气—质联用法同时检测高脂食品中14种光引发剂
|
摘要
建立气相色谱-质谱联用仪测定高脂食品中14种光引发剂的方法。将均质后的试样经丙酮-正己烷(体积比2:8) 20℃提取15 min后,振荡离心,上清液采用HLB管进行净化,收集洗脱液,并以PSA萃取管进一步净化。离心后提取上机。当加标水平为0. 000 2~0. 001 0 mg/kg时的回收率为73. 0%~92. 4%,相对标准偏差为4. 1%~8. 9%,检出限为0. 02~0. 20μg/kg。该方法操作快捷,技术参数均满足GB/T 27404—2008附录F中的要求,适用于高脂食品中14种光引发剂的同时分析。
To establish a GC-MS method for determination of 14 photo-initiators in fatty food. The homogeneous samples were extracted by acetonehexane(2:8,v: v) for 15 min at room temperature. The supernatant was purified by HLB column,and the eluent was then collected,which was further purified by PSA. The purified eluent was centrifuged and determined by GC-MS. The recoveries were between 73. 0% ~ 92. 4% at spiked levels of 0. 000 2 ~ 0. 001 0 mg/kg,and the relative standard deviation of the methods were 4. 1% ~ 8. 9%,with the LOD ranging from0. 02 ~ 0. 20 μg/kg. It was in accordance with the requirement of technical parameters for analysis with fast and simple determination and high sensitivity. It was suitable for the simultaneous determination of 14 photoinitiators in fatty food.
To establish a GC-MS method for determination of 14 photo-initiators in fatty food. The homogeneous samples were extracted by acetonehexane(2:8,v: v) for 15 min at room temperature. The supernatant was purified by HLB column,and the eluent was then collected,which was further purified by PSA. The purified eluent was centrifuged and determined by GC-MS. The recoveries were between 73. 0% ~ 92. 4% at spiked levels of 0. 000 2 ~ 0. 001 0 mg/kg,and the relative standard deviation of the methods were 4. 1% ~ 8. 9%,with the LOD ranging from0. 02 ~ 0. 20 μg/kg. It was in accordance with the requirement of technical parameters for analysis with fast and simple determination and high sensitivity. It was suitable for the simultaneous determination of 14 photoinitiators in fatty food.
引文
[1]金养智.光引发剂的进展[J].影像技术,2011,23(3):8-18.
[2]韦星船,杨前程,李国荣,等.紫外光固化水性光引发剂的研究现状及进展[J].电镀与涂饰,2014,33(14):587-591.
[3]HOUWE K V D,VELDE S V D,EVRARD C,et al.Evaluation of the migration of 15 photo-initiators from cardboard packaging into Tenaxusing ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)[J].Food Addit Contam Part AChem Anal Control Expo Risk Assess,2014,31(4):767-775.
[4]邓晓军,郭德华,李波,等.气相色谱-质谱法测定乳制品中光引发剂异丙基硫杂蒽酮的残留量[J].色谱,2007,25(1):39-42.
[5]RHODES M C,BUCHER J R,PPECKHAM J C,et al.Carcinogenesis studies of benzophenone in rats and mice[J].Food and Chemical Toxicology,2007,45(5):843-851.
[6]COOK N,FREEMAN S.Report of 19 cases of photoallergic contact dermatitis to sunscreens seen at the Skin and Cancer Foundation[J].Australasian Journal of Dermatology,2015,42(4):257-259.
[7]李中皓,吴帅宾,刘珊珊,等.超高效合相色谱法快速检测纸质印刷包装材料中10种受限制光引发剂[J].分析化学,2013,41(12):1 817-1 824.
[8]张居舟,尚光志,蔡荟梅,等.基质分散固相萃取-气相色谱-质谱联用法测定塑料食品包装材料中9种光引发剂[J].食品工业科技,2015,36(17):269-274.
[9]SANCHES-SILVA A,PASTORELLI S,CRUZ J M,et al.Development of a multimethod for the determination of photoinitiators in beverage packaging[J].Journal of Food Science,2010,73(2):C92-C99.
[10]刘艳,张强,顾华,等.气相色谱-质谱同时测定食品包装材料中9种光引发剂[J].分析科学学报,2017(6):812-816.
[11]张耀海,焦必宁,周志钦.气相色谱-串联质谱法结合QuEChERS方法快速检测软包装饮料中8种光引发剂[J].分析化学,2012,40(10):1 536-1 542.
[12]邓晓军,郭德华,李波,等.气相色谱-质谱法测定乳制品中光引发剂异丙基硫杂蒽酮的残留量[J].色谱,2007,25(1):39-42.
