火麻仁油藻油混合物安全性实验研究
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摘要
目的评价火麻仁油藻油混合物安全性。方法采用KM小鼠和SD大鼠为实验动物,分别进行急性毒性试验、遗传毒性试验以及30d喂养试验。结果急性毒性试验中,样品组与阴性对照组比较,自发活动、反应速度、精神状况、组织器官等均无异常(P>0.05);遗传毒性试验中,小鼠骨髓嗜多染红细胞微核含量测定,样品各剂量组与阴性对照组比较,差异无显著性(P>0.05);小鼠精子畸形试验中,样品各剂量组与阴性对照组比较无显著性差异(P>0.05);30d喂养试验中,各剂量组大鼠体重增重、进食量及食物利用率与对照组比较无显著性差异(P>0.05),血生化指标和血常规指标与对照组比较无显著性差异(P>0.05)。结论在此次的实验中,未发现火麻仁油藻油有毒性作用,为无毒物。
Objective To evaluate the safety of hemp seed oil and algae oil mixture.Methods Acute toxicity test,Ames test,sperm shape abnormality in KM mice,30 d feeding test in SD rats were used to assess the safety of the mixture oil.Results Compared with the control group,the spontaneous activity,the reaction rate,the mental state,the organization and the organs revenled no abnormalities in acute toxicity test(P>0.05).The Results of Ames test,micronucleus test of bone marrow and sperm shape abnormality in mice were negative(P>0.05).There was no significant difference in body weight gain,food intake and utilization between the two groups in 30 d feeding test(P>0.05).No obvious effects were observed at blood biochemical indexes and blood routine indexes in control group(P>0.05).Conclusion No adverse effects were observed in the series of toxicity tests in this experiment and the mixture oil could be non-toxic.
Objective To evaluate the safety of hemp seed oil and algae oil mixture.Methods Acute toxicity test,Ames test,sperm shape abnormality in KM mice,30 d feeding test in SD rats were used to assess the safety of the mixture oil.Results Compared with the control group,the spontaneous activity,the reaction rate,the mental state,the organization and the organs revenled no abnormalities in acute toxicity test(P>0.05).The Results of Ames test,micronucleus test of bone marrow and sperm shape abnormality in mice were negative(P>0.05).There was no significant difference in body weight gain,food intake and utilization between the two groups in 30 d feeding test(P>0.05).No obvious effects were observed at blood biochemical indexes and blood routine indexes in control group(P>0.05).Conclusion No adverse effects were observed in the series of toxicity tests in this experiment and the mixture oil could be non-toxic.
引文
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[11]张坚,孟丽萍,姜元荣,等.中国成人膳食脂肪酸摄入和食品来源状况分析[J].营养学报,2009,31(5):421-427.
[2]贺海波,石孟琼.火麻仁的化学成分和药理活性研究进展[J].中国民族民间医药,2010,19(15):56-57.
[3]温雪馨,李建平,侯文伟,等.微藻DHA的营养保健功能及在食品工业中的应用[J].食品科学,2010,31(21):446-450.
[4]杨贤庆,吕军伟,林婉玲,等.DHA功能特性以及抗氧化性研究进展[J].食品工业科技,2014,69(2):390-394.
[5]卫生部国家标准化管理委员会.GB15193-2003食品安全性毒理学评价程序和方法[M].北京:中国标准出版社,2004.
[6]蒋平,杨阳,孙阳,等.养胃颗粒灌胃给药对小鼠的急性毒性实验[J].时珍国医国药,2014(4):841-843.
[7]扈学俸,李永进,王军波,等.火麻仁油安全性评价及血清抗氧化功能初步研究[J].中国食品卫生杂志[J].2008,20(5):388-392.
[8]苏婧,贺海波,石孟琼,等.火麻仁油对D-半乳糖致衰老小鼠学习记忆障碍的保护作用研究[J].中国临床药理学与治疗学,2011,16(12):1332-1339.
[9]吴克刚,曹建华.海藻油的氧化稳定性及其微胶囊化研究[J].中国海洋药物,2004,23(4):35-38,58.
[10]郝颖,汪之和.EPA、DHA的营养功能及其产品安全性分析[J].现代食品科技,2006,22(3):180-183.
[11]张坚,孟丽萍,姜元荣,等.中国成人膳食脂肪酸摄入和食品来源状况分析[J].营养学报,2009,31(5):421-427.