一定光量子通量密度下不同倍性谷子光合因子日响应拟合模型的构建
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摘要
为研究不同倍性谷子光合因子日响应差异,构建光量子通量密度确定的条件(1 200μmol/(m~2·s))下各生理指标之间的互作模型。以谷子栽培种普通二倍体(2x)晋谷21号及其同源四倍体(4x)诱变株系为材料,收集2013—2015年的光合因子数据,运用spss.18.0与microsoft mathematics v4.0对不同倍性谷子光合因子的响应关系进行相关分析和通径分析,得到参数间因变关系的决策系数(R~2)及模式方程。结果表明:1)不同倍性谷子叶片净光合速率(P_n)与胞间CO_2浓度(C_i)、蒸腾速率(E)和叶温(T)的关系较密切。剔除其他因子干扰,不同倍性谷子净光合速率均与叶温直接相关。叶温主要通过胞间CO_2浓度和蒸腾速率影响作物的净光合速率。空气相对湿度(RH)对净光合速率的直接影响较小,而气孔导度(G_s)对净光合速率的影响主要是通过影响胞间CO_2浓度来实现,其影响因子则主要是空气相对湿度。2)二倍体谷子光合因子间线性关系较为明显,而四倍体更多是相关因子协同作用的结果。本研究获得的模型可以准确拟合不同倍性谷子在一定光量子通量密度下光合因子的日响应。
To study photosynthetic factors responding to diurnal variation of millet with different ploidies,the interaction models of physiological indices on PFD(photon flux density)of 1 200μmol/(m~2·s)were constructed.Cultivar of Jingu 21(2 x)and its autotetraploid(4 x)were taking as study materials.Data were collected from 2013 to 2015.Correlation analysis and stepwise regression analysis were used to explore interactions.Decision coefficients(R~2)of photosynthesis factors with environmental factors,which were analyzed by SPSS.18.0 and Microsoft Mathematics V4.0.The results showed that:1)Net photosynthetic rate(P_n)was closely related with intercellular CO_2 concentration(C_i),evaporation rate(E)and leaf temperature(T)of different ploidies.Apart from other factors,net photosynthetic rate had direct relation with leaf temperature.Intercellular CO_2 concentration effected net photosynthetic rate by leaf temperature.The relative humidity of air displayed little correlation with net photosynthetic rate.The stomatal conductance(G_s)effect netphotosynthetic rate by intercellular CO_2 concentration,and its main impact factor is relative humidity of air.2)The linear interrelation of photosynthetic factors is significant for diploid millet,but it is the result from synergistic effect of different factors for autotetraploid.In conclusion,the models constructed in this study could simulate the photosynthetic factors responding to diurnal variation of millets having different ploidies.
To study photosynthetic factors responding to diurnal variation of millet with different ploidies,the interaction models of physiological indices on PFD(photon flux density)of 1 200μmol/(m~2·s)were constructed.Cultivar of Jingu 21(2 x)and its autotetraploid(4 x)were taking as study materials.Data were collected from 2013 to 2015.Correlation analysis and stepwise regression analysis were used to explore interactions.Decision coefficients(R~2)of photosynthesis factors with environmental factors,which were analyzed by SPSS.18.0 and Microsoft Mathematics V4.0.The results showed that:1)Net photosynthetic rate(P_n)was closely related with intercellular CO_2 concentration(C_i),evaporation rate(E)and leaf temperature(T)of different ploidies.Apart from other factors,net photosynthetic rate had direct relation with leaf temperature.Intercellular CO_2 concentration effected net photosynthetic rate by leaf temperature.The relative humidity of air displayed little correlation with net photosynthetic rate.The stomatal conductance(G_s)effect netphotosynthetic rate by intercellular CO_2 concentration,and its main impact factor is relative humidity of air.2)The linear interrelation of photosynthetic factors is significant for diploid millet,but it is the result from synergistic effect of different factors for autotetraploid.In conclusion,the models constructed in this study could simulate the photosynthetic factors responding to diurnal variation of millets having different ploidies.
