MeJA对低温胁迫下冬小麦抗寒生理及关键基因表达量的影响
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摘要
植物激素作为一种信号分子,在植物响应低温胁迫中起重要作用。本研究以东农冬麦1号为材料,在田间三叶期用0.5、1.0和2.0 mmol·L~(-1) MeJA喷施小麦叶片,在大田自然降温条件下,探讨外源MeJA对其抗寒生理及 TaPDF1.2、 TaCOI1、 TaMYC2基因表达量的影响。结果表明,MeJA处理降低了低温胁迫下东农冬麦1号叶片及分蘖节的相对电导率和MDA含量,提高了脯氨酸、可溶性糖和可溶性蛋白的积累,提高了抗氧化酶SOD、POD和CAT活性;在不同处理浓度中,1.0 mmol·L~(-1) MeJA效果最好,处理后小麦返青率达91.5%;-10℃时,叶片和分蘖节内积累的保护性物质最多,保护性酶活性最强。东农冬麦1号分蘖节比叶片具有更强的抗寒性。此外,1.0 mmol·L~(-1)外源MeJA处理显著提高了东农冬麦1号叶片及分蘖节中 TaPDF1.2、 TaCOI1、 TaMYC2基因的表达量。
Phytohormone,as a signaling molecule,plays an important role in plants response to low temperature stress.Dongnong Dongmai 1 was used as experimental material in this study. To investigate the effect of exogenous MeJA on the cold tolerance physiology and the expression of TaPDF1.2, TaCOI1 and TaMYC2 genes in wheat,the leaves were sprayed with 0.5,1.0,and 2.0 mmol·L~(-1) MeJA at the trifoliate stage,and leaves and tillering node were collected at 5 ℃,0 ℃,-10 ℃,and-25 ℃ under natural cooling conditions in field. The results showed that MeJA treatment reduced the relative electrical conductivity and MDA content of leaves and tillering node of Dongnong Dongmai 1 under low temperature stress,increased the accumulation of proline,soluble sugar and soluble protein,and increased the activities of antioxidant enzymes SOD,POD and CAT.Among different treatment concentrations,1.0 mmol·L~(-1) MeJA performed best and the overwinter survival rate was 91.5%. At-10 ℃,the accumulation of protective substances in the leaves and tillering node was the highest,and the protective enzyme activity was the strongest. Compared to leaves, tillering node of Dongnong Dongmai 1 are more resistant to cold. Treatment with 1.0 mmol·L~(-1) exogenous MeJA significantly increased the expression of TaPDF1.2, TaCOI1,and TaMYC2 genes in leaves and tillering node of Dongnong Dongmai 1 under low temperature stress.
Phytohormone,as a signaling molecule,plays an important role in plants response to low temperature stress.Dongnong Dongmai 1 was used as experimental material in this study. To investigate the effect of exogenous MeJA on the cold tolerance physiology and the expression of TaPDF1.2, TaCOI1 and TaMYC2 genes in wheat,the leaves were sprayed with 0.5,1.0,and 2.0 mmol·L~(-1) MeJA at the trifoliate stage,and leaves and tillering node were collected at 5 ℃,0 ℃,-10 ℃,and-25 ℃ under natural cooling conditions in field. The results showed that MeJA treatment reduced the relative electrical conductivity and MDA content of leaves and tillering node of Dongnong Dongmai 1 under low temperature stress,increased the accumulation of proline,soluble sugar and soluble protein,and increased the activities of antioxidant enzymes SOD,POD and CAT.Among different treatment concentrations,1.0 mmol·L~(-1) MeJA performed best and the overwinter survival rate was 91.5%. At-10 ℃,the accumulation of protective substances in the leaves and tillering node was the highest,and the protective enzyme activity was the strongest. Compared to leaves, tillering node of Dongnong Dongmai 1 are more resistant to cold. Treatment with 1.0 mmol·L~(-1) exogenous MeJA significantly increased the expression of TaPDF1.2, TaCOI1,and TaMYC2 genes in leaves and tillering node of Dongnong Dongmai 1 under low temperature stress.
引文
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[18]吴晨雨,何俊娜,钟雄辉.唐菖蒲茉莉素受体基因 GhCOI1的克隆与表达分析[J].园艺学报,2015,42(2):289.WU C Y,HE J N,ZHONG X H.Cloning and expression analysis of the jasmoid receptor gene GhCOI1 in gladiolus hybridus [J].Journal of Horticulture,2015,42(2):289.
[19]WANG Z,CAO G,WANG X,et al.Identification and characterization of COI1-dependent transcription factor genes involved in JA-mediated response to wounding in Arabidopsis plants [J].Plant Cell Reports,2008,27(1):125.
[20]HU T,WANG W,CAO K,et al.OsCOI1,a putative COI1 in rice,show MeJA and ABA dependent expression [J].Progress in Biochemistry & Biophysics,2006,33(4):388.
