昼夜节律紊乱对大鼠运动耐力、运动后血压和心率的影响
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摘要
目的观察昼夜节律紊乱对大鼠运动耐力的影响。方法自发性高血压大鼠(SHR)30只和与其年龄、体质量相匹配的同源正常血压(WKY)大鼠30只,随机分为正常节律组(对照组)、12 h节律紊乱组(观察组1)、6 h节律紊乱组(观察组2),每组SHR及WKY大鼠各10只,通过改变光照/黑暗时间构建节律紊乱模型。造模前及造模后1个月、2个月和3个月分别进行一次性力竭运动试验,力竭运动后测量其血压、心率。结果 SHR大鼠造模时间与昼夜节律对力竭时间存在交互作用(F=2.409,P=0.047),造模3个月后,对照组力竭时间高于观察组1和观察组2(F=4.290,P=0.032);WKY大鼠造模时间与昼夜节律对力竭时间无交互作用(F=0.717,P=0.638),不同组间力竭时间无显著性差异(P>0.05)。造模3个月后,SHR大鼠观察组1运动后收缩压、舒张压、平均动脉压均高于对照组和观察组2(P<0.05),观察组1和观察组2运动后心率高于对照组(P<0.05);WKY大鼠不同组间运动后收缩压、舒张压、平均动脉压及心率均无显著性差异(P>0.05)。结论昼夜节律紊乱会降低SHR大鼠的运动耐力,可能与大鼠的心血管功能损伤有关。
Objective To observe the influence of circadian rhythm disruptions on exercise capability in rats.Methods A total of 30 spontaneously hypertensive rats(SHR) and age and weight matched homologous normal blood pressure WKY rats were randomly divided into normal circadian rhythm group(control group), twelve hours circadian rhythm disruption group(observation group 1), six hours circadian rhythm disruption group(observation group 2). Each group included ten SHR rats and WKY rats, changing the light/dark time to construct circadian rhythm disruption model. Exercise endurance, blood pressure and heart rate after exhaustion were measured before and one, two and three months after modeling.Results The interaction of time and circadian rhythm was significant on the exhaustion time of SHR rats(F=2.409, P=0.047). After three months of modeling, the exhaustion time of the control group was more than that of the observation groups 1 and 2(F=4.290, P=0.032). The interaction of time and circadian rhythm was not significant on the exhaustion time of WKY rats(F=0.717, P=0.638), there was no significant difference in the exhaustion time among groups(P>0.05). For SHR rats, after three months of modeling, the systolic pressure, diastolic pressure and mean arterial pressure after exhaustion of the observation group 1 were higher than those of the control group and the observation group 2(P<0.05), the heart rate of the observation groups 1 and 2 was higher than that of the control group(P<0.05). But there was no significant difference in the systolic pressure, diastolic pressure,average arterial pressure and heart rate of WKY rats among groups(P>0.05).Conclusion Circadian rhythm disruptions can reduce the exercise endurance of SHR rats, which may be related to the cardiovascular function impairment.
Objective To observe the influence of circadian rhythm disruptions on exercise capability in rats.Methods A total of 30 spontaneously hypertensive rats(SHR) and age and weight matched homologous normal blood pressure WKY rats were randomly divided into normal circadian rhythm group(control group), twelve hours circadian rhythm disruption group(observation group 1), six hours circadian rhythm disruption group(observation group 2). Each group included ten SHR rats and WKY rats, changing the light/dark time to construct circadian rhythm disruption model. Exercise endurance, blood pressure and heart rate after exhaustion were measured before and one, two and three months after modeling.Results The interaction of time and circadian rhythm was significant on the exhaustion time of SHR rats(F=2.409, P=0.047). After three months of modeling, the exhaustion time of the control group was more than that of the observation groups 1 and 2(F=4.290, P=0.032). The interaction of time and circadian rhythm was not significant on the exhaustion time of WKY rats(F=0.717, P=0.638), there was no significant difference in the exhaustion time among groups(P>0.05). For SHR rats, after three months of modeling, the systolic pressure, diastolic pressure and mean arterial pressure after exhaustion of the observation group 1 were higher than those of the control group and the observation group 2(P<0.05), the heart rate of the observation groups 1 and 2 was higher than that of the control group(P<0.05). But there was no significant difference in the systolic pressure, diastolic pressure,average arterial pressure and heart rate of WKY rats among groups(P>0.05).Conclusion Circadian rhythm disruptions can reduce the exercise endurance of SHR rats, which may be related to the cardiovascular function impairment.
