牙鲆抗淋巴囊肿病家系选育及生长和抗病性能分析
|
摘要
为了选育抗淋巴囊肿的牙鲆(Paralichthys olivaceus)新品种,2015年建立了5个普通家系(C1~C5)、3个雌核发育家系(G1、G2、G3)和1个对照组,在淋巴囊肿病高发养殖场进行自然染毒实验,统计各家系的抗病保护率。同时对各家系120 d、180 d、240 d和300 d时的生长性状进行跟踪测量和比较。结果表明,各家系牙鲆在不同时期有各自不同的生长规律,呈现出家系间生长规律的不一致性。但在所有家系中,家系G2各个时期的生长表现始终排名靠前。390 d统计抗病保护率的结果显示,对照组的抗病保护率只有59.57%,而经选育所有实验组的抗病保护率都在60%以上,最高达97.20%(家系G2)。但总体上,390 d时牙鲆淋巴囊肿抗病和患病个体间体重和体长差异均不显著;体重、体长与抗病保护率为正相关,但相关性均不显著。家系G2抗病个体的平均体重显著高于患病个体(P<0.05)。以G2家系为基础,继续进行选育,有望获得牙鲆抗淋巴囊肿速生新品种。
Lymphocystis disease is a viral infection caused by lymphocystis disease virus, which has serious impacts on the aquaculture industry, resulting in heavy losses. In order to select new varieties of Japanese flounder(Paralichthys olivaceus) resistant to lymphocystis disease, eight experimental groups and one control group were established in 2015. The exposure experiment was conducted on one farm with high incidence of lymphocystis disease, and the protection rate of each group was determined. At the same time, the growth traits of each group at 120 d, 180 d, 240 d, and 300 d were measured and compared. The results showed that there were different growth patterns for each group in different periods, showing inconsistent growth patterns among groups. However, in all the groups, the growth performance of group G2 was always ranked the highest for each period. The results also showed that the disease resistance protection rate of the control group was only 59.57%, while the disease resistance protection rate of all the tested groups was above 60%, reaching up to 97.20% for group G2. From the overall analysis, there were no significant differences in the body weight and body length between the disease resistant or sensitive individuals at 390 d of age. The body weight and body length were positively correlated with disease resistance protection rate, while their effects were not significant(P>0.05). However, the mean body weight of disease-resistant individuals in group G2 was significantly higher than that of disease-sensitive individuals(P< 0.05). Based on the group G2, it is expected that a new variety of fast-growing lymphocystis disease-resistant Japanese flounder could be developed.
Lymphocystis disease is a viral infection caused by lymphocystis disease virus, which has serious impacts on the aquaculture industry, resulting in heavy losses. In order to select new varieties of Japanese flounder(Paralichthys olivaceus) resistant to lymphocystis disease, eight experimental groups and one control group were established in 2015. The exposure experiment was conducted on one farm with high incidence of lymphocystis disease, and the protection rate of each group was determined. At the same time, the growth traits of each group at 120 d, 180 d, 240 d, and 300 d were measured and compared. The results showed that there were different growth patterns for each group in different periods, showing inconsistent growth patterns among groups. However, in all the groups, the growth performance of group G2 was always ranked the highest for each period. The results also showed that the disease resistance protection rate of the control group was only 59.57%, while the disease resistance protection rate of all the tested groups was above 60%, reaching up to 97.20% for group G2. From the overall analysis, there were no significant differences in the body weight and body length between the disease resistant or sensitive individuals at 390 d of age. The body weight and body length were positively correlated with disease resistance protection rate, while their effects were not significant(P>0.05). However, the mean body weight of disease-resistant individuals in group G2 was significantly higher than that of disease-sensitive individuals(P< 0.05). Based on the group G2, it is expected that a new variety of fast-growing lymphocystis disease-resistant Japanese flounder could be developed.
引文
[1]Walker P J,Winton J R.Emerging viral diseases of fish and shrimp[J].Vet Res,2010,41(6):51.
[2]Wolf K.Fish Viruses and Fish Viral Diseases[M].New York:Comstock Publishing Associates,1988.
