辛复宁对HPV感染相关的宫颈疾病疗效及对免疫的影响
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摘要
目的:探讨辛复宁治疗人乳头瘤病毒(HPV)感染相关的宫颈疾病效果及其对免疫功能影响。方法:选取2016年5月—2017年5月本院收治的HPV感染患者90例,随机数字表法分为对照组和观察组各45例,对照组采用常规治疗,观察组在对照组基础上采用辛复宁治疗,观察两组治疗前后1个月机体T淋巴细胞亚群、免疫球蛋白和炎症因子变化,临床疗效及6个月后HPV感染复发情况。结果:宫颈局部CD3~+ T细胞、CD8~+ T细胞、CD4~+ CD25~+ T细胞以及Foxp3~+ Tregs、IgA、IgG和IgM、C-反应蛋白(CRP)、肿瘤坏死因子-(TNF-)、干扰素(IFN-)和白细胞介素-6(IL-6)等水平,治疗前两组无统计学差异(P>0.05),治疗后两组均有改善,且观察组改善程度优于对照组(P<0.05);治疗6个月后观察组HPV感染复发率(2.2%)低于对照组(15.6%)(P<0.05)。结论:辛复宁可治疗HPV感染相关的宫颈疾病疗效显著,感染复发更低,与患者机体T淋巴细胞亚群、免疫球蛋白和炎症因子等水平改善有关。
Objective: To investigate the therapeutic effect of Xinfuning on cervical diseases by human papillomavirus(HPV) infected,and to study its effect on immunity of patients. Methods: 90 women with HPV infection-related cervical diseases were divided into the control group and study group from May 2016 to May 2017. The women in control group were treated by conventional therapy, and the women in study group were treated by Xinfuning except conventional therapy. The situation of T lymphocyte subsets, and the change of immunoglobulin and inflammatory cytokines of women in the two groups before and 1 month after treatment were recorded and compared, and the clinical efficacy and recurrence of HPV infected of women in the two groups after 6 months were also recorded. Results: Before treatment, the levels of CD3~+ T cells, CD8~+ T cells, CD4~+ CD25~+ T cells, Foxp3~+ Tregs, IgA, IgG and IgM, C-reactive protein(CRP), tumor necrosis factor alpha(TNF-a), interferon(IFN) and interleukin-6(IL-6) of women in both groups had no significant different(P>0.05). After treatment, all above indexes of women in both groups had improved, and the changes of all above indexes of women in control group were significant better than those of women in control group(P<0.05). 6 months after treatment, the incidence of HPV infected again of women in control group was significant lower than that of women in control group(P<0.05). Conclusion: Xinfuning can be used to treat HPV infection-related cervical diseases, which can decrease rate of HPV infected again of women by improving the levels of T lymphocyte subsets, immunoglobulin, and inflammatory cytokines.
Objective: To investigate the therapeutic effect of Xinfuning on cervical diseases by human papillomavirus(HPV) infected,and to study its effect on immunity of patients. Methods: 90 women with HPV infection-related cervical diseases were divided into the control group and study group from May 2016 to May 2017. The women in control group were treated by conventional therapy, and the women in study group were treated by Xinfuning except conventional therapy. The situation of T lymphocyte subsets, and the change of immunoglobulin and inflammatory cytokines of women in the two groups before and 1 month after treatment were recorded and compared, and the clinical efficacy and recurrence of HPV infected of women in the two groups after 6 months were also recorded. Results: Before treatment, the levels of CD3~+ T cells, CD8~+ T cells, CD4~+ CD25~+ T cells, Foxp3~+ Tregs, IgA, IgG and IgM, C-reactive protein(CRP), tumor necrosis factor alpha(TNF-a), interferon(IFN) and interleukin-6(IL-6) of women in both groups had no significant different(P>0.05). After treatment, all above indexes of women in both groups had improved, and the changes of all above indexes of women in control group were significant better than those of women in control group(P<0.05). 6 months after treatment, the incidence of HPV infected again of women in control group was significant lower than that of women in control group(P<0.05). Conclusion: Xinfuning can be used to treat HPV infection-related cervical diseases, which can decrease rate of HPV infected again of women by improving the levels of T lymphocyte subsets, immunoglobulin, and inflammatory cytokines.
