响应面法优化土鳖虫水溶性蛋白质的提取条件
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摘要
目的确定土鳖虫水溶性蛋白质提取最佳工艺条件。方法在单因素实验基础上,以土鳖虫蛋白质提取率(Y)为响应值,应用响应面法对土鳖虫蛋白质提取工艺进行优化。结果依据统计模型发现液料比、提取时间、提取液p H 3个要素均对土鳖虫蛋白质提取率影响明显。由响应面三维图及等值图分析,得到土鳖虫蛋白质提取率较高的最佳方案为液料比40:1,提取时间20 min,提取液p H 9。结论验证实验所得蛋白质提取率为11.06%,与模型预测值十分接近,运用响应面法优化土鳖虫蛋白质提取条件切实可行。
Objective To explore the optimal extraction conditions of water-soluble proteins from Eupolyphaga steleophaga. Methods Based on the results of single-factor tests, the extraction process of water-soluble proteins from Eupolyphaga steleophaga was optimized by response surface methodology(RSM), using water-soluble proteins yield(Y) as response value. Results The test results showed that all three factors had significant effects on Y, and their optimal values were determined by three-dimensional response surface diagrams and overlaying contour plots as follows: liquid/solid ratio of 40:1, extraction time of 20 min, and p H value of 9. Conclusion Under such conditions, the measured value of Y was 11.06 %, which is close to the predicted value, which reveals that RSM is feasible for the optimization of extraction of water-soluble proteins from Eupolyphaga steleophaga.
Objective To explore the optimal extraction conditions of water-soluble proteins from Eupolyphaga steleophaga. Methods Based on the results of single-factor tests, the extraction process of water-soluble proteins from Eupolyphaga steleophaga was optimized by response surface methodology(RSM), using water-soluble proteins yield(Y) as response value. Results The test results showed that all three factors had significant effects on Y, and their optimal values were determined by three-dimensional response surface diagrams and overlaying contour plots as follows: liquid/solid ratio of 40:1, extraction time of 20 min, and p H value of 9. Conclusion Under such conditions, the measured value of Y was 11.06 %, which is close to the predicted value, which reveals that RSM is feasible for the optimization of extraction of water-soluble proteins from Eupolyphaga steleophaga.
引文
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[2]余庆阳,姚履渊,詹笑春,等.严重创伤后的代谢反应与中药治疗的临床研究[J].中国中医骨伤科杂志,2000,8(2):20-22.
[3]黎子蔚,韩雅莉,刘浩,等.地鳖纤溶活性蛋白的溶栓、溶血与抑瘤作用研究[J].2010,33(6):859-863.
[4]曹付春.地鳖虫纤溶活性先导蛋白抑制肿瘤新生血管形成与作用机制研究[D].广州:广东工业大学,2011.
[5]仲卫红,齐振熙.土鳖虫含药血清干预骨髓间充质干细胞的蛋白组学差异[J].中国组织工程研究,2013,17(1):98-105.
[6]邹玺,刘宝瑞,钱晓萍,等.土鳖虫提取液对人胃低分化腺癌细胞BGC-823的抑制作[J].时珍国医国药,2006,17(9):1695-1696.
[7]张东梅,李穗晶,韩雅莉,等.地鳖虫活性蛋白对人舌癌细胞Tea-8113的抑制作[J].时珍国医国药,2009,20(4):778-779.
[8]国家药典委员会.中华人民共和国药典(2015年版四部).北京:中国医药科技出版社,2015:96-97.