外源亚精胺对等渗盐胁迫下番茄幼苗抗氧化系统的影响
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摘要
【目的】探讨等渗盐胁迫下外源亚精胺(Spd)对番茄幼苗抗氧化系统的影响。【方法】采用水培法,以盐敏感型番茄品种‘中杂9号’为材料,以150mmol/L NaCl和100mmol/L Ca(NO_3)_2模拟等渗盐胁迫环境,设置5个处理,分别为:对照.1/2倍Hoagland营养液+叶面喷水;单独NaCl处理.150mmol/L NaCl溶液+叶面喷水;单独Ca(NO_3)_2处理.100mmol/L Ca(NO_3)_2溶液+叶面喷水;NaCl+Spd处理.150mmol/L NaCl溶液+叶面喷0.25mmol/L Spd;Ca(NO_3)_2+Spd处理.100mmol/L Ca(NO_3)_2溶液+叶面喷0.25mmol/L Spd,研究等渗盐胁迫下外源Spd对番茄幼苗生物量,超氧阴离子自由基(O_2~(-·))、过氧化氢(H_2O_2)、丙二醛(MDA)含量,相对质膜透性及抗氧化酶(SOD、POD、CAT、APX)活性的影响。【结果】与对照相比,等渗NaCl、Ca(NO_3)_2胁迫下,番茄幼苗生物量和SOD、POD、CAT、APX活性均显著降低,相对质膜透性、MDA、O_2~(-·)和H_2O_2含量均显著增加。与单独NaCl或Ca(NO_3)_2胁迫相比,2种盐胁迫下再喷施Spd后明显增加了番茄幼苗生物量和抗氧化酶活性,显著降低了相对质膜透性、MDA、O_2~(-·)和H_2O_2含量,且外源Spd对NaCl胁迫的调控效应更为显著。【结论】外源Spd可通过提高抗氧化酶活性来增强番茄幼苗清除体内活性氧(ROS)及降低膜透性的能力,缓解盐胁迫对番茄幼苗生长的抑制作用,从而提高番茄抗盐性。单独Ca(NO_3)_2胁迫下植物受到的氧化伤害程度低于单独NaCl胁迫。
【Objective】This study aimed to investigate the regulation effects of exogenous spermidine(Spd)on antioxidant system of tomato(Solanum lycopersicum L.)seedlings under isotonic salt stress.【Method】The salt sensitive tomato variety(‘Zhongza 9’)was treatment with isotonic saline stress simulated by 150 mmol/L NaCl and 100 mmol/L Ca(NO_3)_2.Five treatments were set including the control(half-strength Hoagland’s nutrient solution cultivation and sprayed with distilled water),single NaCl treatment(seedlings were exposed to half-strength Hoagland’s nutrient solution containing 150mmol/L saline solution and sprayed with distilled water),single Ca(NO_3)_2treatment(seedlings were exposed to half-strength Hoagland’s nutrient solution containing 100 mmol/L saline solution and sprayed with distilled water),NaCl+Spd treatment(seedlings were exposed to half-strength Hoagland’s nutrient solution containing 150mmol/L saline solution and sprayed with 0.25mmol/L Spd)and Ca(NO_3)_2+Spd treatment(seedlings were exposed to half-strength Hoagland’s nutrient solution containing 100mmol/L saline solution and sprayed with 0.25mmol/L Spd).Then,the effects of foliar-spraying Spd on biomass,contents of superoxide anion radica(O_2~(-·)),hydrogen peroxide(H_2O_2)and malondialdehyde(MDA),relative plasma membrane permeability and activities of antioxidant enzymes(SOD,POD,CAT,and APX)were analyzed.【Result】Compared with the control,the biomass and activities of SOD,POD,CAT and APX of tomato seedlings decreased significantly,while the relative plasma membrane permeability and contents of MDA,O_2~(-·)and H_2O2increased significantly under isotonic NaCl and Ca(NO_3)_2stress.In comparison with the single NaCl treatment or Ca(NO_3)_2treatment,spraying Spd increased the biomass and antioxidant enzyme activities of tomato seedlings significantly but reduced the relative plasma membrane permeability and contents of MDA,O_2~(-·)and H_2O_2significantly.The effect of exogenous Spd on regulation of NaCl stress was more significant.【Conclusion】Exogenous Spd could improve antioxidant enzyme activities to enhance the scavenging ability of reactive oxygen species(ROS)and decrease membrane permeability of tomato seedlings.Thus,the inhibitory of salt stress on growth was alleviated and the salt resistance of tomato seedlings was improved.The oxidative damage degree of plants under single Ca(NO_3)_2stress was lower than under single NaCl stress.
