小反刍兽疫疫苗对不同日龄湖羊的免疫效果研究
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摘要
为研究小反刍兽疫(PPR)疫苗的临床免疫效果,本研究从PPR活疫苗免疫的24个湖羊场采集了495份湖羊血清样品进行抗体检测,另外选择不同日龄的羔羊进行PPR免疫实验,分别于免疫后的7 d、14 d、21 d、35 d、210 d进行抗体跟踪监测。结果显示,90日龄以后免疫PPR的湖羊抗体合格率和抗体水平明显高于90日龄之前免疫的湖羊,在免疫7 d后,免疫抗体合格率即能够达到91.30%,免疫后14 d抗体水平达到峰值,免疫合格率达100%,随后抗体水平趋于稳定,并呈缓慢下降趋势,7个月后免疫合格率降至88.24%。结果表明,具有母源抗体的羔羊的最佳免疫时间为100日龄左右,群体保护率高,免疫期长。本研究为湖羊制定合理的PPR免疫程序提供了实验依据。
In order to study the immunization effects of the live peste des petits ruminants(PPR) vaccine, a total of 495 serum samples of Hu sheep were collected from 24 farms in Zhejiang after vaccination to detect specific antibodies against PPR. Besides,lambs at different day-old were vaccinated with PPR live vaccine, and the antibody titers were detected at 7, 14, 21, 35 and 210 days post vaccination. The results showed that the lambs vaccinated after 90 day-old had higher positive antibody rates than that of the lambs vaccinated before 90 day-old. The rates of desired threshold antibody level(DTAL) in lambs vaccinated after 90 day-old reached to 91.30% even at 7 days post vaccination, and to 100% at 14 days post vaccination, in a peak levels. Moreover, the rate of DTAL still kept in 88.24% after 7 months later post vaccination. Therefore, the reasonable vaccination schedule for lambs with maternal antibody against PPR is around 100-day-old.
In order to study the immunization effects of the live peste des petits ruminants(PPR) vaccine, a total of 495 serum samples of Hu sheep were collected from 24 farms in Zhejiang after vaccination to detect specific antibodies against PPR. Besides,lambs at different day-old were vaccinated with PPR live vaccine, and the antibody titers were detected at 7, 14, 21, 35 and 210 days post vaccination. The results showed that the lambs vaccinated after 90 day-old had higher positive antibody rates than that of the lambs vaccinated before 90 day-old. The rates of desired threshold antibody level(DTAL) in lambs vaccinated after 90 day-old reached to 91.30% even at 7 days post vaccination, and to 100% at 14 days post vaccination, in a peak levels. Moreover, the rate of DTAL still kept in 88.24% after 7 months later post vaccination. Therefore, the reasonable vaccination schedule for lambs with maternal antibody against PPR is around 100-day-old.
引文
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[2]宋建德,袁丽萍,刘陆世,等.全球小反刍兽疫流行状况和防控策略[J].中国动物检疫,2015,32(7):8-12.
[3]信爱国,杨仁标,向文彬.小反刍兽疫研究进展[J].中国预防兽医学报,2003,25(2):152-154.
[4]王志亮,包静月,吴晓东,等.我国首例小反刍兽疫诊断报告[J].中国动物检疫,2007,24(8):24-26.
[5]Ata F A,Sumry S,King G J,et al.Duration of maternal immunity to peste des petits ruminants[J].Vet Rec,1989,124:590-591.
[6]Bodjo S C,Couacyhymann E,Koffi M Y,et al.Assessment of the duration of maternal antibodies specific to the homologous peste des petits ruminant vaccine"Nigeria 75/1" in Djallonkélambs[J].Biokemistri,2006,18(2):99-103.
[7]Balamurugan V,Sen A,Venkatesan G,et al.Study on passive immunity:Time of vaccination in kids born to goats vaccinated against Peste des petits ruminants[J].Virol Sin,2012,27(4):228-233.