拟南芥光敏色素作用因子PIF3节水抗旱功能分析
|
摘要
光敏色素作用因子(PIFs)在植物生长发育和信号调控中发挥枢纽作用。为构建拟南芥PIFs转录因子PIF3的过表达载体,以蘸花法转化拟南芥,筛选获得过表达纯合株系,结合PIF3的突变体材料,对PIF3的节水抗旱功能进行分析。通过节水抗旱表型观察、离体叶片失水率测定、扫描电镜分析气孔等试验,发现拟南芥PIFs转录因子PIF3通过调节气孔开闭影响蒸腾,具有节水抗旱的功能。
Phytochrome-interacting factors (PIFs),as cellular signaling hubs,play apivotal role in plant growth and development.In this study,an over-expression vector of PIF3 was constructed and transformed into Arabidopsis.The over-expression transgenic and mutant plant of PIF3 were used to analyze the function of water-saving and drought-resistance of PIF3 based on the transpiration phenotype observation,water loss rate of leaf measurement and stomatal observation in Arabidopsis.These observations suggest that PIF3 can act as stomatal regulator and plays a pivotal role in transpiration restriction mechanism that functions as an adaptation to drought stress.
Phytochrome-interacting factors (PIFs),as cellular signaling hubs,play apivotal role in plant growth and development.In this study,an over-expression vector of PIF3 was constructed and transformed into Arabidopsis.The over-expression transgenic and mutant plant of PIF3 were used to analyze the function of water-saving and drought-resistance of PIF3 based on the transpiration phenotype observation,water loss rate of leaf measurement and stomatal observation in Arabidopsis.These observations suggest that PIF3 can act as stomatal regulator and plays a pivotal role in transpiration restriction mechanism that functions as an adaptation to drought stress.
引文
[1]TOLEDO-ORTIZ G,HUQ E,QUAIL P H.The Arabidopsis basic/helix-loop-helix transcription factor family[J].Plant Cell,2003,15(8):1749-1770.
[2]CASTILLON A,SHEN H,HUQ E.Phytochrome interacting factors:central players in phytochrome-mediated light signaling networks[J].Trends Plant Sci,2007,12(1):514-521.
[3]LEIVAR P,QUAIL P H.PIFs:pivotal components in a cellular signaling hub[J].Trends Plant Sci,2011,16(1):19-28.
[4]LEIVAR P,MONTE E.PIFs:systems Integrators in plant development[J].Plant Cell,2014,26(1):56-78.
[5]江薇,肖宁,陆怡,等.植物光敏色素作用因子PIFs的生物学功能[J].植物生理学报,2014,50(6):698-706.
[6]任小芸,吴美琴,陈建民,等.光敏色素作用因子PIFs参与植物激素信号转导的分子机制[J].植物生理学报,2016,52(10):1466-1473.
[7]STANELONI R J,RODRIGUEZ-BATILLER M J,CASAL J J.Abscisic acid,high-light,and oxidative stress down-regulate a photosynthetic gene via a promoter motif not involved in phytochrome-mediated transcriptional regulation[J].Mol Plant,2008,(1):75-83.
[8]BOCCALANDRO H E,RUGNONE M L,MORENO J E,et al.Phytochrome B enhances photosynthesis at the expense of water-use efficiency in Arabidopsis[J].Plant Physiol,2009,150(2):1083-1092.
[9]BOGGS J Z,LOEWY K,BIBEE K,et al.Phytochromes influence stomatal conductance plasticity in Arabidopsis thaliana[J].Plant Growth Regul,2010,60(2):77-81.
[10]WANG F F,LIAN H L,KANG C Y,et al.Phytochrome B is involved in mediating red light-induced stomatal opening in Arabidopsis thaliana[J].Mol Plant,2010,(3):246-259.
[11]LIU J,ZHANG F,ZHOU J J,et al.Phytochrome B control of total leaf area and stomatal density affects drought tolerance in rice[J].Plant Mol Biol,2012,78(3):289-300.
[12]TODAKA D,NAKASHIMA K,MARUYAMA K et al.Rice phytochrome-interacting factor-like protein OsPIL1functions as a key regulator of internode elongation and induces a morphological response to drought stress[J].Proc Natl Acad Sci USA,2012,109(39):15947-15952.
[13]CORDEIRO A M,FIGUEIREDO D D,TEPPERMAN J,et al.Rice phytochrome-interacting factor protein OsPIF14represses OsDREB1Bgene expression through an extended N-box and interacts preferentially with the active form of phytochrome B[J].BBA Gene Regul Mech,2016,1859(2):393-404.
[14]GAO Y,JIANG W,DAI Y,et al.A maize phytochrome-interacting factor 3improves drought and salt stress tolerance in rice[J].Plant Mol Biol,2015,87(4/5):413-428.
[15]GAO Y,WU M Q,ZHANG M J,et al.A maize phytochrome-interacting factors protein ZmPIF1enhances drought tolerance by inducing stomatal closure and improves grain yield in Oryza sativa[J].Plant Biotechnol J,2018,16(7):1375-1387.
