸脱氢酶(SDH)比活性是野生型的8%;突变体经1μmol·L-1的AgNO3处理后对ACC、H2O2、2,4-D及TIBA都表现出耐性,但对照组突变体(无Ag+处理)对1-氨基环丙烷-1-羧酸(ACC)和H2O2的耐性低于野生型、对2,4-二氯苯氧乙酸(2,4-D)及三碘苯甲酸(TIBA)的耐性高于野生型。
In this paper,the lateral rootless mutant(RM109) was applied to study others accompany-linked traits except the rootless character in order to reveal variation of the physiological and biochemical aspects induced by genetic mutation.Compared to the wild type,some physiological indexes of RM109 were decreased,including grain width,grain thickness,hundred-grain weight,and the amount of hundred-grain.In RM109,Starch content of one hundred grains was 135% of wild type at the seed period and 157% of wild type after germinated for 5 days,while SDH specific activity of root was 8% of wild type after germinated for 4 days.After treated with 1 μmol·L-1AgNO3,RM109 can be tolerant to ACC,H2O2,2,4-D,and TIBA,but the tolerance to ACC and H2O2 with non-Ag+ was lower than that of wild type,and the tolerance to 2,4-D and TIBA with non-Ag+ was higher than that of wild type.
In this paper,the lateral rootless mutant(RM109) was applied to study others accompany-linked traits except the rootless character in order to reveal variation of the physiological and biochemical aspects induced by genetic mutation.Compared to the wild type,some physiological indexes of RM109 were decreased,including grain width,grain thickness,hundred-grain weight,and the amount of hundred-grain.In RM109,Starch content of one hundred grains was 135% of wild type at the seed period and 157% of wild type after germinated for 5 days,while SDH specific activity of root was 8% of wild type after germinated for 4 days.After treated with 1 μmol·L-1AgNO3,RM109 can be tolerant to ACC,H2O2,2,4-D,and TIBA,but the tolerance to ACC and H2O2 with non-Ag+ was lower than that of wild type,and the tolerance to 2,4-D and TIBA with non-Ag+ was higher than that of wild type.
引文
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[14]于龙凤,郝再彬,苍晶.水稻无侧根突变体RM109的显微结构及其根毛形态观察[J].植物生理学通讯,2005,41(3):328-330
[15]郝再彬,苍晶,孙鑫.水稻无侧根突变体RM109的氧化还原代谢[J].核农学报,2002,16(4):193-196
[16]郝再彬,苍晶,孙鑫.水稻无侧根突变体的根向重力性异常[J].植物生理与分子生物学学报,2002,28(3):205-208
[17]王彦超,郝再彬,李子院,李海云,杨丹.直、支链木薯淀粉的分离纯化及检测[J].东北农业大学学报,2009,40(3):47-51
[18]郝再彬,苍晶,徐仲.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社,2004:22
[19]Chhun T,Taketa S,Tsurumi S.The effects of auxin on lateral rootinitiation and root gravitropism in a lateral rootless mutant Lrt1 of rice(Oryza sativa L.)[J].Plant Growth Regulation,2003,39(2):161-170
[20]Wang H,Taketa S,Miyao A,Hirochika H.Isolation of a novellateral-rootless mutant in rice(Oryza sativa L.)with reducedsensitivity to auxin[J].Plant Science,2006,170(1):70-77
[2]Casimiro I,Beeckman T,Graham N,Bhalerao R,Zhang H,Casero P,Sandberg G,Bennett M J.Dissecting Arabidopsis lateral rootdevelopment[J].Trends Plant Science,2003,8(4):165-171
[3]Reed R C,Brady S R,Muday G K.Inhibition of auxin movementfrom the shoot into the root inhibits lateral root development inArabidopsis[J].Plant Physiology,1998,118(4):1369-1378
[4]Celenza J L,Grisafi P L.A pathway for lateral root formation inArabidopsis thaliana[J].Genes Development,1995,9(17):2131-2142
[5]Timpte C,Lincoln C,Pickett F B,Turner J,Estelle M.The AXR1,AUX1 genes of Arabidopsis function in separate auxin-responsepathways[J].The Plant Journal,1995,8(4):561-569
[6]郝再彬,一井真比古.2,4-D耐性を指標に選抜された少側根イネ突然変異体MT10の特性とその遺伝[J].日本作物学会纪事,1997,66(别2号):347-348
[7]Hao Z B,Ichii M.The auxin-resistant mutant of rice with alteredlateral root initiation[M].Japan:Proceedings of the 8th SABRAOGeneral congress.1997:299-300
[8]Hao Z B,Lan X X,Yang L Q,Sun X,Ichii M.Plant hormoneresistance and altered morphology of the MT10 mutant of rice[J].Journal of Northeast Agricultural University,2000,7(2):130-135
[9]Hao Z B,Xu Z,Cang J,Ichii M.MT10 mutant of rice with alteredlateral root initiation[J].Journal of Northeast AgriculturalUniversity,2000,7(1):38-42
[10]郝再彬,一井真比古.无侧根イネ突然变异体RM109特性とその遗传2,4-D耐性を选拔された[J].日本育种学杂志,1997,47:410
[11]郝再彬,一井真比古.无侧根及び重力屈性异常を示す突然变异体RM109の形态特性及び遗传解析[J].日本作物学会纪事,1999,68(2):62-69
[12]郝再彬,苍晶,徐仲,高继国,一井真比古.水稻侧根突变体RM109的农艺性状[J].东北农业大学学报,2001,32(1):75-79
[13]郝再彬.水稻无侧根突变体RM109冠根的解剖学观察[J].核农学报,2002,16(1):15-19
[14]于龙凤,郝再彬,苍晶.水稻无侧根突变体RM109的显微结构及其根毛形态观察[J].植物生理学通讯,2005,41(3):328-330
[15]郝再彬,苍晶,孙鑫.水稻无侧根突变体RM109的氧化还原代谢[J].核农学报,2002,16(4):193-196
[16]郝再彬,苍晶,孙鑫.水稻无侧根突变体的根向重力性异常[J].植物生理与分子生物学学报,2002,28(3):205-208
[17]王彦超,郝再彬,李子院,李海云,杨丹.直、支链木薯淀粉的分离纯化及检测[J].东北农业大学学报,2009,40(3):47-51
[18]郝再彬,苍晶,徐仲.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社,2004:22
[19]Chhun T,Taketa S,Tsurumi S.The effects of auxin on lateral rootinitiation and root gravitropism in a lateral rootless mutant Lrt1 of rice(Oryza sativa L.)[J].Plant Growth Regulation,2003,39(2):161-170
[20]Wang H,Taketa S,Miyao A,Hirochika H.Isolation of a novellateral-rootless mutant in rice(Oryza sativa L.)with reducedsensitivity to auxin[J].Plant Science,2006,170(1):70-77