春油菜区甘蓝型油菜含油量的杂种优势及配合力研究
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摘要
以6个含油量不同、遗传差距较大的高含油量黑籽甘蓝型油菜为亲本,按照GriffingⅠ完全双列杂交模式Ⅰ配制36个组合(正反交+亲本),在甘肃张掖春油菜区进行随机区组试验,对F1代油菜籽含油量杂种优势及配合力进行分析。结果表明,80.00%的组合表现出正向中亲优势,仅有20.00%的组合表现出正向超亲优势;含油量的广义遗传力为97.83%,狭义遗传力为78.19%;各亲本含油量一般配合力和特殊配合力均存在显著或极显著差异;各组合含油量反交效应达极显著水平。甘蓝型油菜籽含油量的遗传主要受加性基因控制,同时受非加性效应影响;高含油量杂交油菜育种应以一般配合力较高的亲本为主,同时还需考虑组合的正反交差异。
Thirty reciprocal crosses were made with six high-oil black seeded Brassica napus L.lines as parents with different oil content and genetic background,based on the Griffing ModelⅠdesign, and a total of 36accessions including 30reciprocal crosses and six parents were planted in a randomized complete block design in spring rape area in Zhangye of Gansu province.Heterosis and combining ability in oil content were analyzed.The results showed that 80.00%of the crosses exhibited positive mid-parent heterosis,and only 20.00% of the crosses exhibited positive over-parent heterosis.The broad sense heritability of oil content was 97.83%and the narrow sense heritability of oil content was 78.19%.The variances of both general combining ability(GCA)and specific combining ability(SCA) in oil content were significant at 0.05or 0.01level,and the reciprocal cross effect of oil content was highly significant(P<0.01).The analysis showed that the inheritance of oil content in Brassica napus L.was mainly controlled by additive gene effect and affected by non-additive effect simultaneously.It was suggested that high-oil line with high GCA should be used as parents in hybrid rapeseed development for high oil content,moreover,the reciprocal effect should be considered in the determination of spring rapeseed hybrid crosses.
Thirty reciprocal crosses were made with six high-oil black seeded Brassica napus L.lines as parents with different oil content and genetic background,based on the Griffing ModelⅠdesign, and a total of 36accessions including 30reciprocal crosses and six parents were planted in a randomized complete block design in spring rape area in Zhangye of Gansu province.Heterosis and combining ability in oil content were analyzed.The results showed that 80.00%of the crosses exhibited positive mid-parent heterosis,and only 20.00% of the crosses exhibited positive over-parent heterosis.The broad sense heritability of oil content was 97.83%and the narrow sense heritability of oil content was 78.19%.The variances of both general combining ability(GCA)and specific combining ability(SCA) in oil content were significant at 0.05or 0.01level,and the reciprocal cross effect of oil content was highly significant(P<0.01).The analysis showed that the inheritance of oil content in Brassica napus L.was mainly controlled by additive gene effect and affected by non-additive effect simultaneously.It was suggested that high-oil line with high GCA should be used as parents in hybrid rapeseed development for high oil content,moreover,the reciprocal effect should be considered in the determination of spring rapeseed hybrid crosses.
引文
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[12]ZHANG Xiaochun(张晓春),SHI Youming(石有明),YIN Xuewei(尹学伟),et al.Study on yield and quality of rapeseed(Brassica napus L.)in Different Altitude[J].Southwest China Journal of Agricultural Sciences(西南农业学报),2012,25(6):2000-2004(in Chinese with English abstract).
[13]FU Tingdong(傅廷栋).General statement on the studies and utilization of rapeseed heterosis in China[J].Crop Research(作物研究),1990,4(3):1-4(in Chinese with English abstract).
[14]Govil S K,Srivastava A N.Gene action for oil content in Indian mustard[J].Agric Sci,1983,63:404-406.
[15]ZHANG Shufen(张书芬),SONG Wenguang(宋文光),REN Lejian(任乐见),et al.Studies on hereditary capacity of quantitative characters and gene effects of CMS double low Brassica napus[J].Chinese Journal of Oil Crop Sciences(中国油料),1996,18(3):1-3(in Chinese with English abstract).
[16]LI Mei(李梅),CHEN Weijiang(陈卫江).Analysis of combining ability and heritability in major quantitative characters of hybrid rapeseed[J].Hunan Agriculture Sciences(湖南农业科学),2002,7(4):19-21(in Chinese with English abstract).
[17]LI Haojie(李浩杰),PU Xiaobin(浦晓斌),ZHANG Jinfang(张锦芳),et al.Combining ability and genetic ability of main agronomic character s in Brassica napus L.[J].Chinese Agricultural Science Bulletin(中国农学通报),2007,23(6):255-259(in Chinese with English abstract).
[18]WANG Junsheng(王俊生),LI Shaoqin(李少钦).The study on main quantitative characters and genetic ability of high quality parents of hybrid Brassica napus L.[J].Shaanxi Journal Agricutural Sciences(陕西农业科学),2002(1):1-3.
