Glut1在腹膜恶性间皮瘤诊断和鉴别诊断中的作用
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摘要
目的通过检测葡萄糖转运蛋白1(Glut1)在腹膜恶性间皮瘤、卵巢癌和反应性间皮细胞增生中的表达,探讨Glut1在腹膜恶性间皮瘤(PMM)病理诊断和鉴别诊断中的作用。方法采用免疫组织化学方法检测Glut1在各30例PMM、卵巢癌及反应性间皮细胞增生中的表达情况,分析Glut1在PMM病理诊断和鉴别诊断中的作用。结果 Glut1在PMM、卵巢癌、反应性间皮细胞增生中的阳性表达率分别为66.7%,93.3%和0。Glut1在PMM在与卵巢癌中的表达率差异有统计学意义(P<0.01)。在PMM与反应性间皮细胞增生中表达率差异亦有统计学意义(P<0.001)。结论 PMM与反应性间皮细胞增生鉴别诊断时,Glut1免疫标记有确切的鉴别诊断价值。在与卵巢癌进行鉴别诊断时,虽然两者阳性表达率均较高,但阳性表达强度明显不同,卵巢癌为强阳性表达。此时一同运用其他PMM阳性及阴性免疫标记物才更有诊断价值。
Objective To investigate the expression of glucose transporter 1(Glut1) in peritoneal malignant mesothelioma,ovarian cancer and reactive mesothelial cell hyperplasia, and to explore the role of Glut1 in the pathological diagnosis and differential diagnosis of peritoneal malignant mesothelioma(PMM). Methods Immunohistochemistry was used to detect the expression of Glut1 in 30 cases of PMM, ovarian cancer and reactive mesothelial cell proliferation. The role of Glut1 in pathological diagnosis and differential diagnosis of PMM was analyzed. Results The positive rates of Glut1 in PMM, ovarian cancer and reactive mesothelial cell proliferation were 66.7%, 93.3% and 0, respectively. There was a significant difference in the expression of Glut1 between PMM and ovarian cancer(P<0.01). The difference in expression rate between reactive mesothelial cells and proliferation was also statistically significant(P<0.001). Conclusion The differential diagnosis of Glut1 immunolabeling in the differential diagnosis of PMM and reactive mesothelial hyperplasia is of value. In the differential diagnosis with ovarian cancer, although the positive expression rate of both is high, the positive expression intensity is obviously different, and the ovarian cancer shows strongly positive expression. It is of more diagnostic value to use other PMM positive and negative immunological markers together.
Objective To investigate the expression of glucose transporter 1(Glut1) in peritoneal malignant mesothelioma,ovarian cancer and reactive mesothelial cell hyperplasia, and to explore the role of Glut1 in the pathological diagnosis and differential diagnosis of peritoneal malignant mesothelioma(PMM). Methods Immunohistochemistry was used to detect the expression of Glut1 in 30 cases of PMM, ovarian cancer and reactive mesothelial cell proliferation. The role of Glut1 in pathological diagnosis and differential diagnosis of PMM was analyzed. Results The positive rates of Glut1 in PMM, ovarian cancer and reactive mesothelial cell proliferation were 66.7%, 93.3% and 0, respectively. There was a significant difference in the expression of Glut1 between PMM and ovarian cancer(P<0.01). The difference in expression rate between reactive mesothelial cells and proliferation was also statistically significant(P<0.001). Conclusion The differential diagnosis of Glut1 immunolabeling in the differential diagnosis of PMM and reactive mesothelial hyperplasia is of value. In the differential diagnosis with ovarian cancer, although the positive expression rate of both is high, the positive expression intensity is obviously different, and the ovarian cancer shows strongly positive expression. It is of more diagnostic value to use other PMM positive and negative immunological markers together.
引文
[1]邢炜,付静,刘繁荣,等.腹膜恶性间皮瘤临床病理观察[J].现代肿瘤医学,2016,24(11):1752-1756.