引文
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[9]郝兴宇,李萍,林而达,仝乘风,魏强,巫国栋,董小刚.大气CO2浓度升高对谷子生长发育与光合生理的影响[J].核农学报,2010,24(3):589-593Hao X H,Li P,Lin E D,Tong C F,Wei Q,Wu G D,Dong X G.Effects of air CO2Enrichment on growth and photosynthetic physiology of millet[J].Nuclear Agricultural Sciences,2010,24(3):589-593(in Chinese)
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[12]Han J,Xie H,Sun Q P,Wang J,Lu M,Wang W X,Guo E H,Pan J B.Bioinformatic identification and experimental validation of mirnas from foxtail millet(Setaria italica)[J].Gene,2014,546(2):367-377
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[15]Chaves M M,Flexas J,Pinheiro C.Photosynthesis under drought and salt stress:Regulation mechanisms from whole plant to cell[J].Annals of Botany,2009,103(4):551-560
[16]Hussain S,Luro F,Costantino G,Ollitrault P,Morillon R.Physiological analysis of salt stress behaviour of citrus species and genera:Low chloride accumulation as an indicator of salt tolerance[J].South African Journal of Botany,2012,81(5):103-112
[17]Kalaji H M,Govindjee,Bosa K,Koscielniak J,ZukGolaszewska K.Effects of salt stress on photosystem II efficiency and CO2assimilation of two Syrian barley landraces[J].Environmental and Experimental Botany,2011,73(4):64-72
[18]Kudoh H,Sonoike K.Irreversible damage to photosystem I by chilling in the light:Cause of the degradation of chlorophyll after returning to normal growth temperature[J].Planta,2002,215(4):541-548
[19]Chao D Y,Dilkes B,Luo H B,Douglas A,Yakubova E,Lahner B,Salt D E.Polyploids exhibit higher potassium uptake and salinity tolerance in Arabidopsis[J].Science,2013,341(6146):658-659
[20]Wang Z M,Wang M Y,Liu L,Meng F J.Physiological and proteomic responses of diploid and tetraploid black locust(Robinia pseudoacacia L)subjected to salt stress[J].International Journal of Molecular Sciences,2013,14(10):20299-20325
[21]Saleh B,Allario T,Dambier D,Ollitrault P,Morillon R.Tetraploid citrus rootstocks are more tolerant to salt stress than diploid[J].Comptes Rendus Biologies,2008,331(9):703-710
[22]Yan K,Chen W,He X Y,Zhang G Y,Xu S,Wang L L.Responses of photosynthesis,lipid peroxidation and antioxidant system in leaves of Quercus mongolica to elevated O3[J].Environmental and Experimental Botany,2010,69(2):198-204
[23]Strasser R J,Tsimilli-Michael M,Qiang S,Goltsev V.Simultaneous in vivo recording of prompt and delayed fluorescence and 820nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis[J].Biochimica et Biophysica Acta-Bioenergetics,2010,1797(3):1313-1326
[24]Yan K,Chen P,Shao H,Zhao S,Zhang L,Zhang L,Xu G,Sun J.Responses of photosynthesis and photosystemⅡto higher temperature and salt stress in sorghum[J].Journal of Agronomy and Crop Science,2012,198(3):218-226
[25]叶子飘.光合作用对光和CO2响应模型的研究进展[J].植物生态学报,2010,34(6):727-740Ye Z P.A review on modeling of responses of photosynthesis to light and CO2[J].Chinese Journal of Plant Ecology,2010,34(6):727-740(in Chinese)
[26]张治安,李亚东,徐晨,徐克章.4种不同类型越桔叶片光合作用温度特性的比较研究[J].吉林农业大学学报,1999,21(4):16-19Zhang Z A,Li Y D,Xu C,Xu K Z.Photosynthetic temperature characteristic in four different types of blueberry leaves[J].Journal of Jilin Agricultural University,1999,21(4):16-19(in Chinese)
[27]李亚东,张治安,吴林.红豆越桔光合作用特性的研究[J].园艺学报,1996,23(1):86-88Li Y D,Zhang Z A,Wu L.Studies on the photosynthetic characteristics of lingonberry[J].Acta Horticulturae Sinica,1996,23(1):86-88(in Chinese)
[28]徐克章,王英典,徐惠风,张治安.高粱叶片光合作用特性的研究.吉林农业大学学报,1999,21(3):1-6Xu K Z,Wang Y D,Xu H F,Zhang Z A.Studies on the photosynthetic characteristic in leaves of sorghum[J].Journal of Jilin Agricultural University,1999,21(3):1-6(in Chinese)
