5-LO在SHR大鼠脑动脉平滑肌细胞中的表达及意义
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摘要
目的探讨5脂氧合酶(5-LO)在自发性高血压大鼠(SHR)脑动脉平滑肌细胞中的表达及意义。方法选取16周龄雄性SHR和WKY大鼠,各15只,WKY大鼠作为正常对照。采用免疫荧光法检测5-LO在脑动脉血管中的表达,透射电镜观察动脉内膜表现,ELISA检测血清及动脉5-LO表达。结果 WKY大鼠动脉内膜光滑,平滑肌细胞排列整齐无增生,SHR大鼠内皮细胞肿胀,平滑肌细胞肥大变形排列紊乱;SHR大鼠脑动脉平滑肌细胞5-LO荧光强度为(110.93±5.92),明显强于WKY大鼠(P<0.05);SHR大鼠血清和脑动脉5-LO水平分别为(2671.04±30.18)ng/l和(1950.04±30.08)ng/l,明显高于WKY大鼠,差异比较有统计学意义(P<0.05)。结论 5-LO在SHR大鼠脑动脉平滑肌细胞和血液中表达异常增高,可能在高血压脑血管损害过程中起作用。
Objective To investigate the expression and significance of 5-lipoxygenase(5-LO)in the cerebral artery smooth cells of spontaneously hypertensive rats(SHR).Methods 16 weeks old male 15 SHR rats and 15 WKY rats were selected in the present study.The expression of 5-LO in cerebral artery was detected by immunofluorescence.Transmission electron microscopy was used to observe the performance of arterial intimal.ELISA was used to detect the expression of 5-LO in serum and artery.Results The artery intimal of WKY rat was smooth.The smooth muscle cells of WKY rat orderly arranged without hyperplasia.The endothelial cell of SHR rat was swelled.The smooth muscle cell of SHR was hypertrophy disorder.The fluorescence intensity of 5-LO in rat cerebral artery smooth muscle cells of SHR rat(110.93±5.92)was significantly stronger than that of WKY rats(P <0.05).The levels of 5-LO in serum and cerebral artery of SHR rats were significantly higher than that of WKY rats(P <0.05).Conclusion The expression of 5-LO in rat cerebral artery smooth muscle cells and blood are abnormal increased,which may play a role in the course of hypertensive cerebral vascular damage.
Objective To investigate the expression and significance of 5-lipoxygenase(5-LO)in the cerebral artery smooth cells of spontaneously hypertensive rats(SHR).Methods 16 weeks old male 15 SHR rats and 15 WKY rats were selected in the present study.The expression of 5-LO in cerebral artery was detected by immunofluorescence.Transmission electron microscopy was used to observe the performance of arterial intimal.ELISA was used to detect the expression of 5-LO in serum and artery.Results The artery intimal of WKY rat was smooth.The smooth muscle cells of WKY rat orderly arranged without hyperplasia.The endothelial cell of SHR rat was swelled.The smooth muscle cell of SHR was hypertrophy disorder.The fluorescence intensity of 5-LO in rat cerebral artery smooth muscle cells of SHR rat(110.93±5.92)was significantly stronger than that of WKY rats(P <0.05).The levels of 5-LO in serum and cerebral artery of SHR rats were significantly higher than that of WKY rats(P <0.05).Conclusion The expression of 5-LO in rat cerebral artery smooth muscle cells and blood are abnormal increased,which may play a role in the course of hypertensive cerebral vascular damage.
引文
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[2]Tsai CM,Kuo HC,Hsu CN,et al.Metformin reduces asymmetric dimethylarginine and prevents hypertension in spontaneously hypertensive rats[J].Translational Research,2014,164(6):452-459.
[3]李立新,方宁远.持续静脉泵入乌拉地尔与泵入硝普钠治疗高血压急症的效果比较[J].西部医学,2015,27(3):356-357.
[4]何思陆,李天资.FXYD5在高血压病动脉粥样硬化中调控机制的研究进展[J].中西医结合心脑血管病杂志,2015,18(15):1730-1732.
[5]俞书红,蓝文雅,李壮丽,等.黄芩苷对球囊拉伤后颈动脉粥样硬化的影响及其作用机制[J].中华老年心脑血管病杂志,2016,18(4):412-415.