[19]WANG Weirong(王伟荣),LIU Hongjun(刘洪军),FANG Guanhua(方光华),et al.Analysis of heterosis and combining abilities of five rapeseed cultivars(lines)in Brassica napus L.[J].Acta Agriculture Shanghai(上海农业学报),1999,15(2):45-50(in Chinese with English abstract).
[2]HAN Jixiang(韩继祥).Genetic analysis on oil content in rapeseed[J].Chinese Journal of Oil Crop Sciences(中国油料),1990(2):1-6(in Chinese with English abstract).
[3]DAI Wei(戴维),NIU Yingze(牛应泽),GUO Shixing(郭世星),et al.Analysis of heterosis and combining ability in oil content in Brassica napus L.under different environments[J].Southwest China Joumal of Agricultural Sciences(西南农业学报),2008,21(3):581-585(in Chinese with English abstract).
[4]Yadava T P,Gup V P.Heterosis and genetic architecture for oil content in Mustard[J].Genet Plant Breed,1975,35:152-155.
[5]GAO Zhiren(高之仁).Quantitative Genetics(数量遗传学)[M].Chengdu:Sichuan University Press,1986(in Chinese).
[6]TANG Qiyi(唐启义),FENG Mingguang(冯明光).DPS Data Processing System:Experimental Design,Statistical Analysis and Data mining(DPS数据处理系统:试验设计、统计分析及数据处理)[M].Beigjing:Science Press,2007:599-604(in Chinese).
[7]FU Sanxiong(付三雄),QI Cunkou(戚存扣).Major gene plus polygene inheritance of oil content in Brassica napus[J].Jiangsu J of Agr Sci(江苏农业学报),2009,25(4):731-736(in Chinese with English abstract).
[8]Zhang S F,Ma C Z,Zhu J C,et al.Genetic analysis of oil content in Brassica napus L.using mixed model of major gene and polygene[J].Acta Genetica Sinica,2006,33(2):171-180.
[9]ZHANG Jiefu(张洁夫),QI Cunkou(戚存扣),PU Huiming(浦惠明),et al.Inheritance and QTL identification of oil content in rapeseed(Brassica napus L.)[J].Acta Agronomica Ainicca(作物学报),2007,33(9):1495-1501(in Chinese with English abstract).
[10]Wang X F,Liu G H,Yang Q,et al.Genetic analysis on oil content in rapeseed(Brassica napus L.)[J].Euphytica,2010,173(1):17-24.
[11]DONG Yuhong(董育红),TIAN Jianhua(田建华),CHEN Wenjie(陈文杰),et al.Study on heterosis and combining ability in oil content in Brassica napus L.in different ecological regions[J].Journal of Northwest A&F University:Nat Sci Ed(西北农林科技大学学报:自然科学版),2013,41(7):37-42(in Chinese with English abstract).
[12]ZHANG Xiaochun(张晓春),SHI Youming(石有明),YIN Xuewei(尹学伟),et al.Study on yield and quality of rapeseed(Brassica napus L.)in Different Altitude[J].Southwest China Journal of Agricultural Sciences(西南农业学报),2012,25(6):2000-2004(in Chinese with English abstract).
[13]FU Tingdong(傅廷栋).General statement on the studies and utilization of rapeseed heterosis in China[J].Crop Research(作物研究),1990,4(3):1-4(in Chinese with English abstract).
[14]Govil S K,Srivastava A N.Gene action for oil content in Indian mustard[J].Agric Sci,1983,63:404-406.
[15]ZHANG Shufen(张书芬),SONG Wenguang(宋文光),REN Lejian(任乐见),et al.Studies on hereditary capacity of quantitative characters and gene effects of CMS double low Brassica napus[J].Chinese Journal of Oil Crop Sciences(中国油料),1996,18(3):1-3(in Chinese with English abstract).
[16]LI Mei(李梅),CHEN Weijiang(陈卫江).Analysis of combining ability and heritability in major quantitative characters of hybrid rapeseed[J].Hunan Agriculture Sciences(湖南农业科学),2002,7(4):19-21(in Chinese with English abstract).
[17]LI Haojie(李浩杰),PU Xiaobin(浦晓斌),ZHANG Jinfang(张锦芳),et al.Combining ability and genetic ability of main agronomic character s in Brassica napus L.[J].Chinese Agricultural Science Bulletin(中国农学通报),2007,23(6):255-259(in Chinese with English abstract).
[18]WANG Junsheng(王俊生),LI Shaoqin(李少钦).The study on main quantitative characters and genetic ability of high quality parents of hybrid Brassica napus L.[J].Shaanxi Journal Agricutural Sciences(陕西农业科学),2002(1):1-3.
[19]WANG Weirong(王伟荣),LIU Hongjun(刘洪军),FANG Guanhua(方光华),et al.Analysis of heterosis and combining abilities of five rapeseed cultivars(lines)in Brassica napus L.[J].Acta Agriculture Shanghai(上海农业学报),1999,15(2):45-50(in Chinese with English abstract).