小反刍兽疫活疫苗二次免疫效果评价
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摘要
为研究已免疫小反刍兽疫(PPR)活疫苗的羊群经二次免疫后PPR抗体水平的变化趋势,对初免21 d羊群进行了第二次免疫,同时监测初免21 d及二免后3 d、7 d、14 d、21 d的血清中和抗体。结果显示,一次免疫羊21 d PPR中和抗体滴度平均可达5.18±2.87,阳性率为84%;二次免疫后3 d、7 d、14 d、21 d,PPR抗体呈上升趋势,平均滴度分别为5.65±2.56、6.95±1.82、7.28±1.18、8.12±1.31,阳性率分别为90%、100%、100%、100%。一免试验组21 d~323 d PPR抗体阳性率为83%,二免试验组在二次免疫7 d PPR抗体阳性率达到100%,并且维持到21 d。试验表明,活疫苗二次免疫增强了免疫效果,对已经有PPR抗体的羊进行二次免疫,可明显提升免疫抗体阳性率。
In order to research the antibody change tendency of secondary immunization,the sheep were injected with live attenuated peste des petits ruminants( PPR) vaccine at the 21 st day after primary immunization. The PPR antibody at the 21 st day after primary immunization and at the 3 rd,7 th,14 th,21 st day after secondary immunization were detected respectively. The results indicated that the PPR antibody at 21 st day after primary immunization reached to 5.18±2.87 and the positive rate was 84%. The PPR antibody level from the 3 rd day to the 21 st day after secondary immunization increased and the average titer were 5. 65 ± 2. 56,6. 95 ± 1. 82,7. 28 ±1.18,8.12 ± 1. 31 respectively,positive rate was 90%,100%,100%,100% respectively. The PPR antibody positive rate at primary immunization group from 21 days to 323 days was 83%,PPR antibody positive rate reached to 100% at the 7 th day after secondary immunization and kept high level to the 21 st day,which showed that secondary immunization could enhance the effect of vaccine,and increase the positive rate of immune antibodies.
In order to research the antibody change tendency of secondary immunization,the sheep were injected with live attenuated peste des petits ruminants( PPR) vaccine at the 21 st day after primary immunization. The PPR antibody at the 21 st day after primary immunization and at the 3 rd,7 th,14 th,21 st day after secondary immunization were detected respectively. The results indicated that the PPR antibody at 21 st day after primary immunization reached to 5.18±2.87 and the positive rate was 84%. The PPR antibody level from the 3 rd day to the 21 st day after secondary immunization increased and the average titer were 5. 65 ± 2. 56,6. 95 ± 1. 82,7. 28 ±1.18,8.12 ± 1. 31 respectively,positive rate was 90%,100%,100%,100% respectively. The PPR antibody positive rate at primary immunization group from 21 days to 323 days was 83%,PPR antibody positive rate reached to 100% at the 7 th day after secondary immunization and kept high level to the 21 st day,which showed that secondary immunization could enhance the effect of vaccine,and increase the positive rate of immune antibodies.
引文
[1]Naveen K,Sunil M,Sudhir K K,et al.Peste des petits ruminants virus infection of small ruminants:a comprehensive review[J].Viruses,2014,6(6):2287-2327.
[2]Begum S S,Mahato G,Sharma P.Assessment of immune response to a lyophilized peste-des-petits-ruminants virus vaccine in three different breeds of goats[J].Vet World,2016,9(6):568-571.
[3]Balamurugan V,Hemadri D,Gajendragad M R,et al.Diagnosis and control of peste des petits ruminants:a comprehensive review[J].Virus Disease,2014,25(1):39-56.
[4]Satya P,Murali M,Eda A,et al.Emergence of PPR and its threat to Europe[J].Small Rumin Res,2016,142:16-21.
[5]Parida S,Muniraju M,Mahapatra M,et al.Peste des petits ruminants[J].Vet Microbiol,2015,181(1/2):90-106.
[6]吴锦艳,尚佑军,田宏,等.2007-2014年国内外小反刍兽疫流行现状及分析[J].中国兽医学报,2016,(4):687-693.Wu J Y,Shang Y J,Tian H,et al.The epidemic situation and analysis of peste des petits ruminants in China and worldwide from2007 to 2014[J].Chinese Journal of Veterinary Science,2016,(4):687-693.
[7]刘永宏,赵丽,曹胜波,等.小反刍兽疫在中国的流行趋势及应对措施[J].动物医学进展,2015,36(1):110-113.Liu Y H,Zhao L,Cao S B,et al.Prevelant trends and countermeasures of peste des petits ruminants in China[J].Progress In Veterinary Medicine,2015,36(1):110-113.
