煤焦油沥青职业接触工人皮肤损伤相关因素研究
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摘要
目的观察皮肤损伤的煤焦油沥青职业接触工人体内生物标志物的变化。方法采用整群抽样方法,选择职业接触煤焦油沥青且出现皮肤异常的55名工人为皮损组,以职业接触煤焦油沥青且未出现皮肤异常的455名工人为对照组。采集2组人群班后尿及血样,分别进行尿中1-羟基芘、1-羟基萘、2-羟基萘的水平及尾部DNA百分含量、Olive尾矩检测。结果皮损组人群尿中1-羟基芘水平、尾部DNA百分含量、Olive尾矩均高于对照组(中位数:18.78 vs 5.75μg/g肌酐、14.6%vs 12.1%、3.25 vs 2.22μm,P<0.01)。多因素Logistic回归分析显示,尿中1-羟基芘、1-羟基萘、2-羟基萘以及尾部DNA百分含量、Olive尾矩、工龄均是皮肤损伤的影响因素(P<0.05)。结论 1-羟基芘、1-羟基萘、2-羟基萘、尾部DNA百分含量、Olive尾矩均可作为职业接触煤焦油沥青所致皮肤损伤的生物标志物。
引文
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[2] 韩瑜,鲁英,杨晓燕,等.人尿液中4种多环芳烃单羟基代谢产物的液相色谱-串联质谱联用检测法[J].环境与健康杂志,2012,29(10):933-936.
[3] 丁昌明,金银龙,林少彬.固相萃取-液相色谱-串联质谱法同时测定尿液中9种多环芳烃代谢物[J].分析化学,2012,40(3):397-402.
[4] 柳鑫,闫素文.单细胞凝胶电泳在遗传学损伤检测中的应用[J].中国优生与遗传杂志,2003,11(3):9-10.
[5] 黄传峰,闫慧芳,潘祖飞,等.尿中多环芳烃单羟基代谢产物的液相色谱-质谱测定方法研究[J].卫生研究,2010,39(3):355-357.
[6] 徐海娟,冯本秀,杨敏.大鼠微量全血彗星实验方法探讨[J].中国热带医学,2011,11(12):1457-1459.
[7] 魏忠华.PVS防腐技术[J].科技传播,2011(19):148.
[8] 薛春霄,纪玉良,白永利,等.煤焦沥青所致的职业性皮肤病[J].环境与职业医学,2002,19(3):193.
[9] 冯鼎,张俊锋.大同市某煤气公司接触煤焦油工人皮肤损害调查[J].山西医药杂志,2004,33(12):1039.
[10] 李燕,赵德发.石油沥青所致皮肤癌一例报告[J].职业卫生与病伤,2015,30(5):322-323.
[11] LI M,ZHOU Q,TU C,et al.A meta-analysis of an association between the XRCC1 polymorphisms and gliomas risk[J].J Neurooncoal,2013,111(3):221-228.
[12] YANG H Y,YANG S Y,SHAO F Y,et al.Updated assessment of the association of the XRCC1 Arg399Gln polymorphism with lung cancer risk in the Chinese population[J].Asian Pac J Cancer Prev,2015,16(2):495-500.
[13] AKHMADISHINA L Z,GILYAZOVA I R,KUTLYEVA L R,et al.DNA repair XRCC1,XPD genes polymorphism as associated with the development of bladder cancer and renal cell carcinoma[J].Genetika,2014,50(4):421-429.
[14] RAPONI F,BAULEO L,ANCONA C,et al.Quantification of 1-hydroxypyrene,1- and 2-hydroxynaphthalene,3-hydroxybenzo[a]pyrene and 6-hydroxynitropyrene by HPLC-MS/MS in human urine as exposure biomarkers for environmental and occupational surveys[J].Biomarkers,2016:1-9.
[15] 孟潇,段化伟,刘仲,等.焦炉工尿中多环芳烃羟基代谢产物影响因素分析[J].中国职业医学,2017,44(4):425-429.
[16] 边洪英,董一文,王鑫,等.高水平煤焦油沥青接触人员早期症状分析[J].中国职业医学,2017,44(3):382-385.
[17] RAPONI F,BAULEO L,ANCONA C,et al.Quantification of 1-hydroxypyrene,1-and 2-hydroxynaphthalene,3-hydroxybenzo[a]pyrene and 6-hydroxynitropyrene by HPLC-MS/MS in human urine as exposure biomarkers for environmental and occupational surveys[J].Biomarkers,2016:1-9.
[18] YANG P,WANG Y X,CHEN Y J,et al.Urinary polycyclic aromatic hydrocarbon metabolites and human semen quality in China[J].Environ Sci Technol,2017,51(2):958-967.
[19] OLIVEIRA M,SLEZAKOVA K,MAGALH?ES C P,et al.Individual and cumulative impacts of fire emissions and tobacco consumption on wildland firefighters′ total exposure to polycyclic aromatic hydrocarbons[J].J Hazard Mater,2017,334:10-20.
[20] 梁学邈,程子权,孙洪娟,等.钢铁作业工人皮肤患病情况的研究[J].工业卫生与职业病,2005,31(6):379-383.
[21] BURSTYN I,KROMHOUT H,JOHANSEN C,et al.Bladder cancer incidence and exposre to polycyclic aromatic hydrocarbons among asphalt pavers[J].Occup Environ Med,2007,64(8):520-526.
[22] KARAMAN A,PIRIM L.Exposure to bitumen fumes and genotoxic effects on Turkish asphalt workers[J].Clin Toxicol,2009,47(4):321-326.
[23] 王艳华.焦炉逸散物致呼吸系统损伤与基因组DNA甲基化改变的生物标志物研究[D].北京:中国疾病预防控制中心,2017.
[24] 梁宗英,张乐,孙光蕊,等.煤焦油沥青烟气诱导 SD 大鼠肺癌动物模型的实验研究[J].临床和实验医学杂志,2015,14(22):1844-1845.