纳米金修饰的碳纤维超微电极在高浓度抗坏血酸体系中选择性测定多巴胺
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摘要
将柠檬酸三钠与硼氢化钠还原氯金酸制备纳米金颗粒,采用一步恒电位沉积的方法在碳纤维超微电极上沉积纳米金颗粒,并对电极进行电化学表征。分别对100μmol/L DA、1mmol/L AA在该电极上修饰前后的电化学行为进行了研究,结果表明在浓度为1 mmol/L抗坏血酸共存下,DA的浓度(0. 1～10μmol/L)与氧化峰电流成正比,线性回归方程为Ip(μA)=200 c(μmol/L)+2×10~(-4),相关系数R~2=0. 9979,线性范围0. 1～10μmol/L,检出限为1. 28×10~(-2)μmol/L (S/N=3)。方法可用于较高浓度抗坏血酸共存下对多巴胺的选择性测定。
The nanogold-modified carbon fiber electrode( CFE) was prepared with electrodeposition,the electrochemical behavior of nanogold/CFE was characterized by potassium ferricyanide,dopamine( DA),norepinephrine( NE),and ascorbic acid( AA) using cyclic voltammetry( CV) and differential pulse voltammetry( DPV). The experimental results showed that in the 20 mmol/L TrisHCl( pH7. 0) buffer solution,the modified electrode can be selectively used to detect DA without interference from high concentration ascorbic acid( AA). Under the presence of 1. 0 mmol/L AA,the different pulse voltammetric response of the electrode showed a linear relationship with DA concentration in the range of 0. 1 ～ 10μmol/L. The regressive equation was Ip( μA) = 200 c( μmol/L) + 2 × 10~(-4)( r~2= 0. 9979),and the detection limit was 1. 28 × 10~(-2)μmol/L( S/N = 3).This method can be applied to the determination of dopamine selective in the higher concentrations of ascorbic acid.
The nanogold-modified carbon fiber electrode( CFE) was prepared with electrodeposition,the electrochemical behavior of nanogold/CFE was characterized by potassium ferricyanide,dopamine( DA),norepinephrine( NE),and ascorbic acid( AA) using cyclic voltammetry( CV) and differential pulse voltammetry( DPV). The experimental results showed that in the 20 mmol/L TrisHCl( pH7. 0) buffer solution,the modified electrode can be selectively used to detect DA without interference from high concentration ascorbic acid( AA). Under the presence of 1. 0 mmol/L AA,the different pulse voltammetric response of the electrode showed a linear relationship with DA concentration in the range of 0. 1 ～ 10μmol/L. The regressive equation was Ip( μA) = 200 c( μmol/L) + 2 × 10~(-4)( r~2= 0. 9979),and the detection limit was 1. 28 × 10~(-2)μmol/L( S/N = 3).This method can be applied to the determination of dopamine selective in the higher concentrations of ascorbic acid.
引文
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