管花肉苁蓉苯乙醇苷水溶媒提取技术研究
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摘要
以管花肉苁蓉为原料,探究原料粒径、料液比、浸提温度、时间等因素对苯乙醇苷浸提效果,开发苯乙醇苷水溶媒提取技术。经单因素优化及正交试验优化,管花肉苁蓉苯乙醇苷水溶媒提取最佳工艺为:原料粒径>40目,料液比1:15,浸提温度80℃,浸提时间2 h,提取率为(175.9±1.1)mg/g,松果菊苷及毛蕊花糖苷分别占总苯乙醇苷的38.85%、8.78%。研究结果为管花肉苁蓉苯乙醇苷提取降低成本及安全生产提供了参考依据。
In order to develop water solvent extraction technology of phenylethanoid glycoside from Cistanche tubulosa(Schenk) R. Wight, the influences of particle size, solid-to-liquid ratio, extraction temperature, extraction time were been studied. Through single factor optimization and orthogonal test,the optimum extraction condition are mesh number>40, solid-to-liquid ratio of 1:15, temperature 80 ℃,extract for 2 h. Under these conditions, the extraction yield of phenylethanoid glycoside reached(175.9±1.1)mg/g, the content of echinacoside and acteoside are 38.85% and 8.78% respectively. This study provides a theoretic basis for the lowcost and safty production of phenylethanoid glycoside.
In order to develop water solvent extraction technology of phenylethanoid glycoside from Cistanche tubulosa(Schenk) R. Wight, the influences of particle size, solid-to-liquid ratio, extraction temperature, extraction time were been studied. Through single factor optimization and orthogonal test,the optimum extraction condition are mesh number>40, solid-to-liquid ratio of 1:15, temperature 80 ℃,extract for 2 h. Under these conditions, the extraction yield of phenylethanoid glycoside reached(175.9±1.1)mg/g, the content of echinacoside and acteoside are 38.85% and 8.78% respectively. This study provides a theoretic basis for the lowcost and safty production of phenylethanoid glycoside.
引文
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[2]FU Zhifei,FAN Xiang,WANG Xiaoying,et al.Cistanches Herba:An overview of its chemistry,pharmacology,and pharmacokinetics property[J].Journal of Ethnopharmacology,2018,219:233﹣247.
[3]SONG Xun,LI Chenyang,ZENG Yong,et al.Immuno﹣modulatory effects of crude phenylethanoid glycosides from Ligustrum purpurascens[J].Journal of Ethno﹣pharmacology,2012,144:584﹣591.
[4]陶义存,李建英,许永华,等.肉苁蓉苯乙醇苷对大鼠高原肺水肿的防治作用[J].中国实验方剂学杂志,2014,20(15):134﹣138.
[5]ZHANG Yanjun,WANG Ke,CHEN Huangcan,et al.Anti-inflammatory lignans and phenylethanoid glycosides from the root of Isodon ternifolius(D.Don)Kud?[J].Phytochemistry,2018,153:36﹣47.
[6]宋光西,马玲云,魏锋,等.苯乙醇苷的分布及药理活性研究进展[J].亚太传统医药,2011,7(4):169﹣171.
[7]WANG Lin﹣lin,DING Hui,YU Heshui,et al.Cistanches Herba:Chemical Constituents and Pharmacological Effects[J].Chinese Herbal Medicines,2015,7(2):135﹣142.
[8]孟胜喜,霍清萍.肉苁蓉有效成分对神经系统作用研究进展[J].中国中医药信息杂质,2016,23(10):123﹣126.
[9]丁慧玲,许丹丹,金天佑,等.超声﹣微波回流提取新疆管花肉苁蓉苯乙醇苷的工艺研究[J].食品工业科技,2016,37(12):301﹣305.
[10]仵红磊,程卫东,邹圣东.管花肉苁蓉苯乙醇苷不同提取方法的比较研究[J].食品工业,2010,(5):64﹣66.
[11]覃文婷,宿美凤,雒晓梅,等.定性分析不同产地荒漠肉苁蓉苯乙醇苷类及多糖类成分[J].辽宁中医药大学学报,2018,20(7):77﹣81.
[12]李蒙,付艳秋,韩静,等.响应面法优化肉苁蓉苯乙醇苷回流提取工艺[J].中南药学,2015,13(8):819﹣823.
[13]刘伯言.肉苁蓉苯乙醇苷的提取、纯化和抗氧化活性研究[D].北京:北京林业大学,2014:13﹣15.
[14]梁淑燕,卢丹逸,耿宗成,等.提高肉苁蓉中苯乙醇苷类成分含量的加工方法研究[J].中国现代中药,2017,19(7):1026﹣1029.
[15]王丽楠,陈君,杨美华,等.不同初加工温度对肉苁蓉有效成分含量的影响[J].中国药房,2007,21(18):1620﹣1623.
[16]宋青青,刘瑶,张玲玲,等.在线加压溶剂微提取﹣湍流色谱﹣高效液相色谱法同时测定管花肉苁蓉中3种苯乙醇苷[J].色谱,2016,34(6):572﹣576.
[17]杨太新,杜艳华,刘金娜,等.管花肉苁蓉不同生长时间和部位的有效成分含量分析[J].时珍国医国药,2014,25(5):1191﹣1193.
[18]吴爱芝,林朝展,祝晨蔯.苯乙醇苷类成分构效关系研究进展[J].天然产物研究与开发,2013,25:862﹣865.