几种工业酶制剂辐照杀菌工艺研究
|
摘要
采用~(60)Co-γ射线辐照处理单宁酶、脂肪酶、α-半乳糖苷酶、葡萄糖氧化酶和氨肽酶5种工业酶制剂,研究不同辐照剂量的杀菌效果及对酶活的影响。结果表明:单宁酶、脂肪酶、葡萄糖氧化酶和氨肽酶在剂量达11.93 kGy时酶活均能保持在对照组85%以上,当辐照剂量达6.17 kGy时,α-半乳糖苷酶酶活减少到对照组89%;辐照处理杀菌效果明显,5种酶制剂的细菌D10值分别从1.30～2.87 kGy不等,D值2.18～6.30 kGy不等。因此,5种工业酶制剂分别在7、3、3、4和5 kGy的辐照剂量下能有效提高产品卫生安全,保证产品质量。
Five industrial enzymes, namely tannase, lipase, α-galactosidase, glucose oxidase and aminopeptidase, were irradiated by ~(60)Co–γ to study the bactericidal effect of different irradiation doses and their effects on enzyme activity. The results showed that the activity of tannase, lipase, glucose oxidase and aminopeptidase could be maintained above 85% of the control group at the dose of 11.93 kGy, while the activity of α-galactosidase decreased to 89% of the control group at the dose of 6.17 kG y. The bactericidal effect of irradiation treatment was obvious. The D10 values of five enzymes ranged from 1.30 to 2.87 kGy, and the D values ranged from 2.18 to 6.30 kGy, respectively. Therefore, five industrial enzymes can effectively improve the hygiene and safety of products and ensure the quality of products at irradiation doses of 7, 3, 3, 4 and 5 kGy, respectively.
Five industrial enzymes, namely tannase, lipase, α-galactosidase, glucose oxidase and aminopeptidase, were irradiated by ~(60)Co–γ to study the bactericidal effect of different irradiation doses and their effects on enzyme activity. The results showed that the activity of tannase, lipase, glucose oxidase and aminopeptidase could be maintained above 85% of the control group at the dose of 11.93 kGy, while the activity of α-galactosidase decreased to 89% of the control group at the dose of 6.17 kG y. The bactericidal effect of irradiation treatment was obvious. The D10 values of five enzymes ranged from 1.30 to 2.87 kGy, and the D values ranged from 2.18 to 6.30 kGy, respectively. Therefore, five industrial enzymes can effectively improve the hygiene and safety of products and ensure the quality of products at irradiation doses of 7, 3, 3, 4 and 5 kGy, respectively.
引文
[1]强新新,马雯雯,王平,等.工业酶的发展现状与展望[J].辽宁化工,2017,46(3):243-247.
[2]于建荣,毛开云,陈大明.工业酶制剂新产品开发和产业化情况分析[J].生物产业技术,2015,(3):53-57.
[3]王睿,刘桂超.论我国酶制剂工业的发展[J].畜牧与饲料科学,2011,32(1):68-69.
[4]刘洪,李文革,胡蝶,等.农药消解酶辐照杀菌研究初报[J].湖南农业科学,2007,(3):49-50.
[5]GB1886.174-2016,食品安全国家标准食品添加剂食品工业用酶制剂[S].
[6]JJG1028-91,中国人民共和国国家计量技术规范使用重铬酸银剂量计测量γ射线水吸收剂量标准方法[S].
[7]GB4789.2-2010,中华人民共和国食品安全国家标准食品微生物学检验菌落总数测定[S].
[8]GB4789.15-2010,中华人民共和国食品安全国家标准食品微生物学检验霉菌和酵母计数[S].
[9]GB4789.3-2016,中华人民共和国食品安全国家标准食品微生物学检验大肠菌群计数[S].
[10]GB4789.4-2010,中华人民共和国食品安全国家标准食品微生物学检验沙门氏菌检验[S].
[11]Libuchi S,Minoda Y,Yamada K.Studies on tannin acyl hydrolaseof microorganisms PartⅡ:A new method determining the enzymeactivity using the change of ultraviolet absorption[J].Agricultural and Biological Chemistry,1967,31(5):513-518.
