基于温度响应性纳米复合物对脐疗新制剂及药物释放的研究
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摘要
目的:利用高光热性能的温度响应性纳米复合物对传统脐疗方法进行改进,最终制成可控发热、协同给药及促进透皮吸收的新剂型。方法:运用化学共沉淀法制备Fe3O4纳米颗粒,将其与pNIPAM水凝胶结合,进行扫描电镜(SEM)、光热性能转换、溶胀行为动力学测试,并以盐酸小檗碱为药物模型,对其体外药物释放行为做初步研究,为后续脐疗方载药研究提供一定的参考依据。结果:合成的Fe3O4纳米颗粒较均匀,Fe3O4@pNIPAM复合水凝胶均呈现多孔蜂窝状的形貌结构,均具备良好的光热转换性能,药物释放结果表明在25℃条件下,近红外光能促进Fe3O4@pNIPAM复合水凝胶中盐酸小檗碱的释放,且在42℃时,Fe3O4@pNIPAM复合水凝胶药物累积释放量高于空白pNIPAM水凝胶。结论:Fe3O4@pNIPAM复合水凝胶可作为外用智能透皮给药的载体,且配合近红外光的作用能促进药物释放。
Objective: The traditional umbilical therapy method is improved by using the photoresponsive nanocomposite with high photothermal property, and finally a new dosage form with controlled heat, synergistic drug administration and promoting transdermal absorption is prepared. Methods: Fe3 O4 nanoparticles were prepared by chemical co-precipitation, and it was combined with pNIPAM hydrogel, scanning electron microscopy(SEM), photothermal property conversion and swelling behavior kinetics were tested, and berberine hydrochloride was used as a drug model. The preliminary study on drug release behavior in vitro provided a certain reference for the subsequent drug-loaded study of umbilical therapy. Results: The synthesized Fe3 O4 nanoparticles were relatively uniform, and the Fe3 O4@pNIPAM composite hydrogels exhibited porous honeycomb morphology. It has good photothermal conversion performance, and the drug release results show that near-infrared light can promote the release of berberine hydrochloride in Fe3 O4@pNIPAM composite hydrogel at 25°C. When at 42°C, cumulative release of Fe3 O4@pNIPAM composite hydrogel was higher than that of blank pNIPAM hydrogel. Conclusion: Fe3 O4@pNIPAM composite hydrogel can be used as a carrier for external transdermal drug delivery, and it can promote drug release with the action of near-infrared light.
Objective: The traditional umbilical therapy method is improved by using the photoresponsive nanocomposite with high photothermal property, and finally a new dosage form with controlled heat, synergistic drug administration and promoting transdermal absorption is prepared. Methods: Fe3 O4 nanoparticles were prepared by chemical co-precipitation, and it was combined with pNIPAM hydrogel, scanning electron microscopy(SEM), photothermal property conversion and swelling behavior kinetics were tested, and berberine hydrochloride was used as a drug model. The preliminary study on drug release behavior in vitro provided a certain reference for the subsequent drug-loaded study of umbilical therapy. Results: The synthesized Fe3 O4 nanoparticles were relatively uniform, and the Fe3 O4@pNIPAM composite hydrogels exhibited porous honeycomb morphology. It has good photothermal conversion performance, and the drug release results show that near-infrared light can promote the release of berberine hydrochloride in Fe3 O4@pNIPAM composite hydrogel at 25°C. When at 42°C, cumulative release of Fe3 O4@pNIPAM composite hydrogel was higher than that of blank pNIPAM hydrogel. Conclusion: Fe3 O4@pNIPAM composite hydrogel can be used as a carrier for external transdermal drug delivery, and it can promote drug release with the action of near-infrared light.
引文
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[3]Chu L Y,Xie R,Ju X J,et al.Smart Hydrogel Functional Materials.Berlin,Heidelberg:Springer-Verlag,2013
[4]马晓梅,赵喜安,唐小真.智能型水凝胶.化学通报,2004(2):117-123
[5]Seliktar D.Designing cell-compatible hydrogels for biomedical applications.Science,2012,336:1124-1128
[6]Stuart M A C,Huck W T S,Genzer J,et al.Emerging applications of stimuli-responsive polymer materials.Nat Mater,2010,9:101-113
[7]Chen k,Li J,Feng Y,et al.Structural and rheolgical Characterizations of nanoparticales of environment-sensitive hydrophobic alginate inaqueoussolution.Materials Science and Engineering:C,2017,70:617-627
[8]Zhu J,Guan S,Hu Q,et al.Tough and pH-sensitive hydroxypropyl guar gum/polyacrylamide hybrid double-network hydrogel.Chemical Engineering Journal,2016,306:953-960
[9]高树中.中医脐疗大全.济南:济南出版社,1992:1
[10]张俊洋.近红外光响应性Mo S_2/PNIPAM复合水凝胶的制备与应用.天津:天津大学,2016
[11]张晏宁.掺杂聚多巴胺颗粒近红外响应型智能水凝胶的研究.成都:西南交通大学,2016
[12]Zhang J T,Petersen S,Thunga M,et al.Micro-structured smart hydrogels with enhanced protein loading and release efficiency.Acta Biomater,2010,6(4):1297-1306