苯丙氨酸取代的丝氨酸八聚体离子的红外光解离光谱及其在手性分析中的应用
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摘要
通过电喷雾电离的方法,结合质谱-红外解离光谱技术对苯丙氨酸取代的丝氨酸八聚体团簇离子的红外解离光谱手性效应进行了研究,获得了相应的团簇离子在2 700~3 600 cm~(-1)的红外解离光谱。实验结果显示,苯丙氨酸取代的丝氨酸八聚体团簇离子均存在明显的同手性优势。通过比较相应红外解离光谱图的差异或在特定波长处的红外解离质谱均可实现苯丙氨酸单元的手性区分。
A method of infrared photodissociation( IRPD) spectroscopy was developed for the determination of phenylalanine substituted L-serine octamer ions. The phenylalanine substituted L-serine octamer ions were generated by electrospray ionization( ESI) and studied by mass spectrometry( MS) coupled with infrared photodissociation( IRPD) spectroscopy. Chiral preference was found existing in such cluster ions. IRPD experiments showed that the ions of [L-Ser_7+ L/D-Phe_1] H+and [L-Ser_6+L/D-Phe_2] H+were observed in the range of 2 700-3 600 cm~(-1). Chiral differentiation was fulfilled by comparing their IRPD spectra or IRPD mass spectra obtained at some special wavenumbers.
A method of infrared photodissociation( IRPD) spectroscopy was developed for the determination of phenylalanine substituted L-serine octamer ions. The phenylalanine substituted L-serine octamer ions were generated by electrospray ionization( ESI) and studied by mass spectrometry( MS) coupled with infrared photodissociation( IRPD) spectroscopy. Chiral preference was found existing in such cluster ions. IRPD experiments showed that the ions of [L-Ser_7+ L/D-Phe_1] H+and [L-Ser_6+L/D-Phe_2] H+were observed in the range of 2 700-3 600 cm~(-1). Chiral differentiation was fulfilled by comparing their IRPD spectra or IRPD mass spectra obtained at some special wavenumbers.
引文
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[6] Kumari S,Prabhakar S,Vairamani M,Devi C L,Chaitanya G K,Bhanuprakash K. J. Am. Soc. Mass Spectrom.,2007,18:1516-1524.
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[8] Kong X L. Rapid Commun. Mass Spectrom.,2012,26:870-873.
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[10] Cong X,Czerwieniec G,McJimpsey E,Ahn S,Troy F A,Lebrilla C. B. J. Am. Soc. Mass Spectrom.,2006,17:442-452.
[11] Filippi A,Gasparrini F,Pierini M,Speranza M,Villani C. J. Am. Chem. Soc.,2005,127:11912-11913.
[12] Xu N S,Yang H M,Cui M,Song F R,Liu Z Q,Liu S Y. Chem. J. Chin. Univ.(徐牛生,杨洪梅,崔勐,宋凤瑞,刘志强,刘淑莹.高等学校化学学报),2012,11:2430-2434.
[13] Chu Y Q,Dai Z Y,Jiang G Y,Zheng S B,Chen X,Ding C F. Chem. J. Chin. Univ.(储艳秋,戴兆云,蒋公羽,郑松柏,陈玺,丁传凡.高等学校化学学报),2009,5:869-875.
[14] Liao G H,Yang Y J,Kong X L. Phys. Chem. Chem. Phys.,2014,16:1554-1558.
[15] Ren J,Wang Y Y,Feng R X,Kong X L. Chin. Chem. Lett.,2017,28:537-540.
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[18] Takats Z,Nanita S C,Cooks R G. Angew. Chem. Int. Ed.,2003,42:3521-3523.
[19] Ren J,Bian S,Wang Y Y,Kong X L. Prog. Chem.(任娟,边申,王奕允,孔祥蕾.化学进展),2018,30:383-397.
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