[2]齐付国,李建民,段留生,等.冠菌素和茉莉酸甲酯诱导小麦幼苗低温抗性的研究[J].西北植物学报,2006,26(9):1776.QI F G,LI J M,DUAN L S,et al.Study on cold resistance of wheat seedlings induced by coronatine and methyl jasmonate [J].Acta Botanica Boreali-Occidentalia Sinica,2006,26(9):1776.
[3]ZHAO Y,DONG W,ZHANG N,et al.A wheat allene oxide cyclase gene enhances salinity tolerance via jasmonate signaling [J].Plant Physiology,2014,164(2):1068.
[4]DU H,LIU H,XIONG L.Endogenous auxin and jasmonic acid levels are differentially modulated by abiotic stresses in rice [J].Frontiers in Plant Science,2013,4(9):397.
[5]HU Y,JIANG L,WANG F,et al.Jasmonate regulates the inducer of cbf expression-C-repeat binding factor/DRE binding factor1 cascade and freezing tolerance in Arabidopsis [J].Plant Cell,2013,25(8):2907.
[6] GFELLER A,LIECHTI R,FARMER E E.Arabidopsis jasmonate signaling pathway [J].Sciences Stke Signal Transduction Knowledge Environment,2006,3(109):cm4.
[7] SONG S,HUANG H,GAO H,et al.Interaction between MYC2 and ETHYLENE INSENSITIVE3 modulates antagonism between jasmonate and ethylene signaling in Arabidopsis [J].Plant Cell,2014,26(1):263.
[8]KEMAL K,JOHN M M.MYC2:The master in action [J].Molecular Plant,2013,6(3):686.
[9]卢宝伟.东农冬麦1号越冬期间的microRNA高通量测序及生物信息学分析[D].哈尔滨:东北农业大学,2012:46.LU B W.MicroRNA high-throughput sequencing and bioinformatics analysis of Dongnong Dongmai 1 during winter [D].Harbin:Northeast Agricultural University,2012:46.
[10]黄儒,苍晶,于晶,等.冬小麦小RNA高通量测序及生物信息学分析[J].植物学报,2014,49(1):8.HUANG R,CANG J,YU J,et al.Small RNA high-throughput sequencing and bioinformatics analysis of winter wheat[J].Chinese Journal of Botany,2014,49(1):8.
[11]卢秋巍,徐庆华,钟昊,等.冬小麦抗寒相关microRNA生物信息学预测分析与表达特征的验证[J].植物生理学报,2015(5):747.LU Q W,XU Q H,ZHONG H,et al.Prediction and analysis of cold-resistance-related microRNA bioinformatics in winter wheat and validation of its expression characteristics [J].Chinese Journal of Plant Physiology,2015(5):747.
[12]张以顺,黄霞,陈云凤.植物生理学实验教程[M].北京:高等教育出版社,2009:21.ZHANG Y S,HUANG X,CHEN Y F.Experimental course of plant physiology [M].Beijing:Higher Education Press,2009:21.
[13]吴冰,苍晶,张达,等.SA处理对低温胁迫下冬小麦(Triticum aestivum L.)蔗糖代谢的影响[J].植物生理学报,2015,51(4):537.WU B,CANG J,ZHANG D,et al.Effects of SA treatment on the sucrose metabolism of winter wheat(Triticum aestivum l.) under low temperature stress [J].Journal of Plant Physiology,2015,51(4):537.
[14]ZENG Y,YU J,CANG J,et al.Detection of sugar accumulation and expression levels of correlative key enzymes in winter wheat at low temperature [J].Biosci Biotechnol Biochem,2011,75(4):681.
[15]DAR T A,UDDIN M,KHAN M M A,et al.Jasmonates counter plant stress:A review [J].Environmental & Experimental Botany,2015,115:49.
[16] MIN D D,LI F J,ZHANG X H,et al. SlMYC2 involved in MeJA-induced tomato fruit chilling tolerance [J].Journal of Agricultural & Food Chemistry,2018,66(12):3110.
[17]WASTERNACK C.Action of jasmonates in plant stress responses and development-applied aspects [J].Biotechnology Advances,2014,32(1):1.
[18]吴晨雨,何俊娜,钟雄辉.唐菖蒲茉莉素受体基因 GhCOI1的克隆与表达分析[J].园艺学报,2015,42(2):289.WU C Y,HE J N,ZHONG X H.Cloning and expression analysis of the jasmoid receptor gene GhCOI1 in gladiolus hybridus [J].Journal of Horticulture,2015,42(2):289.
[19]WANG Z,CAO G,WANG X,et al.Identification and characterization of COI1-dependent transcription factor genes involved in JA-mediated response to wounding in Arabidopsis plants [J].Plant Cell Reports,2008,27(1):125.
[20]HU T,WANG W,CAO K,et al.OsCOI1,a putative COI1 in rice,show MeJA and ABA dependent expression [J].Progress in Biochemistry & Biophysics,2006,33(4):388.