五磨饮对脾虚气滞证功能性消化不良大鼠的干预作用
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摘要
目的研究五磨饮对脾虚气滞证功能性消化不良(FD)大鼠的干预作用。方法 70只雌性SD大鼠随机选出10只作为正常组,其余采用"慢性束缚+游泳+饮食不节"复合因素法制备脾虚气滞证FD模型。48只造模成功大鼠随机分为模型组、多潘立酮组、柴芍六君子汤组、五磨饮高剂量组、五磨饮中剂量组、五磨饮低剂量组,每组8只。除正常组及模型组给予等体积纯净水外,其余大鼠灌胃给药干预14 d。记录大鼠食量和体质量,测定小肠推进率,检测胃窦及下丘脑组织诱导型一氧化氮合酶(iNOS)、P物质(SP)、5-羟色胺(5-HT)表达。结果与模型组比较,给药第5、10、15天,正常组、各给药组大鼠食量显著增加(P <0.05或P <0.01)。与模型组比较,给药第5天,正常组、多潘立酮组大鼠体质量显著增加(P <0.05或P <0.01);与模型组比较,给药第10、15天,正常组、各给药组大鼠体质量显著增加(P <0.05或P <0.01)。与模型组比较,正常组、各给药组大鼠小肠推进率显著增加(P <0.01)。与模型组比较,各给药组、正常组大鼠胃窦组织iNOS表达显著下调(P <0.05);与模型组比较,多潘立酮组、五磨饮低剂量组大鼠胃窦组织SP表达显著下调(P <0.01);与模型组比较,五磨饮高剂量组大鼠胃窦组织5-HT表达上调(P <0.05)。与模型组比较,正常组、多潘立酮组、柴芍六君子汤组、五磨饮中剂量组大鼠下丘脑i NOS表达显著升高(P <0.05或P <0.01);与模型组比较,正常组、柴芍六君子汤组、五磨饮中剂量组大鼠下丘脑SP表达显著升高(P <0.05或P <0.01)。结论五磨饮对脾虚气滞证FD模型大鼠具有一定的疗效,调节脑-肠轴iNOS、SP、5-HT表达可能是其作用机制之一。
Objective To study the effect of Wumoyin Decoction on FD rats of spleen deficiency and Qi stagnation syndrome. Methods Ten rats were randomly selected as normal group from 70 female SD rats, and other rats were modeled by "chronic restraint + swimming + irregular eating" compound factor method. 48 modeled rats were randomly divided into model group, Domperidone group, Chaishao Liujunzi Decoction group, Wumoyin Decoction high, middle and low dose group, 8 rats in each group. Besides normal group and model group, the other groups of rats were given the corresponding drug intervention for 14 days. The food intake and body weight were recorded, the small intestine propulsion rate was determined, the expression of iNOS, SP, 5-HT in antral tissue and hypothalamus were detected. Results Compared with model group, the food intake of rats in normal group and administration groups were increased significantly on the 5 th, 10 th and 15 th day of administration(P < 0.05 or P < 0.01).Compared with model group, body mass of rats in normal group and Domperidone group were increased significantly on the 5 th day of administration(P < 0.05 or P < 0.01). Compared with model group, body mass of rats in normal group and administration groups were increased significantly on the 10 th and 15 th day of administration(P < 0.05 or P <0.01). Compared with model group, the propulsive rate of small intestine in normal group and administration groups were increased significantly(P < 0.01). The expression of iNOS in gastric antrum tissue of rats in administration groups and normal groups were significantly down-regulated compared with model group(P < 0.05). Compared with model group, SP expression in gastric antrum tissue of Domperidone group and Wumoyin low dose group rats were significantly deed(P < 0.01). Compared with the model group, the expression of 5-HT in gastric antrum tissue of rats in high dose group of Wumoyin was up-regulated(P < 0.05). Compared with model group, the expression of iNOS in hypothalamus of normal group, Domperidone group, Chaishao Liujunzi Decoction group and Wumoyin medium dose group were significantly increased(P < 0.05 or P < 0.01). Compared with model group, the expression of SP in hypothalamus of normal group, Chaishao Liujunzi Decoction group and Wumoyin medium dose group rats was significantly increased(P < 0.05 or P < 0.01). Conclusion Wumoyin Decoction has certain curative effect on FD rats of spleen deficiency and Qi stagnation syndrome, regulating the expression of iNOS, SP and 5-HT in the brain-gut axis may be one of its mechanisms.
