一种小型化低成本免疫荧光分析仪的设计
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摘要
目的研制一款小型化、低成本、带自动抛样功能的免疫荧光分析仪。方法该免疫荧光分析仪采用非共聚焦方式的光路设计。光源位于光模块两侧,光电二极管位于试纸条正上方。光模块使用微动开关作为插入检测,使用推杆电机控制测试卡的移动,配合硅光二极管实现对测试卡的扫描。结果实践表明,该仪器精密度上CV值不大于5%,低值与中值的准确性方面偏差不大于5%,在线性范围内梯度液相关系数大于0.99,光路零部件固定良好,运动机构简单,带有插卡自动检测、废卡抛样等功能。结论仪器采用的新设计是有效的,在食品安全、动物疫病等免疫荧光实验领域可广泛应用。
Objective To design a miniaturized, low-cost immunofluorescence analyzer with automatic discarding function.Methods The immunofluorescence analyzer was designed with a non-confocal optical path. The light source was located on both sides of the optical module, and the photodiode was located directly above the test strip. The optical module used micro switch to detect the insertion of test trip, used push-rod motor to control the movement of the test strip, and realized the scanning process of the test card with the silicon photodiode. Results The results showed that the precision of the analyzer had a CV value of less than 5%, the accuracy of the low-value and intermediate-value was not more than 5%, and the gradient ?uid correlation coefficient was more than 0.99 in the linear range. The immunofluorescence analyzer had well fixed optical module,simple movement mechanism, with the functions of automatic detection of card insert and automatic discarding of waste card.Conclusion The new design of the instrument is effective, and can be widely applied in the immuno?uorescence of food safety and animal diseases.
Objective To design a miniaturized, low-cost immunofluorescence analyzer with automatic discarding function.Methods The immunofluorescence analyzer was designed with a non-confocal optical path. The light source was located on both sides of the optical module, and the photodiode was located directly above the test strip. The optical module used micro switch to detect the insertion of test trip, used push-rod motor to control the movement of the test strip, and realized the scanning process of the test card with the silicon photodiode. Results The results showed that the precision of the analyzer had a CV value of less than 5%, the accuracy of the low-value and intermediate-value was not more than 5%, and the gradient ?uid correlation coefficient was more than 0.99 in the linear range. The immunofluorescence analyzer had well fixed optical module,simple movement mechanism, with the functions of automatic detection of card insert and automatic discarding of waste card.Conclusion The new design of the instrument is effective, and can be widely applied in the immuno?uorescence of food safety and animal diseases.
引文
[1]潘星,郑忠亮,吴琼水.免疫荧光定量分析仪设计[J].电子技术应用,2014,11(40):19-22.
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[10]刘威龙,孙明磊,王頔,等.基于STM32的分布式步进电机控制系统设计[J].数字技术与应用,2012,(3):12-14.
[11]吴刚,姜瞻梅,霍贵成,等.胶体金免疫层析技术在食品检测中的应用[J].食品工业科技,2007,(12):216-218.
[12]梁晓威,程威,孙云,等.全自动免疫荧光分析仪检测苯丙氨酸的性能验证[J].南京医科大学学报(自然科学版),2018,38(5):134-137.
[13]段秀群,田欢,龚国富.两种仪器测定血清C反应蛋白的性能比对分析[J].中国医疗设备,2017,32(2):87-90.
[14]杨泽权.降钙素原乳胶增强免疫比浊法与免疫荧光法的相关性[J].国际检验医学杂志,2016,(3):416-417.
[15]蒋玲丽,唐大海,张健,等.5种C反应蛋白快速检测系统检测性能比较[J].检验医学,2016,31(4):293-298.
[16]汪怀周,陈燕,岳展伊,等.C反应蛋白测定两种检测系统间结果的比较[J].现代检验医学杂志,2016,31(3):127-130.
[17]卢锦沛.不同检测系统C反应蛋白测定结果可比性研究[J]国际检验医学杂志,2016,37(8):1110-1111.
[18]胡静,李立新,罗俐梅,等.全自动间接免疫荧光法分析仪检测抗核抗体与人工判读的对比研究[J].华西医学,2016,31(6):1084-1087.
[2]黄晓群.酶联免疫法、金标法、时间分辨免疫荧光分析法测定HBs Ag对比分析[J].中国保健营养:临床医学学刊,2009,18(5):7-9.
[3]汪宏良,陈丽峰,胡芳.生物化学分析仪检测结果的临床评价[J].检验医学与临床,2007,4(6):558-559.
[4]龙宪和.对IMMULITEⅠ型化学发光酶免疫技术中检测原理及相关内容的初评[J].标记免疫分析与临床,2005,12(1):50-52
[5]张超,刘艳珍.免疫荧光层析定量检测系统的改进研究[J].中国医药科学,2014,(23):157-161.
[6]钱英超,陈丽华.免疫荧光干式定量法检测全血CRP的临床应用评估[J].中国实用医药,2014,(32):76-77.
[7]刘云峰,邓清华,杜利容,等.时间分辨免疫荧光分析法影响因素初探[J].泸州医学院学报,2007,30(2):124-126.
[8]杭建峰,吴英松,徐伟文,等.总PSA时间分辨免疫荧光分析法的建立[J].现代免疫学,2005,25(5):402-405.
[9]黄华伟,杜美菊.免疫荧光分析的研究进展[J].应用化工2007,36(4):394-397.
[10]刘威龙,孙明磊,王頔,等.基于STM32的分布式步进电机控制系统设计[J].数字技术与应用,2012,(3):12-14.
[11]吴刚,姜瞻梅,霍贵成,等.胶体金免疫层析技术在食品检测中的应用[J].食品工业科技,2007,(12):216-218.
[12]梁晓威,程威,孙云,等.全自动免疫荧光分析仪检测苯丙氨酸的性能验证[J].南京医科大学学报(自然科学版),2018,38(5):134-137.
[13]段秀群,田欢,龚国富.两种仪器测定血清C反应蛋白的性能比对分析[J].中国医疗设备,2017,32(2):87-90.
[14]杨泽权.降钙素原乳胶增强免疫比浊法与免疫荧光法的相关性[J].国际检验医学杂志,2016,(3):416-417.
[15]蒋玲丽,唐大海,张健,等.5种C反应蛋白快速检测系统检测性能比较[J].检验医学,2016,31(4):293-298.
[16]汪怀周,陈燕,岳展伊,等.C反应蛋白测定两种检测系统间结果的比较[J].现代检验医学杂志,2016,31(3):127-130.
[17]卢锦沛.不同检测系统C反应蛋白测定结果可比性研究[J]国际检验医学杂志,2016,37(8):1110-1111.
[18]胡静,李立新,罗俐梅,等.全自动间接免疫荧光法分析仪检测抗核抗体与人工判读的对比研究[J].华西医学,2016,31(6):1084-1087.