保健食品中EPA、DHA和α-亚麻酸测定方法改进
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摘要
目的在现有测定方法上,改进保健食品中测定EPA、DHA和α-亚麻酸的前处理方法,并进行方法学研究。方法样品经皂化、甲酯化后加正己烷定容,加氯化钠溶液混匀后离心静置,取上清液测试,经DB-FFAP毛细管色谱柱分离测定。结果该方法在50-850μg·mL-1间具有良好的线性(r>0.999),检出限为0.8～1.5mg·kg-1,回收率90%～105%,相对标准偏差<5%。结论本方法可用于鱼油保健品中EPA、DHA和α-亚麻酸的同时测定,操作简单快捷,结果可靠,重现性高。
OBJECTIVE To optimize the pretreatment methods for simultaneous determination of EPA(Eicosapentaenoic acid),DHA(Docosahexaenoic acid) and α-Linolenic acid in fish oil by GC,and study on methodology.METHODS DHA,EPA and α-Linolenic acid were extracted by saponification and esterification,added hexane to the mark,then added sodium chloride solution,mixed,centrifuged and stand seconds,analyzed by gas chromatography coupled with DB-FFAP capillary column used to separate the fatty acids.RESULTS The mass concentration of DHA,EPA and α-Linolenic acid were linear with peak area in the range of 50～850μg·mL-1,and regression coeficient was not less than 0.999.The average rate ranged from 90% to 105%,and the relative standard deviation was not more than 5%(n=3).CONCLUSION The method is simple and convenient,it can be used to determine the content of DHA,EPA and α-Linolenic acid in fish oils with good accuracy and repeatability.
OBJECTIVE To optimize the pretreatment methods for simultaneous determination of EPA(Eicosapentaenoic acid),DHA(Docosahexaenoic acid) and α-Linolenic acid in fish oil by GC,and study on methodology.METHODS DHA,EPA and α-Linolenic acid were extracted by saponification and esterification,added hexane to the mark,then added sodium chloride solution,mixed,centrifuged and stand seconds,analyzed by gas chromatography coupled with DB-FFAP capillary column used to separate the fatty acids.RESULTS The mass concentration of DHA,EPA and α-Linolenic acid were linear with peak area in the range of 50～850μg·mL-1,and regression coeficient was not less than 0.999.The average rate ranged from 90% to 105%,and the relative standard deviation was not more than 5%(n=3).CONCLUSION The method is simple and convenient,it can be used to determine the content of DHA,EPA and α-Linolenic acid in fish oils with good accuracy and repeatability.
引文
[1]郑秋甫.Omega-3多不饱和脂肪酸的研究进展[J].中华保健医学杂志,2011,13(5):357-360.
[2]Kim K,Nam Y A,Kim H S,et al.alpha-Linolenic acid:Nutraceutical,pharmacological and toxicological evaluation[J].Food and Chemical Toxicology,2014,(70):163-178.
[3]温雪馨,李建平,侯文伟,等.微藻DHA的营养保健功能及在食品工业中的应用[J].食品科学,2010,31(21):446-450.
[4]巫小丹,黎紫含,张珊珊,等.二十二碳五烯酸代谢和功能研究进展[J].中国油脂,2016,41(6):44-47.
[5]蔡伟江,张喜金,苏昭仑.气相色谱法测定鱼油中的二十碳五烯酸、二十二碳六烯酸和二十二碳五烯酸[J].食品安全质量检测学报,2015,6(5):1924-1928.
[6]宋家玉,陈金东,刘文杰,等.毛细管气相色谱法测定鱼油食品中的脂肪酸DHA,EPA[J].化学分析计量,2013,22(4):53-56.
[7]李妍,王静,李麒龙,等.EPA与DHA最新研究进展[A].农产品加工(学刊),2013,(2):6-13.
[8]邓泽元,余迎利,John KGK.GC和HPLC对共轭亚油酸的测定[J].中国油脂,2005,30(3):36-39.
[9]鲁辉,周旋,钱叶飞,等.保健食品中EPA和DHA含量测定方法研究[J].中国食品添加剂,2015,4:190-194.
