摘要
针对现有表面等离子激元折射率传感器纵向探测深度小、探测范围无法覆盖整个细胞厚度的问题,提出一种大探测深度、高灵敏度的活细胞折射率实时测量方法,并利用该方法开展了药物敏感性的实验研究。基于偏振选择吸收效应,设计并搭建了全内反射条件下的石墨烯折射率传感系统,进行了不同质量分数氯化钠溶液折射率的测量,结果表明系统具有9.5×10~6 mV/RIU的灵敏度和5.5×10~(-7) RIU的分辨率;利用该系统开展了活细胞药物敏感性的实验研究,分别研究了顺铂和紫杉醇作用于Ramos细胞和Jeko-1细胞时生物演化过程中细胞折射率的实时变化规律,验证了折射率变化与其药性机理作用的一致性。
Aiming at the problem that the existing surface plasmon refractive index sensor has a small vertical detection depth and the detection range cannot cover the entire thickness of cells, we propose a real-time measurement method of the living cell refractive index for its advantages: large detection depth and high sensitivity. And this method is used to carry out the experimental research on drug susceptibility. Based on the polarization-selective absorption effect, we design and build a graphene-based refractive index sensing system under the condition of total internal reflection. The refractive index with various mass fraction of sodium chloride solution is measured. The results indicate that the sensitivity and resolution of the system is 9.5×10~6 mV/RIU and 5.5×10~(-7) RIU, respectively. The experimental study on the drug susceptibility of living cells is carried out by the system. The real-time changes of cell refractive index during the biological evolution of cisplatin and paclitaxel in Ramos cells and Jeko-1 cells are studied, and the consistency of refractive index changes with its pharmacological mechanism is verified.
引文
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