[3]Zhang Y J.Light and electron microscopic studies of lymphocystis disease in Epinethelus moara[J].Acta Oceanologica Sinica,1992,14(6):97-102.[张永嘉.云纹石斑鱼淋巴囊肿病的光镜和电镜研究[J].海洋学报,1992,14(6):97-102.]
[4]Qu J,Jiang Y L,Sheng H P,et al.A preliminary report on lymphocystis disease in Paralichtys olivaceus[J].China Animal Health Inspection,1998,15(2):4-6.[曲径,江育林,沈海平,等.牙鲆鱼淋巴囊肿病初报[J].中国动物检疫,1998,15(2):4-6.]
[5]Zhang Q Y,Xiao F,Xie J,et al.Complete genome sequence of lymphocystis disease virus isolated from China[J].J Virol,2004,78(13):6982-6994.
[6]Colorni A,Diamant A.Splenic and cardiac lymphocystis in the red drum,Sciaenops ocellatus(L.)[J].J Fish Dis,1995,18(5):467-471.
[7]Shen X Z,Zhan W B.Histopathological studies on target organs of lymphocystis disease virus of fish[J].Periodical of Ocean University of China,2006,36(5):749-753.[绳秀珍,战文斌.鱼类淋巴囊肿病毒靶器官的组织病理研究[J].中国海洋大学学报:自然科学版,2006,36(5):749-753.]
[8]Liu Y K,Sun X Q,Huang J,et al.The diagnostic method of PCR for lymphocystis disease(LCD)of cultured Paralichthys olivaceus[J].Chinese High Technology Letters,2002,12(11):87-89.[刘允坤,孙修勤,黄倢,等.牙鲆淋巴囊肿病的PCR诊断方法研究[J].高技术通讯,2002,12(11):87-89.]
[9]Iwakiri S,Song J,Nakayama K,et al.Host responses of Japanese flounder Paralichthys olivaceus with lymphocystis cell formation[J].Fish Shellfish Immun,2014,38(2):406-411.
[10]Zheng F,Sun X,Liu H,et al.Study on the distribution and expression of a DNA vaccine against lymphocystis disease virus in Japanese flounder(Paralichthys olivaceus)[J].Aquaculture,2006,261(4):1128-1134.
[11]Feng R Z,Xiu Q S,Ming Q X,et al.Immune response of DNA vaccine against lymphocystis disease virus and expression analysis of immune-related genes after vaccination[J].Aquac Res,2010,41(10):1444-1451.
[12]Fuji K,Kobayashi K,Hasegawa O,et al.Identification of a single major genetic locus controlling the resistance to lymphocystis disease in Japanese flounder(Paralichthys olivaceus)[J].Aquaculture,2006,254(1-4):203-210.
[13]Fan C X,Chen S L,Wang L,et al.Screening and identification of SSR markers associated with lymphocystis disease resisrance in Japanese flounder(Paralichthys olivaceus)[J].Journal of Fisheries of China,2014,38(4):576-582.[范彩霞,陈松林,王磊,等.牙鲆抗淋巴囊肿病相关SSR标记的筛选与鉴定[J].水产学报,2014,38(4):576-582.]
[14]Fuji K,Hasegawa O,Honda K,et al.Marker-assisted breeding of a lymphocystis disease-resistant Japanese flounder(Paralichthys olivaceus)[J].Aquaculture,2007,272(1-4):291-295.
[15]Hwang S D,Fuji K,Takano T,et al.Linkage mapping of toll-like receptors(TLRs)in Japanese flounder,Paralichthys olivaceus[J].Mar Biotechnol,2011,13(6):1086-1091.
[16]National Flatfish Industry Technology Development Center.Annual Report 2010 of National Technology System for Flatfish Culture Industry[M].Qingdao:China Ocean University Press,2011.[国家鲆鲽类产业技术研发中心.国家鲆鲽类产业技术体系年度报告[M].青岛:中国海洋大学出版社,2011.]
[17]Overturf K,La Patra S,Towner R,et al.Relationships between growth and disease resistance in rainbow trout,Oncorhynchus mykiss(Walbaum)[J].J Fish Dis,2010,33(4):321-329.
[18]Imsland A K,Jonassen T M,Langston A,et al.The interrelation of growth and disease resistance of different populations of juvenile Atlantic halibut(Hippoglossus hippoglossus L.)[J].Aquaculture,2002,204(1-2):167-177.