[13]SAGRATIINI G,CAPRIOLI G,CRISTALLI G,et al.Determination of ink photoinitiators in packaged beverages by gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry[J].Journal of Chromatography A,2008,1 194(2):213-220.
[14]MATOVSKA K.Evaluation of common organic solvents for gas chromatographic analysis and stability of multiclass pesticide residues[J].Journal of Chromatography A,2004,1 040(2):259-272.
[15]张建莹,沈金灿,岳振峰,等.液相色谱-串联质谱法测定牛奶中11种光引发剂残留量[J].江西农业学报,2013(7):94-97.
[16]ROTHENBACKER T,FUGEL M B D.2-Isopropylthioxan-thone(2-ITX)in food and food packaging materials on the German market[J].Food Additives&Contaminants,2007,24(4):438-444.
[17]ZHANG Y,JIAO B.Dispersive Liquid-Liquid Microextraction Combined With Micellar Electrokinetic Chromatography for the Determination of Some Photoinitiators in Fruit Juice[J].Food Analytical Methods,2015,8(4):973-981.
[18]李中皓,范子彦,边照阳,等.分散固相萃取净化气相色谱-质谱联用法快速检测纸质包装材料中18种光引发剂[J].分析化学,2013,41(9):1 334-1 340.
[2]韦星船,杨前程,李国荣,等.紫外光固化水性光引发剂的研究现状及进展[J].电镀与涂饰,2014,33(14):587-591.
[3]HOUWE K V D,VELDE S V D,EVRARD C,et al.Evaluation of the migration of 15 photo-initiators from cardboard packaging into Tenaxusing ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)[J].Food Addit Contam Part AChem Anal Control Expo Risk Assess,2014,31(4):767-775.
[4]邓晓军,郭德华,李波,等.气相色谱-质谱法测定乳制品中光引发剂异丙基硫杂蒽酮的残留量[J].色谱,2007,25(1):39-42.
[5]RHODES M C,BUCHER J R,PPECKHAM J C,et al.Carcinogenesis studies of benzophenone in rats and mice[J].Food and Chemical Toxicology,2007,45(5):843-851.
[6]COOK N,FREEMAN S.Report of 19 cases of photoallergic contact dermatitis to sunscreens seen at the Skin and Cancer Foundation[J].Australasian Journal of Dermatology,2015,42(4):257-259.
[7]李中皓,吴帅宾,刘珊珊,等.超高效合相色谱法快速检测纸质印刷包装材料中10种受限制光引发剂[J].分析化学,2013,41(12):1 817-1 824.
[8]张居舟,尚光志,蔡荟梅,等.基质分散固相萃取-气相色谱-质谱联用法测定塑料食品包装材料中9种光引发剂[J].食品工业科技,2015,36(17):269-274.
[9]SANCHES-SILVA A,PASTORELLI S,CRUZ J M,et al.Development of a multimethod for the determination of photoinitiators in beverage packaging[J].Journal of Food Science,2010,73(2):C92-C99.
[10]刘艳,张强,顾华,等.气相色谱-质谱同时测定食品包装材料中9种光引发剂[J].分析科学学报,2017(6):812-816.
[11]张耀海,焦必宁,周志钦.气相色谱-串联质谱法结合QuEChERS方法快速检测软包装饮料中8种光引发剂[J].分析化学,2012,40(10):1 536-1 542.
[12]邓晓军,郭德华,李波,等.气相色谱-质谱法测定乳制品中光引发剂异丙基硫杂蒽酮的残留量[J].色谱,2007,25(1):39-42.
[13]SAGRATIINI G,CAPRIOLI G,CRISTALLI G,et al.Determination of ink photoinitiators in packaged beverages by gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry[J].Journal of Chromatography A,2008,1 194(2):213-220.
[14]MATOVSKA K.Evaluation of common organic solvents for gas chromatographic analysis and stability of multiclass pesticide residues[J].Journal of Chromatography A,2004,1 040(2):259-272.
[15]张建莹,沈金灿,岳振峰,等.液相色谱-串联质谱法测定牛奶中11种光引发剂残留量[J].江西农业学报,2013(7):94-97.
[16]ROTHENBACKER T,FUGEL M B D.2-Isopropylthioxan-thone(2-ITX)in food and food packaging materials on the German market[J].Food Additives&Contaminants,2007,24(4):438-444.
[17]ZHANG Y,JIAO B.Dispersive Liquid-Liquid Microextraction Combined With Micellar Electrokinetic Chromatography for the Determination of Some Photoinitiators in Fruit Juice[J].Food Analytical Methods,2015,8(4):973-981.
[18]李中皓,范子彦,边照阳,等.分散固相萃取净化气相色谱-质谱联用法快速检测纸质包装材料中18种光引发剂[J].分析化学,2013,41(9):1 334-1 340.