[8]汪萍,尹传振,柏庆,等.阻断ELISA与病毒中和试验检测PPR抗体的比较分析[J].中国预防兽医学报,2016,38(8):658-660.Wang P,Yin C Z,Bai Q,et al.Comparison of ELISA with the virus neutralization test for detecting serum antibody to peste des petits ruminants[J].Chinese Journal of Preventive Veterinary Medicine,2016,38(8):658-660.
[9]宋跃君,徐玉,张远洲,等.小反刍兽疫疫苗临床免疫效果试验[J].动物医学进展,2016,37(6):77-79.Song Y J,Xu Y,Zhang Y Z,et al.Clinical immune effect of peste des petits ruminants vaccine[J].Progress In Veterinary Medicine,2016,37(6):77-79.
[10]何利昆,段亚良,谷志大,等.辽宁省小反刍兽疫疫苗临床免疫效果跟踪试验及抗体水平动态观察[J].现代畜牧兽医,2014,(8):14-18.He L K,Duan Y L,Gu Z D,et al.Immune efficacy and antibody level of Peste des petits ruminants vaccine in a immunological experiment in Liaoning province[J].Modern Journal of Animal Husbandry and Veterinary Medicine,2014,(8):14-18.
[11]徐娅萍,邱寒峰,俞乾挺,等.小反刍兽疫疫苗对不同日龄湖羊的免疫效果研究[J].中国预防兽医学报,2016,38(8):646-648.Xu,Y P,Qiu H F,Yu H T,et al.The immunization effects on the antibody induced by vaccine of peste des petits ruminants in Hu sheep at different day-old[J].Chinese Journal of Preventive Veterinary Medicine,2016,38(8):646-648.
[2]Begum S S,Mahato G,Sharma P.Assessment of immune response to a lyophilized peste-des-petits-ruminants virus vaccine in three different breeds of goats[J].Vet World,2016,9(6):568-571.
[3]Balamurugan V,Hemadri D,Gajendragad M R,et al.Diagnosis and control of peste des petits ruminants:a comprehensive review[J].Virus Disease,2014,25(1):39-56.
[4]Satya P,Murali M,Eda A,et al.Emergence of PPR and its threat to Europe[J].Small Rumin Res,2016,142:16-21.
[5]Parida S,Muniraju M,Mahapatra M,et al.Peste des petits ruminants[J].Vet Microbiol,2015,181(1/2):90-106.
[6]吴锦艳,尚佑军,田宏,等.2007-2014年国内外小反刍兽疫流行现状及分析[J].中国兽医学报,2016,(4):687-693.Wu J Y,Shang Y J,Tian H,et al.The epidemic situation and analysis of peste des petits ruminants in China and worldwide from2007 to 2014[J].Chinese Journal of Veterinary Science,2016,(4):687-693.
[7]刘永宏,赵丽,曹胜波,等.小反刍兽疫在中国的流行趋势及应对措施[J].动物医学进展,2015,36(1):110-113.Liu Y H,Zhao L,Cao S B,et al.Prevelant trends and countermeasures of peste des petits ruminants in China[J].Progress In Veterinary Medicine,2015,36(1):110-113.
[8]汪萍,尹传振,柏庆,等.阻断ELISA与病毒中和试验检测PPR抗体的比较分析[J].中国预防兽医学报,2016,38(8):658-660.Wang P,Yin C Z,Bai Q,et al.Comparison of ELISA with the virus neutralization test for detecting serum antibody to peste des petits ruminants[J].Chinese Journal of Preventive Veterinary Medicine,2016,38(8):658-660.
[9]宋跃君,徐玉,张远洲,等.小反刍兽疫疫苗临床免疫效果试验[J].动物医学进展,2016,37(6):77-79.Song Y J,Xu Y,Zhang Y Z,et al.Clinical immune effect of peste des petits ruminants vaccine[J].Progress In Veterinary Medicine,2016,37(6):77-79.
[10]何利昆,段亚良,谷志大,等.辽宁省小反刍兽疫疫苗临床免疫效果跟踪试验及抗体水平动态观察[J].现代畜牧兽医,2014,(8):14-18.He L K,Duan Y L,Gu Z D,et al.Immune efficacy and antibody level of Peste des petits ruminants vaccine in a immunological experiment in Liaoning province[J].Modern Journal of Animal Husbandry and Veterinary Medicine,2014,(8):14-18.
[11]徐娅萍,邱寒峰,俞乾挺,等.小反刍兽疫疫苗对不同日龄湖羊的免疫效果研究[J].中国预防兽医学报,2016,38(8):646-648.Xu,Y P,Qiu H F,Yu H T,et al.The immunization effects on the antibody induced by vaccine of peste des petits ruminants in Hu sheep at different day-old[J].Chinese Journal of Preventive Veterinary Medicine,2016,38(8):646-648.