γ-H2AX在辐射生物剂量估算中的研究进展
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摘要
γ-H2AX对受照人员DNA双链断裂损伤修复具有重要作用,利用γ-H2AX焦点计数可以估算照射剂量,因其检测周期短、特异性与灵敏度相对较高,近几年被国内外广泛应用于辐射剂量生物估算研究。本文对γ-H2AX在生物剂量估算中的应用与研究进展做一综述,对其在核与辐射事故批量受照人员的快速剂量估算以及伤员分类的应用前景上进行展望。
γ-H2 AX plays a key role in the DNA double-strand break and repair for exposed individuals. Estimating the radiation dose using γ-H2 AX foci count,which has short detection cycle,high specificity,and sensitivity,has been widely applied in the research of radiation biodosimetry estimation both in domestic and abroad in recent years. The review focuses on the research progress in the application of γ-H2 AX in radiation biodosimetry estimation,as well as the rapid dose estimation received in nuclear radiation accident of batch exposed individuals and radiation triage.
γ-H2 AX plays a key role in the DNA double-strand break and repair for exposed individuals. Estimating the radiation dose using γ-H2 AX foci count,which has short detection cycle,high specificity,and sensitivity,has been widely applied in the research of radiation biodosimetry estimation both in domestic and abroad in recent years. The review focuses on the research progress in the application of γ-H2 AX in radiation biodosimetry estimation,as well as the rapid dose estimation received in nuclear radiation accident of batch exposed individuals and radiation triage.
引文
[1]International Atomic Energy Agency,World Health Organization.Cytogenetic dosimetry:applications in preparedness for and response to radiation emergencies[M].Vienna:International Atomic Energy Agency,2011.
[2]Valdiglesias V,Giunta S,Fenech M,et al.γH2AX as a marker of DNA double Strand breaks and genomic instability in human population studies[J].Mutation research,2013,753(1):24-40.
[3]Redon CE,Dickey JS,Bonner WM,et al.γ-H2AX as a biomarker of DNA damage induced by ionizing radiation in human peripheral blood lymphocytes and artificial skin[J].Advances in space research:the official journal of the Committee on Space Research(COSPAR),2009,43(8):1171-1178.
[4]Tu WZ,Li B,Huang B,et al.γH2AX foci formation in the absence of DNA damage:mitotic H2AX phosphorylation is mediated by the DNA-PKcs/CHK2 pathway[J].FEBS Letters,2013,587(21):3437-3443.
[5]Giunta S,Jackson SP.Give me a break,but not in mitosis:the mitotic DNA damage response marks DNA double-strand breaks with early signaling events[J].Cell cycle(Georgetown,Tex.),2011,10(8):1215-1221.
[6]Mah LJ,El-Osta A,Karagiannis TC.Gamma H2AX as a molecular marker of aging and disease[J].Epigenetics,2010,5(2):129-136.
[7]Ishida M,Ishida T,Tashiro S,et al.Smoking cessation reverses DNA double-strand breaks in human mononuclear cells[J].Plo S One,2014,9(8):e103993.
[8]Li Z,Yang J,Huang H.Oxidative stress induces H2AX phosphorylation in human spermatozoa[J].FEBS Letters,2006,580(26):6161-6168.
[9]Ismail IH,Wadhra TI,Hammarsten O.An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans[J].Nucleic Acids Research,2007,35(5):e36.
[10]Sharma PM,Ponnaiya B,Taveras M,et al.High throughput measurement ofγH2AX DSB repair kinetics in a healthy human population[J].Plo S One,2015,10(3):e0121083.
[11]Sedelnikova OA,Horikawa I,Redon C,et al.Delayed kinetics of DNA double-strand break processing in normal and pathological aging[J].Aging Cell,2008,7(1):89-100.
[12]Turner HC,Sharma P,Perrier JR,et al.The RABi T:highthroughput technology for assessing global DSB repair[J].Radiation and Environmental Biophysics,2014,53(2):265-272.
[13]Rothkamm K,Horn S,Scherthan H,et al.Laboratory intercomparison on theγ-H2AX foci assay[J].Radiation Research,2013,180(2):149-155.
