液体发酵桑黄总黄酮对抗肿瘤活性及细胞周期的影响
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摘要
研究液体发酵桑黄菌落所提取的黄酮抗肿瘤活性。通过乙酸乙酯萃取液体发酵桑黄菌,纯化后得到总黄酮。采用MTS法检测总黄酮的细胞活力及对小鼠成纤维细胞L929细胞及肿瘤细胞HepG-2细胞的抑制情况,并使用流式细胞仪测定不同质量浓度总黄酮(10、20、50、100μg/m L)对正常细胞及肿瘤细胞的周期影响。结果发现,药用真菌桑黄菌液体发酵所提取的不同质量浓度总黄酮(10、20、50、100μg/m L)对小鼠成纤维细胞L929细胞无抑制作用,对HepG-2细胞有直接抑制作用。液体发酵的桑黄菌落所提取的总黄酮对小鼠肿瘤有抑制作用,并且具有剂量依赖性。
To study the anti-tumor activity of flavonoids extracted from liquid fermented Phellinus igniarus,the liquid flavonoids were extracted by ethyl acetate and purified to obtain total flavonoids. MTS method was used to detect the cell viability of total flavonoids and the inhibition of mouse fibroblasts L929 cells and tumor cells HepG-2 cells. Flow cytometry was used to determine the effects of the total flavonoids in different concentrations( 10,20,50,100 μg/m L) on the cycle of normal cells and tumor cells. The results show that the different concentrations of total flavonoids( 10,20,50,100 μg/m L) extracted from liquid fermentation of Phellinus igniqrus had no inhibitory effect on mouse fibroblast L929 cells and had a direct inhibitory effect on HepG-2 cells. The total flavonoids extracted from liquid fermented Phellinus igniarius can inhibit the tumorigenesis of mice in a dose-dependent manner.
To study the anti-tumor activity of flavonoids extracted from liquid fermented Phellinus igniarus,the liquid flavonoids were extracted by ethyl acetate and purified to obtain total flavonoids. MTS method was used to detect the cell viability of total flavonoids and the inhibition of mouse fibroblasts L929 cells and tumor cells HepG-2 cells. Flow cytometry was used to determine the effects of the total flavonoids in different concentrations( 10,20,50,100 μg/m L) on the cycle of normal cells and tumor cells. The results show that the different concentrations of total flavonoids( 10,20,50,100 μg/m L) extracted from liquid fermentation of Phellinus igniqrus had no inhibitory effect on mouse fibroblast L929 cells and had a direct inhibitory effect on HepG-2 cells. The total flavonoids extracted from liquid fermented Phellinus igniarius can inhibit the tumorigenesis of mice in a dose-dependent manner.
引文
[1]吕方冰,张娜,俞淑文.桑黄多糖抗肿瘤机制研究进展[J].上海中医药杂志,2015,49(9):87-89.
[2]齐欣,张峻,陈颖,等.六种不同树种桑黄有效成分的比较[J].食品科学,2010,31(6):199-201.
[3]刘蕊.黄酮类化合物的药理作用研究进展[J].黑龙江医药,2010,23(2):234-236.
[4]昝立峰,包海鹰,李丹花,等.“桑黄”类真菌中多酚物质及其生物活性研究进展[J].天然产物研究与开发,2016(1):147-155.
[5]孙锦秀.大孔吸附树脂纯化桑黄总黄酮工艺[J].实用药物与临床,2012,15(11):744-746.
[6]许立颖,李宇,钟鸣.柚子皮多糖优化提取工艺研究[J].中医学报,2017,32(2):273-276.
[7]张敏,纪晓光,贝祝春,等.桑黄多糖抗肿瘤作用[J].中药药理与临床,2006,22(3):56-58.
[8]SHON M Y,KIM T H,SUNG N J. Antioxidants and free scavenging activity of phellinus baumii(phellinus of hymenochaetaceae)extracts[J]. Food chemistry,2003,82(4):593-597.
[9]ZHU T,KIM S H,CHEN C Y. A medicinal mushroom:Phellinus linteus[J]. Curr Med Chem,2007,15(13):1330-1335.
[10]雷萍,吴亚召,张文隽,等.桑黄菌丝体对H22荷瘤小鼠肿瘤生长的影响[J].西北大学学报(自然科学版),2015,45(5):773-776.
[11]钟石,李有贵,林天宝,等.桑黄多糖P1对肝癌Hep G2细胞周期和钙调蛋白信号通路的影响[J].中国药理学与毒理学杂志,2015,29(3):417-423.
[12]刘艳芳,杨焱,贾薇,等.药用真菌桑黄总黄酮测定方法研究[J].食用菌学报,2006,13(2):49-52.
[13]ANASTASSIADES M,LEHOTAY,S J. STAJNBAHER D,et al.,Fast and easy multiresidue method employing acetonitrile extraction/partitioning and"dispersive solid-phase extraction"for the determination of pesticide residues in produce[J]. Journal of Aoac International,2003,86(2):412-431.
[2]齐欣,张峻,陈颖,等.六种不同树种桑黄有效成分的比较[J].食品科学,2010,31(6):199-201.
[3]刘蕊.黄酮类化合物的药理作用研究进展[J].黑龙江医药,2010,23(2):234-236.
[4]昝立峰,包海鹰,李丹花,等.“桑黄”类真菌中多酚物质及其生物活性研究进展[J].天然产物研究与开发,2016(1):147-155.
[5]孙锦秀.大孔吸附树脂纯化桑黄总黄酮工艺[J].实用药物与临床,2012,15(11):744-746.
[6]许立颖,李宇,钟鸣.柚子皮多糖优化提取工艺研究[J].中医学报,2017,32(2):273-276.
[7]张敏,纪晓光,贝祝春,等.桑黄多糖抗肿瘤作用[J].中药药理与临床,2006,22(3):56-58.
[8]SHON M Y,KIM T H,SUNG N J. Antioxidants and free scavenging activity of phellinus baumii(phellinus of hymenochaetaceae)extracts[J]. Food chemistry,2003,82(4):593-597.
[9]ZHU T,KIM S H,CHEN C Y. A medicinal mushroom:Phellinus linteus[J]. Curr Med Chem,2007,15(13):1330-1335.
[10]雷萍,吴亚召,张文隽,等.桑黄菌丝体对H22荷瘤小鼠肿瘤生长的影响[J].西北大学学报(自然科学版),2015,45(5):773-776.
[11]钟石,李有贵,林天宝,等.桑黄多糖P1对肝癌Hep G2细胞周期和钙调蛋白信号通路的影响[J].中国药理学与毒理学杂志,2015,29(3):417-423.
[12]刘艳芳,杨焱,贾薇,等.药用真菌桑黄总黄酮测定方法研究[J].食用菌学报,2006,13(2):49-52.
[13]ANASTASSIADES M,LEHOTAY,S J. STAJNBAHER D,et al.,Fast and easy multiresidue method employing acetonitrile extraction/partitioning and"dispersive solid-phase extraction"for the determination of pesticide residues in produce[J]. Journal of Aoac International,2003,86(2):412-431.