三七和银杏叶片对高原脱习服大鼠心功能及血清炎症因子的影响及其机制
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摘要
目的:研究三七、银杏叶片和红景天对高原脱习服大鼠心功能和白细胞介素-6(IL-6)、IL-10、肿瘤坏死因子-α(TNF-α)的影响,探讨高原脱习服的机制。方法:40只SD大鼠在模拟海拔5 000 m的低压氧舱内常规喂养3个月后随机分为三七组、银杏叶组、红景天组、未用药组(脱习服对照组),每组10只;另取10只SD大鼠常氧下喂养3个月作为未缺氧组。三七组、银杏叶组及红景天组分别按200 mg·kg-1剂量给予三七、银杏叶片和红景天混悬液灌胃,每日2次,连续10 d,未用药组则在出舱后常规喂养。10 d后通过心导管测定肺动脉压力(PAP)、左右室收缩压(VSP)和舒张压(VEDP)等。测压结束后,分离右心室(RV)、左心室加室间隔(LV+IVS),并依次称重,计算RV/(LV+IVS)比值即右心室肥厚指数(RVHI),并采血检测血清IL-6、IL-10和TNF-α、SOD和MDA。结果:与未缺氧组相比,未用药组、红景天组、三七组和银杏叶组RVHI、RVSP、RVEDP、m PAP及血清中IL-6、IL-10含量均有所增高(P<0.05或<0.01),未用药组、红景天组SOD均显著降低(P<0.01),MDA、TNF-α显著升高(P<0.01);与未用药组相比,三七组、银杏叶组、红景天组MDA、TNF-α降低(P <0.01),SOD升高(P <0.01);与银杏叶组、红景天组相比,三七组RV、RVHI、RVSP、RVEDP、LVSP、LVEDP、IL-10、TNF-α指标均有所降低或升高(P<0.05或<0.01)。结论:三七、银杏叶片和红景天可有效增强高原脱习服过程中心室功能、抑制高原脱习服大鼠炎症因子表达、提高机体抗氧化能力。
Objective: To study the effects of notoginseng,gingko leaf and rhodiola on cardiac functions and the serum inflammatory factors interleukin-6,interleukin-10,and TNF-α of rats with hypoxia deacclimatization,to explore the mechanism of hypoxia detoxification. Methods: Forty SD rats were randomly divided into notoginseng group( n = 10),gingko leaf group( n = 10),rhodiola group( n= 10) and high altitude control group( n = 10) after fed in a hypobaric hypoxia chamber( simulated altitude of 5 000 m) for 3 month,while 10 rats fed at normal pressure and oxygen environment for 3 month were used as the plain control group. Rats in notoginseng group,gingko leaf group and rhodiola group were treated with notoginseng,gingko leaf tablets or rhodiola suspension through intragastric administration( 200 mg/kg,twice a day,for 10 days). After the rats got intraperitoneal anesthesia with 10% urethane,5 min pulmonary artery pressure curve were traced continuously while pulmonary artery pressure( PAP). Left and right ventricular systolic pressure( VSP) and ventricular diastolic pressure( VEDP),the hemodynamic parameters were detected through a multi-channel physiological recorder. Serum tumor necrosis factor-α( TNF-α),interleukin-6( IL-6),interleukin-10( IL-10),superoxide dismutase( SOD) and malondialdehyde( MDA) were measured. Results: Right ventricular systolic pressure( RVSP),right ventricular end-diastolic pressure( RVEDP),mean pulmonary artery pressure( m PAP),left vent-ricular systolic pressure( LVSP),left ventricular end-diastolic pressure( LVEDP),IL-6,and IL-10 were higher in notoginseng group,gingko leafgroup,rhodiola group and high altitude control group than those in plain control group( P<0.05 or P<0.01). The contents of MDA and TNF-α were higher while the level of SOD was lower in rhodiola group and high altitude control group than those in plain control group( P<0.01). The contents of MDA and TNF-α were lower while the level of SOD was higher in notoginseng group,gingko leaf group and rhodiola group than those in high altitude control group( P<0.01). The levels of RV,RVHI,RVSP,RVEDP,LVSP,LVEDP,IL-10 and TNF-α were statistically changed in notoginseng group than those in gingko leaf group and rhodiola group( P < 0.05 or P < 0.01). Conclusion: Notoginseng,gingkoleaf and rhodiola can enhance antioxidant capacity of body and improve ventricular functions and Notoginseng,gingko leaf and rhodiola can effectively enhance the functions of ventricular and hypoxia tolerance and inhibit the expressions of inflammatory factors in rats during the hypoxia deacclimatization.
