植物激素对丽格海棠组织培养快繁的影响
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摘要
根据目前丽格海棠组织培养快繁现状,研究了以MS培养基为基础附加不同种类和配比植物激素对丽格海棠组织培养中不定芽再生、不定芽继代增殖和生根的影响。结果表明:丽格海棠不定芽再生的最佳植物激素配比为MS+1mg/L 6-BA+0.1mg/L TDZ或MS+1mg/L 6-BA+0.1mg/L 2.4-D;不定芽继代增殖的最佳植物激素配比为MS+2mg/L 6-BA+0～0.1mg/L NAA;组培苗生根的最佳植物激素配比为MS+0.1～0.3mg/L IBA。
According to the present situation of tissue culture and rapid propagation of Begonia regia,the effects of different kinds and proportions of plant hormones on adventitious bud regeneration, adventitious bud subculture and rooting in tissue culture of Begonia Rieger were studied on the basis of MS medium. The results show that:The optimum phytohormone ratio for adventitious bud regeneration of Begonia Rieger was MS+1 mg/L6-BA+0.1 mg/LTDZ or MS+1 mg/L6-BA+0.1 mg/L2.4-D. The optimum ratio of plant hormones for adventitious bud subculture was MS+2 mg/L 6-BA+0-0.1 mg/L NAA. The optimum ratio of plant hormones for rooting of tissue culture seedlings was MS + 0.1-0.3 mg/L IBA.
According to the present situation of tissue culture and rapid propagation of Begonia regia,the effects of different kinds and proportions of plant hormones on adventitious bud regeneration, adventitious bud subculture and rooting in tissue culture of Begonia Rieger were studied on the basis of MS medium. The results show that:The optimum phytohormone ratio for adventitious bud regeneration of Begonia Rieger was MS+1 mg/L6-BA+0.1 mg/LTDZ or MS+1 mg/L6-BA+0.1 mg/L2.4-D. The optimum ratio of plant hormones for adventitious bud subculture was MS+2 mg/L 6-BA+0-0.1 mg/L NAA. The optimum ratio of plant hormones for rooting of tissue culture seedlings was MS + 0.1-0.3 mg/L IBA.
引文
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[9]达克东,张松,姜璐琰,等.丽格海棠离体叶片培养不定芽发生和微繁研究[J].山东农业大学学报(自然科学版),2002(01):93-95.
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[11]陈林晶,张熹涛,曹玉凤,等.丽格海棠组织培养技术的研究[J].北方园艺,2012(22):122-124.
[2]曹福亮,陈颖,桂仁意.丽格海棠组培快繁技术体系的建立及优化[J].林业科技开发,2009,23(05):82-84.
[3] 张文珠,林德钦.丽格海棠的组织培养和快速繁殖[J].广西热带农业,2005(03):9-11.
[4] 韩建军,张淑丽.丽格海棠组培中间繁殖体增殖技术的简化与优化[J].中国林副特产,2004(02):27-29.
[5]任启闯.丽格海棠离体快繁技术的研究[D].合肥:安徽农业大学,2008.
[6]文锦芬,邓明华,施卫省等.丽格海棠离体培养植株再生关键技术的研究[J].昆明理工大学学报(理工版),2006(02):111-113+124.
[7]韩超.丽格海棠组织培养技术体系研究[D].保定:河北农业大学,2005.
8]张云峰,李静,邢宇俊,等.丽格海棠再生体系建立的研究[J].安徽农业科学,2007(03):663-664.
[9]达克东,张松,姜璐琰,等.丽格海棠离体叶片培养不定芽发生和微繁研究[J].山东农业大学学报(自然科学版),2002(01):93-95.
[10]耿明清.丽格海棠叶片的组织培养[J].辽宁师专学报(自然科学版),2012(01):99-101.
[11]陈林晶,张熹涛,曹玉凤,等.丽格海棠组织培养技术的研究[J].北方园艺,2012(22):122-124.