猪伪狂犬病排毒情况与gE、gB抗体之间的关系研究
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摘要
采集猪伪狂犬病野毒阳性场和阴险场各阶段猪群的血清、乳汁、唾液等样品,用ELISA检测血清gE和gB抗体水平,结果显示,阳性场gE总阳性率为56.7%,其中只有哺乳母猪和1周龄仔猪群的gE呈阴性,6~24周龄猪群gE阳性率从52.9%上升至100%,经产和后备母猪群阳性率高达90%~100%;gB总体阳性率为88.8%,母猪群和公猪阳性率达100%,从1周龄仔猪阳性率100%逐渐降至10周龄育肥猪的40%,之后又快速升高到100%。阴性场gE阴性,gB总体阳性率为38.9%,2~12周龄猪群的gB逐渐转阴,14~20周龄猪群阳性率在20%~75%之间,母猪和公猪群gB阳性率达100%。用RT-PCR检测样品中的核酸,阳性场中脐带血的阳性率最高、达到80%,之后是初乳、为33.3%,其他拭子样品带毒率较低、0.55%~6.21%不等,阴性场样品均未扩增出gE基因片段。研究结果说明,阳性场与阴性场相比,PRV带毒、排毒途径多样,以母猪垂直传播为主,PRV排毒不仅与gE抗体存在相关性,还对母源抗体和免疫抗体产生干扰,表现在母源抗体和免疫消退期gE和gB抗体异常升高。
Different samples including serum,milk, nasal swabs and so on,in different stages were collected from PRV-postitive and negative farm in present study,the level of gE and gB antibody were detected by ELISA,The result showed that the total positive rate of gE was 56.7%,only lactating sows and 1 week piglets were negative,the level of antibody of 6 to 24-week pig increased gradually from 52.9% to 100%,while the effection rate was up to 90%-100% in sows including(perinatal and backup period sow) .The total level of gB antibody was 88.8%,the positive rate in sow and boar were up to 90%-100%,the positive rate of 1 week-old piglets was 100% and then gradually reduced to 40%,after that the positive rate increased quickly after 10 week. The negative farm showed that the gE was negative,and the positive rate of gB was 38.9%,especially for boar sow,their rate was up to 100%,the gB antibody turned to be negative from 2 week to 12 week,the gB positive rate of 14 week-old to 20 week-old fishing pigs was 20%-75%. RT-PCR was performed to detect PRV nucleotide,the result of positive farm showed a widely effection in the deferent herd,except semen,especially the PRV-positive rate in cord blood was highest,up to 80%,colostrum,33.3%,the positive rate of swabs were low,range of 0.55 to 6.21%. these results account for the various ways of shedding and release of PRV, vertical transmission is predominate method of transmission in our study,compared to negative,the PRV release not only was relationship with gE antibody,but also disturbed maternal antibodies and immune antibodies,the gE and gB antibodies were significantly abnormally increased when maternal and immune antibodies were fading away.
Different samples including serum,milk, nasal swabs and so on,in different stages were collected from PRV-postitive and negative farm in present study,the level of gE and gB antibody were detected by ELISA,The result showed that the total positive rate of gE was 56.7%,only lactating sows and 1 week piglets were negative,the level of antibody of 6 to 24-week pig increased gradually from 52.9% to 100%,while the effection rate was up to 90%-100% in sows including(perinatal and backup period sow) .The total level of gB antibody was 88.8%,the positive rate in sow and boar were up to 90%-100%,the positive rate of 1 week-old piglets was 100% and then gradually reduced to 40%,after that the positive rate increased quickly after 10 week. The negative farm showed that the gE was negative,and the positive rate of gB was 38.9%,especially for boar sow,their rate was up to 100%,the gB antibody turned to be negative from 2 week to 12 week,the gB positive rate of 14 week-old to 20 week-old fishing pigs was 20%-75%. RT-PCR was performed to detect PRV nucleotide,the result of positive farm showed a widely effection in the deferent herd,except semen,especially the PRV-positive rate in cord blood was highest,up to 80%,colostrum,33.3%,the positive rate of swabs were low,range of 0.55 to 6.21%. these results account for the various ways of shedding and release of PRV, vertical transmission is predominate method of transmission in our study,compared to negative,the PRV release not only was relationship with gE antibody,but also disturbed maternal antibodies and immune antibodies,the gE and gB antibodies were significantly abnormally increased when maternal and immune antibodies were fading away.