[19]Ma A J,Guo J L,Wang X A,et al.Family selection and estimation of disease resistance in turbot,Scophthalmus maximus[J].Journal of Fishery Sciences of China,2014,21(3):484-493.[马爱军,郭建丽,王新安,等.大菱鲆选育家系抗鳗弧菌性能[J].中国水产科学,2014,21(3):484-493.]
[20]Chen S L,Tian Y S,Xu T J,et al.Development and characterization of growth rate and disease resistance of diseaseresistance population and family in Japanese flounder(Paralichthys olivaceus)[J].Journal of Fisheries of China,2008,32(5):665-673.[陈松林,田永胜,徐田军,等.牙鲆抗病群体和家系的建立及其生长和抗病性能初步测定[J].水产学报,2008,32(5):665-673.]
[21]Xu T J,Chen S L,Tian Y S,et al.Comparative analysis of disease resistance among Japanese flounder(Paralichthys olivaceus)families[J].Journal of Fishery Sciences of China,2010,17(1):59-68.[徐田军,陈松林,田永胜,等.牙鲆抗鳗弧菌病家系筛选及其分析[J].中国水产科学,2010,17(1):59-68.]
[22]Wang L,Hu C C,Chen S L,et al.Determination and analysis of 4 breeding traits in Japanese flounder(Paralichthys olivaceus)families[J].Journal of Fishery Sciences of China,2015,22(4):620-629.[王磊,胡灿灿,陈松林,等.牙鲆家系4个选育性状的测定与分析[J].中国水产科学,2015,22(4):620-629.]
[23]Liu H J,Hou J L,Chang Y M,et al.Induced meiogynogenesis in Japanese flounder(Paralichthys olivaceus)by sperm of red sea bream(Pagrus major)[J].Journal of Fisheries of China,2010,34(4):508-514.[刘海金,侯吉伦,常玉梅,等.真鲷精子诱导牙鲆减数分裂雌核发育[J].水产学报,2010,34(4):508-514.]
[24]Li S F,Li C H,Li J L,et al.On-station evaluation of growing performance of five strains of Nile tilapia[J].Journal of Fisheries of China,1998,22(4):28-35.[李思发,李晨虹,李家乐,等.尼罗罗非鱼五品系生长性能评估[J].水产学报,1998,22(4):28-35.]
[25]Kj?glum S,Larsen S,Bakke H G,et al.How specific MHCclass I and class II combinations affect disease resistance against infectious salmon anaemia in Atlantic salmon(Salmo salar)[J].Fish Shellfish Immun,2006,21(4):431-441.
[26]Kj?glum S,Henryon M,Aasmundstad T,et al.Selective breeding can increase resistance of Atlantic salmon to furunculosis,infectious salmon anaemia and infectious pancreatic necrosis[J].Aquac Res,2008,39(5):498-505.
[27]Liu F,Li Y Z,Wang X X,et al.Estimation of genetic parameters for disease-resistance traits in Cynoglossus semilaevis(Günther,1873)[J].J Appl Ichthyol,2016,32(4):643-651.
[28]Xu T J,Chen S L,Ji X S,et al.MHC polymorphism and disease resistance to Vibrio anguillarum in 12 selective Japanese flounder(Paralichthys olivaceus)families[J].Fish Shellfish Immun,2008,25(3):213-221.
[29]Sun H J,Chen S L,Zheng W W,et al.Screening for resistance to Edwardsiella tarda in different families of Japanese flounder,Paralichthys olivaceus[J].Journal of Fishery Sciences of China,2015,22(6):1115-1122.[孙何军,陈松林,郑卫卫,等.抗迟缓爱德华氏菌病牙鲆家系的筛选与分析[J].中国水产科学,2015,22(6):1115-1122.]
[30]Liu Y F,Zhang Y,Wu C X.Selection theories and breeding value prediction of threshold traits[J].Acta Agriculture University Pekineness,1993,19(S1):44-51.[刘月府,张沅,吴常信.阈性状的选择理论和育种值估计方法[J].北京农业大学学报,1993,19(S1):44-51.]
[2]Wolf K.Fish Viruses and Fish Viral Diseases[M].New York:Comstock Publishing Associates,1988.