[29]宋小园,朱仲元,刘艳伟,赵宏瑾,通径分析在SPSS逐步线性回归中的实现[J],干旱区研究,2006:33(1):108-113Song X Y,Zhu Z Y,Liu Y W,Zhao H J,Application of path analysis in stepwise linear regression SPSS[J].Arid Zone Research,2006:33(1):108-113(in Chinese)
[30]刘小莉,刘飞虎.花卉育种技术研究进展[J].亚热带植物科学,2003,32(2):64-68Liu X L,Liu F H.The research progress about breeding technology of flowers plant[J].Subtropical Plant Science,2003,32(2):64-68(in Chinese)
[31]庄文庆.药用植物育种学[M].北京:农业出版社,1993.103-110Zhuang W Q.Medicinal Plant Breeding[M].Peking:Agriculture Publishing House,1993.103-110(in Chinese)
[32]刘惠吉.蔬菜作物多倍体研究及应用[J].长江蔬菜,1995,31(3):3-5Liu H P.Research and application about vegetable plant[J].Changjiang Vegetable Plant,1995,31(3):3-5(in Chinese)
[33]Wendel J F.Genome evolution in ployploids[J].Plant Molecular Biology,2000,42(1):225-249
[34]Pickett F B,Meeks-Wagner D R.Seeing double:Appreciating genetic re-dundancy[J].Plant Cell,1995,7(6):1347-1356
[2]廖建雄,王根轩.谷子叶片光合速率日变化及水分利用效率[J].植物生理学报,1999,25(4):362-368Liao J X,Wang G X.The diurnal variations of photosynthetic rate and water use efficiency in Setaria italica leaves[J].Acta Phytophysiologica Sinica,1999,25(4):362-368(in Chinese)
[3]徐克章,张美善.恒定环境条件下西洋参叶片光合作用日变化[J].吉林农业大学学报,2003,25(2):134-138Xu K Z,Zhang M S.Diurnal changes of photosynthesis of Panax quinquefolium leaves under invariable environmental conditions[J].Journal of Jilin Agricultural University,2003,25(2):134-138(inChinese)
[4]童贯和.不同供钾水平对小麦旗叶光合速率日变化的影响[J].植物生态学报,2004,28(4):547-553Tong G H.The effect different levels of potassium nutrition on the diurnal variation of photosynthetic rates of wheat flag leaves[J].Acta Phytoecologica Sinica,2004,28(4):547-553(in Chinese)
[5]李萍萍,胡永光,赵玉国,毛罕平.叶用莴苣温室栽培单株光合作用日变化规律[J].园艺学报,2001,28(3):240-245Li P P,Hu Y G,Zhao Y G,Mao H P.A study on daily variation of photosynthesis in greenhouse butter-head lettuce[J].Acta Horticulturae Sinica,2001,28(3):240-245(in Chinese)
[6]贾冠清,刁现民.谷子(Setaria italica(L)P Beauv)作为功能基因组研究模式植物的发展现状及趋势[J].生命科学,2017,29(3):293-297Jia G Q,Diao X M.Current status and perspectives of researches on foxtail millet(Setaria italica(L)P Beauv.):A potential model of plant functional genomics studies[J].Chinese Bulletin of Life Sciences,2017,29(3):293-297(in Chinese)
[7]刘子会,张红梅,张艳敏,李国良,郭秀林.灌浆期杂交谷子旗叶的光合特性[J].西北农业学报,2012,21(11):60-64Liu Z H,Zhang H M,Zhang Y M,Li G L,Guo X L.Photosynthetic characteristics of hybrid millet at grain filling stage[J],Acta Agricultuare Boreali-occidentalis Sinica,2012,21(11):60-64(in Chinese)
[8]Feng X L,Zhao Z H,Wang X M,Qiu F C,Song G L,Wang D Q,Su X,Zhang X L,Wang F.Recent research progress in Foxtail Millet(Setaria italica)[J].Agricultural Science&Technology,2014,15(4):564-570
[9]郝兴宇,李萍,林而达,仝乘风,魏强,巫国栋,董小刚.大气CO2浓度升高对谷子生长发育与光合生理的影响[J].核农学报,2010,24(3):589-593Hao X H,Li P,Lin E D,Tong C F,Wei Q,Wu G D,Dong X G.Effects of air CO2Enrichment on growth and photosynthetic physiology of millet[J].Nuclear Agricultural Sciences,2010,24(3):589-593(in Chinese)
[10]Dujardin M.,Hanna WW.Fertility improvement in tetraploid pearl millet[J].Euphytica,1989,42(3):285-289
[11]Benabdelmouna A,Shi Y,Abirached-Darmency M,Dar-mency H.Genomic in situ hybridization(GISH)discrimi nates between the A and the B genomes in diploid and tetraploid Setaria species[J].Chinese Journal of Oil Crop Scieves,2002,44(1):685-690
[12]Han J,Xie H,Sun Q P,Wang J,Lu M,Wang W X,Guo E H,Pan J B.Bioinformatic identification and experimental validation of mirnas from foxtail millet(Setaria italica)[J].Gene,2014,546(2):367-377
[13]Rozema J,Flowers T.Ecology crops for a salinized world[J].Science,2008,322(5907):1478-1480
[14]Munns R,Tester M.Mechanisms of salinity tolerance[J].Annual Review of Plant Biology,2008,59(1):651-681
[15]Chaves M M,Flexas J,Pinheiro C.Photosynthesis under drought and salt stress:Regulation mechanisms from whole plant to cell[J].Annals of Botany,2009,103(4):551-560