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[4]蔡昱,邵淑丽,王全红,等.GLUT-1和DNA-PKcs在卵巢浆液性肿瘤组织中的表达及意义[J].癌症,2007,26(11):1188-1193.
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[7]董春花,李福琴,原丹丹,等.GLUT1、VEGF在上皮性卵巢癌中的表达和意义[J].哈尔滨医科大学学报,2006,40(5):379-382
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[9]张丽芬,许玉珍,赵金红.缺氧诱导因子-1a、葡萄糖转运蛋白1在卵巢上皮性癌组织中的表达及其意义[J].中国综合临床,2007,23(9):842-845.
[10]赵书君,刘俊英,任芬若,等.葡萄糖转运蛋白1在卵巢上皮性癌组织中的表达及其与碱性成纤维生长因子、增殖细胞核抗原表达的关系,2005,40(4):264-266.
[11]申彦,张新莹,师宜荃,等.Glut-1、HIF-1a及P53在卵巢癌中的表达及意义[J].天津医科大学学报,2013,19(5):383-386.
[12]杨春林.缺氧诱导因子-1a和葡萄糖转运蛋白1在卵巢上皮性癌中的表达及其意义[J].中国医师进修杂志,2014,31(6):57-59.
[13]杨通印,郝朗松,郭德正.缺氧诱导因子1α、葡萄糖转运蛋白1和乳酸脱氢酶5在结直肠癌的表达及其意义[J].中华病理学杂志,2015,44(11):767-771.
[14]张伟,王科嘉,于文娟.S1OOAl、GLUT-1及Caveolin-1在嗜酸细胞性肾肿瘤中的表达及其鉴别诊断意义[J].中华病理学杂志,2017,46(2):93-97.
[15]郭慧玲,刘睿,王红,GLUT1和PKM2在二甲双胍抑制人乳头状甲状腺癌细胞增殖中的作用[J].现代肿瘤医学,2015,23(7):900-904.
[16]李卫萍,张磊,申艳方,等.葡萄糖转运蛋白1在阿尔茨海默病中的作用研究进展[J].中国医药导报,2017,14(14):36-39.
[17]刘标,周晓军.恶性间皮瘤的病理诊断[J].诊断病理学杂志,2014,21(1):1-3.
[18] Ucer O,Dagli AF,Kilicarslan A.Value of Glut-1 and Kocmarkers in the differential diagnosis of reactive mesothelial hyperplasia,malignant mesothelioma and pulmonary adenocarcinoma[J].Turk Patoloji Derg,2013,29(2):94-100.
[19] Husain AN,Mirza MK,Gibbs A.How useful is GLUT-1in differantiating mesothelial hyperplasia and fibrosing pleuritis from epithelioid and sarcomatoid mesotheliomas?An international collaborative study[J].Lung Cancer,2014,83(3):324-328.
[20] Kato Y,Tsute K,Seki K.Immunohistochemical detection of GLUT-1 can discriminate between reactive mesothelium and malignant mesothelioma[J].Mod Pathol,2007,20(2):215-220.
[21]刘影,陈晓玲,丁宁宁,等.荧光原位杂交与免疫组织化学检测P16在恶性间皮瘤中的表达[J].中华病理学杂志,2015,44(4):262-265.
[22]赵雨,冯瑞娥,曾瑄,等. 64例胸膜恶性间皮瘤的临床病理分析[J].中国肺部疾病杂志,2016,9(6):590-595.
[2]楼建林,高志斌,蒋兆强,等.中日专家对恶性间皮瘤病理诊断的对比研究[J].中华劳动卫生职业病杂志,2015,33(1):33-36.
[3]陈日萍,楼建林,张幸.恶性间皮瘤病理诊断指南-2012年国际间皮瘤学会共同声明更新[J].中华劳动卫生职业病杂志,2015,33(11):860-863.