[3]Zhang Y J.Light and electron microscopic studies of lymphocystis disease in Epinethelus moara[J].Acta Oceanologica Sinica,1992,14(6):97-102.[张永嘉.云纹石斑鱼淋巴囊肿病的光镜和电镜研究[J].海洋学报,1992,14(6):97-102.]
[4]Qu J,Jiang Y L,Sheng H P,et al.A preliminary report on lymphocystis disease in Paralichtys olivaceus[J].China Animal Health Inspection,1998,15(2):4-6.[曲径,江育林,沈海平,等.牙鲆鱼淋巴囊肿病初报[J].中国动物检疫,1998,15(2):4-6.]
[5]Zhang Q Y,Xiao F,Xie J,et al.Complete genome sequence of lymphocystis disease virus isolated from China[J].J Virol,2004,78(13):6982-6994.
[6]Colorni A,Diamant A.Splenic and cardiac lymphocystis in the red drum,Sciaenops ocellatus(L.)[J].J Fish Dis,1995,18(5):467-471.
[7]Shen X Z,Zhan W B.Histopathological studies on target organs of lymphocystis disease virus of fish[J].Periodical of Ocean University of China,2006,36(5):749-753.[绳秀珍,战文斌.鱼类淋巴囊肿病毒靶器官的组织病理研究[J].中国海洋大学学报:自然科学版,2006,36(5):749-753.]
[8]Liu Y K,Sun X Q,Huang J,et al.The diagnostic method of PCR for lymphocystis disease(LCD)of cultured Paralichthys olivaceus[J].Chinese High Technology Letters,2002,12(11):87-89.[刘允坤,孙修勤,黄倢,等.牙鲆淋巴囊肿病的PCR诊断方法研究[J].高技术通讯,2002,12(11):87-89.]
[9]Iwakiri S,Song J,Nakayama K,et al.Host responses of Japanese flounder Paralichthys olivaceus with lymphocystis cell formation[J].Fish Shellfish Immun,2014,38(2):406-411.
[10]Zheng F,Sun X,Liu H,et al.Study on the distribution and expression of a DNA vaccine against lymphocystis disease virus in Japanese flounder(Paralichthys olivaceus)[J].Aquaculture,2006,261(4):1128-1134.
[11]Feng R Z,Xiu Q S,Ming Q X,et al.Immune response of DNA vaccine against lymphocystis disease virus and expression analysis of immune-related genes after vaccination[J].Aquac Res,2010,41(10):1444-1451.
[12]Fuji K,Kobayashi K,Hasegawa O,et al.Identification of a single major genetic locus controlling the resistance to lymphocystis disease in Japanese flounder(Paralichthys olivaceus)[J].Aquaculture,2006,254(1-4):203-210.
[13]Fan C X,Chen S L,Wang L,et al.Screening and identification of SSR markers associated with lymphocystis disease resisrance in Japanese flounder(Paralichthys olivaceus)[J].Journal of Fisheries of China,2014,38(4):576-582.[范彩霞,陈松林,王磊,等.牙鲆抗淋巴囊肿病相关SSR标记的筛选与鉴定[J].水产学报,2014,38(4):576-582.]
[14]Fuji K,Hasegawa O,Honda K,et al.Marker-assisted breeding of a lymphocystis disease-resistant Japanese flounder(Paralichthys olivaceus)[J].Aquaculture,2007,272(1-4):291-295.
[15]Hwang S D,Fuji K,Takano T,et al.Linkage mapping of toll-like receptors(TLRs)in Japanese flounder,Paralichthys olivaceus[J].Mar Biotechnol,2011,13(6):1086-1091.
[16]National Flatfish Industry Technology Development Center.Annual Report 2010 of National Technology System for Flatfish Culture Industry[M].Qingdao:China Ocean University Press,2011.[国家鲆鲽类产业技术研发中心.国家鲆鲽类产业技术体系年度报告[M].青岛:中国海洋大学出版社,2011.]
[17]Overturf K,La Patra S,Towner R,et al.Relationships between growth and disease resistance in rainbow trout,Oncorhynchus mykiss(Walbaum)[J].J Fish Dis,2010,33(4):321-329.