【Objective】This study aimed to investigate the regulation effects of exogenous spermidine(Spd)on antioxidant system of tomato(Solanum lycopersicum L.)seedlings under isotonic salt stress.【Method】The salt sensitive tomato variety(‘Zhongza 9’)was treatment with isotonic saline stress simulated by 150 mmol/L NaCl and 100 mmol/L Ca(NO_3)_2.Five treatments were set including the control(half-strength Hoagland’s nutrient solution cultivation and sprayed with distilled water),single NaCl treatment(seedlings were exposed to half-strength Hoagland’s nutrient solution containing 150mmol/L saline solution and sprayed with distilled water),single Ca(NO_3)_2treatment(seedlings were exposed to half-strength Hoagland’s nutrient solution containing 100 mmol/L saline solution and sprayed with distilled water),NaCl+Spd treatment(seedlings were exposed to half-strength Hoagland’s nutrient solution containing 150mmol/L saline solution and sprayed with 0.25mmol/L Spd)and Ca(NO_3)_2+Spd treatment(seedlings were exposed to half-strength Hoagland’s nutrient solution containing 100mmol/L saline solution and sprayed with 0.25mmol/L Spd).Then,the effects of foliar-spraying Spd on biomass,contents of superoxide anion radica(O_2~(-·)),hydrogen peroxide(H_2O_2)and malondialdehyde(MDA),relative plasma membrane permeability and activities of antioxidant enzymes(SOD,POD,CAT,and APX)were analyzed.【Result】Compared with the control,the biomass and activities of SOD,POD,CAT and APX of tomato seedlings decreased significantly,while the relative plasma membrane permeability and contents of MDA,O_2~(-·)and H_2O2increased significantly under isotonic NaCl and Ca(NO_3)_2stress.In comparison with the single NaCl treatment or Ca(NO_3)_2treatment,spraying Spd increased the biomass and antioxidant enzyme activities of tomato seedlings significantly but reduced the relative plasma membrane permeability and contents of MDA,O_2~(-·)and H_2O_2significantly.The effect of exogenous Spd on regulation of NaCl stress was more significant.【Conclusion】Exogenous Spd could improve antioxidant enzyme activities to enhance the scavenging ability of reactive oxygen species(ROS)and decrease membrane permeability of tomato seedlings.Thus,the inhibitory of salt stress on growth was alleviated and the salt resistance of tomato seedlings was improved.The oxidative damage degree of plants under single Ca(NO_3)_2stress was lower than under single NaCl stress.
引文
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[2]周珩,郭世荣,邵慧娟,等.等渗NaCl和Ca(NO3)2胁迫对黄瓜幼苗生长和生理特性的影响[J].生态学报,2014,34(7):1880-1890.Zhou H,Guo S R,Shao H J,et al.Effects of iso-osmotic Ca(NO3)2and NaCl stress on growth and physiological characteristics of cucumber seedlings[J].Acta Ecologica Sinica,2014,34(7):1880-1890.
[3]何明明,王秀峰,谷端银,等.硝普钠对缺铁和硝酸盐胁迫下番茄幼苗生长及生理特性的影响[J].应用生态学报,2017,28(4):1246-1254.He M M,Wang X F,Gu D Y,et al.Effects of sodium nitroprusside on growth and physiological characteristics of tomato seedlings under iron deficiency and NO3-stress[J].Chinese Journal of Applied Ecology,2017,28(4):1246-1254.
[4]周静,徐强,张婷.NaCl胁迫对不同品种辣椒幼苗生理生化特性的影响[J].西北农林科技大学学报(自然科学版),2015,43(2):120-125.Zhou J,Xu Q,Zhang T.Effect of NaCl stress on physiological characteristics of seedlings of different pepper varieties[J].Journal of Northwest A&F University(Nat Sci Ed),2015,43(2):120-125.
[5]王素平,郭世荣,胡晓辉,等.NaCl胁迫对黄瓜幼苗植株生长和光合特性的影响[J].西北植物学报,2006,26(3):455-461.Wang S P,Guo S R,Hu X H,et al.Effects of NaCl stress on growth and photosynthetic characteristics of cucumber(Cucumber sativus L.)seedlings[J].Acta Botanica Boreali-Occidentalia Sinica,2006,26(3):455-461.
[6]何勇,朱祝军.等渗的Ca(NO3)2和NaCl胁迫对番茄根系呼吸和活性氧代谢的影响[J].浙江大学学报,2013,39(4):396-402.He Y,Zhu Z J.Effects of iso-osmotic Ca(NO3)2 and NaCl stress on respiration and metabolism of reactive oxygen species in roots of tomato seedlings[J].Journal of Zhejiang University,2013,39(4):396-402.