引文
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[28]Carpagnano GE,Lacedonia D,Malerba M,et al.Exhaled breath temperature measurement:influence of circadian rhythm[J].J Biol Regul Homeost Agents,2017,31(1):229-235.
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[30]Vaara J,Kyrolainen H,Koivu M,et al.The effect of 60-h sleep deprivation on cardiovascular regulation and body temperature[J].Eur J Appl Physiol,2009,105(3):439-444.
[31]Sundar IK,Yao H,Sellix MT,et al.Circadian clock-coupled lung cellular and molecular functions in chronic airway diseases[J].Am J Respir Cell Mol Biol,2015,53(3):285-290.
[32]Vetter C,Devore EE,Wegrzyn LR,et al.Association between rotating night shift work and risk of coronary heart disease among women[J].JAMA,2016,315(16):1726-1734.
[33]Herdy AH,Ritt LE,Stein R,et al.Cardiopulmonary exercise test:background,applicability and interpretation[J].Arq Bras Cardiol,2016,107(5):467-481.
[34]van Moorsel D,Hansen J,Havekes B,et al.Demonstration of a day-night rhythm in human skeletal muscle oxidative capacity[J].Mol Metab,2016,5(8):635-645.
[35]Aoyama S,Shibata S.The role of circadian rhythms in muscular and osseous physiology and their regulation by nutrition and exercise[J].Front Neurosci,2017,2(11):63.
[36]Schroder EA,Harfmann BD,Zhang X,et al.Intrinsic muscle clock is necessary for musculoskeletal health[J].J Physiol,2015,593(24):5387-5404.
[37]Dyar KA,Ciciliot S,Wright LE,et al.Muscle insulin sensitivity and glucose metabolism are controlled by the intrinsic muscle clock[J].Mol Metab,2014,3(1):29-41.
[38]Andrews JL,Zhang X,Mc Carthy JJ,et al.CLOCK and BMAL1 regulate Myo D and are necessary for maintenance of skeletal muscle phenotype and function[J].Proc Natl Acad Sci U S A,2010,107(44):19090-19095.
[39]Dela PI,Dela PI,de la Pena JB,et al.Methylphenidate and atomoxetine-responsive prefrontal cortical genetic overlaps in"impulsive"SHR/NCrl and Wistar rats[J].Behav Genet,2017,47(5):564-580.
[40]Olejnikova L,Polidarova L,Pauslyova L,et al.Diverse development and higher sensitivity of the circadian clocks to changes in maternal-feeding regime in a rat model of cardio-metabolic disease[J].Chronobiol Int,2015,32(4):531-547.
[41]韦汐,胡世红.运动血压异常与心血管疾病的关系[J].重庆医学,2012,41(21):2214-2216.
[42]于宗良.运动后收缩压异常升高对冠心病的诊断价值的初步探讨[D].苏州:苏州大学,2003.
[43]杨兴义,胡志刚,赵勇,等.血管内皮功能与运动后血压反应相关性的研究[J].临床心血管病杂志,2005,21(2):76-78.
[44]康美华,王成.运动后心率恢复的研究进展[J].中国循证儿科杂志,2014,9(1):72-76.
[45]Portaluppi F,Tiseo R,Smolensky MH,et al.Circadian rhythms and cardiovascular health[J].Sleep Med Rev,2012,16(2):151-166.
[46]Kamada Y,Masuda T,Tanaka S,et al.Muscle weakness is associated with an increase of left ventricular mass through excessive blood pressure elevation during exercise in patients with hypertension[J].Int Heart J,2017,58(4):551-556.
[47]Gomes AP,Doederlein PM.A review on post-exercise hypotension in hypertensive individuals[J].Arq Bras Cardiol,2011,96(5):100-109.
[2]袁艳鹏.昼夜节律紊乱与心脑血管疾病[J].国际心血管病杂志,2010,37(5):292-294.
[3]Riley LA,Esser KA.The role of the molecular clock in skeletal muscle and what it is teaching us about muscle-bone crosstalk[J].Curr Osteoporos Rep,2017,15(3):222-230.