[4]蔡昱,邵淑丽,王全红,等.GLUT-1和DNA-PKcs在卵巢浆液性肿瘤组织中的表达及意义[J].癌症,2007,26(11):1188-1193.
[5]俞晶,黄云超,张丽娟,等.HIF-1a和GLUT-1在卵巢癌中的表达及其临床意义[J].临床肿瘤学杂志,2009,14(3):207-209.
[6]余英豪,刘伟.恶性间皮瘤的免疫组化诊断[J].诊断病理学杂志,2014,21(5):257-259.
[7]董春花,李福琴,原丹丹,等.GLUT1、VEGF在上皮性卵巢癌中的表达和意义[J].哈尔滨医科大学学报,2006,40(5):379-382
[8]邹积艳,潘颖,庞晓丽,等.卵巢癌组织中GLUT-1及MMP-2的的表达和临床意义[J].中国妇幼保健,2006,21(13):1830-1832.
[9]张丽芬,许玉珍,赵金红.缺氧诱导因子-1a、葡萄糖转运蛋白1在卵巢上皮性癌组织中的表达及其意义[J].中国综合临床,2007,23(9):842-845.
[10]赵书君,刘俊英,任芬若,等.葡萄糖转运蛋白1在卵巢上皮性癌组织中的表达及其与碱性成纤维生长因子、增殖细胞核抗原表达的关系,2005,40(4):264-266.
[11]申彦,张新莹,师宜荃,等.Glut-1、HIF-1a及P53在卵巢癌中的表达及意义[J].天津医科大学学报,2013,19(5):383-386.
[12]杨春林.缺氧诱导因子-1a和葡萄糖转运蛋白1在卵巢上皮性癌中的表达及其意义[J].中国医师进修杂志,2014,31(6):57-59.
[13]杨通印,郝朗松,郭德正.缺氧诱导因子1α、葡萄糖转运蛋白1和乳酸脱氢酶5在结直肠癌的表达及其意义[J].中华病理学杂志,2015,44(11):767-771.
[14]张伟,王科嘉,于文娟.S1OOAl、GLUT-1及Caveolin-1在嗜酸细胞性肾肿瘤中的表达及其鉴别诊断意义[J].中华病理学杂志,2017,46(2):93-97.
[15]郭慧玲,刘睿,王红,GLUT1和PKM2在二甲双胍抑制人乳头状甲状腺癌细胞增殖中的作用[J].现代肿瘤医学,2015,23(7):900-904.
[16]李卫萍,张磊,申艳方,等.葡萄糖转运蛋白1在阿尔茨海默病中的作用研究进展[J].中国医药导报,2017,14(14):36-39.
[17]刘标,周晓军.恶性间皮瘤的病理诊断[J].诊断病理学杂志,2014,21(1):1-3.
[18] Ucer O,Dagli AF,Kilicarslan A.Value of Glut-1 and Kocmarkers in the differential diagnosis of reactive mesothelial hyperplasia,malignant mesothelioma and pulmonary adenocarcinoma[J].Turk Patoloji Derg,2013,29(2):94-100.
[19] Husain AN,Mirza MK,Gibbs A.How useful is GLUT-1in differantiating mesothelial hyperplasia and fibrosing pleuritis from epithelioid and sarcomatoid mesotheliomas?An international collaborative study[J].Lung Cancer,2014,83(3):324-328.
[20] Kato Y,Tsute K,Seki K.Immunohistochemical detection of GLUT-1 can discriminate between reactive mesothelium and malignant mesothelioma[J].Mod Pathol,2007,20(2):215-220.
[21]刘影,陈晓玲,丁宁宁,等.荧光原位杂交与免疫组织化学检测P16在恶性间皮瘤中的表达[J].中华病理学杂志,2015,44(4):262-265.
[22]赵雨,冯瑞娥,曾瑄,等. 64例胸膜恶性间皮瘤的临床病理分析[J].中国肺部疾病杂志,2016,9(6):590-595.