黄瓜蜡质合成基因CsCER10的表达模式及生物学信息分析
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摘要
前人应用荧光定量技术在黄瓜中筛选出了可能与蜡质合成相关的基因CsCER10。在前人研究的基础上,利用荧光定量技术分析CsCER10在黄瓜不同组织及盐胁迫、干旱、低温等非生物胁迫下的表达模式,利用DNAMAN,MEGA5及SPDBV等软件对CsCER10的生物学信息进行分析,为黄瓜表皮蜡质合成的分子机制提供理论基础。结果表明,CsCER10在黄瓜的各个器官均有表达,在叶片和果皮中的表达量较高;CsCER10受非生物胁迫的诱导,在遭受干旱、低温及盐胁迫时表达量均呈上升趋势。研究结果与前人对蜡质合成基因的研究结果一致,可推断CsCER10是与蜡质合成相关的基因。
Previous studies have screened CsCER10 gene related to wax synthesis by quantitative RT-PCR in cucumber. On the basis of previous studies, the paper analyzed the expression patterns of CsCER10 in different tissues and abiotic stresses such as salt stress, drought and low temperature of cucumber by quantitative RT-PCR technology. The biological information of CsCER10 was analyzed by DNAMAN, MEGA5 and SPDBV software, which provided a theoretical basis for the molecular mechanism of wax synthesis in cucumber epidermis waxy synthesis. The results showed that CsCER10 was expressed in all organs of cucumber, with the highest expression in leaves and pericarps. CsCER10 was induced by abiotic stress and showed an upward trend under drought, low temperature and salt stress. The results in this study are consistent with previous studies on wax synthesis genes, and it can be inferred that CsCER10 is a wax synthesis related gene.
Previous studies have screened CsCER10 gene related to wax synthesis by quantitative RT-PCR in cucumber. On the basis of previous studies, the paper analyzed the expression patterns of CsCER10 in different tissues and abiotic stresses such as salt stress, drought and low temperature of cucumber by quantitative RT-PCR technology. The biological information of CsCER10 was analyzed by DNAMAN, MEGA5 and SPDBV software, which provided a theoretical basis for the molecular mechanism of wax synthesis in cucumber epidermis waxy synthesis. The results showed that CsCER10 was expressed in all organs of cucumber, with the highest expression in leaves and pericarps. CsCER10 was induced by abiotic stress and showed an upward trend under drought, low temperature and salt stress. The results in this study are consistent with previous studies on wax synthesis genes, and it can be inferred that CsCER10 is a wax synthesis related gene.
引文
[1]陈伟,刘德春,杨莉,等.植物表皮蜡质及相关基因研究进展[J].植物生理学报,2016,52(8):1117-1127.
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[11]胡晓敏,张志飞,饶力群,等.植物角质层蜡质合成与调控的分子生物学研究进展[J].植物科学学报,2007,25(4):377-380.
[12]LACEY S,LJERKA K,REINHARD J. Sealing plant surfaces:Cuticular wax formation by epidermal cell[J]. Plant Mol Biol,2008,59(1):683-707.
[13] ZHENG H,ROWLAND O,KUNST L. Disruptions of the Arabidopsis enoyl-CoA reductase gene reveal an essential role for very-long-chain fatty acidsynthesis in cell expansion during plant morphogenesis[J]. Plant Cell,2005,17(5):1467-1481.
[14]倪郁,宋超,李加纳,等. UV-B辐射增强对拟南芥表皮蜡质的影响[J].生态学报,2015,35(5):1505-1512.
[15] WANG W J,ZHANG Y,XU C,et al. Cucumber ECERIFERUM1(CsCER1),which influences the cuticle properties and drought tolerance of cucumber,plays a key role in VLC alkanes biosynthesis[J]. Plant Mol Biol,2015,87(3):219-233.
[16] WANG W,LIU X W,GAI X S,et al. Cucumis sativus L. WAX2plays a pivotal role in wax biosynthesis,infuencing pollen fertility and plant biotic and abiotic stress responses[J]. Plant Cell Physiol,2015,56(7):1339-1354.
[17]樊蕾,高志英.黄瓜不定芽诱导试验[J].山西农业科学,2013,41(5):438-439.
[18]华智锐,李小玲.外源IAA对盐胁迫黄芩幼苗生长的生理效应[J].山西农业科学,2019,47(3):323-328,404.
[19]牟雪姣,张强,吴燕,等.外源CO对干旱胁迫下黄瓜种子萌发生长的缓解效应[J].华北农学报,2018,33(5):172-177.
[20]朱琳,袁梦,高红秀,等.水稻苗期低温应答转录组分析[J].华北农学报,2018,33(5):44-55.
[21]姜上川,梅超,王小芳,等.拟南芥SOAR1基因响应ABA与渗透胁迫的表达分析[J].河南农业科学,2016,45(2):29-34.
[22]朱素琴,马红,田洋,等.玉米ZmGLDH基因的克隆与表达分析[J].河南农业科学,2017,46(12):17-23.
