基于极大倾角光纤光栅的降钙素原免疫传感器
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摘要
降钙素原(Procalcitonin,PCT)对于反映患者炎症甚至败血症患病程度具有重要意义。为实现低浓度PCT的免标记检测,利用极大倾角光纤光栅(Excessively Tilted Fiber Grating,Ex-TFG)的低温度交叉敏感性和高折射率灵敏度的优点,在光纤的表面修饰金黄色葡萄球菌(Staphylococcus Aureus Protein A,SPA),再将PCT单克隆抗体固定在SPA分子层,从而构成对PCT具有特异性检测功能的免疫传感器。实验结果表明:降钙素原含量为0.5ng/mL时,谐振波长红移约13pm;PCT的浓度为200ng/mL时,Ex-TFG的红移量基达约125pm;浓度为1 000ng/mL时,红移量趋于饱和,最大红移量约150pm。通过对实验数据拟合,发现Ex-TFG谐振波长的红移量与PCT浓度之间的关系满足Langmuir模型,相关性系数为0.98。该方法为低浓度PCT的检测提供了一种免标记、快速的检测方法。
Procalcitonin(PCT)plays an important role as the factor to reflect patients' inflammation or sepsis degree.In order to reach a low concentration detection of PCT with label free method,proposed was a type of PCT immunosensor based on excessively tilted fiber grating(Ex-TFG)which is insensitive to temputere but sensitive to refractive index.Staphylococcus aureus Protein A(SPA)was used to modify the surface of Ex-TFG,then highly purified PCT monoclonal antibodies(MAbs)were immobilized on Ex-TFG's surface through SPA molecules,so as to form the immunosensor which is specific to PCT.Experimental results show that the wavelength shift is about 13 pm when PCT concentration is 0.5 ng/mL,and it becomes to be125 pm with the concentration of PCT increasing to 200 ng/mL.What's more,the immunosensor becomes saturated at~1 000 ng/mL,and the corresponding wavelength shift is about 150 pm.Finally, by fitting the experimental data, it demonstrats that the fitting line meets the Langmuir model function with a corelated coefficient of 0.98.
Procalcitonin(PCT)plays an important role as the factor to reflect patients' inflammation or sepsis degree.In order to reach a low concentration detection of PCT with label free method,proposed was a type of PCT immunosensor based on excessively tilted fiber grating(Ex-TFG)which is insensitive to temputere but sensitive to refractive index.Staphylococcus aureus Protein A(SPA)was used to modify the surface of Ex-TFG,then highly purified PCT monoclonal antibodies(MAbs)were immobilized on Ex-TFG's surface through SPA molecules,so as to form the immunosensor which is specific to PCT.Experimental results show that the wavelength shift is about 13 pm when PCT concentration is 0.5 ng/mL,and it becomes to be125 pm with the concentration of PCT increasing to 200 ng/mL.What's more,the immunosensor becomes saturated at~1 000 ng/mL,and the corresponding wavelength shift is about 150 pm.Finally, by fitting the experimental data, it demonstrats that the fitting line meets the Langmuir model function with a corelated coefficient of 0.98.
引文
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[20]Luo B,Yan Z,Sun Z,et al.Novel glucose sensor based on enzyme-immobilized 81°tilted fiber grating[J].Opt.Express,2014,22(25):30571-30578.
[21]Kangwa M,Yelemane V,Polat A N,et al.High-level fedbatch fermentative expression of an engineered staphylococcal protein A based ligand in E.coli:purification and characterization[J].Amb.Express,2015,5(1):1-10.
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[2]Chauhan R,Solanki P R,Singh J,et al.A novel electrochemical piezoelectric label free immunosensor for aflatoxin B1detection in groundnut[J].Food Control,2015,52:60-70.
[3]Brecht A,Gauglitz G.Optical probes and transducers[J].Biosensors and Bioelectron.,1995,10(9/10):923-936.
[4]Brzozowska E,Koba M,Mietana M,et al.Label-free gramnegative bacteria detection using bacteriophage-adhesin-coated long-period gratings[J].Biomedical Opt.Express,2016,7(3):829-840.
[5]He Z,Tian F,Zhu Y,et al.Long-period gratings in photonic crystal fiber as an optofluidic label-free biosensor[J].Biosensors and Bioelectron.,2011,26(12):4774-4778.
[6]Ju S,Jeong S,Kim Y,et al.Experimental demonstration of surface plasmon resonance enhancement of the tapered optical fiber coated with Au/Ti thin film[J].J.of Non-Crystalline Solids,2014,383(1):146-152.
