超高效液相色谱法测定绿茶中花青素的含量
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摘要
本研究建立了绿茶中花青素的超高效液相色谱分析方法。样品经过乙醇盐酸水溶液提取,沸水浴将花色苷酸水解为花青素单体。采用BEH C18柱(2.1 mm×100 mm,1.7μm)进行分离,二极管陈列检测器检测,外标法定量。结果表明,6种花青素线性相关系数为0.999 6～0.999 9,回收率为78.5%～95.9%,相对标准偏差为1.5%～8.2%。该方法快速准确,灵敏度高、重现性好,适用于绿茶中花青素单体的定性和定量检测。
In this research, a ultra performance liquid chromatographic method was estabolished for the quantification of anthocyanidins in green tea. After extracted under the ultrasonic irradiation in HCl aqueous solution,the anthocyanins were directly hydrolyzed into anthocyanidins. The separation column was Acquity UPLC BEH C18(100 mm×2.1 mm,1.7 μm),the sample was detected by PDA detector and quantitative analysis by external standard method. The result showed that the correlation coefficients were 0.999 6～0.999 9. The average recoveries were in the range of 78.5%～95.9%. The relative standard deviations were between 1.5% and 8.2%. The method was simple,sensitive and accurate for the determination of anthocyanidins content in green tea.
In this research, a ultra performance liquid chromatographic method was estabolished for the quantification of anthocyanidins in green tea. After extracted under the ultrasonic irradiation in HCl aqueous solution,the anthocyanins were directly hydrolyzed into anthocyanidins. The separation column was Acquity UPLC BEH C18(100 mm×2.1 mm,1.7 μm),the sample was detected by PDA detector and quantitative analysis by external standard method. The result showed that the correlation coefficients were 0.999 6～0.999 9. The average recoveries were in the range of 78.5%～95.9%. The relative standard deviations were between 1.5% and 8.2%. The method was simple,sensitive and accurate for the determination of anthocyanidins content in green tea.
引文
[1]赵云荣,王世雷.植物花青素研究进展[J].安徽农业科学,2008,36(8):3095-3097.
[2]刘林峰,向奕,刘安,等.茶叶花青素的研究进展[J].茶叶通讯,2018,45(1):3-8.
[3]TERAHARA N,TAKEDA Y,NESUMI A,et al.Anthocyanins from red flower tea(Benibana-cha),Camellia sinensis[J].Phytochemistry,2001(4):359-361.
[4]王秋霜,凌彩金,刘淑媚,等.红紫芽茶叶花青素提取分离及p H稳定性初探[J].中国农学通报,2014,30(9):291-296.
[5]赵尔丰,高畅,高欣,等.酶-超声波辅助提取蓝莓果渣中花青素的工艺研究[J].东北农业大学学报,2010,41(4):98-103.
[6]孙建霞,张燕,孙志健,等.花色苷的资源分布以及定性定量分析方法研究进展[J].食品科学,2009,30(5):263-268.
[7]刘玉芹,王晓,杜金华.花色苷的分离纯化及定性定量方法研究进展[J].中国食品添加剂,2010(6):178-182.
[8]于晓南.植物叶片中花青素的分析与研究[J].现代仪器,2000(4):37-38.
[9]李智,王日为,张丽霞,等.茶树紫色芽叶花青苷组分分析及结构推测[J].茶叶科学,2014(3):279-287.
[10]NY/T 2640-2014植物源性食品中花青素的测定高效液相色谱法[S].
[2]刘林峰,向奕,刘安,等.茶叶花青素的研究进展[J].茶叶通讯,2018,45(1):3-8.
[3]TERAHARA N,TAKEDA Y,NESUMI A,et al.Anthocyanins from red flower tea(Benibana-cha),Camellia sinensis[J].Phytochemistry,2001(4):359-361.
[4]王秋霜,凌彩金,刘淑媚,等.红紫芽茶叶花青素提取分离及p H稳定性初探[J].中国农学通报,2014,30(9):291-296.
[5]赵尔丰,高畅,高欣,等.酶-超声波辅助提取蓝莓果渣中花青素的工艺研究[J].东北农业大学学报,2010,41(4):98-103.
[6]孙建霞,张燕,孙志健,等.花色苷的资源分布以及定性定量分析方法研究进展[J].食品科学,2009,30(5):263-268.
[7]刘玉芹,王晓,杜金华.花色苷的分离纯化及定性定量方法研究进展[J].中国食品添加剂,2010(6):178-182.
[8]于晓南.植物叶片中花青素的分析与研究[J].现代仪器,2000(4):37-38.
[9]李智,王日为,张丽霞,等.茶树紫色芽叶花青苷组分分析及结构推测[J].茶叶科学,2014(3):279-287.
[10]NY/T 2640-2014植物源性食品中花青素的测定高效液相色谱法[S].