倒地铃两种单体成分α-糖苷酶抑制活性研究
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摘要
目的:以倒地铃单体成分蒲公英赛醇、3β-赤杨醇为研究对象,对其体外α-葡萄糖苷酶抑制活性进行筛选。方法:应用体外α-葡萄糖苷酶抑制模型对倒地铃两种单体成分进行α-糖苷酶抑制活力的测定。结果:3β-赤杨醇、蒲公英赛醇对α-糖苷酶均具有较高的抑制活性,其IC_(50)值分别为0.75mg/mL和0.82mg/mL。结论:倒地铃两种单体成分均具有较好的α-葡萄糖苷酶抑制活性,具有广泛的应用前景和较大的开发价值。
Objective:To screenα-Glucosidase inhibitory activity of 3β-hydroxyglutin-5-ene and taraxerol isolated fromCardiospermum halicacabum L.Methods:The inhibitory activity of these two compounds for theα-glucosidase were determined by in vitro experiments.Results:These two compounds were all showed high inhibitory activity at the same time and close to acarbose(IC_(50)=0.80 mg/mL)to a certain degree,the IC_(50) of which was 0.75 mg/mL and 0.82 mg/mL,respectively.Conclusion:Two compositions fromCardiospermum halicacabum L.were all showedα-Glucosidase inhibitory,which had a great range of potential utilities and a prospect of development.
Objective:To screenα-Glucosidase inhibitory activity of 3β-hydroxyglutin-5-ene and taraxerol isolated fromCardiospermum halicacabum L.Methods:The inhibitory activity of these two compounds for theα-glucosidase were determined by in vitro experiments.Results:These two compounds were all showed high inhibitory activity at the same time and close to acarbose(IC_(50)=0.80 mg/mL)to a certain degree,the IC_(50) of which was 0.75 mg/mL and 0.82 mg/mL,respectively.Conclusion:Two compositions fromCardiospermum halicacabum L.were all showedα-Glucosidase inhibitory,which had a great range of potential utilities and a prospect of development.
引文
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[6]屠洁,刘冠卉,李前龙.麦麸皮提取物对α-葡萄糖苷酶的抑制活性研究[J].食品研究与开发,2010,31(8):44-47.
[7]CHUN W C,YEON H C,CHA M R.Alpha-glucosidase inhibitor from seed extract of paeonia lactiflora[J].J Korean Soc Appl Biol Chem,2009(6):638-642.
[2]《中华本草》编委会.中华本草[M].第1版.上海:上海人民出版社,1999:3969-3970.
[3]陈君,韦建华,苗伟生,等.倒地铃急性毒性及降血糖作用的初步研究[J].时珍国医国药,2012,23(5):1083-1085.
[4]韦建华,陈君,蔡少芳,等.倒地铃化学成分研究(I)[J].中草药,2011,42(8):1509-1511.
[4]李玉萍,白冰,叶军,等.α-葡萄糖苷酶抑制剂的制备和活性研究进展[J].食品科学,2008,29(9):617-620.
[6]屠洁,刘冠卉,李前龙.麦麸皮提取物对α-葡萄糖苷酶的抑制活性研究[J].食品研究与开发,2010,31(8):44-47.
[7]CHUN W C,YEON H C,CHA M R.Alpha-glucosidase inhibitor from seed extract of paeonia lactiflora[J].J Korean Soc Appl Biol Chem,2009(6):638-642.