[16]NEMHAUSER J L,HONG F,CHORY J.Different plant hormones regulate similar processes through largely nonoverlapping transcriptional responses[J].Cell,2006,126(3):467-475.
[17]PAIK I,KATHARE P K,KIM J I,et al.Expanding roles of PIFs in signal integration from multiple processes[J].Molecular Plant,2017,10(8):1035-1046.
[18]SCHROEDER J I,ALLEN G J,HUGOUVIEUX V,et al.Guard cell signal transduction[J].Annu Rev Plant Phys,2001,52:627-658.
[19]MURATA Y,MORI IC,MUNEMASA S.Diverse stomatal signaling and the signal integration mechanism[J].Annu Rev Plant Biol,2015,66:369-392.
[20]CASSON S A,FRANKLIN K A,GRAY J E,et al.Phytochrome B and PIF4regulate stomatal development in response to light quantity[J].Curr Biol,2009,19(3):229-234.
[2]CASTILLON A,SHEN H,HUQ E.Phytochrome interacting factors:central players in phytochrome-mediated light signaling networks[J].Trends Plant Sci,2007,12(1):514-521.
[3]LEIVAR P,QUAIL P H.PIFs:pivotal components in a cellular signaling hub[J].Trends Plant Sci,2011,16(1):19-28.
[4]LEIVAR P,MONTE E.PIFs:systems Integrators in plant development[J].Plant Cell,2014,26(1):56-78.
[5]江薇,肖宁,陆怡,等.植物光敏色素作用因子PIFs的生物学功能[J].植物生理学报,2014,50(6):698-706.
[6]任小芸,吴美琴,陈建民,等.光敏色素作用因子PIFs参与植物激素信号转导的分子机制[J].植物生理学报,2016,52(10):1466-1473.
[7]STANELONI R J,RODRIGUEZ-BATILLER M J,CASAL J J.Abscisic acid,high-light,and oxidative stress down-regulate a photosynthetic gene via a promoter motif not involved in phytochrome-mediated transcriptional regulation[J].Mol Plant,2008,(1):75-83.
[8]BOCCALANDRO H E,RUGNONE M L,MORENO J E,et al.Phytochrome B enhances photosynthesis at the expense of water-use efficiency in Arabidopsis[J].Plant Physiol,2009,150(2):1083-1092.
[9]BOGGS J Z,LOEWY K,BIBEE K,et al.Phytochromes influence stomatal conductance plasticity in Arabidopsis thaliana[J].Plant Growth Regul,2010,60(2):77-81.
[10]WANG F F,LIAN H L,KANG C Y,et al.Phytochrome B is involved in mediating red light-induced stomatal opening in Arabidopsis thaliana[J].Mol Plant,2010,(3):246-259.
[11]LIU J,ZHANG F,ZHOU J J,et al.Phytochrome B control of total leaf area and stomatal density affects drought tolerance in rice[J].Plant Mol Biol,2012,78(3):289-300.
[12]TODAKA D,NAKASHIMA K,MARUYAMA K et al.Rice phytochrome-interacting factor-like protein OsPIL1functions as a key regulator of internode elongation and induces a morphological response to drought stress[J].Proc Natl Acad Sci USA,2012,109(39):15947-15952.
[13]CORDEIRO A M,FIGUEIREDO D D,TEPPERMAN J,et al.Rice phytochrome-interacting factor protein OsPIF14represses OsDREB1Bgene expression through an extended N-box and interacts preferentially with the active form of phytochrome B[J].BBA Gene Regul Mech,2016,1859(2):393-404.
[14]GAO Y,JIANG W,DAI Y,et al.A maize phytochrome-interacting factor 3improves drought and salt stress tolerance in rice[J].Plant Mol Biol,2015,87(4/5):413-428.
[15]GAO Y,WU M Q,ZHANG M J,et al.A maize phytochrome-interacting factors protein ZmPIF1enhances drought tolerance by inducing stomatal closure and improves grain yield in Oryza sativa[J].Plant Biotechnol J,2018,16(7):1375-1387.
[16]NEMHAUSER J L,HONG F,CHORY J.Different plant hormones regulate similar processes through largely nonoverlapping transcriptional responses[J].Cell,2006,126(3):467-475.
[17]PAIK I,KATHARE P K,KIM J I,et al.Expanding roles of PIFs in signal integration from multiple processes[J].Molecular Plant,2017,10(8):1035-1046.
[18]SCHROEDER J I,ALLEN G J,HUGOUVIEUX V,et al.Guard cell signal transduction[J].Annu Rev Plant Phys,2001,52:627-658.
[19]MURATA Y,MORI IC,MUNEMASA S.Diverse stomatal signaling and the signal integration mechanism[J].Annu Rev Plant Biol,2015,66:369-392.
[20]CASSON S A,FRANKLIN K A,GRAY J E,et al.Phytochrome B and PIF4regulate stomatal development in response to light quantity[J].Curr Biol,2009,19(3):229-234.