[7]Im H,Shao H,Park Y I,et al.Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor[J].Nature Biotechnol.,2014,32(5):490-495.
[8]Jang H S,Park K N,Kim J P,et al.Sensitive DNA biosensor based on a long-period grating formed on the sidepolished fiber surface[J].Opt.Express,2009,17(5):3855-3860.
[9]李洪祥.C-反应蛋白,降钙素原与脓毒症的诊断及指导抗生素治疗的临床研究[D].长春:吉林大学,2015.Li Hongxiang.C-reactive protein,procalcitonin and sepsis in the diagnosis and guidance of antibiotic therapy clinical research[D].Changchun:Jilin University,2015.
[10]Wacker C,Prkno A,Brunkhorst F M,et al.Procalcitonin as a diagnostic marker for sepsis:a systematic review and metaanalysis[J].The Lancet Infectious Diseases,2013,13(5):426-435.
[11]Schuetz P,Muller B,Christ-Crain M,et al.Procalcitonin to initiate or discontinue antibiotics in acute respiratory tract infections[J].Evidence-Based Child Health:A Cochrane Rev.J.,2013,8(4):1297-1371.
[12]Schuetz P,Briel M,Mueller B.Clinical outcomes associated with procalcitonin algorithms to guide antibiotic therapy in respiratory tract infections[J].Jama,2013,309(7):717-718.
[13]Liu F,Xiang G,Yuan R,et al.Procalcitonin sensitive detection based on graphene-gold nanocomposite film sensor platform and single-walled carbon nanohorns/hollow Pt chains complex as signal tags[J].Biosensors and Bioelectron.,2014,60(22):210-217.
[14]王霞,陈飞,宿宸铭,等.降钙素原胶体金试纸条显色分析仪[J].光学精密工程,2017,25(3):562-568.Wang Xia,Chen Fei,Su Chenming,et al.Procalcitonin colloidal gold strips color analyzer[J].Opt.and Precision Eng.,2017,25(3):562-568.
[15]饶云江,王义平,朱涛.光纤光栅原理及应用[M].北京:科学出版社,2006.Rao Yunjiang,Wang Yiping,Zhu Tao.Principle and Application of Fiber Grating[M].Beijing:Science Press,2006.
[16]罗彬彬,邹文根,赵明富,等.极大倾斜角度光纤光栅pH值传感器及其增敏研究[J].光学学报,2017(1):105-112.Luo Binbin,Zou Wengen,Zhao Mingfu,et al.Study on fiber grating pH value sensor with maximum tilt angle and sensitivity enhancement[J].Act.Opt.Sin.,2017(1):105-112.
[17]Zhou K,Zhang L,Chen X,et al.Optic sensors of high refractive-index responsivity and low thermal cross sensitivity that use fiber Bragg gratings of>80°tilted structures[J].Opt.Lett.,2006,31(9):1193-1195.
[18]赵明富,邹文根,罗彬彬,等.细直径极大倾角光纤光栅pH值传感器[J].半导体光电,2017,38(3):315-320.Zhao Mingfu,Zou Wengen,Luo Binbin,et al.Thin diameter,maximum inclination fiber grating pH sensor[J].Semiconductor Optoelectronics,2017,38(3):315-320.
[19]赵明富,韩汐,罗彬彬,等.长周期光纤光栅的折射率梯度响应特性[J].光学精密工程,2013,21(2):316-321.Zhao Mingfu,Han Xi,Luo Binbin,et al.The refractive index gradient response characteristic of LPFG[J].Opt.and Precision Eng.,2013,21(2):316-321.
[20]Luo B,Yan Z,Sun Z,et al.Novel glucose sensor based on enzyme-immobilized 81°tilted fiber grating[J].Opt.Express,2014,22(25):30571-30578.
[21]Kangwa M,Yelemane V,Polat A N,et al.High-level fedbatch fermentative expression of an engineered staphylococcal protein A based ligand in E.coli:purification and characterization[J].Amb.Express,2015,5(1):1-10.
[22]张志军,刘炯天.基于Langmuir理论的平衡吸附量预测模型[J].东北大学学报(自然科学版),2011,32(5):749-751.Zhang Zhijun,Liu Jiongtian.A prediction model of equilibrium adsorption capacity based on Langmuir theory[J].J.of Northeastern University(Natural Science Ed.),2011,32(5):749-751.