东北刺人参不定根对LPS诱导小鼠腹腔巨噬细胞促炎介质的影响
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摘要
东北刺人参是五加科兼具观赏和药用价值的珍贵濒危植物。为了促进东北刺人参产品的开发,在抗炎相关产品生产中以不定根代替短缺的野生植株作为植物材料。该研究利用诱导子未处理和处理的生物反应器培养的东北刺人参不定根水提物处理小鼠腹腔巨噬细胞,通过检测巨噬细胞中一氧化氮(NO)和肿瘤坏死因子-α(TNF-α)及白细胞介素-1β(IL-1β)3种促炎介质的水平,探明不定根的抗炎活性。研究表明:2种东北刺人参不定根提取物中总酚和总黄酮含量有很大差异,诱导子处理的不定根水提物(E 2)中总酚含量是诱导子未处理不定根水提物(E 1)的3.2倍,而总黄酮含量是2.7倍。2种不定根提取物对NO和TNF-α及IL-1β的影响不同,E2对3种促炎介质均有显著的抑制作用,但E1只抑制NO的生成,对TNF-α及IL-1β没有影响,E2的抗炎效果高于E1。因此,今后在进行抗炎相关产品生产中,可利用在生物反应器培养中经诱导子处理的东北刺人参不定根作为原材料。
Oplopanax elatus Nakai is a plant of Araliaceae with ornamental and medicinal values.To promote the product development of O.elatus,the adventitious roots replaced the field grown plants in the anti-inflammatory production.In the present study,the water extracts of O.elatus adventitious roots,which cultured with the untreated bioreactor(E1)and the treated bioreactor with an elicitor(E2),was used to treat the peritoneal macrophages of the mice.The anti-inflammatory activity was investigated by measuring the levels of NO,TNF-α,and IL-1β.In the water extracts of the E1 group and the E2 group,the total phenol and total flavonoid contents was different.The total phenol and the flavonoid contents in E2 group were 3.2 fold and 2.7 fold more than that in the E1 group.The effect of the both extracts on the production of NO,TNF-α,and IL-1β was also different,three pro-inflammatory mediators were all inhib-ited by the E2 group,while only NO was inhibited by the E1 group;the anti-inflammatory activity of the E2 group was higher than that of the E1 group.Consequently,bioreactor cultured adventitious roots treated with elicitor can be used in the anti-inflammatory production of O.elatus in the future.
Oplopanax elatus Nakai is a plant of Araliaceae with ornamental and medicinal values.To promote the product development of O.elatus,the adventitious roots replaced the field grown plants in the anti-inflammatory production.In the present study,the water extracts of O.elatus adventitious roots,which cultured with the untreated bioreactor(E1)and the treated bioreactor with an elicitor(E2),was used to treat the peritoneal macrophages of the mice.The anti-inflammatory activity was investigated by measuring the levels of NO,TNF-α,and IL-1β.In the water extracts of the E1 group and the E2 group,the total phenol and total flavonoid contents was different.The total phenol and the flavonoid contents in E2 group were 3.2 fold and 2.7 fold more than that in the E1 group.The effect of the both extracts on the production of NO,TNF-α,and IL-1β was also different,three pro-inflammatory mediators were all inhib-ited by the E2 group,while only NO was inhibited by the E1 group;the anti-inflammatory activity of the E2 group was higher than that of the E1 group.Consequently,bioreactor cultured adventitious roots treated with elicitor can be used in the anti-inflammatory production of O.elatus in the future.
引文
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[15]管桐,李贺,王洪秋,等.茉莉酸甲酯对生物反应器培养高山红景天愈伤组织有效物质积累的影响[J].延边大学农学学报,2017,39(01):24-28,34.
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[18]苏力德,代那音台,乌恩齐,等.蒙药荠菜总黄酮的提取及其抗炎作用研究[J].内蒙古民族大学学报(自然科学版),2016,31(01):64-67.
[19]杨建华,孟新源,胡君萍,等.沙枣总酚抗炎作用研究[J].中国实验方剂学杂志,2013,19(18):263-266.
[2]刘迪,刘继生,沈海龙,等.东北刺人参研究现状与展望[J].延边大学农学学报,2012,34(2):177-180.
[3]Kim J H,Eom S H,Lee H S,et al.Assessment on antioxidant properties of Oplopanax elatus Nakai in vitro[J].Kor J Med Crop Sci,2007,15:112-119.
[4]Liu P P,Qu Y,Dou D Q,et al.Determination of anticancer constituents in Oplopanax horridus and Oplopanax elatus[J].J Chem Soc Pak,2012,34:417-423.
[5]王广树,杨晓虹,徐景达.东北刺人参叶中四种新三萜皂苷的分离与结构鉴定[J].药学学报,2004,39(5):354-358.
[6]于晓坤,朴炫春,代月,等.贯叶连翘不定根反应器培养的初步研究[J].中国中药杂志,2012,37(4):3808-3811.
[7]杨世海,毕晓秀,杨慧洁,等.紫锥菊毛状根诱导及离体培养[J].中国药学杂志,2011,46(20):1557-1562.
[8]李慧娟,廉美兰,高日,等.盐浓度、碳源和磷酸盐浓度对东北刺人参不定根悬浮培养的影响[J].安徽农业科学,2012,40(22):11229-11230,11273.
[9]于丹.诱导子对东北刺人参不定根中有效物质含量的影响及抗氧化活性研究[D].延吉:延边大学,2015.
[10]李慧娟.东北刺人参不定根反应器培养及其抗氧化活性的研究[D].延吉:延边大学,2012.
[11]边黎明,施季森.植物生物反应器细胞悬浮培养研究进展[J].南京林业大学学报(自然科学版),2004,28(04):101-104.
[12]陈海伟.植物组织培养研究进展[J].赤峰学院学报(自然科学版),2007,23(06):16-17.
[13]岳才军,刘永春.通过培养毛状根生产药物的研究进展[J].黑龙江八一农垦大学学报,2003,15(01):20-24.
[14]杨静,孙皓.不同诱导子对黄芪悬浮培养细胞中黄芪多糖积累的影响[J].安徽农业科学,2014,42(26):8954-8956,8959.
[15]管桐,李贺,王洪秋,等.茉莉酸甲酯对生物反应器培养高山红景天愈伤组织有效物质积累的影响[J].延边大学农学学报,2017,39(01):24-28,34.
[16]邹凯,刘泽波,陈继光,等.甜叶菊毛状根的诱导培养及茉莉酸甲酯对其绿原酸类物质积累的影响[J].食品科学,2017,38(12):89-95.
[17]葛江丽,李成浩,刘芳,等.东北刺人参愈伤组织诱导和继代的研究[J].安徽农业科学,2012,40(30):14660,14667.
[18]苏力德,代那音台,乌恩齐,等.蒙药荠菜总黄酮的提取及其抗炎作用研究[J].内蒙古民族大学学报(自然科学版),2016,31(01):64-67.
[19]杨建华,孟新源,胡君萍,等.沙枣总酚抗炎作用研究[J].中国实验方剂学杂志,2013,19(18):263-266.