银屑病患者真皮间充质干细胞中细胞因子受体因子-1白细胞介素-6水平的变化及意义
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摘要
目的检测细胞因子受体因子-1(CRLF1)、白细胞介素(IL)-6在寻常性银屑病患者皮损处真皮间充质干细胞(DMSCs)中的表达,并分析其与银屑病发病的关系。方法采用荧光定量聚合酶链反应(PCR)法测定15例银屑病患者皮损处DMSCs内CRLF1、IL-6水平的变化,并与银屑病皮损面积及严重度指数评分做相关性分析。另取15例健康的皮肤组织作为正常对照组。结果 (1)银屑病患者组CRLF1、IL-6水平低于正常对照组;(2)CRLF1、IL-6与银屑病皮损面积及严重度指数评分无明显相关性。结论 CRLF1、IL-6表达异常可能与银屑病免疫学JAK/STAT信号途径有关。
Objective To examine the expression levels of cytokine receptor-like factor 1(CRLF1) and interleukin-6(IL-6) in dermal mesenchymal stem cells(DMSCs) of patients with psoriasis vulgaris,and to determine their relationship with pathogenesis of psoriasis.Methods The changes of CRLF1 and IL-6 levels in DMSCs at skin lesions of 15 patients with psoriasis were determined by fluorescence quantitative PCR.The correlations analysis between the changes of levels and the area of psoriasis lesions and severity index scores were determined,respectively.Another15 normal subjects were included as the control group.Results(1) The levels of CRLF1 and IL-6 in the psoriasis group were lower than those in the normal control group.(2) There was no significant correlation between the levels of CRLF1 and IL-6 and the area of psoriasis lesions and severity index scores,respectively.Conclusion The abnormal expression of CRLF1 and IL-6 may be related to the immunological JAK/STAT signaling pathway of psoriasis.
Objective To examine the expression levels of cytokine receptor-like factor 1(CRLF1) and interleukin-6(IL-6) in dermal mesenchymal stem cells(DMSCs) of patients with psoriasis vulgaris,and to determine their relationship with pathogenesis of psoriasis.Methods The changes of CRLF1 and IL-6 levels in DMSCs at skin lesions of 15 patients with psoriasis were determined by fluorescence quantitative PCR.The correlations analysis between the changes of levels and the area of psoriasis lesions and severity index scores were determined,respectively.Another15 normal subjects were included as the control group.Results(1) The levels of CRLF1 and IL-6 in the psoriasis group were lower than those in the normal control group.(2) There was no significant correlation between the levels of CRLF1 and IL-6 and the area of psoriasis lesions and severity index scores,respectively.Conclusion The abnormal expression of CRLF1 and IL-6 may be related to the immunological JAK/STAT signaling pathway of psoriasis.
引文
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[13]Forger NG,Prevette D,De Lapeyriere O,et al.Cardiotrophin like cytokine/cytokine-like factor 1 is an essential trophic factor for lumbar and facial motoneurons in vivo[J].J Neurosci,2003,23(26):8854-8858.
[14]Heinrich PC,Behrmann IS,Hermanns HM,et al.Principles of in-terleukin(IL)-6-type cytokine signalling and its regulation[J].Biochem J,2003,374(Pt 1):1-20.
[15]Davis S,Aldrich TH,Stahl N,et al.LIFRβand gp130 as heterodimerizing signal transducers of the tripartite CNTF receptor[J].Science,1993,260:1805-1808.
[2]Liu RF,Wang Y,Zhao XC,et al.Lymphocyte inhibition is com-promised in mesenchymal stem cells from psoriatic skin[J].Eur J Dermatol,2014,24(5):560-567.
[3]Campanati A,Orciani M,Gorbi S,et al.Effect of biologic thera-pies targeting tumour necrosis factor-αon cutaneous mesenchy-mal stem cells in psoriasis[J].Br J Dermatol,2012,167(1):68-76.
[4]Livak KJ,Schmittgen TD.Analysis of relative gene expression da-ta using real-time quantitative PCR and the 2(-Delta Delta CT)method[J].Methods,2001,25(4):402-408.
[5]刘瑞风,杨元文,赵新程,等.银屑病患者皮损间充质干细胞分泌表皮生长因子、干细胞因子、碱性成纤维细胞生长因子、血管内皮生长因子水平检测[J].中国免疫学杂志,2013,10(29):1046-1049.
[6]Liu H,Moroi Y,Yasumoto S,et al.Immunohistochemical local-ization of activated Stat3 and h TERT protein in psoriasis vulgaris[J].Eur J Dermatol,2006,16(2):205-207.
[7]Vrontaki E,Melagraki G,Afantitis A,et al.Searching for novel Janus kinase-2 inhibitors using a combination of pharmacophore modeling,3D-QSAR studies and virtual screening[J].Mini Rev Med Chem,2016,16(3):268-294.
[8]Juncheng W,Yanzhi Y,Chaozhi J.The Ubiquitin ligase TRAF6 negatively regulates the JAK-STAT signaling pathway by bind-ing to STAT3 and mediating its ubiquitination[J].PLo S ONE,2012,7(11):e49567.
[9]Sano S,Chan KS,Carbajal S,et al.Stat3 links activated keratinocytes and immunocytes required for development of pso-riasis in a novel transgenic mouse model[J].Nat Med,2005,11(1):43-49.
[10]Sabry A,Sheashaa H,El-Husseini A,et al.Proinflammatory cy-tokines(TNF-alpha and IL-6)in Egyptian patientswith SLE:its correlation with disease activity[J].Cytokine,2006,35(3-4):148-153.
[11]Shi Y,Wang W,Yourey PA,et al.Computational EST database analysis identifies a novel member of the neuropoietic cytokine family[J].Biochem Biophys Res Commun,1999,262(1):132-138.
[12]Senaldi G,Varnum BC,Sarmiento U,et al.Novel neurotrophin-1/B cell-stimulating factor-3:a cytokine of the IL-6 family[J].Proc Natl Acad Sci USA,1999,96(20):11458-11463.
[13]Forger NG,Prevette D,De Lapeyriere O,et al.Cardiotrophin like cytokine/cytokine-like factor 1 is an essential trophic factor for lumbar and facial motoneurons in vivo[J].J Neurosci,2003,23(26):8854-8858.
[14]Heinrich PC,Behrmann IS,Hermanns HM,et al.Principles of in-terleukin(IL)-6-type cytokine signalling and its regulation[J].Biochem J,2003,374(Pt 1):1-20.
[15]Davis S,Aldrich TH,Stahl N,et al.LIFRβand gp130 as heterodimerizing signal transducers of the tripartite CNTF receptor[J].Science,1993,260:1805-1808.