[4]Herrero L,Valcarcel L,Da SC,et al.Altered circadian rhythm and metabolic gene profile in rats subjected to advanced light phase shifts[J].PLo S One,2015,10(4):e122570.
[5]Portaluppi F,Tiseo R,Smolensky MH,et al.Circadian rhythms and cardiovascular health[J].Sleep Med Rev,2012,16(2):151-166.
[6]Boivin DB,Boudreau P.Impacts of shift work on sleep and circadian rhythms[J].Pathol Biol(Paris),2014,62(5):292-301.
[7]Sridhar GR,Sanjana NS.Sleep,circadian dysrhythmia,obesity and diabetes[J].World J Diabetes,2016,7(19):515-522.
[8]赵冬云,丁思悦,郭丽华,等.医院倒班制临床从业群体职业应激现况及对心血管危险因素的影响分析[J].中国医刊,2017,52(3):36-39.
[9]Samulin EJ,Noto HO,Skare O,et al.Mechanisms of breast cancer risk in shift workers:association of telomere shortening with the duration and intensity of night work[J].Cancer Med,2017,6(8):1988-1997.
[10]Barker LM,Nussbaum MA.Fatigue,performance and the work environment:a survey of registered nurses[J].J Adv Nurs,2011,67(6):1370-1382.
[11]Moradifar R,Hoveidi H,Givehchi S,et al.Examining fatigue and insomnia symptoms among workers of a gas transmission industry in 2013[J].Electron Physician,2014,6(2):827-831.
[12]Stimpfel AW,Brewer CS,Kovner CT.Scheduling and shift work characteristics associated with risk for occupational injury in newly licensed registered nurses:An observational study[J].Int J Nurs Stud,2015,52(11):1686-1693.
[13]Dall'Ora C,Ball J,Recio-Saucedo A,et al.Characteristics of shift work and their impact on employee performance and wellbeing:A literature review[J].Int J Nurs Stud,2016,57(5):12-27.
[14]O'Leary TJ,Collett J,Howells K,et al.Endurance capacity and neuromuscular fatigue following high-vs moderate-intensity endurance training:A randomized trial[J].Scand J Med Sci Sports,2017,16(2):1111.
[15]罗雪琚.从普通病例谈高血压发病率的攀升[J].中华高血压杂志,2016,24(5):402.
[16]李镒冲,王丽敏,姜勇,等.2010年中国成年人高血压患病情况[J].中华预防医学杂志,2012,46(5):409-413.
[17]Missault LH,Duprez DA,Brandt AA,et al.Exercise performance and diastolic filling in essential hypertension[J].Blood Press,1993,2(4):284-288.
[18]Patrick Y,Lee A,Raha O,et al.Effects of sleep deprivation on cognitive and physical performance in university students[J].Sleep Biol Rhythms,2017,15(3):217-225.
[19]Mejri MA,Yousfi N,Mhenni T,et al.Does one night of partial sleep deprivation affect the evening performance during intermittent exercise in Taekwondo players?[J].J Exerc Rehabil,2016,12(1):47-53.
[20]Mejri MA,Yousfi N,Hammouda O,et al.One night of partial sleep deprivation increased biomarkers of muscle and cardiac injuries during acute intermittent exercise[J].J Sports Med Phys Fitness,2017,57(5):643-651.
[21]Xin L,Sun X,Lou S.Effects of methane-rich saline on the capability of one-time exhaustive exercise in male SD rats[J].PLo S One,2016,11(3):e150925.
[22]Matsumura T,Nakagawa H,Suzuki K,et al.Influence of circadian disruption on neurotransmitter levels,physiological indexes,and behaviour in rats[J].Chronobiol Int,2015,32(10):1449-1457.
[23]Bessot N,Moussay S,Gauthier A,et al.Effect of pedal rate on diurnal variations in cardiorespiratory variables[J].Chronobiol Int,2006,23(4):877-887.
[24]Hamouda O,Chtourou H,Farjallah MA,et al.The effect of Ramadan fasting on the diurnal variations in aerobic and anaerobic performances in Tunisian youth soccer players[J].Biological Rhythm Research,2012,43(2):177-190.
[25]Harfmann BD,Schroder EA,Esser KA.Circadian rhythms,the molecular clock,and skeletal muscle[J].J Biol Rhythms,2015,30(2):84-94.
