高脂饮食诱导建立小鼠高脂血症模型
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摘要
目的探讨不同配方的高脂饮食对2种不同种系小鼠的高脂血症成模的影响。方法用4种不同配方的高脂饲料对昆明种小鼠和C57BL/6小鼠长期喂饲后,考察体质量和内脏脂肪含量,检测血和肝脏中三酰甘油和总胆固醇的水平。结果不同配方的高脂饲料均可使2种小鼠血浆中总胆固醇水平升高(高脂饲料1对昆明种小鼠:1 892±83mg·L-1vs 691±32mg·L-1,P<0.01),但却降低了小鼠血浆中三酰甘油水平(高脂饲料1对昆明种小鼠:1 125±116mg·L-1vs 2 061±138mg·L-1,P<0.01),高脂饲料中胆固醇和胆酸钠的有无与小鼠肝脏中总胆固醇和三酰甘油水平呈明显正相关,与血浆中三酰甘油水平呈负相关,对血浆胆固醇的水平影响不大。结论采用高脂饮食长期喂饲能成功建立小鼠高胆固醇血症模型,但无法建立小鼠高三酰甘油血症模型。
Objective To study the effects of different high fat diets(HFD)on hyperlipidemic models in 2strains mice.Methods To feed C57BL/6and Kunming mice with 4different HFD for several weeks,then body weights,visceral fats,total cholesterol(TC)and triglyce ride(TG)in blood and in livers were measured.Results 4different HFD could increase blood TC,liver TC and liver TG in 2stains mice(blood TC in Kunming mice induced by type 1HFD and by normal diet:1 892±83mg·L-1 vs 691±32mg·L-1,P<0.01)and inhibited blood TG levels in C57BL/6and Kunming mice(blood TG in Kunming mice induced by type 1HFD and by normal diet:1 125±116mg·L-1 vs 2 061±138mg·L-1,P<0.01).Cholesterol and sodium cholate in HFD positively regulated liver TC and liver TG and negatively regulated blood TG level in Kunming mice.Conclusion Hypercholesterolemia could be successfully established in mice fed with HFD,however,hypertriglyceridemia could not be induced by HFD in C57BL/6and Kunming mice.
Objective To study the effects of different high fat diets(HFD)on hyperlipidemic models in 2strains mice.Methods To feed C57BL/6and Kunming mice with 4different HFD for several weeks,then body weights,visceral fats,total cholesterol(TC)and triglyce ride(TG)in blood and in livers were measured.Results 4different HFD could increase blood TC,liver TC and liver TG in 2stains mice(blood TC in Kunming mice induced by type 1HFD and by normal diet:1 892±83mg·L-1 vs 691±32mg·L-1,P<0.01)and inhibited blood TG levels in C57BL/6and Kunming mice(blood TG in Kunming mice induced by type 1HFD and by normal diet:1 125±116mg·L-1 vs 2 061±138mg·L-1,P<0.01).Cholesterol and sodium cholate in HFD positively regulated liver TC and liver TG and negatively regulated blood TG level in Kunming mice.Conclusion Hypercholesterolemia could be successfully established in mice fed with HFD,however,hypertriglyceridemia could not be induced by HFD in C57BL/6and Kunming mice.
引文
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[5]周淑芳,李晶,关溯,等.四氢普伐他汀钠对高血脂模型新西兰兔脂质代谢的影响[J].西北药学杂志,2016,31(1):51-56.
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[11]吕建敏,应华忠,徐孝平,等.高脂血症动物模型研究进展[J].浙江中医学院学报,2005,29(4):87-89.
[12]侯杰军,闫曙光,付强,等.大鼠高脂血症模型不同配方的实验研究[J].陕西中医学院学报,2009,32(1):48-49.
[13]张智,闪增郁,向丽华,等.大鼠实验性高脂血症两种造模方法的比较[J].中国中医基础医学杂志,2004,10(2):33-34,24.
[14]张少芬,陈方,吴铁,等.长期脂肪乳剂灌胃致小鼠高脂血症模型的研究[J].广东医学院学报,2004,22(4):328-330.
[2]朱竟赫,赵金明,秦文艳,等.高脂血症实验动物模型研究概述[J].实验动物科学,2012,29(2):48-52.
[3]高敏,倪凯,杨玉琪.高脂血症动物模型的研究概况[J].云南中医中药杂志,2010,31(10):79-81.
[4]英提扎尔·艾孜木江,阿依姑丽·艾合麦提,李珊,等.野山杏果肉中总有机酸对高脂血症大鼠体内抗氧化作用研究[J].西北药学杂志,2018,33(3):359-363.
[5]周淑芳,李晶,关溯,等.四氢普伐他汀钠对高血脂模型新西兰兔脂质代谢的影响[J].西北药学杂志,2016,31(1):51-56.
[6] Kris-Etherton P M,Dietschy J.Design criteria for studies examining individual fatty acid effects on cardiovascular disease risk factors:human and animal studies[J].Am J Clin Nutr,1997,65(5Suppl):1590S-1596S.
[7] Marotti K R,Castle C K,Boyle T P,et al.Severe atherosclerosis in transgenic miceexpressing simian cholesteryl ester transfer protein[J].Nature,1993,364(6432):73-75.
[8]杨健,周群.高脂血症模型的研究概况[J].湖南中医杂志,2013,29(4):132-133.
[9] Moghadasian M H,Frohlich J J,McManus B M.Advances in experimental dyslipidemia and atherosclerosis[J].Lab Invest,2001,81(9):1173-1183.
[10]梁红峰,陈方.高脂血症模型小鼠生化指标的变化[J].广东微量元素科学,2004,11(4):42-45.
[11]吕建敏,应华忠,徐孝平,等.高脂血症动物模型研究进展[J].浙江中医学院学报,2005,29(4):87-89.
[12]侯杰军,闫曙光,付强,等.大鼠高脂血症模型不同配方的实验研究[J].陕西中医学院学报,2009,32(1):48-49.
[13]张智,闪增郁,向丽华,等.大鼠实验性高脂血症两种造模方法的比较[J].中国中医基础医学杂志,2004,10(2):33-34,24.
[14]张少芬,陈方,吴铁,等.长期脂肪乳剂灌胃致小鼠高脂血症模型的研究[J].广东医学院学报,2004,22(4):328-330.