陆眠宁Ⅱ麻醉对家兔酸碱平衡重要指标的影响
|
摘要
目的比较陆眠宁Ⅱ、乌拉坦全身麻醉对家兔酸碱平衡重要指标的影响。方法成年健康新西兰大白兔40只,雌雄各半,随机分为2组,分别为陆眠宁Ⅱ组和乌拉坦组。陆眠宁Ⅱ组采用低剂量多频次肌注给药:总剂量0.3~0.4 mL·kg~(-1)。乌拉坦组采用质量分数25%乌拉坦按5 mL·kg~(-1)耳缘静脉给药,麻醉前耳缘动脉采血测定血气指标,麻醉后观察显效时间、维持时间及动物死亡情况,股动脉采血测定血气指标。比较两种麻醉药对酸碱平衡重要指标pH、碱剩余(BE)、二氧化碳分压(PaCO_2)及HCO_3~-的影响。结果陆眠宁Ⅱ有较好的镇静麻醉效果,呼吸抑制稍明显,但不易造成动物死亡。陆眠宁Ⅱ麻醉后家兔pH(7.35±0.04),BE(6.8±3.49)mmol·L~(-1),PaCO_2(58.32±8.45)mm Hg,HCO_3~-(32±3.02)mmol·L~(-1);乌拉坦麻醉后家兔pH(7.36±0.04),BE(-5.8±3.12)mmol·L~(-1),PaCO_2(33.44±7.05)mm Hg,HCO_3~-(16.23±3.15)mmol·L~(-1),陆眠宁Ⅱ组与正常对照相比,pH无明显差异(P>0.05),BE、PaCO_2及HCO_3~-显著高于正常组(P<0.01);乌拉坦组与正常对照相比,各项指标均无显著性差异(P>0.05)。结论本研究获得了陆眠宁Ⅱ麻醉对家兔酸碱平衡重要指标影响的实验资料,为实验教学、科研及兽医临床提供参考。
Objective To compare the effects on major indexes of acid-base balance between Lu MianningⅡand 25% urethane.Methods A total of 40 adult healthy New Zealand white rabbits with half male and half female were randomly divided into two groups,Lu Mianning Ⅱ and urethane.As control,before anesthesia,blood samples were collected from auricular arteria for blood gas analysis. Twenty of them were intravenously injected with 5 mL·kg~(-1) of 25% urethane,and twenty rabbits were given intramuscular injection of Lu MianningⅡ by multiple low-dosing: the total dose was 0. 3-0. 4 mL·kg~(-1).After the general anesthesia, the acid-base balance disorder experiment was carried out.The onset time,duration time and the death of animals were observed.The differences of pH,BE,HCO_3~- and PaCO_2 in the acid-base balance test were compared. Results Lu Mianning Ⅱ had a good effect of sedation anesthesia,and the respiratory inhibition was obvious,while it was not easy to cause the death of the animals. After Lu Mianning Ⅱanesthesia, the pH,BE,HCO_3~- and PaCO_2 were( 7. 35±0. 04),( 6. 8±3. 49) mmol·L~(-1),( 32±3. 02) mmol·L~(-1),and( 58. 32 ± 8. 45) mmHg; After urethane anesthesia, the pH,BE,HCO_3~- and PaCO_2 were( 7. 36 ±0. 04),(-5. 8±3. 12) mmol·L~(-1),( 16. 23±3. 15) mmol·L~(-1),and( 33. 44±7. 05) mmHg.Compared with control, there was no significant difference in pH( P>0. 05),while significant increases were observed in PaCO_2,BE and HCO_3~-( P < 0. 01) of Lu MianningⅡ group. All indexes of urethane have no difference compared to control( P>0. 05).Conclusion This study obtained experimental data of major indexes on acid base balance experiment in rabbits,which provide reference for experimental teaching, scientific research and veterinary clinic.
Objective To compare the effects on major indexes of acid-base balance between Lu MianningⅡand 25% urethane.Methods A total of 40 adult healthy New Zealand white rabbits with half male and half female were randomly divided into two groups,Lu Mianning Ⅱ and urethane.As control,before anesthesia,blood samples were collected from auricular arteria for blood gas analysis. Twenty of them were intravenously injected with 5 mL·kg~(-1) of 25% urethane,and twenty rabbits were given intramuscular injection of Lu MianningⅡ by multiple low-dosing: the total dose was 0. 3-0. 4 mL·kg~(-1).After the general anesthesia, the acid-base balance disorder experiment was carried out.The onset time,duration time and the death of animals were observed.The differences of pH,BE,HCO_3~- and PaCO_2 in the acid-base balance test were compared. Results Lu Mianning Ⅱ had a good effect of sedation anesthesia,and the respiratory inhibition was obvious,while it was not easy to cause the death of the animals. After Lu Mianning Ⅱanesthesia, the pH,BE,HCO_3~- and PaCO_2 were( 7. 35±0. 04),( 6. 8±3. 49) mmol·L~(-1),( 32±3. 02) mmol·L~(-1),and( 58. 32 ± 8. 45) mmHg; After urethane anesthesia, the pH,BE,HCO_3~- and PaCO_2 were( 7. 36 ±0. 04),(-5. 8±3. 12) mmol·L~(-1),( 16. 23±3. 15) mmol·L~(-1),and( 33. 44±7. 05) mmHg.Compared with control, there was no significant difference in pH( P>0. 05),while significant increases were observed in PaCO_2,BE and HCO_3~-( P < 0. 01) of Lu MianningⅡ group. All indexes of urethane have no difference compared to control( P>0. 05).Conclusion This study obtained experimental data of major indexes on acid base balance experiment in rabbits,which provide reference for experimental teaching, scientific research and veterinary clinic.