[14]Redon CE,Nakamura AJ,Sordet O,et al.γ-H2AX detection in peripheral blood lymphocytes,splenocytes,bone marrow,xenografts,and skin[J].Methods in molecular biology(Clifton,N.J.),2011,682:249-270.
[15]Muslimovic A,Ismail IH,Gao Y,et al.An optimized method for measurement of gamma-H2AX in blood mononuclear and cultured cells[J].Nature Protocols,2008,3(7):1187-1193.
[16]Horn S,Barnard S,Rothkamm K.Gamma-H2AX-based dose estimation for whole and partial body radiation exposure[J].Plo S One,2011,6(9):e25113.
[17]Rothkamm K,Barnard S,Ainsbury EA,et al.Manual versus automatedγ-H2AX foci analysis across five European laboratories:can this assay be used for rapid biodosimetry in a large scale radiation accident?[J].Mutation research,2013,756(1/2):170-173.
[18]Barnard S,Ainsbury EA,Al-Hafidh J,et al.The first gammaH2AX biodosimetry intercomparison exercise of the developing European biodosimetry network RENEB[J].Radiation Protection Dosimetry,2015,164(3):265-270.
[19]Tanaka T,Halicka D,Traganos F,et al.Cytometric analysis of DNA damage:phosphorylation of histone H2AX as a marker of DNA double-strand breaks(DSBs)[J].Methods in molecular biology(Clifton,N.J.),2009,523:161-168.
[20]Moquet J,Barnard S,Rothkamm K.Gamma-H2AX biodosimetry for use in large scale radiation incidents:comparison of a rapid'96well lyse/fix'protocol with a routine method[J].Peer J,2014,2:e282.
[21]Barber PR,Locke RJ,Pierce GP,et al.Gamma-H2AX Foci counting:image processing and control software for high-content screening-art.no.64411M[C]//Proceedings of SPIE,6441,2007:M4411.
[22]胡海亮,王治东,张学清,等.用流式细胞术检测γ射线诱发淋巴细胞γ-H2AX与照射剂量的关系[J].辐射研究与辐射工艺学报,2012,30(2):120-124.
[23]Nakamura A,Sedelnikova OA,Redon C,et al.Techniques for gamma-H2AX detection[J].Methods in enzymology,2006,409:236-250.
[24]凌攀,刘毅,蹇启政,等.从放人员染色体、微核检查的制片方法改进探讨[J].现代预防医学,2015,42(6):1096-1098.
[25]信亚周,马娅,刘伟.早熟染色体凝集(PCC)在辐射生物剂量估算中的研究进展[J].中国辐射卫生,2012,21(2):251-253.
[26]李秀芹,赵进沛,任庆余,等.GPA基因突变技术及其在放射生物剂量估计中的应用[J].现代预防医学,2007,34(1):69-70,75.
[27]宋秀军,何蕊,江其生.核事故中辐射生物剂量计的应用(文献综述)[J].放射免疫学杂志,2012,25(1):29-31.
[2]Valdiglesias V,Giunta S,Fenech M,et al.γH2AX as a marker of DNA double Strand breaks and genomic instability in human population studies[J].Mutation research,2013,753(1):24-40.
[3]Redon CE,Dickey JS,Bonner WM,et al.γ-H2AX as a biomarker of DNA damage induced by ionizing radiation in human peripheral blood lymphocytes and artificial skin[J].Advances in space research:the official journal of the Committee on Space Research(COSPAR),2009,43(8):1171-1178.
[4]Tu WZ,Li B,Huang B,et al.γH2AX foci formation in the absence of DNA damage:mitotic H2AX phosphorylation is mediated by the DNA-PKcs/CHK2 pathway[J].FEBS Letters,2013,587(21):3437-3443.
[5]Giunta S,Jackson SP.Give me a break,but not in mitosis:the mitotic DNA damage response marks DNA double-strand breaks with early signaling events[J].Cell cycle(Georgetown,Tex.),2011,10(8):1215-1221.
[6]Mah LJ,El-Osta A,Karagiannis TC.Gamma H2AX as a molecular marker of aging and disease[J].Epigenetics,2010,5(2):129-136.