Objective To study the effect of Wumoyin Decoction on FD rats of spleen deficiency and Qi stagnation syndrome. Methods Ten rats were randomly selected as normal group from 70 female SD rats, and other rats were modeled by "chronic restraint + swimming + irregular eating" compound factor method. 48 modeled rats were randomly divided into model group, Domperidone group, Chaishao Liujunzi Decoction group, Wumoyin Decoction high, middle and low dose group, 8 rats in each group. Besides normal group and model group, the other groups of rats were given the corresponding drug intervention for 14 days. The food intake and body weight were recorded, the small intestine propulsion rate was determined, the expression of iNOS, SP, 5-HT in antral tissue and hypothalamus were detected. Results Compared with model group, the food intake of rats in normal group and administration groups were increased significantly on the 5 th, 10 th and 15 th day of administration(P < 0.05 or P < 0.01).Compared with model group, body mass of rats in normal group and Domperidone group were increased significantly on the 5 th day of administration(P < 0.05 or P < 0.01). Compared with model group, body mass of rats in normal group and administration groups were increased significantly on the 10 th and 15 th day of administration(P < 0.05 or P <0.01). Compared with model group, the propulsive rate of small intestine in normal group and administration groups were increased significantly(P < 0.01). The expression of iNOS in gastric antrum tissue of rats in administration groups and normal groups were significantly down-regulated compared with model group(P < 0.05). Compared with model group, SP expression in gastric antrum tissue of Domperidone group and Wumoyin low dose group rats were significantly deed(P < 0.01). Compared with the model group, the expression of 5-HT in gastric antrum tissue of rats in high dose group of Wumoyin was up-regulated(P < 0.05). Compared with model group, the expression of iNOS in hypothalamus of normal group, Domperidone group, Chaishao Liujunzi Decoction group and Wumoyin medium dose group were significantly increased(P < 0.05 or P < 0.01). Compared with model group, the expression of SP in hypothalamus of normal group, Chaishao Liujunzi Decoction group and Wumoyin medium dose group rats was significantly increased(P < 0.05 or P < 0.01). Conclusion Wumoyin Decoction has certain curative effect on FD rats of spleen deficiency and Qi stagnation syndrome, regulating the expression of iNOS, SP and 5-HT in the brain-gut axis may be one of its mechanisms.
引文
[1]吴晓芳,冯伟勋,甘国兴,等.五磨饮治疗脾虚气滞型功能性消化不良疗效研究[J].中华中医药学刊,2015,33(3):653-655.
[2]吴晓芳,冯伟勋,甘国兴,等.五磨饮治疗脾虚气滞型功能性消化不良的机制研究[J].中国医药导报,2014,11(27):74-77.
[3]毛兰芳,汪龙德,张宏伟,等.功能性消化不良与脑肠肽的研究进展[J].世界华人消化杂志,2015,25(4):570-576.
[4]高飞,刘铁刚,白辰,等.脑肠轴与胃肠动力之间相关性的研究进展[J].天津中医药大学学报,2018,37(6):520-524.
[5]康朝霞,张红星,唐雷,等.电针对功能性消化不良大鼠胃肠动力及血浆胃饥饿素的影响[J].湖北中医药大学学报,2018,20(5):5-8.
[6]马晓颖,史丽萍,张健.半夏泻心汤联合莫沙必利治疗功能性消化不良疗效及对患者血浆胃动素水平的影响[J].陕西中医,2018,39(10):1399-1401.
[7]李红,曾祥东.四磨汤对肝脾不和型功能性消化不良患者胃肠激素水平的影响[J].中医临床研究,2018,10(6):36-37.
[8]胡柳丹,杨斌,时昭红.不同剂量平胃消导胶囊对功能性消化不良大鼠胃肠动力的影响[J].中国老年学杂志,2018,38(19):4759-4761.
[9]徐派的,杨云,辛玉,等.电针对功能性消化不良肝郁脾虚型大鼠中枢及外周VIP及其受体VPAC-1的影响[J].中华中医药杂志,2016,31(8):3020-3024.
[10]王方方.肝郁脾虚证大鼠十二指肠及下丘脑CCK、CCK1R变化及逍遥散的调节作用[D].北京:北京中医药大学,2018.
[11]林武红,王占有,谢利双,等.加味理中汤联合韦氏整脊手法治疗功能性消化不良脾胃虚寒证临床疗效观察及对患者血清MTL、CCK的影响[J].四川中医,2018,36(9):116-118.
[12]高宏.功能性消化不良患者血浆NPSRI、CGRP及IL-6的表达水平及临床意义[J].中国医学前沿杂志:电子版,2016,8(8):57-59.
[13]马冰芬,曾庆新,邱锋,等.功能性消化不良患者胃动素及降钙素基因相关肽表达水平的变化[J].福建医药杂志,2017,39(4):89-90.
[14]蒋莉,吴长亮,蒙华莹.六味能消丸治疗功能性消化不良效果及其对患者血清NPSR1、CGRP、SP水平的影响[J].山东医药,2018,58(2):54-56.