引文
[1]王卫,王万铁.门静脉阻断及再开放对家兔酸碱平衡的影响[J].肝胆胰外科杂志,1997,9(3):137-140.
[2]HA C W,PARK Y B,CHUNG J Y,et al.Cartilage repair using composites of human umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel in a minipig model[J].Stem Cells Translational M edicine,2015,4(9):342-347.
[3]ZHANG H,CHAI M,LIU C,et al.Endoplasmic reticulum is involved in myocardial injury in a miniature sw ine model of coronary artery stenosis exposed to acceleration—associated stress[J].Plos One,2014,10(7):541-548.
[4]陈小波,简道林.腹腔复苏对失血性休克家兔血乳酸和酸碱平衡的影响[J].临床麻醉学杂志,2011,27(6):587-589.
[5]李稻.功能学科实验教程[M].2版.北京:人民卫生出版社,2015:161-162.
[6]HAMZAWY M A,ABO-YOUSSEF A M,SALEM H F,et al.Antitumor activity of intratracheal inhalation of temozolomide(TM Z)loaded into gold nanoparticles and/or liposomes against urethane-induced lung cancer in BALB/c mice[J].Drug Delivery,2017,24(1):599-607.
[7]NEMOTO T,SHIBATA Y,INOUSE S,et al.Maf B silencing in macrophages does not influence the initiation and grow th of lung cancer induced by urethane[J].Excli Journal,2017(16):914-920.
[8]翟青新,何孝军,李捷.陆眠宁复合麻醉巴马小型猪超声心动图检查分析[J].中国比较医学杂志,2016,26(9):50-53.
[9]高天舒,邹飞燕,唐运莲,等.速眠新复合麻醉对外科手术实验用犬的效果观察[J].中国比较医学杂志,2006,16(6):339-340.
[10]计红,李林林,马莉,等.3种麻醉药对家兔麻醉效果的观察[J].畜牧与饲料科学,2014,35(5):16-18.
[11]余晓星,窦豆,徐海,等.酸碱平衡紊乱实验中家兔血气正常值统计[J].科技创新导报,2009(29):175-176.
[12]罗炎杰.血气分析常用指标及其临床意义[J].中国临床医生,2009,37(11):30-33.
[13]王建枝,殷莲华.病理生理学[M].8版.北京:人民卫生出版社,2013:51-59.
[14]贺绍君,李静,蔡锡英,等.麻醉药盐酸赛拉嗪对犬肝肾功能的影响[J].畜牧与兽医,2017,49(11):105-108.
[15]赵久成,公海涛.动物酸碱平衡紊乱的分类及对机体的影响[J].中国畜牧兽医文摘,2016,32(10):77.
[2]HA C W,PARK Y B,CHUNG J Y,et al.Cartilage repair using composites of human umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel in a minipig model[J].Stem Cells Translational M edicine,2015,4(9):342-347.
[3]ZHANG H,CHAI M,LIU C,et al.Endoplasmic reticulum is involved in myocardial injury in a miniature sw ine model of coronary artery stenosis exposed to acceleration—associated stress[J].Plos One,2014,10(7):541-548.
[4]陈小波,简道林.腹腔复苏对失血性休克家兔血乳酸和酸碱平衡的影响[J].临床麻醉学杂志,2011,27(6):587-589.
[5]李稻.功能学科实验教程[M].2版.北京:人民卫生出版社,2015:161-162.
[6]HAMZAWY M A,ABO-YOUSSEF A M,SALEM H F,et al.Antitumor activity of intratracheal inhalation of temozolomide(TM Z)loaded into gold nanoparticles and/or liposomes against urethane-induced lung cancer in BALB/c mice[J].Drug Delivery,2017,24(1):599-607.
[7]NEMOTO T,SHIBATA Y,INOUSE S,et al.Maf B silencing in macrophages does not influence the initiation and grow th of lung cancer induced by urethane[J].Excli Journal,2017(16):914-920.
[8]翟青新,何孝军,李捷.陆眠宁复合麻醉巴马小型猪超声心动图检查分析[J].中国比较医学杂志,2016,26(9):50-53.
[9]高天舒,邹飞燕,唐运莲,等.速眠新复合麻醉对外科手术实验用犬的效果观察[J].中国比较医学杂志,2006,16(6):339-340.
[10]计红,李林林,马莉,等.3种麻醉药对家兔麻醉效果的观察[J].畜牧与饲料科学,2014,35(5):16-18.
[11]余晓星,窦豆,徐海,等.酸碱平衡紊乱实验中家兔血气正常值统计[J].科技创新导报,2009(29):175-176.
[12]罗炎杰.血气分析常用指标及其临床意义[J].中国临床医生,2009,37(11):30-33.
[13]王建枝,殷莲华.病理生理学[M].8版.北京:人民卫生出版社,2013:51-59.
[14]贺绍君,李静,蔡锡英,等.麻醉药盐酸赛拉嗪对犬肝肾功能的影响[J].畜牧与兽医,2017,49(11):105-108.
[15]赵久成,公海涛.动物酸碱平衡紊乱的分类及对机体的影响[J].中国畜牧兽医文摘,2016,32(10):77.