规模化猪场副猪嗜血杆菌、多杀性巴氏杆菌和化脓隐秘杆菌控制性研究
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摘要
河南某规模化猪场断奶仔猪突发疫病,病猪以高烧、喘气、呼吸困难为主要临床症状,以肺炎、肺脏有化脓灶,淋巴结肿大为主要病理变化,通过实验室细菌分离鉴定、生化试验、致病性试验、PCR鉴定等检验,确诊该疫病是由副猪嗜血杆菌、多杀性巴氏杆菌和化脓隐秘杆菌混合感染所致。药敏试验显示,这3种细菌对磺胺类、大环内酯类和四环素类抗生素都有一定程度的耐药性,对喹诺酮类和β-内酰胺类抗生素高度敏感。药敏试验结果对指导猪场控制本次疫情临床用药有明显的重要意义。
The epidemic disease of weaned pigs in a large-scale pig farm in Henan province was characterized by high fever,gasping, and dyspnea as the main clinical symptoms. The main pathological changes were pneumonia, lung pyoderma and lymphadenopathy. Through laboratory tests for bacterial isolation, biochemical tests, pathogenicity tests, and PCR assays, the confirmed epidemic was caused by mixed infections with Haemophilus parasuis, Pasteurella multocida, and Arcanobacterium pyogenes. Drug susceptibility testing showed that these three bacteria had a certain degree of resistance to sulfonamides, macrolides, and tetracycline antibiotics, and were highly sensitive to quinolones and β-lactam antibiotics. Drug susceptibility test results which are used to guide pigs farm to control the clinical use of the epidemic has significant significance.
The epidemic disease of weaned pigs in a large-scale pig farm in Henan province was characterized by high fever,gasping, and dyspnea as the main clinical symptoms. The main pathological changes were pneumonia, lung pyoderma and lymphadenopathy. Through laboratory tests for bacterial isolation, biochemical tests, pathogenicity tests, and PCR assays, the confirmed epidemic was caused by mixed infections with Haemophilus parasuis, Pasteurella multocida, and Arcanobacterium pyogenes. Drug susceptibility testing showed that these three bacteria had a certain degree of resistance to sulfonamides, macrolides, and tetracycline antibiotics, and were highly sensitive to quinolones and β-lactam antibiotics. Drug susceptibility test results which are used to guide pigs farm to control the clinical use of the epidemic has significant significance.
引文
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[3]张群.猪细菌性呼吸道病的发生及控制措施[J].安徽农学通报,2013,19(8):122-123.
[4]徐引弟,张青娴,李建林,等.猪伪狂犬病毒、副猪嗜血杆菌和猪链球菌混合感染的病原分离与鉴定[J].河南农业科学,2015,44(9):100-104.
[5]徐引弟,王治方,朱文豪,等.猪多杀性巴氏杆菌的分离鉴定和药敏试验[J].中国兽药杂志,2011,45(5):5-7.
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[7]王乐.副猪嗜血杆菌的分离鉴定及生物特性研究[D].洛阳:河南科技大学,2014.
[8]陆承平.兽医微生物学[M].4版.北京:中国农业出版社,2008:112-114.
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[10]鲁杏华,何世成,谈志祥,等.猪化脓隐秘杆菌的分离与鉴定[J].中国畜牧兽医,2009,20(5):149-151.
[11]费育超,倪宏波.副猪嗜血杆菌与圆环病毒混合感染的诊断[J].现代畜牧兽医,2017(1):38-41.