[26]Sundar IK,Yao H,Sellix MT,et al.Circadian molecular clock in lung pathophysiology[J].Am J Physiol Lung Cell Mol Physiol,2015,309(10):1056-1075.
[27]Gubin DG,Weinert D,Rybina SV,et al.Activity,sleep and ambient light have a different impact on circadian blood pressure,heart rate and body temperature rhythms[J].Chronobiol Int,2017,34(5):632-649.
[28]Carpagnano GE,Lacedonia D,Malerba M,et al.Exhaled breath temperature measurement:influence of circadian rhythm[J].J Biol Regul Homeost Agents,2017,31(1):229-235.
[29]Degaute JP,van de Borne P,Linkowski P,et al.Quantitative analysis of the 24-hour blood pressure and heart rate patterns in young men[J].Hypertension,1991,18(2):199-210.
[30]Vaara J,Kyrolainen H,Koivu M,et al.The effect of 60-h sleep deprivation on cardiovascular regulation and body temperature[J].Eur J Appl Physiol,2009,105(3):439-444.
[31]Sundar IK,Yao H,Sellix MT,et al.Circadian clock-coupled lung cellular and molecular functions in chronic airway diseases[J].Am J Respir Cell Mol Biol,2015,53(3):285-290.
[32]Vetter C,Devore EE,Wegrzyn LR,et al.Association between rotating night shift work and risk of coronary heart disease among women[J].JAMA,2016,315(16):1726-1734.
[33]Herdy AH,Ritt LE,Stein R,et al.Cardiopulmonary exercise test:background,applicability and interpretation[J].Arq Bras Cardiol,2016,107(5):467-481.
[34]van Moorsel D,Hansen J,Havekes B,et al.Demonstration of a day-night rhythm in human skeletal muscle oxidative capacity[J].Mol Metab,2016,5(8):635-645.
[35]Aoyama S,Shibata S.The role of circadian rhythms in muscular and osseous physiology and their regulation by nutrition and exercise[J].Front Neurosci,2017,2(11):63.
[36]Schroder EA,Harfmann BD,Zhang X,et al.Intrinsic muscle clock is necessary for musculoskeletal health[J].J Physiol,2015,593(24):5387-5404.
[37]Dyar KA,Ciciliot S,Wright LE,et al.Muscle insulin sensitivity and glucose metabolism are controlled by the intrinsic muscle clock[J].Mol Metab,2014,3(1):29-41.
[38]Andrews JL,Zhang X,Mc Carthy JJ,et al.CLOCK and BMAL1 regulate Myo D and are necessary for maintenance of skeletal muscle phenotype and function[J].Proc Natl Acad Sci U S A,2010,107(44):19090-19095.
[39]Dela PI,Dela PI,de la Pena JB,et al.Methylphenidate and atomoxetine-responsive prefrontal cortical genetic overlaps in"impulsive"SHR/NCrl and Wistar rats[J].Behav Genet,2017,47(5):564-580.
[40]Olejnikova L,Polidarova L,Pauslyova L,et al.Diverse development and higher sensitivity of the circadian clocks to changes in maternal-feeding regime in a rat model of cardio-metabolic disease[J].Chronobiol Int,2015,32(4):531-547.
[41]韦汐,胡世红.运动血压异常与心血管疾病的关系[J].重庆医学,2012,41(21):2214-2216.
[42]于宗良.运动后收缩压异常升高对冠心病的诊断价值的初步探讨[D].苏州:苏州大学,2003.
[43]杨兴义,胡志刚,赵勇,等.血管内皮功能与运动后血压反应相关性的研究[J].临床心血管病杂志,2005,21(2):76-78.
[44]康美华,王成.运动后心率恢复的研究进展[J].中国循证儿科杂志,2014,9(1):72-76.
[45]Portaluppi F,Tiseo R,Smolensky MH,et al.Circadian rhythms and cardiovascular health[J].Sleep Med Rev,2012,16(2):151-166.
[46]Kamada Y,Masuda T,Tanaka S,et al.Muscle weakness is associated with an increase of left ventricular mass through excessive blood pressure elevation during exercise in patients with hypertension[J].Int Heart J,2017,58(4):551-556.
[47]Gomes AP,Doederlein PM.A review on post-exercise hypotension in hypertensive individuals[J].Arq Bras Cardiol,2011,96(5):100-109.