[23]周玲艳,姜大刚,李静,等.逆境处理下水稻叶角质层蜡质积累及其与蜡质合成相关基因OsGL1表达的关系[J].作物学报,2012,38(6):1115-1120.
[24]刘小凤,安静波,张立新,等.黄瓜调控蜡质合成相关基因CsCER7的克隆与表达分析[J].园艺学报,2014(4):661-671.
[25]KUNST L,SAMUELS A L. Biosynthesis and secretion of plant cuticular wax[J]. Progress in Lipid Research,2003,42(1):51-80.
[2]李魏强,张正斌,李景娟.植物表皮蜡质与抗旱及其分子生物学[J].植物生理与分子生物学学报,2006,32(5):505-512.
[3]胡晓君,张正斌,刘文,等.植物表皮蜡质及极长链脂肪酸类物质的研究进展[J].安徽农业科学,2013,41(12):5176-5178.
[4]TEUSINK R S,RAHMAN M,BRESSAN R A,et al. Cuticular waxes on Arabidopsis thaliana close relatives Thellungiella halophila and Thellungiella parvula[J]. International Journal of Plant Sciences,2002,163(2):309-315.
[5] VAN M C,HAN H,JETTER R. Development of the cuticular wax during growth of Kalanchoe daigremontiana(Hamet rt Perr. De la Bathie)leaves[J]. Plant Cell and Environment,2009,32(1):73-81.
[6] WEN M,BUSCHHAUS C,JETTER R. Naontubules on plant surfaces:Chemical composition of epicuticular wax crystals on needles of Taxus baccata L[J]. Phytochemistry,2006,67(16):1808-1817.
[7]王永平,张爱民,廖芳芳,等.植物表皮蜡质合成和运输途径研究进展[J].中国农学通报,2012,28(3):225-232.
[8]杨贤鹏,王宙雅,高翔,等.植物表皮蜡质生物合成及调控[J].中国生物工程杂志,2016,36(9):75-80.
[9]曾琼,刘德春,刘勇.植物角质层蜡质的化学组成研究综述[J].生态学报,2013,33(17):5133-5140.
[10]岑斌,王慧中.植物蜡质合成与分泌的研究进展[J].科技通报,2009,25(3):265-270.
[11]胡晓敏,张志飞,饶力群,等.植物角质层蜡质合成与调控的分子生物学研究进展[J].植物科学学报,2007,25(4):377-380.
[12]LACEY S,LJERKA K,REINHARD J. Sealing plant surfaces:Cuticular wax formation by epidermal cell[J]. Plant Mol Biol,2008,59(1):683-707.
[13] ZHENG H,ROWLAND O,KUNST L. Disruptions of the Arabidopsis enoyl-CoA reductase gene reveal an essential role for very-long-chain fatty acidsynthesis in cell expansion during plant morphogenesis[J]. Plant Cell,2005,17(5):1467-1481.
[14]倪郁,宋超,李加纳,等. UV-B辐射增强对拟南芥表皮蜡质的影响[J].生态学报,2015,35(5):1505-1512.
[15] WANG W J,ZHANG Y,XU C,et al. Cucumber ECERIFERUM1(CsCER1),which influences the cuticle properties and drought tolerance of cucumber,plays a key role in VLC alkanes biosynthesis[J]. Plant Mol Biol,2015,87(3):219-233.
[16] WANG W,LIU X W,GAI X S,et al. Cucumis sativus L. WAX2plays a pivotal role in wax biosynthesis,infuencing pollen fertility and plant biotic and abiotic stress responses[J]. Plant Cell Physiol,2015,56(7):1339-1354.
[17]樊蕾,高志英.黄瓜不定芽诱导试验[J].山西农业科学,2013,41(5):438-439.
[18]华智锐,李小玲.外源IAA对盐胁迫黄芩幼苗生长的生理效应[J].山西农业科学,2019,47(3):323-328,404.
[19]牟雪姣,张强,吴燕,等.外源CO对干旱胁迫下黄瓜种子萌发生长的缓解效应[J].华北农学报,2018,33(5):172-177.
[20]朱琳,袁梦,高红秀,等.水稻苗期低温应答转录组分析[J].华北农学报,2018,33(5):44-55.
[21]姜上川,梅超,王小芳,等.拟南芥SOAR1基因响应ABA与渗透胁迫的表达分析[J].河南农业科学,2016,45(2):29-34.
[22]朱素琴,马红,田洋,等.玉米ZmGLDH基因的克隆与表达分析[J].河南农业科学,2017,46(12):17-23.
[23]周玲艳,姜大刚,李静,等.逆境处理下水稻叶角质层蜡质积累及其与蜡质合成相关基因OsGL1表达的关系[J].作物学报,2012,38(6):1115-1120.
[24]刘小凤,安静波,张立新,等.黄瓜调控蜡质合成相关基因CsCER7的克隆与表达分析[J].园艺学报,2014(4):661-671.
[25]KUNST L,SAMUELS A L. Biosynthesis and secretion of plant cuticular wax[J]. Progress in Lipid Research,2003,42(1):51-80.