[6]戴志强,张旋,冯阳,等.高盐诱导自发性高血压大鼠脑出血及脑微血管构筑改变[J].第三军医大学学报,2016,38(21):2291-2296.
[7]王喆.高血压合并脑血管病的降压治疗[J].中国医刊,2014,64(6):6-8.
[8]欧军,赵红,谢红,等.七氟醚预处理对脊髓缺血再灌注大鼠5脂氧合酶表达的影响[J].中国现代医学杂志,2015,25(25):12-15.
[9]徐小桥,金雅,黄磊瑛,等.抗白三烯治疗对哮喘患儿半胱胺酰白三烯及5脂氧合酶激活蛋白基因水平的影响[J].中国综合临床,2015,31(6):517-520.
[10]王璐璐,王雁,隋爱华,等.5-脂氧合酶激活蛋白基因多态性增加阿司匹林抵抗易感性简[J].中华老年心脑血管病杂志,2016,18(10):1053-1056.
[11]董晓,柯尊平,王俊峰,等.分段扩增法克隆大鼠5-脂肪氧合酶cDNA并鉴定其序列[J].中华老年心脑血管病杂志,2016,18(3):300-304.
[12]Jensen IJ,Eysturskare J,Madetoja M,et al.The potential of cod hydrolyzate to inhibit blood pressure in spontaneously hypertensive rats.[J].Nutrition Research,2014,34(2):168-173.
[13]彭清,卢建刚,王小洁,等.高血压病患者血清chemerin与胰岛素抵抗及炎症的关系[J].西部医学,2014,26(1):37-39.
[14]Liu DZ,Liang YC,Lin SY,et al.Antihypertensive Activities of a Solid-State Culture of(Chang-Chih)in Spontaneously Hypertensive Rats[J].Bioscience Biotechnology&Biochemistry,2014,71(1):23-30.
[15]王维刚,郭莉莉.不同脉压对老年高血压患者心脑血管事件的影响分析[J].心脑血管病防治,2016,16(5):351-352.
[16]简孝洪.中西医结合治疗高血压[J].中国地方病防治杂志,2014,23(s1):92-92.
[17]陈浥尘,蒋柳萍,车在前,等.不同遗传性高血压大鼠的主动脉超微结构与血管功能损伤[J].中华高血压杂志,2014,22(8):728-736.
[18]张宇,傅义程,魏国良,等.线粒体功能在自发性高血压大鼠动脉平滑肌细胞中的改变与机制探究[J].中国煤炭工业医学杂志,2015,18(11):1892-1897.
[19]郭丽,赵连友,刘静,等.高血压大鼠血管平滑肌细胞内质网应激时葡萄糖调节蛋白94与C/EBP同源蛋白的表达及氨氯地平对其的干预作用[J].临床内科杂志,2014,31(7):492-495.
[20]黄茂葵,汪根树,张相强,等.5-脂氧合酶在缺血脑组织和脑脊髓损伤及阿尔茨海默病中的作用[J].中国脑血管病杂志,2016,13(4):213-215.
[21]徐小桥,金雅,黄磊瑛,等.抗白三烯治疗对哮喘患儿半胱胺酰白三烯及5脂氧合酶激活蛋白基因水平的影响[J].中国综合临床,2015,31(6):156-157.
[22]黄凯,蒋世云,陈柳军.5-脂氧合酶及其抑制剂[J].中国生物化学与分子生物学报,2014,30(12):1190-1196.
[23]董晓,王俊峰,李军,等.尾加压素Ⅱ促进大鼠主动脉球囊拉伤血管新生内膜形成和5-脂肪氧合酶的表达[J].中华老年心脑血管病杂志,2015,17(2):188-192.
[24]涂献坤,杨卫忠,石松生,等.黄芩苷通过抑制5-脂氧合酶活性减轻大鼠脑缺血炎症反应和脂质过氧化反应[J].解剖学杂志,2014,37(1):51-53.
[25]唐石树,胡建安.5-脂氧合酶在人体组织中的表达及其与疾病的关系[J].中南大学学报(医学版),2015,40(4):438-445.