Objective: To study the effects of notoginseng,gingko leaf and rhodiola on cardiac functions and the serum inflammatory factors interleukin-6,interleukin-10,and TNF-α of rats with hypoxia deacclimatization,to explore the mechanism of hypoxia detoxification. Methods: Forty SD rats were randomly divided into notoginseng group( n = 10),gingko leaf group( n = 10),rhodiola group( n= 10) and high altitude control group( n = 10) after fed in a hypobaric hypoxia chamber( simulated altitude of 5 000 m) for 3 month,while 10 rats fed at normal pressure and oxygen environment for 3 month were used as the plain control group. Rats in notoginseng group,gingko leaf group and rhodiola group were treated with notoginseng,gingko leaf tablets or rhodiola suspension through intragastric administration( 200 mg/kg,twice a day,for 10 days). After the rats got intraperitoneal anesthesia with 10% urethane,5 min pulmonary artery pressure curve were traced continuously while pulmonary artery pressure( PAP). Left and right ventricular systolic pressure( VSP) and ventricular diastolic pressure( VEDP),the hemodynamic parameters were detected through a multi-channel physiological recorder. Serum tumor necrosis factor-α( TNF-α),interleukin-6( IL-6),interleukin-10( IL-10),superoxide dismutase( SOD) and malondialdehyde( MDA) were measured. Results: Right ventricular systolic pressure( RVSP),right ventricular end-diastolic pressure( RVEDP),mean pulmonary artery pressure( m PAP),left vent-ricular systolic pressure( LVSP),left ventricular end-diastolic pressure( LVEDP),IL-6,and IL-10 were higher in notoginseng group,gingko leafgroup,rhodiola group and high altitude control group than those in plain control group( P<0.05 or P<0.01). The contents of MDA and TNF-α were higher while the level of SOD was lower in rhodiola group and high altitude control group than those in plain control group( P<0.01). The contents of MDA and TNF-α were lower while the level of SOD was higher in notoginseng group,gingko leaf group and rhodiola group than those in high altitude control group( P<0.01). The levels of RV,RVHI,RVSP,RVEDP,LVSP,LVEDP,IL-10 and TNF-α were statistically changed in notoginseng group than those in gingko leaf group and rhodiola group( P < 0.05 or P < 0.01). Conclusion: Notoginseng,gingkoleaf and rhodiola can enhance antioxidant capacity of body and improve ventricular functions and Notoginseng,gingko leaf and rhodiola can effectively enhance the functions of ventricular and hypoxia tolerance and inhibit the expressions of inflammatory factors in rats during the hypoxia deacclimatization.
引文
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[15]Soon E,Holmes AM,Treacy CM,et al.Elevated levels of inflammatory cytokines predict survival in idiopathic and familial pulmonary arterial hypertension[J].Circulation,2010,122(9):920-927.
[16]Kobayashi K,Nishimura Y,Shimada T,et al.Effect of continuous positive airway pressure on soluble CD40 ligand in patients with obstructive sleep apnea syndrome[J].Chest,2006,129(3):632-637.
[17]Kamal Alam B,Bukhari AS,Assad S,et al.Functional outcome after decompressive craniectomy in patients with dominant or non-dominant malignant middle cerebral infarcts[J].Cureus,2017,9(1):e997.
[18]Mogi M,Iwai M,Horiuchi M.New paradigm for brain protection after stroke[J].Hypertension,2006,47(4):642-643.
[2]朱阿楠,王淑君,王园园,等.p38-MAPK信号通路在HHPH中的作用及三七总皂苷干预[J].中国应用生理学杂志,2012,28(1):79-83.
[3]Louajri A,Harraga S,Godot V,et al.The effect of ginkgo biloba extract on free radical production in hypoxic rats[J].Biol Pharm Bull,2001,24(6):710-712.
[4]Zhu L,Wei T,Chang X,et al.Effects of salidroside on myocardial injury in vivo in vitro via regulation of Nox/NF-kappaB/AP1 pathway[J].Inflammation,2015,38(4):1589-1598.
[5]Rakhit RD,Marber MS.Nitric oxide:an emerging role in cardioprotection[J]?Heart,2001,86(4):368-372.
[6]Li H,Deng CQ,Chen BY,et al.Total saponins of Panax notoginseng modulate the expression of caspases and attenuate apoptosis in rats following focal cerebral ischemiareperfusion[J].J Ethnopharmacol,2009,121(3):412-418.
[7]唐映红,张淑萍,梁燕,等.三七总皂苷对大鼠脑缺血再灌注后白细胞介素-1β和其相关因子及半胱氨酸天冬氨酸特异性蛋白酶表达的影响[J].中西医结合学报,2007,5(3):328-332.
[8]Si YC,Zhang JP,Xie CE,et al.Effects of Panax notoginseng saponins on proliferation and differentiation of rat hippocampal neural stem cells[J].Am J Chin Med,2011,39(5):999-1013.
[9]梁瑛琦,贾旭广,石璐,等.三七总皂苷对低氧大鼠肺动脉压和肺组织p38 MAPK表达的影响[J].中国病理生理杂志,2010,26(12):2438-2441.
[10]Isah T.Rethinking Ginkgo biloba L.:Medicinal uses and conservation[J].Pharmacogn Rev,2015,9(18):140-148.
[11]庞勇军,孙莉,谢文娟,等.红景天苷促进培养乳鼠心肌细胞肌质网钙离子的释放[J].中国病理生理杂志,2011,27(10):2021-2023.
[12]朱凌鹏,常厦云,何贺,等.红景天苷对脂多糖诱导的小鼠心肌损伤保护作用的研究[J].药学与临床研究,2015,23(1):13-15.
[13]Miller AF.Superoxide dismutases:active sites that save,but a protein that kills[J].Curr Opin Chem Biol,2004,8(2):162-168.
[14]梁瑛琦,黎关龙,王园园,等.三七总皂甙对低氧大鼠肺动脉压和肺组织ERK1/2表达的影响[J].中国应用生理学杂志,2012,28(2):101-102.
[15]Soon E,Holmes AM,Treacy CM,et al.Elevated levels of inflammatory cytokines predict survival in idiopathic and familial pulmonary arterial hypertension[J].Circulation,2010,122(9):920-927.
[16]Kobayashi K,Nishimura Y,Shimada T,et al.Effect of continuous positive airway pressure on soluble CD40 ligand in patients with obstructive sleep apnea syndrome[J].Chest,2006,129(3):632-637.
[17]Kamal Alam B,Bukhari AS,Assad S,et al.Functional outcome after decompressive craniectomy in patients with dominant or non-dominant malignant middle cerebral infarcts[J].Cureus,2017,9(1):e997.
[18]Mogi M,Iwai M,Horiuchi M.New paradigm for brain protection after stroke[J].Hypertension,2006,47(4):642-643.