硬齿猕猴桃组织培养和快速繁殖技术研究
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摘要
以种子、茎段为外植体,对野生种硬齿猕猴桃进行了组织培养和快速繁殖技术的研究。结果表明:在初代培养阶段,一定浓度的激素6-BA、ZT、IBA对硬齿猕猴桃的种子萌发无促进作用,不加激素的MS基本培养基是硬齿猕猴桃最佳的种子启动培养基; MS+0.5 mg/L 6-BA+0.2 mg/L IBA可作为硬齿猕猴桃较佳的茎段启动培养基;在继代培养阶段, MS+2.0 mg/L 6-BA+0.2 mg/L IBA较有利于硬齿猕猴桃的增殖;1/2MS基本培养基是硬齿猕猴桃较佳的生根培养基;椰糠+珍珠岩(20∶1)是硬齿猕猴桃较好的培养瓶外生根基质。
In this paper, the tissue culture and rapid propagation techniques of wild species Actinidia callosa Lindl. were studied by using its seeds and stem segments as explants. The results showed that: at the initial culture stage, a certain concentration of 6-BA, ZT and IBA had no promoting effects on the seed germination of A. callosa, the MS basic medium without any hormone was the best medium for the seed germination of A. callosa, and MS+0.5 mg/L 6-BA+0.2 mg/L IBA could be used as the better medium for the stem segment growth of A. callosa; at the subculture stage, MS+2.0 mg/L 6-BA+0.2 mg/L IBA was better conducive to the proliferation of A. callosa; 1/2 MS basic medium without any hormone was the better rooting medium; coconut bran plus perlite(20∶1) was the better rooting substrate for A. callosa plantlets outside the culture bottle.
In this paper, the tissue culture and rapid propagation techniques of wild species Actinidia callosa Lindl. were studied by using its seeds and stem segments as explants. The results showed that: at the initial culture stage, a certain concentration of 6-BA, ZT and IBA had no promoting effects on the seed germination of A. callosa, the MS basic medium without any hormone was the best medium for the seed germination of A. callosa, and MS+0.5 mg/L 6-BA+0.2 mg/L IBA could be used as the better medium for the stem segment growth of A. callosa; at the subculture stage, MS+2.0 mg/L 6-BA+0.2 mg/L IBA was better conducive to the proliferation of A. callosa; 1/2 MS basic medium without any hormone was the better rooting medium; coconut bran plus perlite(20∶1) was the better rooting substrate for A. callosa plantlets outside the culture bottle.
引文
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[4] 阳小成,王伯初,叶志义,等.中华猕猴桃的组织培养及其实用快速繁殖[J].重庆大学学报,2002,25(6):75-77.
[5] 刘永立,兰大伟,毕静华,等.葛枣猕猴桃组织培养中的器官形成与植株再生[J].果树学报,2005,22(3):220-223.
[6] 严姜黎,张翼,邢梅,等.红肉猕猴桃离体快速繁殖技术研究[J].华中农业大学学报,2008,27(1):101-104.
[7] 文国琴,何震.红阳猕猴桃茎段愈伤组织诱导成苗技术[J].福建林业科技,2004,31(4):78-79.
[8] 张远记,钱迎倩.软枣猕猴桃试管苗和茎段的愈伤组织诱导及植株再生[J].西北植物学报,1996,16(2):137-141.
[2] 邹游,黄敏,侯若彤,等.ISSR标记技术在猕猴桃遗传研究中的运用[J].西南师范大学学报,2008,33(1):111-115.
[3] 桂耀林.猕猴桃离体茎段愈伤组织的诱导和植株再生[J].植物生理学通讯,1979,21(4):339-343.
[4] 阳小成,王伯初,叶志义,等.中华猕猴桃的组织培养及其实用快速繁殖[J].重庆大学学报,2002,25(6):75-77.
[5] 刘永立,兰大伟,毕静华,等.葛枣猕猴桃组织培养中的器官形成与植株再生[J].果树学报,2005,22(3):220-223.
[6] 严姜黎,张翼,邢梅,等.红肉猕猴桃离体快速繁殖技术研究[J].华中农业大学学报,2008,27(1):101-104.
[7] 文国琴,何震.红阳猕猴桃茎段愈伤组织诱导成苗技术[J].福建林业科技,2004,31(4):78-79.
[8] 张远记,钱迎倩.软枣猕猴桃试管苗和茎段的愈伤组织诱导及植株再生[J].西北植物学报,1996,16(2):137-141.