引文
[1]谢海燕,温清萍,王健青.广东省东莞市2010—2014年猪伪狂犬病野毒抗体监测与分析[J].流行病学,2016(33):7-9.
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[3]斯特劳,阿莱尔,蒙加林,等.猪病学[M].北京:中国农业大学出版社,2000.
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[5]龙晓婷.伪狂犬病毒在潜伏感染组织内的分布[J].畜牧兽医学报,2008,39(5):645-651.
[6]司兴奎,尹庆亮,张济培,等.华南某种鸡场禽白血病病毒感染对4种免疫抗体水平的影响[J].中国家禽,2009(31):12-15.
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[8]王军.导致猪场免疫失败的“十大”关键点[J].今日养猪业,2017(1):94-97.
[9]郝飞,汤德元,曾智勇,等.猪伪狂犬病基因工程疫苗的研究进展[J].猪业科学.2013(3):92-94.
[10]刘明亚,陈闻,李玉峰.猪瘟和伪狂犬抗体水平对疫苗免疫的影响[ J].畜牧与兽医,2010,42(2):68-70.
[11]朱玲,郭万柱,徐志文.猪伪狂犬病基因缺失活疫苗(SA215)免疫母猪后仔猪母源抗体消长规律及首免日龄[J].中国兽医学报,2004,24(4):320-322.
[12]邱美珍,傅胜才,杜丽飞,等.湖南省部分规模猪场伪狂犬病野毒血清流行病学系统监测与净化分析[J].养猪,2012(3):89-92.
[13]蒋智勇,蔡汝健,李艳,等.母猪乳汁中猪伪狂犬病病毒的分离与鉴定//中国畜牧兽医学会动物传染病学分会第十六次学术研讨会[C].北京:中国畜牧兽医学会,2015.
[14]邱美珍,李海辉,杨俊,等.猪伪狂犬病阳性场g E与g B抗体监测及风险评估[J].养猪,2014(6):124-126.
[15]李磊,史景春,张静.猪伪狂犬病发生原因分析及净化[J].畜牧兽医科技信息,2014(12):78-79.
[2]Yu X, Zhou Z, Hu D,et al.Pathogenic pseudorabies virus,China,2012[J].Emerging Infectious Diseases,2014,20(1):102-104.
[3]斯特劳,阿莱尔,蒙加林,等.猪病学[M].北京:中国农业大学出版社,2000.
[4]任裕其,卢洪芬,梁红梅,等.广东省猪伪狂犬病的现状及其发展趋势[J].中国兽医杂志.1994(3):25-26.
[5]龙晓婷.伪狂犬病毒在潜伏感染组织内的分布[J].畜牧兽医学报,2008,39(5):645-651.
[6]司兴奎,尹庆亮,张济培,等.华南某种鸡场禽白血病病毒感染对4种免疫抗体水平的影响[J].中国家禽,2009(31):12-15.
[7]殷震,刘景华.动物病毒学[M].北京:科学出版社,1998:1137-1182.
[8]王军.导致猪场免疫失败的“十大”关键点[J].今日养猪业,2017(1):94-97.
[9]郝飞,汤德元,曾智勇,等.猪伪狂犬病基因工程疫苗的研究进展[J].猪业科学.2013(3):92-94.
[10]刘明亚,陈闻,李玉峰.猪瘟和伪狂犬抗体水平对疫苗免疫的影响[ J].畜牧与兽医,2010,42(2):68-70.
[11]朱玲,郭万柱,徐志文.猪伪狂犬病基因缺失活疫苗(SA215)免疫母猪后仔猪母源抗体消长规律及首免日龄[J].中国兽医学报,2004,24(4):320-322.
[12]邱美珍,傅胜才,杜丽飞,等.湖南省部分规模猪场伪狂犬病野毒血清流行病学系统监测与净化分析[J].养猪,2012(3):89-92.
[13]蒋智勇,蔡汝健,李艳,等.母猪乳汁中猪伪狂犬病病毒的分离与鉴定//中国畜牧兽医学会动物传染病学分会第十六次学术研讨会[C].北京:中国畜牧兽医学会,2015.
[14]邱美珍,李海辉,杨俊,等.猪伪狂犬病阳性场g E与g B抗体监测及风险评估[J].养猪,2014(6):124-126.
[15]李磊,史景春,张静.猪伪狂犬病发生原因分析及净化[J].畜牧兽医科技信息,2014(12):78-79.