[7]梁杨杨,吴晓蕾,李敬蕊,等.盐碱胁迫下GABA对番茄幼苗活性氧代谢及光合性能的影响[J].河北农业大学学报,2016,39(3):7-13.Liang Y Y,Wu X L,Li J R,et al.Effects of exogenous GABAon reactive oxygen species metabolism and photosynthesis characteristics of tomatoes under salinity-alkalinity stress[J].Journal of Hebei Agricultural University,2016,39(3):7-13.
[8]Groppa M D,Benavides M P.Polyamines and abiotic stress:recent advances[J].Amino Acids,2008,34:35-45.
[9]Hu X H,Zhang Y,Shi Y,et al.Effect of exogenous spermidine on polyamine content and metabolism in tomato exposed to salinity-alkalinity mixed stress[J].Plant Physiology and Biochemistry,2012,57:200-209.
[10]Kasukabe Y,He L,Nada K,et al.Overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and upregulates the expression of various stress-regulated genes in transgenic Arabidopsis thaliana[J].Plant and Cell Physiology,2004,45(6):712-722.
[11]Kasukabe Y,He L X,Watakabe Y,et al.Improvement of environmental stress tolerance of sweet potato by introduction of genes for spermidine synthase[J].Plant Biotechnology,2006,23(1):75-83.
[12]田婧,郭世荣,孙锦,等.外源Spd对高温胁迫下黄瓜幼苗叶片膜脂过氧化及质子泵活性的影响[J].应用生态学报,2011,22(12):3252-3258.Tian J,Guo S R,Sun J,et al.Effects of exogenous spermidine on lipid peroxidation and membrane proton pump activity of cucumber seedling leaves under high temperature stress[J].Chinese Journal of Applied Ecology,2011,22(12):3252-3258.
[13]张永平,许爽,杨少军,等.外源亚精胺对低温胁迫下甜瓜幼苗生长和抗氧化系统的影响[J].植物生理学报,2017,53(6):1087-1096.Zhang Y P,Xu S,Yang S J,et al.Effect of exogenous spermidine on the growth and antioxidant system of melon seedlings under low temperature stress[J].Plant Physiology Journal,2017,53(6):1087-1096.
[14]张春梅,邹志荣,张志新,等.外源亚精胺对模拟干旱胁迫下番茄幼苗活性氧水平和抗氧化系统的影响[J].应用与环境生物学报,2009,15(3):301-307.Zhang C M,Zou Z R,Zhang Z X,et al.Effects of exogenous spermidine on reactive oxygen levels and antioxidative system of tomato seedling under polyethlene glycol stress[J].Journal of Application and Environmental Biology,2009,15(3):301-307.
[15]李娜,陈红,裴孝伯.外源亚精胺对盐胁迫下黄瓜幼苗耐盐性的影响[J].热带作物学报,2013,34(7):1359-1364.Li N,Chen H,Pei X B.Effects of extraneous spermidine on the salt tolerance in cucumber seedlings under salt stress[J].Chinese Journal of Tropical Crops,2013,34(7):1359-1364.
[16]汪志伟,贠文俊,颉建明,等.外源亚精胺对盐胁迫下辣椒幼苗生长抑制的缓解效应[J].甘肃农业大学学报,2009,44(4):67-72.Wang Z W,Yun W J,Xie J M,et al.Mitigative effect of exogenous spermidine on growth inhibition in pepper seedlings under NaCl stress[J].Journal of Gansu Agricultural University,2009,44(4):67-72.
[17]周晨楠,施晓梦,袁颖辉,等.外源亚精胺对Ca(NO3)2胁迫下番茄幼苗光合特性和抗氧化酶活性的影响[J].西北植物学报,2012,32(3):498-504.Zhou C N,Shi X M,Yuan Y H,et al.Effects of exogenous spermidine on photosynthetic characteristics and active antioxidant enzyme activity in tomato seedlings under Ca(NO3)2stress[J].Acta Botanica Boreali-Occidentalia Sinica,2012,32(3):498-504.
[18]Cao W H,Liu J,He X J,et al.Modulation of ethylene responses affects plant salt-stress responses[J].Plant Physiol,2007,143(2):707-719.
[19]Bailly C,Benamar A,Corbineau F,et al.Changes in malondialdehyde content and in superoxide dismutase,catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging[J].Physiol Plant,1996,97:104-110.
[20]王爱国,罗广华.植物的超氧物自由基与羟胺反应的定量关系[J].植物生理学通讯,1990,26(6):55-57.Wang A G,Luo G H.Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants[J].Plant Physiology Communications,1990,26(6):55-57.
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