[7]Ishida M,Ishida T,Tashiro S,et al.Smoking cessation reverses DNA double-strand breaks in human mononuclear cells[J].Plo S One,2014,9(8):e103993.
[8]Li Z,Yang J,Huang H.Oxidative stress induces H2AX phosphorylation in human spermatozoa[J].FEBS Letters,2006,580(26):6161-6168.
[9]Ismail IH,Wadhra TI,Hammarsten O.An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans[J].Nucleic Acids Research,2007,35(5):e36.
[10]Sharma PM,Ponnaiya B,Taveras M,et al.High throughput measurement ofγH2AX DSB repair kinetics in a healthy human population[J].Plo S One,2015,10(3):e0121083.
[11]Sedelnikova OA,Horikawa I,Redon C,et al.Delayed kinetics of DNA double-strand break processing in normal and pathological aging[J].Aging Cell,2008,7(1):89-100.
[12]Turner HC,Sharma P,Perrier JR,et al.The RABi T:highthroughput technology for assessing global DSB repair[J].Radiation and Environmental Biophysics,2014,53(2):265-272.
[13]Rothkamm K,Horn S,Scherthan H,et al.Laboratory intercomparison on theγ-H2AX foci assay[J].Radiation Research,2013,180(2):149-155.
[14]Redon CE,Nakamura AJ,Sordet O,et al.γ-H2AX detection in peripheral blood lymphocytes,splenocytes,bone marrow,xenografts,and skin[J].Methods in molecular biology(Clifton,N.J.),2011,682:249-270.
[15]Muslimovic A,Ismail IH,Gao Y,et al.An optimized method for measurement of gamma-H2AX in blood mononuclear and cultured cells[J].Nature Protocols,2008,3(7):1187-1193.
[16]Horn S,Barnard S,Rothkamm K.Gamma-H2AX-based dose estimation for whole and partial body radiation exposure[J].Plo S One,2011,6(9):e25113.
[17]Rothkamm K,Barnard S,Ainsbury EA,et al.Manual versus automatedγ-H2AX foci analysis across five European laboratories:can this assay be used for rapid biodosimetry in a large scale radiation accident?[J].Mutation research,2013,756(1/2):170-173.
[18]Barnard S,Ainsbury EA,Al-Hafidh J,et al.The first gammaH2AX biodosimetry intercomparison exercise of the developing European biodosimetry network RENEB[J].Radiation Protection Dosimetry,2015,164(3):265-270.
[19]Tanaka T,Halicka D,Traganos F,et al.Cytometric analysis of DNA damage:phosphorylation of histone H2AX as a marker of DNA double-strand breaks(DSBs)[J].Methods in molecular biology(Clifton,N.J.),2009,523:161-168.
[20]Moquet J,Barnard S,Rothkamm K.Gamma-H2AX biodosimetry for use in large scale radiation incidents:comparison of a rapid'96well lyse/fix'protocol with a routine method[J].Peer J,2014,2:e282.
[21]Barber PR,Locke RJ,Pierce GP,et al.Gamma-H2AX Foci counting:image processing and control software for high-content screening-art.no.64411M[C]//Proceedings of SPIE,6441,2007:M4411.
[22]胡海亮,王治东,张学清,等.用流式细胞术检测γ射线诱发淋巴细胞γ-H2AX与照射剂量的关系[J].辐射研究与辐射工艺学报,2012,30(2):120-124.
[23]Nakamura A,Sedelnikova OA,Redon C,et al.Techniques for gamma-H2AX detection[J].Methods in enzymology,2006,409:236-250.
[24]凌攀,刘毅,蹇启政,等.从放人员染色体、微核检查的制片方法改进探讨[J].现代预防医学,2015,42(6):1096-1098.
[25]信亚周,马娅,刘伟.早熟染色体凝集(PCC)在辐射生物剂量估算中的研究进展[J].中国辐射卫生,2012,21(2):251-253.
[26]李秀芹,赵进沛,任庆余,等.GPA基因突变技术及其在放射生物剂量估计中的应用[J].现代预防医学,2007,34(1):69-70,75.
[27]宋秀军,何蕊,江其生.核事故中辐射生物剂量计的应用(文献综述)[J].放射免疫学杂志,2012,25(1):29-31.