[12]GB/T23535-2009,中华人民共和国国家标准脂肪酶制剂[S].
[13]董岩岩,张燕婕,黄遵锡,等.产α-半乳糖苷酶菌株的筛选、鉴定及其酶学特性的研究[J].饲料工业,2011,32(14):20-25.
[14]Kouassi G K,Irudayaraj J,Mccarty G.Activity of glucose oxidase functionalized onto magnetic nanoparticles[J].BioMagnetic Research and Technology,2005(3):1.
[15]Zhao G M,Wang Y L,Tian W,et al.Changes of arginyl and leucyl aminopeptidase activities in biceps femoris along Jinhua ham processing[J].Meat Science,2006,74(3):450-458.
[16]陈如燕,蓝俊明,鄢尤奇,等.糖化酶制剂辐照性能研究[J].辐射研究与辐射工艺学报,1992,10(4):223-227.
[17]钱瑞生.γ-射线辐照对α-淀粉酶和糖化酶的影响[J].无锡轻工业学院学报,1992,11(3):192-196.
[18]Kim H J,Chun H H,Song H J,et al.Effects of electron beam irradiation on the microbial growth and quality of beef jerky during storage[J].Radiation Physics and Chemistry,2010,79(11):1165-1168.
[2]于建荣,毛开云,陈大明.工业酶制剂新产品开发和产业化情况分析[J].生物产业技术,2015,(3):53-57.
[3]王睿,刘桂超.论我国酶制剂工业的发展[J].畜牧与饲料科学,2011,32(1):68-69.
[4]刘洪,李文革,胡蝶,等.农药消解酶辐照杀菌研究初报[J].湖南农业科学,2007,(3):49-50.
[5]GB1886.174-2016,食品安全国家标准食品添加剂食品工业用酶制剂[S].
[6]JJG1028-91,中国人民共和国国家计量技术规范使用重铬酸银剂量计测量γ射线水吸收剂量标准方法[S].
[7]GB4789.2-2010,中华人民共和国食品安全国家标准食品微生物学检验菌落总数测定[S].
[8]GB4789.15-2010,中华人民共和国食品安全国家标准食品微生物学检验霉菌和酵母计数[S].
[9]GB4789.3-2016,中华人民共和国食品安全国家标准食品微生物学检验大肠菌群计数[S].
[10]GB4789.4-2010,中华人民共和国食品安全国家标准食品微生物学检验沙门氏菌检验[S].
[11]Libuchi S,Minoda Y,Yamada K.Studies on tannin acyl hydrolaseof microorganisms PartⅡ:A new method determining the enzymeactivity using the change of ultraviolet absorption[J].Agricultural and Biological Chemistry,1967,31(5):513-518.
[12]GB/T23535-2009,中华人民共和国国家标准脂肪酶制剂[S].
[13]董岩岩,张燕婕,黄遵锡,等.产α-半乳糖苷酶菌株的筛选、鉴定及其酶学特性的研究[J].饲料工业,2011,32(14):20-25.
[14]Kouassi G K,Irudayaraj J,Mccarty G.Activity of glucose oxidase functionalized onto magnetic nanoparticles[J].BioMagnetic Research and Technology,2005(3):1.
[15]Zhao G M,Wang Y L,Tian W,et al.Changes of arginyl and leucyl aminopeptidase activities in biceps femoris along Jinhua ham processing[J].Meat Science,2006,74(3):450-458.
[16]陈如燕,蓝俊明,鄢尤奇,等.糖化酶制剂辐照性能研究[J].辐射研究与辐射工艺学报,1992,10(4):223-227.
[17]钱瑞生.γ-射线辐照对α-淀粉酶和糖化酶的影响[J].无锡轻工业学院学报,1992,11(3):192-196.
[18]Kim H J,Chun H H,Song H J,et al.Effects of electron beam irradiation on the microbial growth and quality of beef jerky during storage[J].Radiation Physics and Chemistry,2010,79(11):1165-1168.