[15]李艳彦,谢鸣,陈禹,等.一种运用复合病因造模法复制大鼠肝郁脾虚证模型的研究[J].中国中医基础医学杂志,2006,12(6):439-442.
[16]吴晓芳.疏肝健脾活血方治疗肝郁脾虚型FD的临床观察及实验研究[D].广州:广州中医药大学,2017.
[17] Ang D,Pauwels A,Akyuz F,et al. Influence of a neurokinin-1 receptor antagonist(aprepitant)on gastric sensorimotor function in healthy volunteers[J]. Neurogastroenterol Motil,2013,25:e830-e838.PMID:23991829.DOI:10.1111/nmo.12210.
[18]李晓玲,张声生,杨成,等.仁术健脾理气方对功能性消化不良大鼠胃排空功能及Ghrelin、5-HT、CGRP的影响[J].中国中西医结合消化杂志,2014,22(7):355-359.
[19]陈婕,张津玮,杨岩,等.胃泰方对功能性消化不良肝郁脾虚型大鼠一氧化氮、神经降压素及胃窦5-羟色胺表达的影响[J].河北中医,2017,39(7):1064-1068.
[2]吴晓芳,冯伟勋,甘国兴,等.五磨饮治疗脾虚气滞型功能性消化不良的机制研究[J].中国医药导报,2014,11(27):74-77.
[3]毛兰芳,汪龙德,张宏伟,等.功能性消化不良与脑肠肽的研究进展[J].世界华人消化杂志,2015,25(4):570-576.
[4]高飞,刘铁刚,白辰,等.脑肠轴与胃肠动力之间相关性的研究进展[J].天津中医药大学学报,2018,37(6):520-524.
[5]康朝霞,张红星,唐雷,等.电针对功能性消化不良大鼠胃肠动力及血浆胃饥饿素的影响[J].湖北中医药大学学报,2018,20(5):5-8.
[6]马晓颖,史丽萍,张健.半夏泻心汤联合莫沙必利治疗功能性消化不良疗效及对患者血浆胃动素水平的影响[J].陕西中医,2018,39(10):1399-1401.
[7]李红,曾祥东.四磨汤对肝脾不和型功能性消化不良患者胃肠激素水平的影响[J].中医临床研究,2018,10(6):36-37.
[8]胡柳丹,杨斌,时昭红.不同剂量平胃消导胶囊对功能性消化不良大鼠胃肠动力的影响[J].中国老年学杂志,2018,38(19):4759-4761.
[9]徐派的,杨云,辛玉,等.电针对功能性消化不良肝郁脾虚型大鼠中枢及外周VIP及其受体VPAC-1的影响[J].中华中医药杂志,2016,31(8):3020-3024.
[10]王方方.肝郁脾虚证大鼠十二指肠及下丘脑CCK、CCK1R变化及逍遥散的调节作用[D].北京:北京中医药大学,2018.
[11]林武红,王占有,谢利双,等.加味理中汤联合韦氏整脊手法治疗功能性消化不良脾胃虚寒证临床疗效观察及对患者血清MTL、CCK的影响[J].四川中医,2018,36(9):116-118.
[12]高宏.功能性消化不良患者血浆NPSRI、CGRP及IL-6的表达水平及临床意义[J].中国医学前沿杂志:电子版,2016,8(8):57-59.
[13]马冰芬,曾庆新,邱锋,等.功能性消化不良患者胃动素及降钙素基因相关肽表达水平的变化[J].福建医药杂志,2017,39(4):89-90.
[14]蒋莉,吴长亮,蒙华莹.六味能消丸治疗功能性消化不良效果及其对患者血清NPSR1、CGRP、SP水平的影响[J].山东医药,2018,58(2):54-56.
[15]李艳彦,谢鸣,陈禹,等.一种运用复合病因造模法复制大鼠肝郁脾虚证模型的研究[J].中国中医基础医学杂志,2006,12(6):439-442.
[16]吴晓芳.疏肝健脾活血方治疗肝郁脾虚型FD的临床观察及实验研究[D].广州:广州中医药大学,2017.
[17] Ang D,Pauwels A,Akyuz F,et al. Influence of a neurokinin-1 receptor antagonist(aprepitant)on gastric sensorimotor function in healthy volunteers[J]. Neurogastroenterol Motil,2013,25:e830-e838.PMID:23991829.DOI:10.1111/nmo.12210.
[18]李晓玲,张声生,杨成,等.仁术健脾理气方对功能性消化不良大鼠胃排空功能及Ghrelin、5-HT、CGRP的影响[J].中国中西医结合消化杂志,2014,22(7):355-359.
[19]陈婕,张津玮,杨岩,等.胃泰方对功能性消化不良肝郁脾虚型大鼠一氧化氮、神经降压素及胃窦5-羟色胺表达的影响[J].河北中医,2017,39(7):1064-1068.