引文
[1] Katki HA, Schiffman M.A novel metric that quantifies risk stratification for evaluating diagnostic tests: The example of evaluating cervical-cancer screening tests across populations[J].Prev Med,2018,14(2);110:100-105.
[2] Qian HZ, Hu Y, Carlucci JG, et al.Human Immunodeficiency Virus Status Differentially Associated With Genital and Anal Human Papillomavirus Infection Among Chinese Men Who Have Sex With Men: A Cross-Sectional Survey[J].Sex Transm Dis,2017,44(11):656-662.
[3] Zhou HL, Zhang W, Zhang CJ,et al.Prevalence and distribution of human papillomavirus genotypes in Chinese women between 1991 and 2016: A systematic review[J]. J infect,2018 Mar 1. pii: S0163-4453(18)30061-6.
[4] Kikuchi I, Kase S, Ishijima K, et al.Long-term follow-up of conjunctival melanoma treated with topical interferon alpha-2b eye drops as adjunctive therapy following surgical resection[J].Graefes Arch Clin Exp Ophthalmol,2017 ,255(11):2271-2276.
[5] Caglar GS, Garrido N.The implications of male human papilloma virus infection in couples seeking assisted reproduction technologies[J].J Turk Ger Gynecol Assoc,2018,19(1):48-52.
[6] Attarian S, Sharma J, Kaubisch A, et al.Multiple Endocrine and Neoplastic Diseases After Unsuccessful Treatment of Hepatitis C With Interferon and Ribavirin[J].Am J Med Sci,2017,354(1):66.
[7] Jraholmen MW, Basnet P, Acharya G, et al.PEGylated liposomes for topical vaginal therapy improve delivery of interferon alpha[J].Eur J Pharm Biopharm,2017 ,113(4):132-139.
[8] Zhao HD, Feng XL, Zhao Y, et al.Randomized controlled study: Sophora flavescens gel in treatment of cervical HPV infection[J].Zhongguo Zhong Yao Za Zhi,2016,41(21):4072-4075.
[9] Woodham AW, Yan L, Skeate JG,et al.T cell ignorance is bliss: T cells are not tolerized by Langerhans cells presenting human papillomavirus antigens in the absence of costimulation[J].Papillomavirus Res,2016,2(12):21-30.
[10] Singh M, Thakral D, Rishi N,et al.Functional characterization of CD4 and CD8 T cell responses among human papillomavirus infected patients with ano-genital warts[J].Virusdisease,2017,28(2):133-140.
[11] Punt S, Dronkers EA, Welters MJ,A beneficial tumor microenvironment in oropharyngeal squamous cell carcinoma is characterized by a high T cell and low IL-17(+) cell frequency[J].Cancer Immunol Immunother,2016,65(4):393-403.
[12] Zhao J, Zeng W, Cao Y,Immunotherapy of HPV infection-caused genital warts using low dose cyclophosphamide[J].Expert Rev Clin Immunol,2014,10(6):791-799.
[13] Lam JO, Bream JH, Sugar EA, et al. Association of serum cytokines with oral HPV clearance[J].Cytokine,2016,83(7):85-91.
[14] 张树丹,李琪,李戈.重组人干扰素α2b阴道泡腾胶囊治疗HPV感染的Meta分析[J].中国医药导报,2017,14(31):111-115.
[15] 刘冬梅.重组人干扰素α-2b凝胶治疗慢性宫颈炎合并高危型HPV感染的临床疗效[J].中外女性健康研究,2017(20):96-97.
[16] 马小磊,张雪艳,范蓉.重组人干扰素α2b阴道泡腾胶囊联合宫颈电切术治疗宫颈上皮内瘤样病变伴高危型HPV感染的临床效果[J].中国妇幼保健,2017,32(09):2011-2014.
