Box-Behnken响应面法优化健脾祛湿丸醇提工艺
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摘要
目的采用Box-Behnken响应面法优化健脾祛湿丸的醇提工艺。方法以盐酸小檗碱、苦参碱含量及浸膏得率的综合评分为指标,在单因素试验基础上,采用Box-Behnken响应面法考察乙醇浓度、加醇倍量和提取时间对健脾祛湿丸醇提工艺的影响。结果最佳提取工艺为:加10倍量60%乙醇,回流提取3次,每次2 h。结论本试验优选的醇提工艺稳定、可行,为工业大生产奠定了基础。
Objective To optimize the ethanol extraction process of Jianpi Qushi concentrated pills by using Box-Behnken response surface method. Methods On the basis of single factor test, taking the comprehensive scores of content of berberine hydrochloride and matrine and extract yield as indicators, the effects of ethanol concentration, alcohol addition and extraction time on alcohol extraction process were examined by Box-Behnken response surface method. Results The optimal extraction process was adding 10 times amount of 60% ethanol, reflux extracting 3 times and 2 hours each time. Conclusion The optimized ethanol extraction process is stable and predictable, which can lay foundation for industrial production of Jianpi Qushi concentrated pills.
Objective To optimize the ethanol extraction process of Jianpi Qushi concentrated pills by using Box-Behnken response surface method. Methods On the basis of single factor test, taking the comprehensive scores of content of berberine hydrochloride and matrine and extract yield as indicators, the effects of ethanol concentration, alcohol addition and extraction time on alcohol extraction process were examined by Box-Behnken response surface method. Results The optimal extraction process was adding 10 times amount of 60% ethanol, reflux extracting 3 times and 2 hours each time. Conclusion The optimized ethanol extraction process is stable and predictable, which can lay foundation for industrial production of Jianpi Qushi concentrated pills.
引文
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[10]吴吴芳妮,张芊,张春春雷.苦参在T细胞胞介导的皮肤良恶恶性疾病中的应用用进展[J].中国皮肤性病病学杂志,2016,30(8):847-849.
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[12]何何超然,李哲,李任任,等.HPLC法同时时测定山豆根中4种生物碱及其含含量[J]].中国药物应用与与监测,2018,15(2):77-81.
[13]毛毛丹,陈钶,王柯,等等.HPLC法测定苦苦参软膏中苦参碱碱、槐定碱和氧化化苦参碱碱[J].中成药,20011,33(9):1531-1534.
[14]张张江,张丽,木卡代代斯·斯依提,等.响应面法优选银银槐解毒颗粒提取取工艺[J].中国中医药信信息杂志,2018,25(1):64-68.
[2]孙森凤,张颖颖,褚褚万春.黄柏药理理作用的研究进展展[J].山东化工,2017,,46(14):99-1000.
[3]XII ANYE,MAO QQ,IPSP,,etal.Compp arisononthe e anti--inflammatorye ffectofcort exPhellodenri Chinensisand d Cortee x Phellodendri i Amurensis in12-0-tetradecann oylphorbol-13--acetaa te-induced ear redema in mic ce[J]. JEthnopp harmacol,2011,137(33):1425-1430.
[4]邢宇,刘鑫,林园,等等.小檗碱药理作用及其临床应用研究进展[J].中中国药理理学与毒理学杂志志,2017,31(6):491-502.
[5] HUAA NG L H, PAN X P P, GONG K R, et t al. Antiinflamm matory effects s of thh ree kinds of tr raditional Mongo olian medicine monomer and its s combii nation on LPS--stimulated RAW264.7 macrophagg es[J]. Eur Rev v Med PP harmacol Sci,22016,20(5):950-958.
[6]张丽丽华,陈邦恩,潘明明佳.苦参碱药理理作用研究进展[JJ].中草药,2009,40(6)):1000-1003.
[7]张明明发,沈雅琴.苦参参碱类生物碱抗病病毒的临床药理作作用研究进展[J].抗感染染药学,2018,15((2):185-191.
[8] SUNN M, CAO H, SUN N L, et al. Ant itumor activitii es of Kushen:literr ature review[J J]. Evid Based Complement Altee rnat Med,2012,2012(4):373219.
[9]陈燕燕,张丽华.苦参水提物治疗湿疹疹豚鼠皮肤炎症的的实验研究[J].现现代医院院,2015(5):22--24.
[10]吴吴芳妮,张芊,张春春雷.苦参在T细胞胞介导的皮肤良恶恶性疾病中的应用用进展[J].中国皮肤性病病学杂志,2016,30(8):847-849.
[11]国国家药典委员会.中中华人民共和国药药典:一部[M].北北京:中国医药科科技出版版社,2015:19-220,899-900.
[12]何何超然,李哲,李任任,等.HPLC法同时时测定山豆根中4种生物碱及其含含量[J]].中国药物应用与与监测,2018,15(2):77-81.
[13]毛毛丹,陈钶,王柯,等等.HPLC法测定苦苦参软膏中苦参碱碱、槐定碱和氧化化苦参碱碱[J].中成药,20011,33(9):1531-1534.
[14]张张江,张丽,木卡代代斯·斯依提,等.响应面法优选银银槐解毒颗粒提取取工艺[J].中国中医药信信息杂志,2018,25(1):64-68.