[10]牟峻,曲忠文.鱼油制品中EPA/DHA检测方法的研究[J].食品科学,2001,22(10):76-78.
[11]薛秀恒,王志耕,王菊花.牛奶中共轭亚油酸含量的紫外分光光度检测方法[J].分析检测,2005,7:176-178.
[12]赵雷,常辉,胡金山,等.毛细管电泳在食用油脂脂肪酸检测中的应用[J].农产品加工(学刊),2014,18:190-194.
[13]可成友,吴晓芳.不饱和脂肪酸的气相色谱法同时测定[J].中国卫生检验杂志,2005,5(15):528-530.
[14]陈亚飞,程速远,孙宇,等.毛细管气相色谱法测定海狗油胶丸中ω-3多不饱和脂肪酸的含量[J].药物分析杂志,2004,24(5):499-501.
[15]中华人民共和国卫生部.GB 5009.168-2016食品安全国家标准食品中脂肪酸的测定[S].北京:中国标准出版社,2016.
[16]中华人民共和国卫生部.GB 28404-2012食品安全国家标准保健食品中α-亚麻酸、二十碳五烯酸、二十二碳五烯酸和二十二碳六烯酸的测定[S].北京:中国标准出版社,2012.
[17]吴俏槿,杜冰,蔡尤林,等.α-亚麻酸的生理功能及开发研究进展[J].食品工业科,2016,10:386-390.
[2]Kim K,Nam Y A,Kim H S,et al.alpha-Linolenic acid:Nutraceutical,pharmacological and toxicological evaluation[J].Food and Chemical Toxicology,2014,(70):163-178.
[3]温雪馨,李建平,侯文伟,等.微藻DHA的营养保健功能及在食品工业中的应用[J].食品科学,2010,31(21):446-450.
[4]巫小丹,黎紫含,张珊珊,等.二十二碳五烯酸代谢和功能研究进展[J].中国油脂,2016,41(6):44-47.
[5]蔡伟江,张喜金,苏昭仑.气相色谱法测定鱼油中的二十碳五烯酸、二十二碳六烯酸和二十二碳五烯酸[J].食品安全质量检测学报,2015,6(5):1924-1928.
[6]宋家玉,陈金东,刘文杰,等.毛细管气相色谱法测定鱼油食品中的脂肪酸DHA,EPA[J].化学分析计量,2013,22(4):53-56.
[7]李妍,王静,李麒龙,等.EPA与DHA最新研究进展[A].农产品加工(学刊),2013,(2):6-13.
[8]邓泽元,余迎利,John KGK.GC和HPLC对共轭亚油酸的测定[J].中国油脂,2005,30(3):36-39.
[9]鲁辉,周旋,钱叶飞,等.保健食品中EPA和DHA含量测定方法研究[J].中国食品添加剂,2015,4:190-194.
[10]牟峻,曲忠文.鱼油制品中EPA/DHA检测方法的研究[J].食品科学,2001,22(10):76-78.
[11]薛秀恒,王志耕,王菊花.牛奶中共轭亚油酸含量的紫外分光光度检测方法[J].分析检测,2005,7:176-178.
[12]赵雷,常辉,胡金山,等.毛细管电泳在食用油脂脂肪酸检测中的应用[J].农产品加工(学刊),2014,18:190-194.
[13]可成友,吴晓芳.不饱和脂肪酸的气相色谱法同时测定[J].中国卫生检验杂志,2005,5(15):528-530.
[14]陈亚飞,程速远,孙宇,等.毛细管气相色谱法测定海狗油胶丸中ω-3多不饱和脂肪酸的含量[J].药物分析杂志,2004,24(5):499-501.
[15]中华人民共和国卫生部.GB 5009.168-2016食品安全国家标准食品中脂肪酸的测定[S].北京:中国标准出版社,2016.
[16]中华人民共和国卫生部.GB 28404-2012食品安全国家标准保健食品中α-亚麻酸、二十碳五烯酸、二十二碳五烯酸和二十二碳六烯酸的测定[S].北京:中国标准出版社,2012.
[17]吴俏槿,杜冰,蔡尤林,等.α-亚麻酸的生理功能及开发研究进展[J].食品工业科,2016,10:386-390.