海产品油脂中无机砷提取方法的优化研究
|
摘要
目前海产品中测定无机砷的主要方法是原子荧光光谱法,而无机砷的提取是至关重要的一步。本文在GB 5009.11-2014的基础上,综合了水浴和超声波振荡两种提取方法,选择6mol·L~(-1)的盐酸作为浸提液,建立了盐酸浸提-水浴超声提取法,进一步优化了试样的提取方法,以满足海产品中无机砷含量准确测定的需要。试验结果发现,水浴超声提取法是一种新的适用于海洋性动物油脂的无机砷检测的预处理方法,样品的加标回收率和精密度均要高于水浴振荡法。海产品油脂中无机砷的最佳提取条件为:60℃水浴下超声提取1h。
The main method for the determination of inorganic arsenic in seafood is atomic fluorescence spectrometry,and the extraction of inorganic arsenic is a crucial step.In this paper,based on GB 5009.11-2014,two kinds of extraction methods,such as water bath and ultrasonic oscillation,were selected as 6.0 mol·L~(-1) hydrochloric acid as the extracting solution.The extraction method was extracted by hydrochloric acid and ultrasonic oscillation water bath,and the extraction of the sample was further optimized method to meet the need for accurate determination of inorganic arsenic in seafood.The results show that the ultrasonic oscillation water bath method is a new pretreatment method for the detection of inorganic arsenic in seafood oils,and the spiked recovery and precision of the samples are higher than conventional water bath oscillation method.The optimum extraction conditions of inorganic arsenic in seafood oils were as follows:ultrasonic oscillation water bath was carried out at 60 ℃ for 1 hour.
The main method for the determination of inorganic arsenic in seafood is atomic fluorescence spectrometry,and the extraction of inorganic arsenic is a crucial step.In this paper,based on GB 5009.11-2014,two kinds of extraction methods,such as water bath and ultrasonic oscillation,were selected as 6.0 mol·L~(-1) hydrochloric acid as the extracting solution.The extraction method was extracted by hydrochloric acid and ultrasonic oscillation water bath,and the extraction of the sample was further optimized method to meet the need for accurate determination of inorganic arsenic in seafood.The results show that the ultrasonic oscillation water bath method is a new pretreatment method for the detection of inorganic arsenic in seafood oils,and the spiked recovery and precision of the samples are higher than conventional water bath oscillation method.The optimum extraction conditions of inorganic arsenic in seafood oils were as follows:ultrasonic oscillation water bath was carried out at 60 ℃ for 1 hour.
引文
[1]Molin M,Ulven S U,Meltzer H M,et al.Arsenic in the human food chain,biotransformation and toxicologyReview focusing on seafood arenic[J].J Trace Elem Med Bio,2015,31:249-259.
[2]杨俊,张琳琳,黎淑贞,等.氢化物原子荧光光度法测定食品中无机砷含量前处理方法的改进[J].广东化工,2015,42(4):121-122.
[3]高鹭,董伟峰,彭心婷,等.高效液相色谱氢化物发生原子荧光光谱联用检测海藻中砷形态[J].食品安全质量检测学报,2015,(1):145-151.
[4]董喆,高明义,李梦怡,等.高效液相色谱-氢化物发生原子荧光联用法检测不同基质食品中的砷含量及其形态[J].食品安全质量检测学报,2016,7(3):927-932.
[5]Taylor V,Goodale B,Raab A,et al.Human exposure to organic arsenic species from seafood[J].Sci Total Environ,2017,580:266-282.
[6]魏晓丹,徐开来,侯贤灯,等.微波辅助氯化物发生-原子荧光光谱法测定水样中的痕量砷[J].化学研究与应用,2010,22(4):461-464.
[7]丘婷婷,吴钟玲,吴志谊.动物性食品中无机砷的测定[J].广东微量元素科学,2012,19(6):34-37.
[8]宋根林,黄高超,蒋涛,等.原子荧光光度法测定食品中的无机砷方法的改进[J].中国食品工业,2011,(12):52-52.
[9]GB/T 5009.11-2003.食品中总砷及无机砷的测定[S].
[10]GB 5009.11-2014.食品中总砷及无机砷的测定[S].
[11]刘玉兰,张惠娟,向国强,等.植物油料及食用油中砷的研究进展[J].河南农业大学学报:自然科学版,2013,34(1):108-113.
[2]杨俊,张琳琳,黎淑贞,等.氢化物原子荧光光度法测定食品中无机砷含量前处理方法的改进[J].广东化工,2015,42(4):121-122.
[3]高鹭,董伟峰,彭心婷,等.高效液相色谱氢化物发生原子荧光光谱联用检测海藻中砷形态[J].食品安全质量检测学报,2015,(1):145-151.
[4]董喆,高明义,李梦怡,等.高效液相色谱-氢化物发生原子荧光联用法检测不同基质食品中的砷含量及其形态[J].食品安全质量检测学报,2016,7(3):927-932.
[5]Taylor V,Goodale B,Raab A,et al.Human exposure to organic arsenic species from seafood[J].Sci Total Environ,2017,580:266-282.
[6]魏晓丹,徐开来,侯贤灯,等.微波辅助氯化物发生-原子荧光光谱法测定水样中的痕量砷[J].化学研究与应用,2010,22(4):461-464.
[7]丘婷婷,吴钟玲,吴志谊.动物性食品中无机砷的测定[J].广东微量元素科学,2012,19(6):34-37.
[8]宋根林,黄高超,蒋涛,等.原子荧光光度法测定食品中的无机砷方法的改进[J].中国食品工业,2011,(12):52-52.
[9]GB/T 5009.11-2003.食品中总砷及无机砷的测定[S].
[10]GB 5009.11-2014.食品中总砷及无机砷的测定[S].
[11]刘玉兰,张惠娟,向国强,等.植物油料及食用油中砷的研究进展[J].河南农业大学学报:自然科学版,2013,34(1):108-113.