BS69在结直肠癌组织中的表达以及病理相关性研究
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摘要
目的:探讨BS69在结直肠癌组织及细胞系中蛋白和mRNA的表达水平,分析BS69与患者病理特征的相关性。方法:选择2016年8月至2017年10月间手术切除的60例结直肠癌组织和25例癌旁正常组织,采用免疫组化和RTq PCR法检测组织中BS69蛋白和mRNA的表达情况。采用蛋白印迹(Western blot)法检测BS69在三种结直肠癌细胞系和正常结直肠上皮细胞中的蛋白表达。结果:BS69免疫组化染色主要定位于细胞核,少量位于细胞浆。其在结直肠癌和正常结直肠组织中的阳性率分别为65%和87%,差异具有统计学意义(P<0. 05); BS69在结直肠癌组织中的表达水平与Dukes分期、肿瘤组织分级、淋巴结转移及远处转移有相关性(P<0. 05); Western blot检测BS69在3种结直肠癌细胞系与正常结直肠上皮细胞中蛋白表达,各细胞系中结果有明显差异;结直肠癌组织中BS69 mRNA的表达明显低于正常组织,差异有统计学意义(P<0. 05)。结论:BS69是重要的转录抑制因子,可能参与了结直肠癌的发生、发展过程,有望成为特异性较高的肿瘤标志物。
Objective: To investigate the expression levels of protein and mRNA of BS69 in colorectal cancer tissues and cell lines,and analyze the correlation between BS69 and pathological characteristics of patients. Methods: 60 colorectal cancer tissues and25 adjacent normal tissues were resected from August 2016 to October 2017. Immunohistochemistry and RT-qPCR were used to detect the expression of BS69 protein and BS69 mRNA in tissues. The protein expression of BS69 in three colorectal cancer cell lines and normal colorectal epithelial cells was detected by Western blot. Results: Immunohistochemical staining of BS69 was mainly located in the nucleus and a small amount in cytoplasm. The positive rates in colorectal cancer and normal colorectal tissues were 65% and 87%,respectively,with significant difference( P<0. 05). The expression level of BS69 in colorectal cancer was correlated with dukes stage,tumor tissue grade,lymph node metastasis and distant metastasis( P<0. 05). The protein expression of BS69 in 3 colorectal cancer cell lines and normal colorectal epithelial cells was detected by Western blot,the results were significantly different. The expression of BS69 mRNA in colorectal cancer tissues was significantly lower than that in normal tissues,the difference was statistically significant( P <0. 05). Conclusion: BS69 is an important transcriptional inhibitor,which may be involved in the occurrence and development of colorectal cancer. BS69 is expected to become a highly specific tumor marker.
Objective: To investigate the expression levels of protein and mRNA of BS69 in colorectal cancer tissues and cell lines,and analyze the correlation between BS69 and pathological characteristics of patients. Methods: 60 colorectal cancer tissues and25 adjacent normal tissues were resected from August 2016 to October 2017. Immunohistochemistry and RT-qPCR were used to detect the expression of BS69 protein and BS69 mRNA in tissues. The protein expression of BS69 in three colorectal cancer cell lines and normal colorectal epithelial cells was detected by Western blot. Results: Immunohistochemical staining of BS69 was mainly located in the nucleus and a small amount in cytoplasm. The positive rates in colorectal cancer and normal colorectal tissues were 65% and 87%,respectively,with significant difference( P<0. 05). The expression level of BS69 in colorectal cancer was correlated with dukes stage,tumor tissue grade,lymph node metastasis and distant metastasis( P<0. 05). The protein expression of BS69 in 3 colorectal cancer cell lines and normal colorectal epithelial cells was detected by Western blot,the results were significantly different. The expression of BS69 mRNA in colorectal cancer tissues was significantly lower than that in normal tissues,the difference was statistically significant( P <0. 05). Conclusion: BS69 is an important transcriptional inhibitor,which may be involved in the occurrence and development of colorectal cancer. BS69 is expected to become a highly specific tumor marker.
引文
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[9]梁立,刘天舒.结直肠癌肝转移危险及预后因素分析[J].中国实用外科杂志,2016,36(4):383-386.Liang L,Liu TS.Risk and prognostic factors of colorectal liver metastases[J].Chin J Pract Surg,2016,36(4):383-386.
[10]Hateboer G,Gennissen A,Ramos YF,et al.BS69,a novel adenovirus E1A-associated protein that inhibits E1Atransactivation[J].Embo J,1995,14(13):3159-3169.
[11]Zhang W,Chan HM,Gao Y et al.BS69 is involved in cellular senescence through the p53-p21Cip1 pathway[J].Embo J,2007,8(10):952-958.
[12]秦苏.人BRPF2和BS69蛋白的几个结构域的结构与功能研究[D].中国科学技术大学,2011:87-90.Su Q.Structural and Functional Study of human BRPF2 and BS69[D].University of Science and Technology of China,2011:87-90.
[2]Sung JJ,Lau JY,Goh KL,et al.Increasing incidence of colorectal cancer in Asia:implications for screening[J].Lancet Oncology,2005,6(11):871-876.
[3]Jemal A,Bray F,Center MM,et al.Global cancer statistics[J].Ca Cancer J Clinicians,2015,61(2):69-90.
[4]Ekblad CM,Chavali GB,Basu BP,et al.Binding of EMSY to HP1beta:implications for recruitment of HP1beta and BS69[J].Embo J,2005,6(7):675-680.
[5]Wan J,Zhang W,Wu L,et al.BS69,a specific adaptor in the latent membrane protein 1-mediated c-Jun N-terminal kinase pathway[J].Mol Cell Biol,2006,26(2):448-456.
[6]钟华,刘迪群.结直肠癌组织中Ki-67和COX-2的表达及其临床意义[J].吉林大学学报,2016,42(6):1168-1172.Zhong H,Liu DQ.Expressions of COX-2 and Ki-67 in colorectal carcinoma tissue and their clinical significances[J].J Jilin Univ,2016,42(6):1168-1172.
[7]Song M,Garrett WS,Chan AT.Nutrients,foods,and colorectal cancer prevention[J].Gastroenterol,2015,148(6):1244-1260.
[8]于晓强,何和平,沈泽旭,等.腹腔镜手术与传统开腹手术在结直肠癌根治术中的临床疗效比较[J].西部医学,2015,27(6):911-913.Yu XQ,He HP,Shen ZX.Comparison of the clinical efficacy of laparoscopic colorectal cancer radical operation and traditional open operation[J].Med J West China,2015,27(6):911-913.
[9]梁立,刘天舒.结直肠癌肝转移危险及预后因素分析[J].中国实用外科杂志,2016,36(4):383-386.Liang L,Liu TS.Risk and prognostic factors of colorectal liver metastases[J].Chin J Pract Surg,2016,36(4):383-386.
[10]Hateboer G,Gennissen A,Ramos YF,et al.BS69,a novel adenovirus E1A-associated protein that inhibits E1Atransactivation[J].Embo J,1995,14(13):3159-3169.
[11]Zhang W,Chan HM,Gao Y et al.BS69 is involved in cellular senescence through the p53-p21Cip1 pathway[J].Embo J,2007,8(10):952-958.
[12]秦苏.人BRPF2和BS69蛋白的几个结构域的结构与功能研究[D].中国科学技术大学,2011:87-90.Su Q.Structural and Functional Study of human BRPF2 and BS69[D].University of Science and Technology of China,2011:87-90.