[17] 金镇英.重组人干扰素α-2b阴道泡腾胶囊治疗宫颈HPV感染效果观察[J].基层医学论坛,2017,21(14):1805-1806.
[2] Qian HZ, Hu Y, Carlucci JG, et al.Human Immunodeficiency Virus Status Differentially Associated With Genital and Anal Human Papillomavirus Infection Among Chinese Men Who Have Sex With Men: A Cross-Sectional Survey[J].Sex Transm Dis,2017,44(11):656-662.
[3] Zhou HL, Zhang W, Zhang CJ,et al.Prevalence and distribution of human papillomavirus genotypes in Chinese women between 1991 and 2016: A systematic review[J]. J infect,2018 Mar 1. pii: S0163-4453(18)30061-6.
[4] Kikuchi I, Kase S, Ishijima K, et al.Long-term follow-up of conjunctival melanoma treated with topical interferon alpha-2b eye drops as adjunctive therapy following surgical resection[J].Graefes Arch Clin Exp Ophthalmol,2017 ,255(11):2271-2276.
[5] Caglar GS, Garrido N.The implications of male human papilloma virus infection in couples seeking assisted reproduction technologies[J].J Turk Ger Gynecol Assoc,2018,19(1):48-52.
[6] Attarian S, Sharma J, Kaubisch A, et al.Multiple Endocrine and Neoplastic Diseases After Unsuccessful Treatment of Hepatitis C With Interferon and Ribavirin[J].Am J Med Sci,2017,354(1):66.
[7] Jraholmen MW, Basnet P, Acharya G, et al.PEGylated liposomes for topical vaginal therapy improve delivery of interferon alpha[J].Eur J Pharm Biopharm,2017 ,113(4):132-139.
[8] Zhao HD, Feng XL, Zhao Y, et al.Randomized controlled study: Sophora flavescens gel in treatment of cervical HPV infection[J].Zhongguo Zhong Yao Za Zhi,2016,41(21):4072-4075.
[9] Woodham AW, Yan L, Skeate JG,et al.T cell ignorance is bliss: T cells are not tolerized by Langerhans cells presenting human papillomavirus antigens in the absence of costimulation[J].Papillomavirus Res,2016,2(12):21-30.
[10] Singh M, Thakral D, Rishi N,et al.Functional characterization of CD4 and CD8 T cell responses among human papillomavirus infected patients with ano-genital warts[J].Virusdisease,2017,28(2):133-140.
[11] Punt S, Dronkers EA, Welters MJ,A beneficial tumor microenvironment in oropharyngeal squamous cell carcinoma is characterized by a high T cell and low IL-17(+) cell frequency[J].Cancer Immunol Immunother,2016,65(4):393-403.
[12] Zhao J, Zeng W, Cao Y,Immunotherapy of HPV infection-caused genital warts using low dose cyclophosphamide[J].Expert Rev Clin Immunol,2014,10(6):791-799.
[13] Lam JO, Bream JH, Sugar EA, et al. Association of serum cytokines with oral HPV clearance[J].Cytokine,2016,83(7):85-91.
[14] 张树丹,李琪,李戈.重组人干扰素α2b阴道泡腾胶囊治疗HPV感染的Meta分析[J].中国医药导报,2017,14(31):111-115.
[15] 刘冬梅.重组人干扰素α-2b凝胶治疗慢性宫颈炎合并高危型HPV感染的临床疗效[J].中外女性健康研究,2017(20):96-97.
[16] 马小磊,张雪艳,范蓉.重组人干扰素α2b阴道泡腾胶囊联合宫颈电切术治疗宫颈上皮内瘤样病变伴高危型HPV感染的临床效果[J].中国妇幼保健,2017,32(09):2011-2014.
[17] 金镇英.重组人干扰素α-2b阴道泡腾胶囊治疗宫颈HPV感染效果观察[J].基层医学论坛,2017,21(14):1805-1806.