[18]Imsland A K,Jonassen T M,Langston A,et al.The interrelation of growth and disease resistance of different populations of juvenile Atlantic halibut(Hippoglossus hippoglossus L.)[J].Aquaculture,2002,204(1-2):167-177.
[19]Ma A J,Guo J L,Wang X A,et al.Family selection and estimation of disease resistance in turbot,Scophthalmus maximus[J].Journal of Fishery Sciences of China,2014,21(3):484-493.[马爱军,郭建丽,王新安,等.大菱鲆选育家系抗鳗弧菌性能[J].中国水产科学,2014,21(3):484-493.]
[20]Chen S L,Tian Y S,Xu T J,et al.Development and characterization of growth rate and disease resistance of diseaseresistance population and family in Japanese flounder(Paralichthys olivaceus)[J].Journal of Fisheries of China,2008,32(5):665-673.[陈松林,田永胜,徐田军,等.牙鲆抗病群体和家系的建立及其生长和抗病性能初步测定[J].水产学报,2008,32(5):665-673.]
[21]Xu T J,Chen S L,Tian Y S,et al.Comparative analysis of disease resistance among Japanese flounder(Paralichthys olivaceus)families[J].Journal of Fishery Sciences of China,2010,17(1):59-68.[徐田军,陈松林,田永胜,等.牙鲆抗鳗弧菌病家系筛选及其分析[J].中国水产科学,2010,17(1):59-68.]
[22]Wang L,Hu C C,Chen S L,et al.Determination and analysis of 4 breeding traits in Japanese flounder(Paralichthys olivaceus)families[J].Journal of Fishery Sciences of China,2015,22(4):620-629.[王磊,胡灿灿,陈松林,等.牙鲆家系4个选育性状的测定与分析[J].中国水产科学,2015,22(4):620-629.]
[23]Liu H J,Hou J L,Chang Y M,et al.Induced meiogynogenesis in Japanese flounder(Paralichthys olivaceus)by sperm of red sea bream(Pagrus major)[J].Journal of Fisheries of China,2010,34(4):508-514.[刘海金,侯吉伦,常玉梅,等.真鲷精子诱导牙鲆减数分裂雌核发育[J].水产学报,2010,34(4):508-514.]
[24]Li S F,Li C H,Li J L,et al.On-station evaluation of growing performance of five strains of Nile tilapia[J].Journal of Fisheries of China,1998,22(4):28-35.[李思发,李晨虹,李家乐,等.尼罗罗非鱼五品系生长性能评估[J].水产学报,1998,22(4):28-35.]
[25]Kj?glum S,Larsen S,Bakke H G,et al.How specific MHCclass I and class II combinations affect disease resistance against infectious salmon anaemia in Atlantic salmon(Salmo salar)[J].Fish Shellfish Immun,2006,21(4):431-441.
[26]Kj?glum S,Henryon M,Aasmundstad T,et al.Selective breeding can increase resistance of Atlantic salmon to furunculosis,infectious salmon anaemia and infectious pancreatic necrosis[J].Aquac Res,2008,39(5):498-505.
[27]Liu F,Li Y Z,Wang X X,et al.Estimation of genetic parameters for disease-resistance traits in Cynoglossus semilaevis(Günther,1873)[J].J Appl Ichthyol,2016,32(4):643-651.
[28]Xu T J,Chen S L,Ji X S,et al.MHC polymorphism and disease resistance to Vibrio anguillarum in 12 selective Japanese flounder(Paralichthys olivaceus)families[J].Fish Shellfish Immun,2008,25(3):213-221.
[29]Sun H J,Chen S L,Zheng W W,et al.Screening for resistance to Edwardsiella tarda in different families of Japanese flounder,Paralichthys olivaceus[J].Journal of Fishery Sciences of China,2015,22(6):1115-1122.[孙何军,陈松林,郑卫卫,等.抗迟缓爱德华氏菌病牙鲆家系的筛选与分析[J].中国水产科学,2015,22(6):1115-1122.]
[30]Liu Y F,Zhang Y,Wu C X.Selection theories and breeding value prediction of threshold traits[J].Acta Agriculture University Pekineness,1993,19(S1):44-51.[刘月府,张沅,吴常信.阈性状的选择理论和育种值估计方法[J].北京农业大学学报,1993,19(S1):44-51.]