[16]Hussain S,Luro F,Costantino G,Ollitrault P,Morillon R.Physiological analysis of salt stress behaviour of citrus species and genera:Low chloride accumulation as an indicator of salt tolerance[J].South African Journal of Botany,2012,81(5):103-112
[17]Kalaji H M,Govindjee,Bosa K,Koscielniak J,ZukGolaszewska K.Effects of salt stress on photosystem II efficiency and CO2assimilation of two Syrian barley landraces[J].Environmental and Experimental Botany,2011,73(4):64-72
[18]Kudoh H,Sonoike K.Irreversible damage to photosystem I by chilling in the light:Cause of the degradation of chlorophyll after returning to normal growth temperature[J].Planta,2002,215(4):541-548
[19]Chao D Y,Dilkes B,Luo H B,Douglas A,Yakubova E,Lahner B,Salt D E.Polyploids exhibit higher potassium uptake and salinity tolerance in Arabidopsis[J].Science,2013,341(6146):658-659
[20]Wang Z M,Wang M Y,Liu L,Meng F J.Physiological and proteomic responses of diploid and tetraploid black locust(Robinia pseudoacacia L)subjected to salt stress[J].International Journal of Molecular Sciences,2013,14(10):20299-20325
[21]Saleh B,Allario T,Dambier D,Ollitrault P,Morillon R.Tetraploid citrus rootstocks are more tolerant to salt stress than diploid[J].Comptes Rendus Biologies,2008,331(9):703-710
[22]Yan K,Chen W,He X Y,Zhang G Y,Xu S,Wang L L.Responses of photosynthesis,lipid peroxidation and antioxidant system in leaves of Quercus mongolica to elevated O3[J].Environmental and Experimental Botany,2010,69(2):198-204
[23]Strasser R J,Tsimilli-Michael M,Qiang S,Goltsev V.Simultaneous in vivo recording of prompt and delayed fluorescence and 820nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis[J].Biochimica et Biophysica Acta-Bioenergetics,2010,1797(3):1313-1326
[24]Yan K,Chen P,Shao H,Zhao S,Zhang L,Zhang L,Xu G,Sun J.Responses of photosynthesis and photosystemⅡto higher temperature and salt stress in sorghum[J].Journal of Agronomy and Crop Science,2012,198(3):218-226
[25]叶子飘.光合作用对光和CO2响应模型的研究进展[J].植物生态学报,2010,34(6):727-740Ye Z P.A review on modeling of responses of photosynthesis to light and CO2[J].Chinese Journal of Plant Ecology,2010,34(6):727-740(in Chinese)
[26]张治安,李亚东,徐晨,徐克章.4种不同类型越桔叶片光合作用温度特性的比较研究[J].吉林农业大学学报,1999,21(4):16-19Zhang Z A,Li Y D,Xu C,Xu K Z.Photosynthetic temperature characteristic in four different types of blueberry leaves[J].Journal of Jilin Agricultural University,1999,21(4):16-19(in Chinese)
[27]李亚东,张治安,吴林.红豆越桔光合作用特性的研究[J].园艺学报,1996,23(1):86-88Li Y D,Zhang Z A,Wu L.Studies on the photosynthetic characteristics of lingonberry[J].Acta Horticulturae Sinica,1996,23(1):86-88(in Chinese)
[28]徐克章,王英典,徐惠风,张治安.高粱叶片光合作用特性的研究.吉林农业大学学报,1999,21(3):1-6Xu K Z,Wang Y D,Xu H F,Zhang Z A.Studies on the photosynthetic characteristic in leaves of sorghum[J].Journal of Jilin Agricultural University,1999,21(3):1-6(in Chinese)
[29]宋小园,朱仲元,刘艳伟,赵宏瑾,通径分析在SPSS逐步线性回归中的实现[J],干旱区研究,2006:33(1):108-113Song X Y,Zhu Z Y,Liu Y W,Zhao H J,Application of path analysis in stepwise linear regression SPSS[J].Arid Zone Research,2006:33(1):108-113(in Chinese)
[30]刘小莉,刘飞虎.花卉育种技术研究进展[J].亚热带植物科学,2003,32(2):64-68Liu X L,Liu F H.The research progress about breeding technology of flowers plant[J].Subtropical Plant Science,2003,32(2):64-68(in Chinese)
[31]庄文庆.药用植物育种学[M].北京:农业出版社,1993.103-110Zhuang W Q.Medicinal Plant Breeding[M].Peking:Agriculture Publishing House,1993.103-110(in Chinese)
[32]刘惠吉.蔬菜作物多倍体研究及应用[J].长江蔬菜,1995,31(3):3-5Liu H P.Research and application about vegetable plant[J].Changjiang Vegetable Plant,1995,31(3):3-5(in Chinese)
[33]Wendel J F.Genome evolution in ployploids[J].Plant Molecular Biology,2000,42(1):225-249
[34]Pickett F B,Meeks-Wagner D R.Seeing double:Appreciating genetic re-dundancy[J].Plant Cell,1995,7(6):1347-1356